Mutagenetix > Incidental Mutation

Incidental Mutation 'R4851:Cacna1e'

374528

Institutional Source

Beutler Lab

Gene Symbol

Cacna1e

Ensembl Gene

ENSMUSG00000004110

Gene Name

calcium channel, voltage-dependent, R type, alpha 1E subunit

Synonyms

Cav2.3, Cchra1, alpha1E

MMRRC Submission

042463-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R4851 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

154266477-154760247 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 154312300 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000148507 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004214] [ENSMUST00000187541] [ENSMUST00000211821]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000004214

SMART Domains

Protein: ENSMUSP00000004214
Gene: ENSMUSG00000004110

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	1	55	6.7e-10	PFAM
Pfam:Ion_trans	168	407	3.3e-56	PFAM
Pfam:PKD_channel	257	401	3.3e-7	PFAM
low complexity region	409	414	N/A	INTRINSIC
low complexity region	455	469	N/A	INTRINSIC
low complexity region	496	514	N/A	INTRINSIC
low complexity region	604	620	N/A	INTRINSIC
low complexity region	626	640	N/A	INTRINSIC
coiled coil region	793	823	N/A	INTRINSIC
Pfam:Ion_trans	847	1128	2.3e-63	PFAM
Pfam:Ion_trans	1172	1429	2.6e-65	PFAM
Pfam:PKD_channel	1256	1424	2.8e-10	PFAM
Pfam:GPHH	1431	1500	1.3e-37	PFAM
Ca_chan_IQ	1555	1589	5.93e-13	SMART
low complexity region	1701	1717	N/A	INTRINSIC
low complexity region	1729	1742	N/A	INTRINSIC
low complexity region	1764	1780	N/A	INTRINSIC
low complexity region	1789	1804	N/A	INTRINSIC
low complexity region	1808	1822	N/A	INTRINSIC
low complexity region	1832	1846	N/A	INTRINSIC
low complexity region	1867	1878	N/A	INTRINSIC
low complexity region	1936	1946	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000187541

SMART Domains

Protein: ENSMUSP00000140937
Gene: ENSMUSG00000004110

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	128	351	8.5e-54	PFAM
PDB:4DEX\|B	354	462	6e-36	PDB
Pfam:Ion_trans	511	703	2.2e-46	PFAM
Pfam:PKD_channel	565	710	1.4e-6	PFAM
low complexity region	717	722	N/A	INTRINSIC
low complexity region	763	777	N/A	INTRINSIC
low complexity region	804	822	N/A	INTRINSIC
low complexity region	912	928	N/A	INTRINSIC
low complexity region	934	948	N/A	INTRINSIC
coiled coil region	1101	1131	N/A	INTRINSIC
low complexity region	1162	1175	N/A	INTRINSIC
Pfam:Ion_trans	1191	1425	4.3e-55	PFAM
Pfam:Ion_trans	1515	1725	5.3e-60	PFAM
Pfam:PKD_channel	1565	1732	4.7e-10	PFAM
Ca_chan_IQ	1863	1897	5.93e-13	SMART
low complexity region	2009	2025	N/A	INTRINSIC
low complexity region	2037	2050	N/A	INTRINSIC
low complexity region	2072	2088	N/A	INTRINSIC
low complexity region	2097	2112	N/A	INTRINSIC
low complexity region	2116	2130	N/A	INTRINSIC
low complexity region	2140	2154	N/A	INTRINSIC
low complexity region	2175	2186	N/A	INTRINSIC
low complexity region	2244	2254	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000211821

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

100% (99/99)

MGI Phenotype

FUNCTION: This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit altered R-type Ca2+ channels, increased timidity and body weight, impaired glucose tolerance, reduced locomotor activity, and lack of the cocaine stimulation of locomotor response. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, knock-out(3) Targeted, other(3) Gene trapped(1)

