Mutagenetix > Incidental Mutation

Incidental Mutation 'R6476:Vipr1'

516797

Institutional Source

Beutler Lab

Gene Symbol

Vipr1

Ensembl Gene

ENSMUSG00000032528

Gene Name

vasoactive intestinal peptide receptor 1

Synonyms

VIP-R1, VPAC1, VIP receptor subtype 1

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6476 (G1)

Quality Score

146.008

Status

Not validated

Chromosome

Chromosomal Location

121471782-121502020 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121498489 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 413 (V413A)

Ref Sequence

ENSEMBL: ENSMUSP00000035115 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035115]

AlphaFold

P97751

Predicted Effect

probably benign
Transcript: ENSMUST00000035115
AA Change: V413A

PolyPhen 2 Score 0.281 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000035115
Gene: ENSMUSG00000032528
AA Change: V413A

Domain	Start	End	E-Value	Type
signal peptide	1	30	N/A	INTRINSIC
HormR	59	131	7.38e-26	SMART
Pfam:7tm_2	140	386	1.4e-95	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129394

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149959

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal lethality associated with severe neonatal growth failure, enlarged cecum, intestinal hemorrhage, and enterocyte hyperproliferation in addition to disorganized islets and impaired glucose homeostasisin surviving mice. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
a	T	C	2: 154,892,699 (GRCm39)	V126A	probably benign	Het
Adamts20	T	C	15: 94,259,691 (GRCm39)	Q336R	probably benign	Het
Ankrd36	A	G	11: 5,578,753 (GRCm39)	T6A	probably benign	Het
Arhgap33	A	G	7: 30,223,837 (GRCm39)	S731P	probably damaging	Het
Arhgef10l	G	C	4: 140,338,693 (GRCm39)	P23R	probably damaging	Het
Atf7	T	C	15: 102,502,147 (GRCm39)	D3G	probably benign	Het
Caps2	A	G	10: 112,011,465 (GRCm39)	T30A	possibly damaging	Het
Ccdc15	A	T	9: 37,253,715 (GRCm39)	I191N	probably benign	Het
Cep170	A	T	1: 176,607,917 (GRCm39)	S180T	possibly damaging	Het
Chd1	G	A	17: 17,601,250 (GRCm39)		probably null	Het
Col6a3	A	T	1: 90,709,534 (GRCm39)	N1887K	unknown	Het
Csgalnact1	C	T	8: 68,913,761 (GRCm39)	S148N	probably damaging	Het
Csgalnact1	T	A	8: 68,913,762 (GRCm39)	S148C	probably damaging	Het
Dnah14	A	G	1: 181,572,333 (GRCm39)	E2888G	probably benign	Het
Dnah7b	T	A	1: 46,281,364 (GRCm39)	Y2808*	probably null	Het
Dock10	A	C	1: 80,518,959 (GRCm39)	L1254*	probably null	Het
Eml2	T	C	7: 18,930,236 (GRCm39)	V511A	probably benign	Het
Eml6	A	G	11: 29,741,971 (GRCm39)		probably null	Het
Erbin	C	A	13: 103,977,755 (GRCm39)	D601Y	probably damaging	Het
Farsa	A	G	8: 85,583,809 (GRCm39)	E49G	probably damaging	Het
Fzd7	A	G	1: 59,523,154 (GRCm39)	M346V	probably damaging	Het
Gatd3a	T	C	10: 78,003,347 (GRCm39)	N102D	probably damaging	Het
