Mutagenetix > Incidental Mutation

Incidental Mutation 'R6599:Vmn1r226'

525157

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r226

Ensembl Gene

ENSMUSG00000042848

Gene Name

vomeronasal 1 receptor 226

Synonyms

V1re2

MMRRC Submission

044723-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R6599 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20907770-20908666 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20908551 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 261 (N261I)

Ref Sequence

ENSEMBL: ENSMUSP00000058266 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061756]

AlphaFold

Q8R2A8

Predicted Effect

probably benign
Transcript: ENSMUST00000061756
AA Change: N261I

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000058266
Gene: ENSMUSG00000042848
AA Change: N261I

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	286	5.2e-12	PFAM
Pfam:7tm_1	22	285	9.1e-7	PFAM
Pfam:V1R	33	292	8.7e-26	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 95.9%

Validation Efficiency

94% (32/34)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	T	C	3: 137,882,250 (GRCm39)	H150R	probably benign	Het
Acbd5	C	T	2: 22,959,092 (GRCm39)		probably benign	Het
Adcyap1r1	G	A	6: 55,456,979 (GRCm39)	V237M	probably damaging	Het
Akr1c6	T	G	13: 4,499,318 (GRCm39)		probably null	Het
Ccdc7b	T	A	8: 129,893,462 (GRCm39)	F96L	probably benign	Het
Cubn	T	C	2: 13,315,484 (GRCm39)	H2983R	possibly damaging	Het
Dhx40	T	A	11: 86,695,175 (GRCm39)	I112L	possibly damaging	Het
Dnmbp	A	T	19: 43,845,025 (GRCm39)	D1070E	probably damaging	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Ep300	G	C	15: 81,470,914 (GRCm39)	D29H	unknown	Het
Exoc3	T	C	13: 74,337,277 (GRCm39)		probably null	Het
Fcsk	A	T	8: 111,619,915 (GRCm39)		probably null	Het
Gm6401	C	A	14: 41,788,821 (GRCm39)	E83*	probably null	Het
Gm8267	G	T	14: 44,955,367 (GRCm39)	T218K	possibly damaging	Het
H1f3	T	C	13: 23,739,451 (GRCm39)		probably null	Het
Hif3a	T	A	7: 16,776,530 (GRCm39)	D470V	possibly damaging	Het
Igf2r	T	C	17: 12,917,505 (GRCm39)	S1526G	possibly damaging	Het
Lrp2	T	C	2: 69,299,749 (GRCm39)	D3101G	probably damaging	Het
Megf6	A	G	4: 154,342,544 (GRCm39)		probably null	Het
Mthfs	G	A	9: 89,121,961 (GRCm39)	G149D	probably damaging	Het
Nnmt	T	C	9: 48,514,669 (GRCm39)	D116G	probably benign	Het
Nqo2	T	C	13: 34,163,539 (GRCm39)	F22S	probably damaging	Het
Or1e29	T	A	11: 73,667,506 (GRCm39)	M216L	probably benign	Het
Or7e175	T	C	9: 20,049,239 (GRCm39)	S276P	probably damaging	Het
Parm1	T	C	5: 91,741,718 (GRCm39)	S29P	possibly damaging	Het
Prokr2	C	T	2: 132,215,469 (GRCm39)	V331M	possibly damaging	Het
Ptch1	T	A	13: 63,670,918 (GRCm39)	I871F	probably damaging	Het
Rps6ka5	C	A	12: 100,564,168 (GRCm39)	G227V	probably damaging	Het
Tcaim	C	T	9: 122,663,844 (GRCm39)	Q445*	probably null	Het
Trappc14	G	T	5: 138,261,720 (GRCm39)		probably null	Het
Trpc7	T	C	13: 56,958,193 (GRCm39)		probably null	Het
Ubxn7	A	G	16: 32,203,743 (GRCm39)	E465G	probably damaging	Het
Unk	G	A	11: 115,938,628 (GRCm39)	R77Q	probably damaging	Het
Vmn1r87	T	A	7: 12,865,886 (GRCm39)	K134*	probably null	Het
Vmn2r10	T	A	5: 109,143,944 (GRCm39)	I669L	probably benign	Het
Vmn2r115	A	G	17: 23,565,006 (GRCm39)	I298V	probably benign	Het
Yipf2	T	C	9: 21,501,144 (GRCm39)	K85E	probably damaging	Het
Zfp979	A	T	4: 147,698,083 (GRCm39)	C209S	probably benign	Het

Other mutations in Vmn1r226

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Vmn1r226	APN	17	20,908,166 (GRCm39)	missense	probably damaging	1.00
IGL02103:Vmn1r226	APN	17	20,907,926 (GRCm39)	missense	probably damaging	0.98
IGL03394:Vmn1r226	APN	17	20,908,446 (GRCm39)	missense	probably benign	0.00
R0243:Vmn1r226	UTSW	17	20,907,839 (GRCm39)	missense	probably benign	0.00
R0605:Vmn1r226	UTSW	17	20,908,133 (GRCm39)	missense	probably benign	0.12
R1463:Vmn1r226	UTSW	17	20,907,994 (GRCm39)	missense	probably benign	0.00
R1571:Vmn1r226	UTSW	17	20,908,538 (GRCm39)	missense	probably damaging	0.97
R1677:Vmn1r226	UTSW	17	20,908,335 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn1r226	UTSW	17	20,907,842 (GRCm39)	missense	probably damaging	0.99
R1918:Vmn1r226	UTSW	17	20,907,842 (GRCm39)	missense	probably damaging	0.99
R1919:Vmn1r226	UTSW	17	20,907,842 (GRCm39)	missense	probably damaging	0.99
R1980:Vmn1r226	UTSW	17	20,908,308 (GRCm39)	missense	possibly damaging	0.81
R2291:Vmn1r226	UTSW	17	20,908,475 (GRCm39)	missense	probably damaging	1.00
R2377:Vmn1r226	UTSW	17	20,907,992 (GRCm39)	missense	probably benign	0.05
R3878:Vmn1r226	UTSW	17	20,908,260 (GRCm39)	missense	possibly damaging	0.79
R5166:Vmn1r226	UTSW	17	20,908,125 (GRCm39)	missense	probably benign	0.43
R6510:Vmn1r226	UTSW	17	20,908,115 (GRCm39)	missense	probably benign	0.37
R7304:Vmn1r226	UTSW	17	20,908,011 (GRCm39)	missense	probably damaging	1.00
R8836:Vmn1r226	UTSW	17	20,908,133 (GRCm39)	missense	probably benign	0.00
R8955:Vmn1r226	UTSW	17	20,908,287 (GRCm39)	missense	possibly damaging	0.79
R9387:Vmn1r226	UTSW	17	20,907,831 (GRCm39)	missense	probably damaging	1.00
X0066:Vmn1r226	UTSW	17	20,908,512 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn1r226	UTSW	17	20,908,479 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCTGCTCATTGTAAGTTCCAGTGC -3'
(R):5'- GACACACATTTTCTAAGACTCACTC -3'

Sequencing Primer
(F):5'- CATTGTAAGTTCCAGTGCTTTTATG -3'
(R):5'- GCAGAATCTGGAAATAGCG -3'

Posted On

2018-06-22