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,433,890 (GRCm39)	R4148S	probably benign	Het
Acoxl	T	A	2: 127,886,311 (GRCm39)	L182Q	possibly damaging	Het
Aldh7a1	T	C	18: 56,665,088 (GRCm39)	T364A	possibly damaging	Het
App	T	C	16: 84,853,322 (GRCm39)	D252G	unknown	Het
Arid1a	A	G	4: 133,408,672 (GRCm39)	I1945T	unknown	Het
Arsk	C	T	13: 76,213,398 (GRCm39)		probably null	Het
Atad2b	G	A	12: 4,993,251 (GRCm39)	G257S	probably benign	Het
Best1	C	A	19: 9,969,062 (GRCm39)	R218L	probably damaging	Het
Cdh13	C	T	8: 119,484,129 (GRCm39)	T130I	possibly damaging	Het
Cela2a	T	C	4: 141,552,902 (GRCm39)	E25G	probably benign	Het
Cgnl1	T	A	9: 71,632,314 (GRCm39)	I346F	probably damaging	Het
Chil6	C	T	3: 106,297,244 (GRCm39)	G299D	possibly damaging	Het
Cic	G	A	7: 24,972,327 (GRCm39)	R686H	probably damaging	Het
Cnpy1	T	C	5: 28,450,738 (GRCm39)	I23V	probably benign	Het
Col6a3	T	A	1: 90,707,011 (GRCm39)	Y2034F	unknown	Het
Coq10b	T	C	1: 55,110,903 (GRCm39)	Y224H	probably benign	Het
Cryge	G	T	1: 65,090,211 (GRCm39)		probably benign	Het
Cyb5rl	T	G	4: 106,941,510 (GRCm39)	S252A	probably benign	Het
Cyp2ab1	T	A	16: 20,133,814 (GRCm39)	R125S	probably damaging	Het
Dclk1	G	A	3: 55,387,811 (GRCm39)	G86R	probably damaging	Het
Dmtn	A	G	14: 70,842,254 (GRCm39)	M382T	probably damaging	Het
Dnaaf8	A	G	16: 4,794,138 (GRCm39)		noncoding transcript	Het
Dnah12	T	A	14: 26,437,784 (GRCm39)	L471*	probably null	Het
Dock10	A	T	1: 80,526,874 (GRCm39)	S782T	probably benign	Het
Egfem1	C	A	3: 29,206,032 (GRCm39)	H90N	possibly damaging	Het
Ephb6	T	A	6: 41,595,079 (GRCm39)	W698R	probably benign	Het
Exoc4	T	A	6: 33,895,343 (GRCm39)	C787S	probably damaging	Het
Exosc8	A	T	3: 54,639,523 (GRCm39)		probably benign	Het
Galc	T	A	12: 98,193,533 (GRCm39)	Q352L	probably benign	Het
Galns	A	G	8: 123,327,272 (GRCm39)	Y167H	probably damaging	Het
Glb1l	T	C	1: 75,185,528 (GRCm39)		probably benign	Het
Gm6185	A	T	1: 161,033,750 (GRCm39)		noncoding transcript	Het
Gm7694	T	C	1: 170,128,794 (GRCm39)	N245S	probably benign	Het
Gmcl1	T	G	6: 86,681,538 (GRCm39)	K385N	possibly damaging	Het
Gramd1c	A	T	16: 43,810,200 (GRCm39)	W463R	probably damaging	Het
Gtpbp8	C	G	16: 44,566,433 (GRCm39)	A90P	probably benign	Het
H2bc11	G	T	13: 22,227,421 (GRCm39)		probably benign	Het
Hcar1	T	C	5: 124,016,731 (GRCm39)	E320G	probably benign	Het
Hhatl	T	C	9: 121,618,077 (GRCm39)	D226G	probably damaging	Het
Hoxc5	T	A	15: 102,923,801 (GRCm39)	I199N	probably damaging	Het
Iars2	T	C	1: 185,059,845 (GRCm39)	D121G	probably damaging	Het
Il5ra	T	A	6: 106,715,432 (GRCm39)	H134L	probably benign	Het
Isl2	T	A	9: 55,452,271 (GRCm39)	I281N	possibly damaging	Het