Glg1	A	C	8: 111,926,806 (GRCm39)	S170A	possibly damaging	Het
Gpx3	A	T	11: 54,798,025 (GRCm39)	I54F	probably damaging	Het
H6pd	G	A	4: 150,067,184 (GRCm39)	H401Y	probably damaging	Het
Hspb8	A	G	5: 116,560,457 (GRCm39)	S28P	probably damaging	Het
Iqub	T	A	6: 24,449,744 (GRCm39)	N707I	probably damaging	Het
Krt9	A	T	11: 100,081,640 (GRCm39)	D296E	probably damaging	Het
Lcor	C	T	19: 41,571,518 (GRCm39)	T237I	probably benign	Het
Lnx1	A	G	5: 74,768,541 (GRCm39)	V349A	possibly damaging	Het
Map3k6	A	T	4: 132,977,397 (GRCm39)	S915C	probably damaging	Het
Mecom	C	A	3: 30,034,717 (GRCm39)	A510S	possibly damaging	Het
Mettl14	A	G	3: 123,167,686 (GRCm39)	I224T	probably damaging	Het
Mme	T	C	3: 63,251,056 (GRCm39)		probably null	Het
Nbea	T	A	3: 55,912,227 (GRCm39)	T1187S	probably benign	Het
Ndor1	T	C	2: 25,138,154 (GRCm39)	T444A	possibly damaging	Het
Nhlrc1	A	G	13: 47,167,657 (GRCm39)	L200P	possibly damaging	Het
Npc1	G	T	18: 12,334,751 (GRCm39)	S667*	probably null	Het
Nscme3l	A	C	19: 5,553,253 (GRCm39)	I176S	probably damaging	Het
Or14a256	C	T	7: 86,265,218 (GRCm39)	V212I	probably benign	Het
Or1l8	T	A	2: 36,817,595 (GRCm39)	H177L	possibly damaging	Het
Or4c109	T	A	2: 88,817,721 (GRCm39)	D275V	probably benign	Het
Or51d1	T	A	7: 102,348,310 (GRCm39)	N288K	possibly damaging	Het
Or5g9	T	A	2: 85,551,928 (GRCm39)	Y60N	probably damaging	Het
Pds5a	A	C	5: 65,791,630 (GRCm39)	I773R	possibly damaging	Het
Pgr	T	A	9: 8,964,839 (GRCm39)		probably null	Het
Plscr4	A	T	9: 92,372,819 (GRCm39)	M314L	probably benign	Het
Polr2m	A	G	9: 71,390,752 (GRCm39)	V46A	probably benign	Het
Ptk2b	T	A	14: 66,424,923 (GRCm39)	M174L	possibly damaging	Het
Sec31a	A	T	5: 100,534,008 (GRCm39)	F521I	probably benign	Het
Serpinb3d	T	C	1: 107,011,071 (GRCm39)	N47S	probably benign	Het
Shld2	T	C	14: 33,989,971 (GRCm39)	S312G	probably benign	Het
Slc24a3	T	A	2: 145,448,750 (GRCm39)	D431E	probably benign	Het
Slc34a1	C	A	13: 23,996,569 (GRCm39)	H25N	probably damaging	Het
Slc9a9	T	C	9: 94,567,191 (GRCm39)	F87L	probably benign	Het
Spata31	T	A	13: 65,065,456 (GRCm39)	S54T	possibly damaging	Het
Spata6	T	C	4: 111,632,020 (GRCm39)	S144P	probably damaging	Het
Sppl2c	A	G	11: 104,077,595 (GRCm39)	N132D	probably benign	Het
Ssu2	C	T	6: 112,351,793 (GRCm39)	G311S	probably damaging	Het
Syne1	T	A	10: 5,104,531 (GRCm39)	Q6328L	possibly damaging	Het
Tie1	T	C	4: 118,330,062 (GRCm39)	T1054A	possibly damaging	Het
Tnfaip6	T	G	2: 51,942,328 (GRCm39)	D212E	probably benign	Het
Tnxb	A	G	17: 34,909,166 (GRCm39)	T1442A	probably damaging	Het
Trim35	C	T	14: 66,546,244 (GRCm39)	T337M	probably damaging	Het
Vmn1r19	C	G	6: 57,381,578 (GRCm39)	Q44E	probably damaging	Het
Zfp1002	T	C	2: 150,097,246 (GRCm39)	D61G	probably benign	Het
Zfp266	G	T	9: 20,410,577 (GRCm39)	H533Q	probably damaging	Het
Zfp689	T	C	7: 127,043,896 (GRCm39)	S245G	probably damaging	Het

