Mutagenetix > Incidental Mutation

Incidental Mutation 'R6061:Vmn2r85'

483244

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

044226-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R6061 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130253658-130266615 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 130261531 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 269 (I269V)

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably benign
Transcript: ENSMUST00000171811
AA Change: I269V

PolyPhen 2 Score 0.092 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048
AA Change: I269V

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	C	6: 128,545,675 (GRCm39)	F484C	probably damaging	Het
Aoc1l1	A	C	6: 48,953,535 (GRCm39)	I487L	probably benign	Het
Cdsn	A	G	17: 35,865,803 (GRCm39)	S111G	unknown	Het
Ctnnal1	T	C	4: 56,812,349 (GRCm39)	T726A	probably benign	Het
Cyp2j11	T	A	4: 96,236,853 (GRCm39)		probably benign	Het
Ddx60	T	A	8: 62,476,275 (GRCm39)	M1541K	probably null	Het
Dnah14	C	T	1: 181,536,616 (GRCm39)	P2420S	probably damaging	Het
Fhit	T	C	14: 9,573,435 (GRCm38)	E205G	probably benign	Het
Glipr1l1	C	T	10: 111,912,075 (GRCm39)	T203M	probably benign	Het
Gm4353	T	A	7: 115,683,504 (GRCm39)	D97V	probably benign	Het
Gprc6a	A	T	10: 51,491,907 (GRCm39)	I543K	probably damaging	Het
Ifi205	T	C	1: 173,854,830 (GRCm39)	T110A	possibly damaging	Het
Med27	T	A	2: 29,399,453 (GRCm39)	S95T	probably damaging	Het
Mocs1	T	C	17: 49,757,341 (GRCm39)	S308P	probably damaging	Het
Mrpl11	C	T	19: 5,013,397 (GRCm39)	S88F	possibly damaging	Het
Nav3	A	T	10: 109,702,845 (GRCm39)	Y229*	probably null	Het
Or2f1b	A	T	6: 42,739,899 (GRCm39)	L304F	probably damaging	Het
Or2n1e	A	G	17: 38,585,772 (GRCm39)	M37V	probably benign	Het
Or52n2c	C	T	7: 104,574,599 (GRCm39)	R124H	probably benign	Het
Or52r1	C	A	7: 102,537,158 (GRCm39)	L67F	probably benign	Het
Or5g27	T	A	2: 85,409,886 (GRCm39)	M101K	possibly damaging	Het
Pear1	A	G	3: 87,663,238 (GRCm39)	I460T	probably benign	Het
Phc3	T	A	3: 30,968,678 (GRCm39)	K816N	probably damaging	Het
Phf19	T	A	2: 34,787,129 (GRCm39)	D445V	probably damaging	Het
Pkd2l2	T	C	18: 34,563,742 (GRCm39)	F486L	probably damaging	Het
Plagl1	G	T	10: 13,003,639 (GRCm39)		probably benign	Het
Prkca	A	G	11: 107,948,671 (GRCm39)	I106T	probably benign	Het
Ptpn3	C	T	4: 57,248,681 (GRCm39)	G218R	probably damaging	Het
Ptx3	A	G	3: 66,132,130 (GRCm39)	D217G	possibly damaging	Het
Rab3d	T	C	9: 21,821,815 (GRCm39)	T209A	probably benign	Het
Rfx2	A	G	17: 57,084,473 (GRCm39)	F642S	possibly damaging	Het
Rhpn2	T	A	7: 35,075,636 (GRCm39)	M271K	possibly damaging	Het
Serpinb6b	A	G	13: 33,161,977 (GRCm39)	T259A	probably damaging	Het
Speer3	G	A	5: 13,844,705 (GRCm39)	V123M	possibly damaging	Het
Svil	T	G	18: 5,106,724 (GRCm39)	V1855G	probably damaging	Het
Thbs4	A	G	13: 92,888,303 (GRCm39)	F950L	probably benign	Het
Tiparp	T	C	3: 65,460,664 (GRCm39)	V551A	probably damaging	Het
Vmn2r71	T	A	7: 85,268,482 (GRCm39)	D228E	probably benign	Het
Vps9d1	A	T	8: 123,972,410 (GRCm39)	M497K	probably damaging	Het
Wnt5b	A	G	6: 119,410,603 (GRCm39)	V241A	probably damaging	Het
Xdh	T	C	17: 74,228,342 (GRCm39)	N353S	probably damaging	Het
Zfp526	C	T	7: 24,925,757 (GRCm39)	T672M	probably damaging	Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130,254,690 (GRCm39)	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130,254,561 (GRCm39)	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130,261,449 (GRCm39)	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130,262,290 (GRCm39)	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130,261,381 (GRCm39)	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130,254,612 (GRCm39)	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130,262,328 (GRCm39)	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130,261,263 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130,261,572 (GRCm39)	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130,261,770 (GRCm39)	missense	probably damaging	1.00
