Mutagenetix > Incidental Mutation

Incidental Mutation 'R6001:Zfp35'

480852

Institutional Source

Beutler Lab

Gene Symbol

Zfp35

Ensembl Gene

ENSMUSG00000063281

Gene Name

zinc finger protein 35

Synonyms

Zfp-35

MMRRC Submission

044180-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R6001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

24122689-24138433 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 24135816 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 53 (H53Q)

Ref Sequence

ENSEMBL: ENSMUSP00000074475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074941]

AlphaFold

P15620

Predicted Effect

probably benign
Transcript: ENSMUST00000074941
AA Change: H53Q

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000074475
Gene: ENSMUSG00000063281
AA Change: H53Q

Domain	Start	End	E-Value	Type
ZnF_C2H2	78	100	1.28e-3	SMART
ZnF_C2H2	104	126	8.47e-4	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	1.84e-4	SMART
ZnF_C2H2	188	210	7.37e-4	SMART
ZnF_C2H2	216	238	4.72e-2	SMART
ZnF_C2H2	244	266	2.4e-3	SMART
ZnF_C2H2	272	294	1.04e-3	SMART
ZnF_C2H2	300	322	3.69e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	1.5e-4	SMART
ZnF_C2H2	384	406	1.6e-4	SMART
ZnF_C2H2	412	434	3.89e-3	SMART
ZnF_C2H2	440	462	1.72e-4	SMART
ZnF_C2H2	468	490	2.71e-2	SMART
ZnF_C2H2	496	518	1.6e-4	SMART
ZnF_C2H2	524	545	2.17e1	SMART
ZnF_C2H2	551	573	1.6e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out mutation exhibit increased airway responsiveness to ovalbumin and methacholine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca1	G	T	4: 53,075,555 (GRCm39)	R999S	possibly damaging	Het
Ankrd31	T	A	13: 96,962,717 (GRCm39)	Y503N	probably damaging	Het
Anks4b	A	G	7: 119,781,941 (GRCm39)	E324G	probably benign	Het
Arhgap44	CTGCT	CTGCTTGCT	11: 64,922,910 (GRCm39)		probably null	Het
Atl1	A	T	12: 69,979,057 (GRCm39)	T162S	possibly damaging	Het
Atp2b2	A	G	6: 113,770,728 (GRCm39)	Y394H	probably damaging	Het
Dennd2d	A	G	3: 106,399,776 (GRCm39)	H233R	probably benign	Het
Dhx16	G	T	17: 36,194,766 (GRCm39)	M462I	probably damaging	Het
Hif3a	T	C	7: 16,784,486 (GRCm39)	Y253C	probably damaging	Het
Hsf4	G	A	8: 105,999,541 (GRCm39)	G277R	possibly damaging	Het
Impg1	T	C	9: 80,223,454 (GRCm39)	D754G	probably benign	Het
Keap1	T	C	9: 21,142,135 (GRCm39)	S580G	possibly damaging	Het
Lrp6	T	C	6: 134,441,481 (GRCm39)	K1162E	probably benign	Het
Lrrc31	C	T	3: 30,745,318 (GRCm39)	V110I	possibly damaging	Het
Muc5b	G	T	7: 141,426,118 (GRCm39)	K4738N	possibly damaging	Het
Myo1a	G	T	10: 127,542,794 (GRCm39)		probably null	Het
Odad2	T	C	18: 7,286,838 (GRCm39)	D131G	probably benign	Het
Or10q1b	A	G	19: 13,682,424 (GRCm39)	T78A	probably damaging	Het
Or52z14	T	A	7: 103,253,179 (GRCm39)	M106K	probably damaging	Het
Or7g19	A	T	9: 18,856,340 (GRCm39)	Y132F	probably damaging	Het
Parp4	A	T	14: 56,878,740 (GRCm39)	H1225L	probably benign	Het
Pcgf2	T	C	11: 97,583,606 (GRCm39)	Y52C	possibly damaging	Het
Pkn3	T	C	2: 29,978,596 (GRCm39)		probably null	Het
Psen2	C	A	1: 180,073,234 (GRCm39)	R29L	possibly damaging	Het
Rfx6	A	G	10: 51,594,307 (GRCm39)		probably null	Het
Rps13	T	C	7: 115,930,808 (GRCm39)	T145A	probably benign	Het
Rsf1	G	A	7: 97,229,117 (GRCm39)		probably benign	Het
Rsf1	GCG	GCGACG	7: 97,229,114 (GRCm39)		probably benign	Het
Rsf1	A	ACGGCGACGG	7: 97,229,111 (GRCm39)		probably null	Het
Smarca4	C	T	9: 21,544,205 (GRCm39)		probably benign	Het
Stat4	A	T	1: 52,136,026 (GRCm39)	E445V	probably damaging	Het
Taf13	T	A	3: 108,488,387 (GRCm39)	I90N	probably damaging	Het
Tas2r124	T	A	6: 132,732,416 (GRCm39)	Y242N	probably damaging	Het
Tmem151b	T	C	17: 45,856,711 (GRCm39)	Y243C	probably damaging	Het
Wasl	T	C	6: 24,619,573 (GRCm39)	T316A	unknown	Het
Zbtb44	T	C	9: 30,965,090 (GRCm39)	C167R	probably damaging	Het
Zc3h7b	T	C	15: 81,676,236 (GRCm39)	L714P	possibly damaging	Het
Zfp804b	A	G	5: 6,819,043 (GRCm39)	V1340A	probably benign	Het