Other mutations in Vipr1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01456:Vipr1	APN	9	121,494,244 (GRCm39)	missense	probably damaging	0.99
IGL01779:Vipr1	APN	9	121,493,696 (GRCm39)	missense	probably damaging	1.00
IGL01809:Vipr1	APN	9	121,490,506 (GRCm39)	missense	possibly damaging	0.70
IGL02250:Vipr1	APN	9	121,494,255 (GRCm39)	missense	probably benign	0.10
IGL02677:Vipr1	APN	9	121,489,349 (GRCm39)	splice site	probably benign
bernalillo	UTSW	9	121,493,684 (GRCm39)	missense	probably damaging	1.00
R0036:Vipr1	UTSW	9	121,490,049 (GRCm39)	missense	probably benign
R0514:Vipr1	UTSW	9	121,487,115 (GRCm39)	missense	probably damaging	1.00
R0629:Vipr1	UTSW	9	121,489,237 (GRCm39)	nonsense	probably null
R1470:Vipr1	UTSW	9	121,494,586 (GRCm39)	missense	possibly damaging	0.66
R1470:Vipr1	UTSW	9	121,494,586 (GRCm39)	missense	possibly damaging	0.66
R1766:Vipr1	UTSW	9	121,490,485 (GRCm39)	missense	possibly damaging	0.87
R1884:Vipr1	UTSW	9	121,494,930 (GRCm39)	missense	possibly damaging	0.56
R1945:Vipr1	UTSW	9	121,497,541 (GRCm39)	missense	probably damaging	1.00
R1945:Vipr1	UTSW	9	121,497,540 (GRCm39)	missense	probably damaging	1.00
R2366:Vipr1	UTSW	9	121,494,250 (GRCm39)	missense	probably benign	0.19
R4275:Vipr1	UTSW	9	121,493,684 (GRCm39)	missense	probably damaging	1.00
R4600:Vipr1	UTSW	9	121,494,202 (GRCm39)	splice site	probably null
R5012:Vipr1	UTSW	9	121,487,111 (GRCm39)	critical splice acceptor site	probably null
R6190:Vipr1	UTSW	9	121,493,719 (GRCm39)	missense	probably damaging	1.00
R6376:Vipr1	UTSW	9	121,493,640 (GRCm39)	missense	probably damaging	1.00
R6473:Vipr1	UTSW	9	121,497,621 (GRCm39)	missense	probably damaging	1.00
R6641:Vipr1	UTSW	9	121,498,631 (GRCm39)	makesense	probably null
R6752:Vipr1	UTSW	9	121,482,959 (GRCm39)	missense	probably damaging	0.99
R7189:Vipr1	UTSW	9	121,493,620 (GRCm39)	missense	probably damaging	0.97
R7371:Vipr1	UTSW	9	121,497,621 (GRCm39)	missense	probably damaging	1.00
R7419:Vipr1	UTSW	9	121,490,539 (GRCm39)	missense	probably damaging	0.97
R7647:Vipr1	UTSW	9	121,482,905 (GRCm39)	missense	possibly damaging	0.79
R8123:Vipr1	UTSW	9	121,498,518 (GRCm39)	missense	probably damaging	1.00
R8225:Vipr1	UTSW	9	121,471,915 (GRCm39)	start codon destroyed	possibly damaging	0.59
R8675:Vipr1	UTSW	9	121,493,732 (GRCm39)	missense	probably damaging	1.00
R9256:Vipr1	UTSW	9	121,490,118 (GRCm39)	missense	probably benign	0.09
R9343:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9344:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9422:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9424:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9463:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9464:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9517:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9576:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null
R9577:Vipr1	UTSW	9	121,471,993 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATCCTGAGTTGATTCTCTGGC -3'
(R):5'- TCCCTACAAAAGGCTGGAGC -3'

Sequencing Primer
(F):5'- CTCCCAGCCCTGTCCCAAG -3'
(R):5'- GTCGCTGCAGACCTCTG -3'

Posted On

2018-05-21