Mutagenetix > Incidental Mutation

Incidental Mutation 'R6528:Or10a3m'

522074

Institutional Source

Beutler Lab

Gene Symbol

Or10a3m

Ensembl Gene

ENSMUSG00000056946

Gene Name

olfactory receptor family 10 subfamily A member 3M

Synonyms

Olfr512, MOR268-3, GA_x6K02T2PBJ9-11043421-11044365

MMRRC Submission

044654-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R6528 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108312562-108313542 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 108312638 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 14 (L14P)

Ref Sequence

ENSEMBL: ENSMUSP00000147972 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074730] [ENSMUST00000209620]

AlphaFold

Q8VFZ4

Predicted Effect

probably damaging
Transcript: ENSMUST00000074730
AA Change: L26P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000074291
Gene: ENSMUSG00000056946
AA Change: L26P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	43	320	7.8e-62	PFAM
Pfam:7TM_GPCR_Srsx	47	317	2.5e-6	PFAM
Pfam:7tm_1	53	302	2.3e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000209620
AA Change: L14P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.6%

Validation Efficiency

98% (55/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam29	C	T	8: 56,325,596 (GRCm39)	R286H	possibly damaging	Het
Arhgap29	T	A	3: 121,808,351 (GRCm39)	N1176K	probably benign	Het
Cacfd1	T	G	2: 26,908,951 (GRCm39)	D97E	probably benign	Het
Ccnj	C	A	19: 40,820,529 (GRCm39)		probably null	Het
Chad	A	G	11: 94,456,450 (GRCm39)	Y176C	probably damaging	Het
Chd5	T	C	4: 152,441,133 (GRCm39)	L191P	probably damaging	Het
Cmtm1	T	C	8: 105,035,927 (GRCm39)	D190G	possibly damaging	Het
Cyp3a16	C	T	5: 145,377,241 (GRCm39)	A449T	probably damaging	Het
Eml5	T	C	12: 98,790,896 (GRCm39)	E1334G	probably benign	Het
Endou	C	T	15: 97,617,510 (GRCm39)	E147K	probably damaging	Het
Fbxo21	C	A	5: 118,138,421 (GRCm39)	H449N	probably benign	Het
Fkbp15	A	G	4: 62,250,507 (GRCm39)	I363T	probably damaging	Het
Gm12887	C	A	4: 121,472,834 (GRCm39)	G103C	probably damaging	Het
Gm14410	T	A	2: 176,885,301 (GRCm39)	H321L	probably damaging	Het
Gtf2h3	C	T	5: 124,722,360 (GRCm39)	T121I	probably benign	Het
Irgm2	C	T	11: 58,110,878 (GRCm39)	P202S	probably benign	Het
Khsrp	G	A	17: 57,330,543 (GRCm39)	T551I	probably damaging	Het
Lpin2	T	A	17: 71,551,000 (GRCm39)	I720N	probably damaging	Het
Lypd8	G	A	11: 58,275,439 (GRCm39)	G58E	probably damaging	Het
Mdn1	G	T	4: 32,713,780 (GRCm39)	L1952F	probably damaging	Het
Med13	G	A	11: 86,189,780 (GRCm39)	P1043L	probably damaging	Het
Mycbp2	T	C	14: 103,380,317 (GRCm39)	T3832A	probably damaging	Het
Nrxn3	C	T	12: 89,479,819 (GRCm39)	R654C	probably damaging	Het
Or12j3	G	T	7: 139,953,354 (GRCm39)	H56Q	possibly damaging	Het
Or13a22	A	G	7: 140,072,964 (GRCm39)	M138V	probably damaging	Het
Or8k35	T	C	2: 86,424,809 (GRCm39)	D121G	probably damaging	Het
Pcdhb6	A	G	18: 37,467,556 (GRCm39)	D159G	probably damaging	Het
Plec	T	C	15: 76,058,630 (GRCm39)	E3759G	probably damaging	Het
Plekho1	C	T	3: 95,896,633 (GRCm39)	D236N	probably damaging	Het
Pnma1	T	A	12: 84,194,197 (GRCm39)	I169F	probably benign	Het
Ppl	T	A	16: 4,905,480 (GRCm39)	H1605L	probably benign	Het
Ppp2r2b	T	A	18: 42,821,403 (GRCm39)	M252L	probably benign	Het
Pramel29	A	G	4: 143,935,381 (GRCm39)	V120A	probably damaging	Het
Prickle4	T	A	17: 48,000,258 (GRCm39)	R246*	probably null	Het
Rad50	G	A	11: 53,543,109 (GRCm39)	T1235I	probably damaging	Het
Ranbp10	T	C	8: 106,506,588 (GRCm39)	N244S	probably damaging	Het
Robo4	T	C	9: 37,315,664 (GRCm39)	S306P	possibly damaging	Het
Shox2	C	A	3: 66,888,618 (GRCm39)	R91L	probably benign	Het
Tbx5	A	G	5: 120,021,176 (GRCm39)	E394G	probably damaging	Het
Tcl1b5	A	G	12: 105,145,258 (GRCm39)	N74S	probably benign	Het
Tgif1	G	A	17: 71,153,555 (GRCm39)		probably benign	Het
Tmem128	T	A	5: 38,423,843 (GRCm39)		probably null	Het
Trio	A	G	15: 27,805,956 (GRCm39)	S511P	probably damaging	Het
Trps1	A	G	15: 50,685,823 (GRCm39)	I114T	probably benign	Het
Ttll8	G	T	15: 88,798,441 (GRCm39)	Q765K	probably benign	Het
Usp17ld	A	T	7: 102,899,962 (GRCm39)	D323E	probably damaging	Het
Vmn1r232	G	A	17: 21,134,309 (GRCm39)	T97I	probably benign	Het
Vmn2r28	T	A	7: 5,493,684 (GRCm39)	R87S	probably benign	Het
Vps26b	C	G	9: 26,921,762 (GRCm39)	E254D	probably benign	Het
Vps8	C	A	16: 21,372,875 (GRCm39)	Y113*	probably null	Het
Wwc1	C	T	11: 35,744,264 (GRCm39)	E853K	probably benign	Het
Xcr1	A	T	9: 123,685,048 (GRCm39)	I238N	probably damaging	Het
Zar1l	T	A	5: 150,430,595 (GRCm39)	E272V	probably damaging	Het
Zfp451	A	G	1: 33,816,862 (GRCm39)	Y146H	probably damaging	Het
Zfp54	G	T	17: 21,653,736 (GRCm39)	E77*	probably null	Het

