Mutagenetix > Incidental Mutation

Incidental Mutation 'R3691:Gzmg'

268797

Institutional Source

Beutler Lab

Gene Symbol

Gzmg

Ensembl Gene

ENSMUSG00000040284

Gene Name

granzyme G

Synonyms

Ctla-7, Ctla7

MMRRC Submission

040686-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R3691 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

56394039-56397036 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 56395134 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000015578 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015578]

AlphaFold

P13366

Predicted Effect

probably benign
Transcript: ENSMUST00000015578

SMART Domains

Protein: ENSMUSP00000015578
Gene: ENSMUSG00000040284

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Tryp_SPc	20	241	6.39e-82	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224928

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 95.0%

Validation Efficiency

100% (34/34)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Afdn	A	G	17: 14,108,671 (GRCm39)	E1398G	probably damaging	Het
Avl9	C	T	6: 56,713,812 (GRCm39)	H357Y	probably benign	Het
Best2	A	G	8: 85,737,883 (GRCm39)	F171L	probably benign	Het
Btbd19	A	T	4: 116,977,789 (GRCm39)		probably benign	Het
Cap1	A	G	4: 122,758,419 (GRCm39)	S254P	probably damaging	Het
Cfap43	G	A	19: 47,885,512 (GRCm39)	L368F	probably benign	Het
Clasrp	C	A	7: 19,319,165 (GRCm39)		probably benign	Het
Cog3	T	C	14: 75,991,878 (GRCm39)	M1V	probably null	Het
Drosha	G	A	15: 12,834,724 (GRCm39)	R276H	unknown	Het
Efr3b	T	A	12: 4,032,059 (GRCm39)	D183V	possibly damaging	Het
Epha1	T	C	6: 42,338,064 (GRCm39)	T794A	probably damaging	Het
Fbxo17	G	A	7: 28,436,887 (GRCm39)	V281I	probably damaging	Het
Flii	T	C	11: 60,610,583 (GRCm39)	Y571C	probably benign	Het
Flywch1	G	A	17: 23,982,186 (GRCm39)	P6L	probably damaging	Het
Foxj3	T	C	4: 119,473,839 (GRCm39)		probably benign	Het
Fras1	C	T	5: 96,929,371 (GRCm39)	T3925I	probably benign	Het
Gk5	T	C	9: 96,011,149 (GRCm39)		probably null	Het
Gm12588	T	G	11: 121,796,751 (GRCm39)	Q366P	possibly damaging	Het
Gm3248	A	G	14: 5,943,068 (GRCm38)	I161T	probably damaging	Het
Lrrc69	A	T	4: 14,795,980 (GRCm39)	N22K	possibly damaging	Het
Med13l	A	G	5: 118,859,562 (GRCm39)	R250G	probably benign	Het
Mideas	C	T	12: 84,203,245 (GRCm39)	G886S	probably benign	Het
Mxi1	T	C	19: 53,358,062 (GRCm39)	L73P	probably damaging	Het
Myo9b	G	A	8: 71,786,981 (GRCm39)	R721Q	probably benign	Het
Napb	T	C	2: 148,544,977 (GRCm39)		probably null	Het
Nsun2	A	G	13: 69,760,456 (GRCm39)	N45D	probably damaging	Het
Or10ag2	T	C	2: 87,248,514 (GRCm39)	F39L	probably benign	Het
Oxct1	A	G	15: 4,076,999 (GRCm39)	M111V	probably benign	Het
Pcnx4	A	G	12: 72,620,493 (GRCm39)	D771G	probably damaging	Het
Serpina6	T	C	12: 103,620,668 (GRCm39)	D27G	probably benign	Het
Tecpr1	A	G	5: 144,146,797 (GRCm39)	F523S	probably benign	Het
Zan	A	G	5: 137,418,281 (GRCm39)	I2939T	unknown	Het
Zfc3h1	A	G	10: 115,256,595 (GRCm39)	T1522A	probably benign	Het

Other mutations in Gzmg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02064:Gzmg	APN	14	56,394,798 (GRCm39)	missense	probably benign	0.00
IGL02323:Gzmg	APN	14	56,394,729 (GRCm39)	missense	probably benign	0.01
IGL02633:Gzmg	APN	14	56,394,160 (GRCm39)	missense	probably damaging	0.99
R0456:Gzmg	UTSW	14	56,395,779 (GRCm39)	missense	probably damaging	1.00
R1368:Gzmg	UTSW	14	56,395,263 (GRCm39)	missense	probably benign	0.41
R1663:Gzmg	UTSW	14	56,394,265 (GRCm39)	missense	probably damaging	1.00
R2511:Gzmg	UTSW	14	56,395,832 (GRCm39)	missense	probably benign	0.00
R4509:Gzmg	UTSW	14	56,394,210 (GRCm39)	missense	probably damaging	1.00
R6776:Gzmg	UTSW	14	56,394,288 (GRCm39)	missense	probably damaging	1.00
R7175:Gzmg	UTSW	14	56,396,979 (GRCm39)	start codon destroyed	probably null
R7502:Gzmg	UTSW	14	56,394,284 (GRCm39)	missense	not run
R8022:Gzmg	UTSW	14	56,394,903 (GRCm39)	missense	probably benign	0.15
R8255:Gzmg	UTSW	14	56,395,753 (GRCm39)	missense	probably damaging	1.00
R8774:Gzmg	UTSW	14	56,394,193 (GRCm39)	missense	probably benign	0.01
R8774-TAIL:Gzmg	UTSW	14	56,394,193 (GRCm39)	missense	probably benign	0.01
R8949:Gzmg	UTSW	14	56,396,926 (GRCm39)	critical splice donor site	probably null
R9187:Gzmg	UTSW	14	56,394,714 (GRCm39)	missense	probably benign	0.00
R9422:Gzmg	UTSW	14	56,395,812 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTACTCTCCAGCTGGTAGGG -3'
(R):5'- CACAGCTCTTGCACTGCATC -3'

Sequencing Primer
(F):5'- TTACTCTCCAGCTGGTAGGGAAGAG -3'
(R):5'- TGCACTGCATCTTCTCTGAC -3'

Posted On

2015-02-19