Mutagenetix > Incidental Mutation

Incidental Mutation 'R4357:Or5w11'

324584

Institutional Source

Beutler Lab

Gene Symbol

Or5w11

Ensembl Gene

ENSMUSG00000068819

Gene Name

olfactory receptor family 5 subfamily W member 11

Synonyms

GA_x6K02T2Q125-49133664-49134593, MOR177-4, Olfr1131

MMRRC Submission

041109-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R4357 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

87458809-87459738 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

T to A at 87458810 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000150754 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090712] [ENSMUST00000213302] [ENSMUST00000216082] [ENSMUST00000216756]

AlphaFold

Q7TR45

Predicted Effect

probably null
Transcript: ENSMUST00000090712
AA Change: M1K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000088214
Gene: ENSMUSG00000068819
AA Change: M1K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	307	5.2e-47	PFAM
Pfam:7tm_1	40	289	1.7e-18	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213302
AA Change: M1K

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Predicted Effect

probably benign
Transcript: ENSMUST00000216082

Predicted Effect

probably benign
Transcript: ENSMUST00000216756

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam20	A	G	8: 41,248,084 (GRCm39)	T65A	possibly damaging	Het
Alpk1	C	T	3: 127,523,022 (GRCm39)	V7M	probably damaging	Het
Ap4m1	A	G	5: 138,171,311 (GRCm39)	E125G	probably damaging	Het
Atp13a2	C	A	4: 140,729,215 (GRCm39)	D599E	probably benign	Het
Brme1	T	C	8: 84,886,221 (GRCm39)	L59P	probably benign	Het
Camkv	G	A	9: 107,825,145 (GRCm39)	G354E	probably benign	Het
Casp8ap2	T	A	4: 32,646,150 (GRCm39)	M1741K	probably benign	Het
Chtf18	T	C	17: 25,938,106 (GRCm39)	D119G	probably benign	Het
Cnga1	T	A	5: 72,775,595 (GRCm39)	D42V	probably damaging	Het
Cysltr2	T	C	14: 73,267,084 (GRCm39)	I209V	probably benign	Het
Defa27	A	T	8: 21,805,608 (GRCm39)	Q16L	probably null	Het
Dnaja3	T	C	16: 4,517,731 (GRCm39)	I321T	probably damaging	Het
Eef2	GCCC	GCCCC	10: 81,014,601 (GRCm39)		probably null	Het
Eif2ak3	T	A	6: 70,861,859 (GRCm39)	I467N	probably damaging	Het
Eif5b	T	A	1: 38,089,339 (GRCm39)	V1105E	probably damaging	Het
Gfod2	T	C	8: 106,444,177 (GRCm39)	N122S	possibly damaging	Het
Gm10803	A	G	2: 93,394,350 (GRCm39)	R41G	unknown	Het
Gm38392	A	T	3: 88,154,741 (GRCm39)	I221N	probably damaging	Het
Grm7	G	T	6: 110,623,309 (GRCm39)	V161F	probably damaging	Het
Hsd17b6	G	T	10: 127,829,637 (GRCm39)		probably null	Het
Ids	C	A	X: 69,389,950 (GRCm39)	G506C	probably damaging	Het
Ifna15	G	T	4: 88,476,079 (GRCm39)	T135N	probably benign	Het
Igsf9b	G	A	9: 27,220,774 (GRCm39)	V47I	possibly damaging	Het
Kif24	A	G	4: 41,413,827 (GRCm39)		probably null	Het
Lats2	A	G	14: 57,936,840 (GRCm39)	S550P	probably damaging	Het
Lmo7	A	G	14: 102,125,091 (GRCm39)	R406G	probably null	Het
Lpcat2	C	A	8: 93,599,734 (GRCm39)	P234Q	probably benign	Het
Lrp10	C	T	14: 54,705,823 (GRCm39)	R338C	probably damaging	Het
Lrrfip2	A	G	9: 111,028,755 (GRCm39)	E326G	probably damaging	Het
Megf8	T	A	7: 25,055,174 (GRCm39)	I1969N	probably benign	Het
Mib1	A	G	18: 10,751,844 (GRCm39)	N242S	probably benign	Het
Ngf	A	G	3: 102,427,521 (GRCm39)	E94G	probably benign	Het
Oas2	C	T	5: 120,876,734 (GRCm39)		probably null	Het
Odf2	A	G	2: 29,782,256 (GRCm39)	T75A	probably benign	Het
Or4f60	A	G	2: 111,902,583 (GRCm39)	L115P	probably damaging	Het
Pi4ka	T	A	16: 17,185,303 (GRCm39)	I266F	probably benign	Het
Prps2	T	A	X: 166,146,545 (GRCm39)	K176*	probably null	Het
Psmd2	T	G	16: 20,475,402 (GRCm39)	D393E	probably benign	Het
Rabep2	A	G	7: 126,047,397 (GRCm39)	I753T	probably damaging	Het
Rara	A	C	11: 98,858,937 (GRCm39)	I129L	probably damaging	Het
Rasgrf2	C	A	13: 92,038,796 (GRCm39)	D1017Y	probably damaging	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Spmip4	T	C	6: 50,551,190 (GRCm39)	T420A	probably benign	Het
Spsb1	T	C	4: 149,991,232 (GRCm39)	H112R	probably damaging	Het
Sptlc1	A	T	13: 53,528,068 (GRCm39)	I32K	probably damaging	Het
Srd5a3	T	A	5: 76,295,547 (GRCm39)	F79Y	probably damaging	Het
Ssrp1	A	T	2: 84,871,495 (GRCm39)	M306L	probably benign	Het
Sulf2	T	C	2: 165,919,497 (GRCm39)	E817G	probably benign	Het
Tiam2	C	A	17: 3,501,128 (GRCm39)	D922E	probably damaging	Het
Tle4	A	G	19: 14,445,625 (GRCm39)	V207A	probably benign	Het
Ttll12	A	T	15: 83,465,958 (GRCm39)	C413S	probably damaging	Het
Usp45	T	A	4: 21,834,350 (GRCm39)	Y809*	probably null	Het
Vmn2r59	G	A	7: 41,661,644 (GRCm39)	P724S	probably damaging	Het
Vrk3	C	T	7: 44,424,866 (GRCm39)	T427M	probably benign	Het