Kcnk13	T	C	12: 99,932,383 (GRCm39)	F60L	probably damaging	Het
Kdelr3	C	T	15: 79,409,066 (GRCm39)	T85M	possibly damaging	Het
Kdf1	A	T	4: 133,255,676 (GRCm39)	H131L	probably damaging	Het
Mipol1	T	A	12: 57,379,087 (GRCm39)	L182I	probably damaging	Het
Morc1	T	G	16: 48,381,980 (GRCm39)	S513R	probably benign	Het
Moxd2	A	T	6: 40,855,756 (GRCm39)	L611H	probably damaging	Het
Mpped2	T	C	2: 106,529,724 (GRCm39)		probably benign	Het
Mrpl51	T	C	6: 125,170,270 (GRCm39)	V92A	probably benign	Het
Mrps26	G	A	2: 130,405,681 (GRCm39)		probably benign	Het
Mrps5	A	G	2: 127,432,665 (GRCm39)	T29A	probably benign	Het
Mustn1	A	G	14: 30,601,517 (GRCm39)		probably benign	Het
Ncor2	G	A	5: 125,110,431 (GRCm39)	P858S	possibly damaging	Het
Nfasc	A	G	1: 132,529,759 (GRCm39)	F807S	probably damaging	Het
Nup50	G	A	15: 84,823,912 (GRCm39)	V422I	probably benign	Het
Or10x1	T	A	1: 174,196,562 (GRCm39)	F26L	probably benign	Het
Or13a25	A	T	7: 140,247,226 (GRCm39)	M2L	probably benign	Het
Or1e25	A	T	11: 73,493,883 (GRCm39)	H159L	probably damaging	Het
Or2ag13	A	T	7: 106,473,221 (GRCm39)	V77D	probably damaging	Het
Or3a1d	C	A	11: 74,237,769 (GRCm39)	V214L	probably benign	Het
Or4c107	A	G	2: 88,788,930 (GRCm39)	N40S	probably damaging	Het
Or8b44	T	A	9: 38,410,319 (GRCm39)	M118K	probably damaging	Het
Or8u9	T	A	2: 86,002,015 (GRCm39)	I49F	probably damaging	Het
Osbpl8	T	A	10: 111,040,661 (GRCm39)	M1K	probably null	Het
Pcdhb22	A	C	18: 37,652,087 (GRCm39)	D185A	possibly damaging	Het
Pcdhga8	G	T	18: 37,949,457 (GRCm39)	R291L	probably damaging	Het
Saysd1	A	T	14: 20,127,672 (GRCm39)	L84Q	possibly damaging	Het
Slc41a1	T	C	1: 131,758,508 (GRCm39)	I50T	probably benign	Het
Smg7	G	A	1: 152,720,020 (GRCm39)	P834S	probably benign	Het
Speer4a1	C	T	5: 26,243,210 (GRCm39)	V92M	probably damaging	Het
Srf	T	C	17: 46,860,400 (GRCm39)	T461A	probably benign	Het
Ssu72	A	G	4: 155,800,053 (GRCm39)	D72G	possibly damaging	Het
Suco	T	C	1: 161,661,761 (GRCm39)	E890G	probably damaging	Het
Tenm3	A	G	8: 48,763,656 (GRCm39)		probably null	Het
Tm6sf1	A	G	7: 81,515,091 (GRCm39)	E7G	probably null	Het
Tm9sf2	A	G	14: 122,378,616 (GRCm39)	K240R	probably benign	Het
Tmem52	T	A	4: 155,554,825 (GRCm39)	Y149*	probably null	Het
Tpgs2	A	T	18: 25,284,305 (GRCm39)	Y68N	possibly damaging	Het
Vmn1r205	T	A	13: 22,777,074 (GRCm39)	E9D	probably benign	Het
Vmn2r32	A	G	7: 7,482,953 (GRCm39)	V7A	possibly damaging	Het
Vmn2r80	T	C	10: 79,030,156 (GRCm39)	Y661H	possibly damaging	Het
Vps39	G	A	2: 120,152,312 (GRCm39)		probably benign	Het
Vwde	A	T	6: 13,196,047 (GRCm39)	V326D	possibly damaging	Het
Zfp641	T	A	15: 98,186,598 (GRCm39)	S342C	probably damaging	Het
Zfp961	T	C	8: 72,722,847 (GRCm39)		probably benign	Het

