Mutagenetix > Incidental Mutation

Incidental Mutation 'R7203:Wee1'

560787

Institutional Source

Beutler Lab

Gene Symbol

Wee1

Ensembl Gene

ENSMUSG00000031016

Gene Name

WEE 1 homolog 1 (S. pombe)

Synonyms

Wee1A

MMRRC Submission

045281-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7203 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109721266-109742506 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 109734001 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 442 (V442D)

Ref Sequence

ENSEMBL: ENSMUSP00000033326 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033326]

AlphaFold

P47810

Predicted Effect

probably benign
Transcript: ENSMUST00000033326
AA Change: V442D

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000033326
Gene: ENSMUSG00000031016
AA Change: V442D

Domain	Start	End	E-Value	Type
low complexity region	62	112	N/A	INTRINSIC
Pfam:Pkinase_Tyr	298	566	1.9e-26	PFAM
Pfam:Pkinase	298	568	1.7e-61	PFAM
low complexity region	620	631	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (108/108)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930544G11Rik	T	C	6: 65,930,317 (GRCm39)	V184A	probably benign	Het
Aars2	A	G	17: 45,827,497 (GRCm39)	Y513C	probably damaging	Het
Ackr2	G	A	9: 121,738,033 (GRCm39)	C136Y	probably damaging	Het
Aldh1l1	A	G	6: 90,547,782 (GRCm39)	K414R	probably benign	Het
Arl9	A	G	5: 77,155,118 (GRCm39)	Y83C	possibly damaging	Het
Atp10a	G	T	7: 58,436,221 (GRCm39)	R337L	probably benign	Het
Atp6v1g3	A	T	1: 138,215,538 (GRCm39)	Q66L	probably damaging	Het
Atp7b	A	T	8: 22,487,351 (GRCm39)	N1321K	probably damaging	Het
Axdnd1	A	T	1: 156,209,959 (GRCm39)	M437K	probably damaging	Het
Bap1	C	A	14: 30,976,126 (GRCm39)	P147Q	probably damaging	Het
Bicd1	C	T	6: 149,414,403 (GRCm39)	T372I	possibly damaging	Het
Brix1	T	C	15: 10,483,378 (GRCm39)		probably null	Het
Btrc	A	G	19: 45,501,967 (GRCm39)		probably null	Het
C130050O18Rik	A	C	5: 139,400,129 (GRCm39)	I61L	probably benign	Het
Cfap161	A	G	7: 83,425,258 (GRCm39)	S278P	probably damaging	Het
Cgnl1	T	C	9: 71,631,815 (GRCm39)	D512G	possibly damaging	Het
Chat	T	C	14: 32,141,014 (GRCm39)	D461G	probably damaging	Het
Chl1	T	A	6: 103,668,635 (GRCm39)	V456D	probably benign	Het
Cib1	T	C	7: 79,882,120 (GRCm39)	T20A	possibly damaging	Het
Cubn	T	G	2: 13,355,814 (GRCm39)	H1806P	probably benign	Het
Dapk1	T	A	13: 60,844,149 (GRCm39)	V56E	possibly damaging	Het
Dennd4a	A	G	9: 64,803,756 (GRCm39)	N1032D	probably benign	Het
Dlg5	T	A	14: 24,188,723 (GRCm39)	E1756V	probably damaging	Het
Dnah1	T	C	14: 30,996,339 (GRCm39)	T2666A	probably benign	Het
Dnah11	A	T	12: 118,009,257 (GRCm39)	I2135N	possibly damaging	Het
Dnah6	T	A	6: 73,150,528 (GRCm39)	E745V	probably benign	Het
Dock8	A	T	19: 25,158,927 (GRCm39)	N1695I	probably damaging	Het
Esf1	C	T	2: 140,006,139 (GRCm39)	R336Q	possibly damaging	Het
Fam161a	A	G	11: 22,971,664 (GRCm39)		probably null	Het
Fam89a	T	C	8: 125,478,418 (GRCm39)	E44G	possibly damaging	Het
Fndc3b	A	T	3: 27,510,634 (GRCm39)	D829E	probably benign	Het
Fxr1	C	T	3: 34,100,689 (GRCm39)	T125I	possibly damaging	Het
Gfer	T	C	17: 24,914,836 (GRCm39)	D69G	probably damaging	Het
Gpatch2l	T	A	12: 86,335,711 (GRCm39)	S471T	probably benign	Het
Grm7	T	G	6: 111,335,530 (GRCm39)	I647S	possibly damaging	Het
Gsn	A	G	2: 35,188,807 (GRCm39)	I447V	probably benign	Het
H2al2c	C	T	Y: 2,599,234 (GRCm39)	L46F	possibly damaging	Het
Hao2	A	C	3: 98,784,598 (GRCm39)		probably null	Het
Ifitm10	T	C	7: 141,882,305 (GRCm39)	E155G	probably benign	Het
Igkv8-16	C	A	6: 70,363,794 (GRCm39)	W76L	probably benign	Het
Igsf21	A	T	4: 139,834,648 (GRCm39)	F75I	possibly damaging	Het
Ints14	T	A	9: 64,871,701 (GRCm39)	M13K	probably damaging	Het
Ipmk	A	T	10: 71,199,298 (GRCm39)	D53V	possibly damaging	Het
Iqcn	A	T	8: 71,169,597 (GRCm39)	E1229V	probably benign	Het
Itga8	A	G	2: 12,234,906 (GRCm39)	F451L	possibly damaging	Het