Other mutations in Or5w11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01388:Or5w11	APN	2	87,458,973 (GRCm39)	missense	probably benign	0.03
IGL02186:Or5w11	APN	2	87,459,715 (GRCm39)	missense	probably benign	0.00
R0266:Or5w11	UTSW	2	87,459,626 (GRCm39)	missense	possibly damaging	0.79
R0849:Or5w11	UTSW	2	87,459,626 (GRCm39)	missense	possibly damaging	0.79
R1607:Or5w11	UTSW	2	87,459,321 (GRCm39)	missense	probably benign	0.14
R2077:Or5w11	UTSW	2	87,459,173 (GRCm39)	missense	probably damaging	1.00
R2105:Or5w11	UTSW	2	87,459,283 (GRCm39)	missense	probably benign	0.20
R2322:Or5w11	UTSW	2	87,459,118 (GRCm39)	missense	possibly damaging	0.86
R3434:Or5w11	UTSW	2	87,459,418 (GRCm39)	missense	probably benign	0.02
R5139:Or5w11	UTSW	2	87,459,000 (GRCm39)	missense	probably benign	0.03
R5333:Or5w11	UTSW	2	87,459,458 (GRCm39)	missense	probably damaging	0.97
R5719:Or5w11	UTSW	2	87,459,475 (GRCm39)	splice site	probably null
R6449:Or5w11	UTSW	2	87,459,493 (GRCm39)	missense	possibly damaging	0.63
R7640:Or5w11	UTSW	2	87,459,436 (GRCm39)	missense	probably benign	0.01
R7941:Or5w11	UTSW	2	87,459,248 (GRCm39)	missense	probably benign	0.06
R8110:Or5w11	UTSW	2	87,458,951 (GRCm39)	missense	possibly damaging	0.75
R8393:Or5w11	UTSW	2	87,459,197 (GRCm39)	missense	probably damaging	0.97
R8877:Or5w11	UTSW	2	87,459,212 (GRCm39)	missense	probably damaging	1.00
R9250:Or5w11	UTSW	2	87,459,278 (GRCm39)	missense	probably benign	0.02
R9290:Or5w11	UTSW	2	87,459,209 (GRCm39)	missense	probably benign	0.00
R9313:Or5w11	UTSW	2	87,459,076 (GRCm39)	missense	probably benign	0.03
R9666:Or5w11	UTSW	2	87,459,152 (GRCm39)	missense	possibly damaging	0.78
X0027:Or5w11	UTSW	2	87,459,647 (GRCm39)	missense	possibly damaging	0.96
Z1176:Or5w11	UTSW	2	87,459,659 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGGTGGATAATCTCTGTTCCTC -3'
(R):5'- CACAGAAAGACAGGTGGCTC -3'

Sequencing Primer
(F):5'- TCAACTCTCTATCACAGATACTGG -3'
(R):5'- CTCAGGAAAAAGTACATGGGTGTATG -3'

Posted On

2015-06-24