Other mutations in Cacna1e

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00551:Cacna1e	APN	1	154,279,429 (GRCm39)	missense	probably damaging	0.99
IGL01086:Cacna1e	APN	1	154,347,347 (GRCm39)	missense	probably benign	0.04
IGL01302:Cacna1e	APN	1	154,319,653 (GRCm39)	missense	probably damaging	1.00
IGL01386:Cacna1e	APN	1	154,348,123 (GRCm39)	missense	probably benign	0.18
IGL01573:Cacna1e	APN	1	154,347,113 (GRCm39)	missense	probably benign
IGL01676:Cacna1e	APN	1	154,288,196 (GRCm39)	missense	probably damaging	1.00
IGL01676:Cacna1e	APN	1	154,274,222 (GRCm39)	missense	probably damaging	1.00
IGL01762:Cacna1e	APN	1	154,347,119 (GRCm39)	missense	possibly damaging	0.78
IGL01801:Cacna1e	APN	1	154,347,086 (GRCm39)	missense	probably null	0.00
IGL01895:Cacna1e	APN	1	154,319,646 (GRCm39)	missense	probably damaging	1.00
IGL02391:Cacna1e	APN	1	154,296,859 (GRCm39)	missense	probably damaging	1.00
IGL02399:Cacna1e	APN	1	154,279,493 (GRCm39)	missense	probably damaging	1.00
IGL02659:Cacna1e	APN	1	154,302,274 (GRCm39)	missense	probably damaging	1.00
IGL02686:Cacna1e	APN	1	154,369,155 (GRCm39)	missense	probably damaging	1.00
IGL02838:Cacna1e	APN	1	154,321,394 (GRCm39)	missense	probably damaging	1.00
IGL02958:Cacna1e	APN	1	154,341,487 (GRCm39)	missense	probably damaging	1.00
IGL02981:Cacna1e	APN	1	154,347,171 (GRCm39)	missense	probably benign	0.15
IGL03120:Cacna1e	APN	1	154,319,627 (GRCm39)	missense	probably damaging	1.00
IGL03232:Cacna1e	APN	1	154,369,104 (GRCm39)	missense	probably damaging	1.00
IGL03310:Cacna1e	APN	1	154,317,997 (GRCm39)	missense	probably damaging	1.00
IGL03342:Cacna1e	APN	1	154,342,690 (GRCm39)	critical splice donor site	probably null
bezoar	UTSW	1	154,312,300 (GRCm39)	splice site	probably null
hairball	UTSW	1	154,355,051 (GRCm39)	missense	probably damaging	0.97
N/A - 535:Cacna1e	UTSW	1	154,341,510 (GRCm39)	missense	probably damaging	1.00
R0122:Cacna1e	UTSW	1	154,319,647 (GRCm39)	missense	probably damaging	1.00
R0143:Cacna1e	UTSW	1	154,324,693 (GRCm39)	splice site	probably null
R0314:Cacna1e	UTSW	1	154,317,997 (GRCm39)	missense	probably damaging	1.00
R0366:Cacna1e	UTSW	1	154,291,884 (GRCm39)	missense	probably benign	0.03
R0626:Cacna1e	UTSW	1	154,364,563 (GRCm39)	missense	probably damaging	0.99
R0739:Cacna1e	UTSW	1	154,318,024 (GRCm39)	missense	probably damaging	0.97
R1272:Cacna1e	UTSW	1	154,320,714 (GRCm39)	missense	probably damaging	1.00
R1300:Cacna1e	UTSW	1	154,274,419 (GRCm39)	missense	probably benign
R1340:Cacna1e	UTSW	1	154,348,403 (GRCm39)	missense	probably damaging	1.00
R1440:Cacna1e	UTSW	1	154,437,552 (GRCm39)	missense	possibly damaging	0.63