Jup	A	G	11: 100,272,560 (GRCm39)	F284S	probably damaging	Het
Kctd5	T	C	17: 24,292,209 (GRCm39)	D65G	probably benign	Het
Klrc1	A	T	6: 129,654,184 (GRCm39)	S148T	probably benign	Het
Kmt5b	A	G	19: 3,864,147 (GRCm39)	K404E	probably damaging	Het
Krt9	A	C	11: 100,081,617 (GRCm39)	M304R	probably damaging	Het
Krtap5-1	A	T	7: 141,850,299 (GRCm39)	S143T	unknown	Het
Kyat3	A	G	3: 142,426,162 (GRCm39)	N68D	probably damaging	Het
Kynu	A	G	2: 43,571,365 (GRCm39)	D427G	probably damaging	Het
Leo1	A	G	9: 75,353,278 (GRCm39)		probably null	Het
Loxhd1	A	G	18: 77,501,892 (GRCm39)	D1737G	probably damaging	Het
Lpo	C	A	11: 87,700,077 (GRCm39)	L521F	possibly damaging	Het
Lrrk1	A	G	7: 65,920,573 (GRCm39)	S1477P	probably damaging	Het
Lrrtm1	C	A	6: 77,220,584 (GRCm39)	L14M	probably damaging	Het
Ly75	G	A	2: 60,154,196 (GRCm39)	R1084*	probably null	Het
Mcf2l	A	G	8: 13,060,456 (GRCm39)	D764G	probably benign	Het
Mmut	A	T	17: 41,249,564 (GRCm39)	M180L	probably benign	Het
Mrgprd	A	T	7: 144,876,086 (GRCm39)	D319V	probably benign	Het
Myom3	T	C	4: 135,522,490 (GRCm39)	L897P	possibly damaging	Het
Ncapd2	A	T	6: 125,161,291 (GRCm39)	M247K	possibly damaging	Het
Nlrp2	T	C	7: 5,320,533 (GRCm39)	D868G	probably damaging	Het
Npy6r	A	G	18: 44,408,999 (GRCm39)	N140S	probably damaging	Het
Nsmce4a	T	C	7: 130,141,602 (GRCm39)	K196E	probably benign	Het
Nup88	T	C	11: 70,836,080 (GRCm39)	K532R	probably benign	Het
Or6k4	T	A	1: 173,964,680 (GRCm39)	Y123*	probably null	Het
Or8d6	T	A	9: 39,853,805 (GRCm39)	V83E	probably benign	Het
Or8g29-ps1	A	T	9: 39,200,589 (GRCm39)	I199N	unknown	Het
Pbxip1	A	T	3: 89,354,735 (GRCm39)	D418V	possibly damaging	Het
Pde2a	C	A	7: 101,159,151 (GRCm39)	R761S	possibly damaging	Het
Phf10	A	T	17: 15,166,575 (GRCm39)	C432S	probably damaging	Het
Pitpnm2	T	C	5: 124,259,522 (GRCm39)	D1271G	probably damaging	Het
Plin1	A	T	7: 79,373,192 (GRCm39)	L259Q	probably damaging	Het
Pmpca	G	C	2: 26,285,046 (GRCm39)	E424Q	possibly damaging	Het
Pou6f2	T	A	13: 18,414,379 (GRCm39)	Q132L	unknown	Het
Ppa2	A	T	3: 133,036,199 (GRCm39)	N118Y	possibly damaging	Het
Prickle2	T	C	6: 92,387,959 (GRCm39)	E537G	possibly damaging	Het
Prl3d1	A	G	13: 27,282,684 (GRCm39)	I141V	possibly damaging	Het
Prl8a1	A	T	13: 27,758,172 (GRCm39)	V179D	probably damaging	Het
Prr14l	A	G	5: 32,984,489 (GRCm39)	F1669L	probably benign	Het
Prrg4	T	A	2: 104,669,787 (GRCm39)	E110V	possibly damaging	Het
Rfx5	C	A	3: 94,866,187 (GRCm39)	H495Q	unknown	Het
Rgl2	C	T	17: 34,152,403 (GRCm39)	R367W	probably damaging	Het
Rpe65	A	G	3: 159,328,491 (GRCm39)	Y433C	probably damaging	Het
Rpl35rt	A	T	1: 156,193,657 (GRCm39)	I4F	probably damaging	Het
Rtn4rl1	C	T	11: 75,156,576 (GRCm39)	S336F	possibly damaging	Het
Scn2a	A	G	2: 65,578,663 (GRCm39)	D1446G	probably benign	Het
Sdk1	C	A	5: 142,031,931 (GRCm39)	T1002K	probably benign	Het
Slc9b2	T	C	3: 135,036,422 (GRCm39)	S409P	probably benign	Het
Sowahc	A	G	10: 59,058,100 (GRCm39)	T79A	probably benign	Het
Spata31h1	G	A	10: 82,129,248 (GRCm39)	T1254I	probably benign	Het
Stk35	T	A	2: 129,643,513 (GRCm39)	C166S	probably benign	Het
Tarbp2	A	G	15: 102,430,922 (GRCm39)	H225R	probably benign	Het
Tdrd12	T	A	7: 35,188,648 (GRCm39)	K530*	probably null	Het
Terf2ip	A	G	8: 112,744,618 (GRCm39)	I312V	probably benign	Het
Tgfb1	T	C	7: 25,391,964 (GRCm39)		probably null	Het
Thbs2	T	C	17: 14,891,720 (GRCm39)	D939G	probably damaging	Het
Ube4b	A	T	4: 149,483,067 (GRCm39)	I67K	probably benign	Het
Ubn1	G	T	16: 4,895,080 (GRCm39)	V709F	possibly damaging	Het
Ugt2b5	T	C	5: 87,276,258 (GRCm39)	K339E	possibly damaging	Het
Vax2	T	C	6: 83,714,882 (GRCm39)	S266P	probably damaging	Het
Vmn2r108	A	G	17: 20,683,038 (GRCm39)	I722T	probably benign	Het
Vmn2r95	A	G	17: 18,661,577 (GRCm39)	K441R	probably benign	Het
Wapl	C	A	14: 34,458,648 (GRCm39)	D903E	probably benign	Het
Zan	T	C	5: 137,432,358 (GRCm39)	N2313S	unknown	Het