Other mutations in Zfp35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0012:Zfp35	UTSW	18	24,136,001 (GRCm39)	missense	probably benign	0.01
R1453:Zfp35	UTSW	18	24,136,557 (GRCm39)	nonsense	probably null
R1807:Zfp35	UTSW	18	24,136,986 (GRCm39)	missense	probably benign	0.28
R2317:Zfp35	UTSW	18	24,136,555 (GRCm39)	missense	probably damaging	1.00
R2863:Zfp35	UTSW	18	24,137,352 (GRCm39)	missense	probably damaging	0.99
R3080:Zfp35	UTSW	18	24,136,367 (GRCm39)	missense	probably damaging	0.99
R3756:Zfp35	UTSW	18	24,135,982 (GRCm39)	missense	possibly damaging	0.88
R4114:Zfp35	UTSW	18	24,135,766 (GRCm39)	missense	probably benign	0.08
R4222:Zfp35	UTSW	18	24,136,246 (GRCm39)	missense	possibly damaging	0.91
R4780:Zfp35	UTSW	18	24,136,326 (GRCm39)	missense	probably damaging	1.00
R5137:Zfp35	UTSW	18	24,137,194 (GRCm39)	nonsense	probably null
R5261:Zfp35	UTSW	18	24,136,778 (GRCm39)	missense	probably damaging	0.98
R5375:Zfp35	UTSW	18	24,135,973 (GRCm39)	missense	possibly damaging	0.83
R6190:Zfp35	UTSW	18	24,137,118 (GRCm39)	missense	probably benign	0.00
R6774:Zfp35	UTSW	18	24,136,015 (GRCm39)	missense	possibly damaging	0.51
R6850:Zfp35	UTSW	18	24,135,839 (GRCm39)	missense	possibly damaging	0.76
R6979:Zfp35	UTSW	18	24,136,927 (GRCm39)	missense	probably benign	0.32
R7029:Zfp35	UTSW	18	24,136,583 (GRCm39)	missense	probably damaging	0.98
R7079:Zfp35	UTSW	18	24,136,357 (GRCm39)	missense	possibly damaging	0.89
R7858:Zfp35	UTSW	18	24,136,897 (GRCm39)	missense	probably damaging	1.00
R8257:Zfp35	UTSW	18	24,137,288 (GRCm39)	missense	possibly damaging	0.55
R9477:Zfp35	UTSW	18	24,136,188 (GRCm39)	missense	possibly damaging	0.86
R9642:Zfp35	UTSW	18	24,137,155 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCTGGAGCTTTCTTACAACATGG -3'
(R):5'- GGGCTTCTCTCCAGTATGAATTCTC -3'

Sequencing Primer
(F):5'- GAGCTTTCTTACAACATGGAGCGC -3'
(R):5'- TTCTCTGATGCAGAACCAGG -3'

Posted On

2017-06-26