R1449:Cacna1e	UTSW	1	154,361,408 (GRCm39)	critical splice donor site	probably null
R1538:Cacna1e	UTSW	1	154,437,504 (GRCm39)	missense	probably damaging	0.99
R1542:Cacna1e	UTSW	1	154,353,525 (GRCm39)	missense	probably benign	0.01
R1560:Cacna1e	UTSW	1	154,296,850 (GRCm39)	nonsense	probably null
R1748:Cacna1e	UTSW	1	154,362,315 (GRCm39)	missense	possibly damaging	0.92
R1749:Cacna1e	UTSW	1	154,319,746 (GRCm39)	missense	probably damaging	1.00
R1912:Cacna1e	UTSW	1	154,312,195 (GRCm39)	missense	probably damaging	1.00
R1968:Cacna1e	UTSW	1	154,576,240 (GRCm39)	missense	probably damaging	1.00
R1993:Cacna1e	UTSW	1	154,353,563 (GRCm39)	missense	probably damaging	0.97
R1994:Cacna1e	UTSW	1	154,353,563 (GRCm39)	missense	probably damaging	0.97
R2191:Cacna1e	UTSW	1	154,319,591 (GRCm39)	missense	probably damaging	1.00
R2291:Cacna1e	UTSW	1	154,279,429 (GRCm39)	missense	probably damaging	0.99
R2417:Cacna1e	UTSW	1	154,347,939 (GRCm39)	missense	probably damaging	1.00
R3608:Cacna1e	UTSW	1	154,291,831 (GRCm39)	missense	probably benign	0.08
R3757:Cacna1e	UTSW	1	154,509,442 (GRCm39)	missense	probably damaging	0.97
R3890:Cacna1e	UTSW	1	154,359,299 (GRCm39)	missense	probably damaging	1.00
R4015:Cacna1e	UTSW	1	154,358,331 (GRCm39)	missense	probably damaging	1.00
R4088:Cacna1e	UTSW	1	154,287,929 (GRCm39)	splice site	probably null
R4275:Cacna1e	UTSW	1	154,369,071 (GRCm39)	missense	probably damaging	1.00
R4282:Cacna1e	UTSW	1	154,302,296 (GRCm39)	missense	probably benign	0.04
R4297:Cacna1e	UTSW	1	154,274,477 (GRCm39)	missense	probably benign	0.37
R4356:Cacna1e	UTSW	1	154,319,727 (GRCm39)	missense	probably damaging	1.00
R4510:Cacna1e	UTSW	1	154,437,579 (GRCm39)	missense	probably damaging	1.00
R4511:Cacna1e	UTSW	1	154,437,579 (GRCm39)	missense	probably damaging	1.00
R4577:Cacna1e	UTSW	1	154,277,773 (GRCm39)	missense	possibly damaging	0.92
R4590:Cacna1e	UTSW	1	154,312,265 (GRCm39)	missense	possibly damaging	0.87
R4601:Cacna1e	UTSW	1	154,347,359 (GRCm39)	missense	probably benign
R4622:Cacna1e	UTSW	1	154,347,311 (GRCm39)	missense	possibly damaging	0.81
R4626:Cacna1e	UTSW	1	154,358,294 (GRCm39)	splice site	probably null
R4694:Cacna1e	UTSW	1	154,313,012 (GRCm39)	critical splice donor site	probably null
R4727:Cacna1e	UTSW	1	154,312,214 (GRCm39)	nonsense	probably null
R4839:Cacna1e	UTSW	1	154,296,804 (GRCm39)	missense	probably damaging	1.00
R4894:Cacna1e	UTSW	1	154,364,551 (GRCm39)	nonsense	probably null
R4934:Cacna1e	UTSW	1	154,357,380 (GRCm39)	nonsense	probably null
R4979:Cacna1e	UTSW	1	154,289,739 (GRCm39)	missense	probably damaging	1.00
R5077:Cacna1e	UTSW	1	154,437,475 (GRCm39)	critical splice donor site	probably null