Other mutations in Wee1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00693:Wee1	APN	7	109,734,060 (GRCm39)	splice site	probably null
IGL00981:Wee1	APN	7	109,738,876 (GRCm39)	missense	probably damaging	1.00
IGL01017:Wee1	APN	7	109,725,055 (GRCm39)	missense	possibly damaging	0.93
IGL01357:Wee1	APN	7	109,741,242 (GRCm39)	missense	probably benign	0.39
IGL01838:Wee1	APN	7	109,723,744 (GRCm39)	missense	probably benign	0.01
IGL01970:Wee1	APN	7	109,738,457 (GRCm39)	missense	probably damaging	1.00
IGL02396:Wee1	APN	7	109,741,300 (GRCm39)	missense	probably damaging	1.00
IGL02511:Wee1	APN	7	109,738,483 (GRCm39)	missense	possibly damaging	0.55
IGL02884:Wee1	APN	7	109,725,269 (GRCm39)	missense	probably benign	0.02
IGL03085:Wee1	APN	7	109,723,805 (GRCm39)	missense	probably damaging	1.00
IGL03221:Wee1	APN	7	109,726,024 (GRCm39)	missense	probably damaging	1.00
IGL03383:Wee1	APN	7	109,738,899 (GRCm39)	missense	probably damaging	1.00
R0220:Wee1	UTSW	7	109,723,733 (GRCm39)	missense	probably benign	0.10
R1934:Wee1	UTSW	7	109,721,698 (GRCm39)	missense	probably benign	0.06
R3110:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3112:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3978:Wee1	UTSW	7	109,723,762 (GRCm39)	missense	probably damaging	1.00
R4348:Wee1	UTSW	7	109,730,165 (GRCm39)	missense	probably damaging	1.00
R5434:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5435:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5436:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5449:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5566:Wee1	UTSW	7	109,725,257 (GRCm39)	nonsense	probably null
R5630:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5632:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5685:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5694:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5807:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5941:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R6044:Wee1	UTSW	7	109,738,513 (GRCm39)	missense	probably benign	0.00
R6163:Wee1	UTSW	7	109,734,858 (GRCm39)	missense	probably damaging	1.00
R6826:Wee1	UTSW	7	109,723,870 (GRCm39)	critical splice donor site	probably null
R7835:Wee1	UTSW	7	109,730,085 (GRCm39)	nonsense	probably null
R8273:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R8953:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R9077:Wee1	UTSW	7	109,725,963 (GRCm39)	missense	probably damaging	1.00
R9336:Wee1	UTSW	7	109,721,689 (GRCm39)	missense	probably damaging	1.00
R9463:Wee1	UTSW	7	109,721,917 (GRCm39)	missense	probably damaging	1.00
R9673:Wee1	UTSW	7	109,725,210 (GRCm39)	missense	probably damaging	0.98
R9748:Wee1	UTSW	7	109,721,722 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGTGTCATCGCCAGTCTC -3'
(R):5'- GGCGCAATGAGAACTAACAC -3'

Sequencing Primer
(F):5'- TCTTCATCACAGGTACTTCTACATG -3'
(R):5'- TGAGAACTAACACTCAACCTGTC -3'

Posted On

2019-06-26