Other mutations in Or10a3m

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01889:Or10a3m	APN	7	108,313,089 (GRCm39)	missense	probably benign	0.02
IGL01912:Or10a3m	APN	7	108,313,465 (GRCm39)	missense	possibly damaging	0.60
IGL02182:Or10a3m	APN	7	108,313,075 (GRCm39)	missense	probably benign	0.02
IGL02409:Or10a3m	APN	7	108,313,366 (GRCm39)	missense	probably benign	0.00
IGL02554:Or10a3m	APN	7	108,312,949 (GRCm39)	missense	possibly damaging	0.94
IGL03210:Or10a3m	APN	7	108,312,775 (GRCm39)	missense	probably damaging	1.00
IGL03373:Or10a3m	APN	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
IGL03400:Or10a3m	APN	7	108,312,733 (GRCm39)	missense	probably benign	0.28
R0092:Or10a3m	UTSW	7	108,313,031 (GRCm39)	missense	probably benign
R0741:Or10a3m	UTSW	7	108,312,811 (GRCm39)	missense	probably benign	0.00
R1515:Or10a3m	UTSW	7	108,313,148 (GRCm39)	missense	possibly damaging	0.94
R1982:Or10a3m	UTSW	7	108,312,902 (GRCm39)	missense	probably damaging	1.00
R2176:Or10a3m	UTSW	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
R3967:Or10a3m	UTSW	7	108,313,060 (GRCm39)	missense	probably benign
R4009:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4010:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4011:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R5095:Or10a3m	UTSW	7	108,313,019 (GRCm39)	missense	probably damaging	1.00
R5271:Or10a3m	UTSW	7	108,313,424 (GRCm39)	missense	probably damaging	1.00
R5864:Or10a3m	UTSW	7	108,312,671 (GRCm39)	missense	probably benign
R5926:Or10a3m	UTSW	7	108,312,794 (GRCm39)	missense	probably damaging	1.00
R6295:Or10a3m	UTSW	7	108,312,845 (GRCm39)	missense	probably damaging	0.98
R6624:Or10a3m	UTSW	7	108,312,743 (GRCm39)	missense	possibly damaging	0.50
R8029:Or10a3m	UTSW	7	108,313,037 (GRCm39)	missense	possibly damaging	0.70
R8443:Or10a3m	UTSW	7	108,313,418 (GRCm39)	missense	possibly damaging	0.79
R8737:Or10a3m	UTSW	7	108,312,964 (GRCm39)	missense	probably damaging	1.00
R9415:Or10a3m	UTSW	7	108,313,042 (GRCm39)	missense	probably damaging	1.00
R9622:Or10a3m	UTSW	7	108,312,677 (GRCm39)	missense	probably benign	0.30
X0023:Or10a3m	UTSW	7	108,313,217 (GRCm39)	missense	possibly damaging	0.79
Z1088:Or10a3m	UTSW	7	108,312,745 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CTTCAAAACTGCTAATCTGAACCTC -3'
(R):5'- GTCAGGACCACAAGCATTTTAGG -3'

Sequencing Primer
(F):5'- GAACCTCTGTTAAAAAGAGCATGTC -3'
(R):5'- TGTGCTGAAACAGAGGTCTAC -3'

Posted On

2018-06-06