R5128:Cacna1e	UTSW	1	154,277,767 (GRCm39)	missense	probably damaging	0.98
R5214:Cacna1e	UTSW	1	154,577,110 (GRCm39)	missense	possibly damaging	0.93
R5274:Cacna1e	UTSW	1	154,576,250 (GRCm39)	missense	probably damaging	0.98
R5388:Cacna1e	UTSW	1	154,353,542 (GRCm39)	missense	probably damaging	1.00
R5416:Cacna1e	UTSW	1	154,341,525 (GRCm39)	missense	probably damaging	1.00
R5469:Cacna1e	UTSW	1	154,319,683 (GRCm39)	missense	probably damaging	1.00
R5475:Cacna1e	UTSW	1	154,601,455 (GRCm39)	missense	possibly damaging	0.53
R5607:Cacna1e	UTSW	1	154,347,086 (GRCm39)	missense	probably benign	0.00
R5615:Cacna1e	UTSW	1	154,287,916 (GRCm39)	missense	probably damaging	1.00
R5616:Cacna1e	UTSW	1	154,317,940 (GRCm39)	missense	probably damaging	1.00
R5627:Cacna1e	UTSW	1	154,511,604 (GRCm39)	missense	probably damaging	0.98
R5707:Cacna1e	UTSW	1	154,509,463 (GRCm39)	missense	probably damaging	1.00
R5756:Cacna1e	UTSW	1	154,347,383 (GRCm39)	missense	probably benign	0.00
R5893:Cacna1e	UTSW	1	154,313,069 (GRCm39)	missense	probably damaging	1.00
R6117:Cacna1e	UTSW	1	154,437,537 (GRCm39)	missense	possibly damaging	0.68
R6134:Cacna1e	UTSW	1	154,577,037 (GRCm39)	missense	probably damaging	1.00
R6190:Cacna1e	UTSW	1	154,362,316 (GRCm39)	missense	possibly damaging	0.47
R6279:Cacna1e	UTSW	1	154,301,678 (GRCm39)	missense	probably benign	0.38
R6295:Cacna1e	UTSW	1	154,317,919 (GRCm39)	missense	probably damaging	0.98
R6300:Cacna1e	UTSW	1	154,301,678 (GRCm39)	missense	probably benign	0.38
R6320:Cacna1e	UTSW	1	154,317,270 (GRCm39)	missense	possibly damaging	0.76
R6375:Cacna1e	UTSW	1	154,355,051 (GRCm39)	missense	probably damaging	0.97
R6830:Cacna1e	UTSW	1	154,289,720 (GRCm39)	critical splice donor site	probably null
R6842:Cacna1e	UTSW	1	154,358,863 (GRCm39)	missense	probably damaging	1.00
R7023:Cacna1e	UTSW	1	154,601,439 (GRCm39)	missense	probably null	0.85
R7081:Cacna1e	UTSW	1	154,576,129 (GRCm39)	missense	possibly damaging	0.82
R7085:Cacna1e	UTSW	1	154,349,492 (GRCm39)	splice site	probably null
R7108:Cacna1e	UTSW	1	154,344,741 (GRCm39)	frame shift	probably null
R7142:Cacna1e	UTSW	1	154,288,230 (GRCm39)	missense	probably damaging	1.00
R7250:Cacna1e	UTSW	1	154,576,235 (GRCm39)	missense	possibly damaging	0.93
R7332:Cacna1e	UTSW	1	154,601,547 (GRCm39)	missense	possibly damaging	0.89
R7410:Cacna1e	UTSW	1	154,347,980 (GRCm39)	missense	probably benign	0.13
R7502:Cacna1e	UTSW	1	154,344,734 (GRCm39)	missense	probably null	0.35
R7556:Cacna1e	UTSW	1	154,348,419 (GRCm39)	missense	probably benign	0.28
R7563:Cacna1e	UTSW	1	154,347,162 (GRCm39)	missense	probably benign	0.00
R7573:Cacna1e	UTSW	1	154,601,911 (GRCm39)	intron	probably benign