R0128:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0130:Vmn2r85	UTSW	10	130,255,054 (GRCm39)	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130,258,609 (GRCm39)	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130,265,387 (GRCm39)	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130,261,155 (GRCm39)	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130,261,788 (GRCm39)	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130,261,443 (GRCm39)	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130,262,242 (GRCm39)	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R2853:Vmn2r85	UTSW	10	130,255,035 (GRCm39)	missense	probably benign	0.03
R3084:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3085:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130,254,758 (GRCm39)	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130,254,336 (GRCm39)	missense	probably damaging	1.00
R4207:Vmn2r85	UTSW	10	130,254,574 (GRCm39)	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130,261,235 (GRCm39)	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130,254,567 (GRCm39)	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130,254,990 (GRCm39)	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130,261,113 (GRCm39)	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130,257,302 (GRCm39)	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130,258,574 (GRCm39)	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130,262,343 (GRCm39)	missense	probably damaging	1.00
R6115:Vmn2r85	UTSW	10	130,258,672 (GRCm39)	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130,261,330 (GRCm39)	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130,265,281 (GRCm39)	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130,262,529 (GRCm39)	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130,261,838 (GRCm39)	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130,261,795 (GRCm39)	missense	probably benign
R6962:Vmn2r85	UTSW	10	130,261,452 (GRCm39)	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130,258,557 (GRCm39)	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130,262,376 (GRCm39)	missense	probably benign
R7424:Vmn2r85	UTSW	10	130,254,849 (GRCm39)	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130,254,852 (GRCm39)	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130,258,735 (GRCm39)	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130,254,562 (GRCm39)	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130,261,081 (GRCm39)	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130,265,364 (GRCm39)	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130,261,820 (GRCm39)	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130,254,738 (GRCm39)	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130,261,797 (GRCm39)	missense	probably damaging	0.99
R8409:Vmn2r85	UTSW	10	130,261,257 (GRCm39)	missense	probably benign	0.16
R8547:Vmn2r85	UTSW	10	130,261,311 (GRCm39)	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130,254,171 (GRCm39)	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130,261,479 (GRCm39)	missense	probably benign
R9040:Vmn2r85	UTSW	10	130,254,311 (GRCm39)	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130,254,153 (GRCm39)	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130,265,350 (GRCm39)	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130,261,534 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r85	UTSW	10	130,255,033 (GRCm39)	missense	possibly damaging	0.92
R9405:Vmn2r85	UTSW	10	130,261,215 (GRCm39)	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130,261,387 (GRCm39)	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130,254,993 (GRCm39)	missense	probably benign
R9653:Vmn2r85	UTSW	10	130,261,694 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130,261,713 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130,254,776 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGTGGTGTGCAAAAGTGAC -3'
(R):5'- CACATTTGTCCCATGGCATG -3'

Sequencing Primer
(F):5'- TTGTGGTGTGCAAAAGTGACAGTAC -3'
(R):5'- CACTTTAGATGGACTTGGATAGGAC -3'

Posted On

2017-07-14