R7689:Cacna1e	UTSW	1	154,274,549 (GRCm39)	missense	probably benign	0.01
R7699:Cacna1e	UTSW	1	154,319,674 (GRCm39)	missense	probably damaging	1.00
R7744:Cacna1e	UTSW	1	154,341,538 (GRCm39)	missense	probably damaging	1.00
R7754:Cacna1e	UTSW	1	154,288,863 (GRCm39)	missense	probably damaging	0.97
R7787:Cacna1e	UTSW	1	154,358,314 (GRCm39)	missense	probably damaging	0.98
R7818:Cacna1e	UTSW	1	154,274,152 (GRCm39)	missense	probably damaging	1.00
R7838:Cacna1e	UTSW	1	154,347,149 (GRCm39)	missense	probably benign	0.08
R7849:Cacna1e	UTSW	1	154,509,464 (GRCm39)	missense	probably damaging	1.00
R8011:Cacna1e	UTSW	1	154,341,568 (GRCm39)	missense	probably benign	0.01
R8094:Cacna1e	UTSW	1	154,437,516 (GRCm39)	missense	probably damaging	1.00
R8162:Cacna1e	UTSW	1	154,577,313 (GRCm39)	splice site	probably null
R8202:Cacna1e	UTSW	1	154,274,195 (GRCm39)	missense	probably benign
R8280:Cacna1e	UTSW	1	154,344,839 (GRCm39)	missense	probably damaging	0.97
R8354:Cacna1e	UTSW	1	154,274,314 (GRCm39)	missense	probably damaging	1.00
R8385:Cacna1e	UTSW	1	154,319,687 (GRCm39)	missense	probably damaging	0.98
R8532:Cacna1e	UTSW	1	154,341,510 (GRCm39)	missense	probably damaging	1.00
R8902:Cacna1e	UTSW	1	154,349,632 (GRCm39)	missense	probably benign	0.01
R8926:Cacna1e	UTSW	1	154,577,080 (GRCm39)	missense	possibly damaging	0.84
R8947:Cacna1e	UTSW	1	154,277,896 (GRCm39)	missense	probably benign	0.10
R9094:Cacna1e	UTSW	1	154,355,064 (GRCm39)	missense	possibly damaging	0.93
R9126:Cacna1e	UTSW	1	154,343,510 (GRCm39)	missense	probably benign	0.01
R9175:Cacna1e	UTSW	1	154,274,314 (GRCm39)	missense	probably damaging	1.00
R9286:Cacna1e	UTSW	1	154,288,845 (GRCm39)	missense	probably damaging	1.00
R9377:Cacna1e	UTSW	1	154,361,458 (GRCm39)	missense	possibly damaging	0.88
R9452:Cacna1e	UTSW	1	154,289,720 (GRCm39)	critical splice donor site	probably null
R9463:Cacna1e	UTSW	1	154,357,411 (GRCm39)	missense	probably damaging	1.00
R9513:Cacna1e	UTSW	1	154,318,033 (GRCm39)	missense	probably damaging	1.00
R9534:Cacna1e	UTSW	1	154,320,693 (GRCm39)	missense	possibly damaging	0.65
R9562:Cacna1e	UTSW	1	154,283,486 (GRCm39)	missense	probably benign	0.01
RF008:Cacna1e	UTSW	1	154,317,882 (GRCm39)	missense	probably damaging	1.00
X0062:Cacna1e	UTSW	1	154,288,238 (GRCm39)	missense	probably damaging	1.00
Z1176:Cacna1e	UTSW	1	154,511,596 (GRCm39)	missense	probably damaging	0.98
Z1177:Cacna1e	UTSW	1	154,318,038 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCCTTCCTGGAAGTGAAGG -3'
(R):5'- TAGGACTCAAGGAGCTCAGCTG -3'

Sequencing Primer
(F):5'- GCCGCTGCAGTGTTGTC -3'
(R):5'- CTCAGCTGGGAGGCTTAGAG -3'

Posted On

2016-03-16