Mutagenetix > Incidental Mutation

Incidental Mutation 'R4171:Ifna6'

318128

Institutional Source

Beutler Lab

Gene Symbol

Ifna6

Ensembl Gene

ENSMUSG00000101252

Gene Name

interferon alpha 6

Synonyms

Ifa6, Ifna8

MMRRC Submission

041011-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R4171 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88745653-88746222 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88746038 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 129 (V129A)

Ref Sequence

ENSEMBL: ENSMUSP00000100777 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105146]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000105146
AA Change: V129A

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000100777
Gene: ENSMUSG00000101252
AA Change: V129A

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	5.8e-71	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

100% (42/42)

MGI Phenotype

PHENOTYPE: Mice heterozygous for reporter allele are viable and fertile, and show normal production of interferon-alpha in response to viral infection. [provided by MGI curators]

Allele List at MGI

All alleles(1) : Targeted(1)

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700012B07Rik	G	T	11: 109,684,980 (GRCm39)	C172*	probably null	Het
2310022A10Rik	A	G	7: 27,265,109 (GRCm39)	N87S	probably benign	Het
Apmap	A	G	2: 150,425,987 (GRCm39)	V387A	probably benign	Het
B130024G19Rik	C	T	7: 70,038,454 (GRCm39)		noncoding transcript	Het
Cd200r1	A	G	16: 44,613,127 (GRCm39)	I244M	probably damaging	Het
Clic6	T	C	16: 92,293,949 (GRCm39)		probably benign	Het
Col11a1	A	G	3: 114,001,863 (GRCm39)	D429G	probably damaging	Het
Dsg4	T	A	18: 20,591,636 (GRCm39)	Y458*	probably null	Het
Elapor1	G	T	3: 108,368,259 (GRCm39)	Q885K	probably benign	Het
Fbxl13	A	G	5: 21,748,786 (GRCm39)	Y378H	probably benign	Het
Ifi203	A	C	1: 173,761,341 (GRCm39)		probably benign	Het
Igkv3-4	G	A	6: 70,649,324 (GRCm39)	A108T	probably damaging	Het
Itga1	G	A	13: 115,167,422 (GRCm39)	Q165*	probably null	Het
Kdm4c	T	A	4: 74,199,135 (GRCm39)	Y108N	possibly damaging	Het
Lap3	T	C	5: 45,666,833 (GRCm39)	S412P	probably benign	Het
Lrfn5	G	A	12: 61,890,168 (GRCm39)	V486I	probably damaging	Het
Mep1b	A	T	18: 21,228,163 (GRCm39)		probably null	Het
Mettl6	T	C	14: 31,215,624 (GRCm39)	N52S	probably damaging	Het
Nup54	A	G	5: 92,565,343 (GRCm39)	I458T	possibly damaging	Het
Or1e16	A	C	11: 73,286,365 (GRCm39)	L161R	probably damaging	Het
Or6c204	G	A	10: 129,022,453 (GRCm39)	A279V	probably benign	Het
Pecr	A	T	1: 72,315,428 (GRCm39)	N107K	probably damaging	Het
Pnpla6	A	G	8: 3,593,997 (GRCm39)	Q1307R	probably benign	Het
Poll	T	C	19: 45,544,492 (GRCm39)	K310E	probably damaging	Het
Rab29	A	G	1: 131,795,475 (GRCm39)	Q29R	probably benign	Het
Reg2	G	A	6: 78,383,574 (GRCm39)	G99D	probably damaging	Het
Rftn2	G	A	1: 55,253,429 (GRCm39)	S58L	probably damaging	Het
Rps6kb1	T	C	11: 86,435,405 (GRCm39)	E43G	possibly damaging	Het
Sdk2	T	C	11: 113,757,815 (GRCm39)		probably null	Het
Slc38a6	T	A	12: 73,397,326 (GRCm39)	Y323N	probably benign	Het
Sp100	T	C	1: 85,634,562 (GRCm39)	S152P	probably benign	Het
Tmc5	A	G	7: 118,248,810 (GRCm39)	Y605C	probably damaging	Het
Txndc17	T	C	11: 72,098,537 (GRCm39)	Y30H	probably damaging	Het
Vmn1r44	T	A	6: 89,870,996 (GRCm39)	F247L	probably damaging	Het
Zbtb39	C	T	10: 127,578,236 (GRCm39)	T270I	possibly damaging	Het
Zfp512b	G	A	2: 181,232,391 (GRCm39)		probably null	Het
Zwilch	T	A	9: 64,065,997 (GRCm39)	R255*	probably null	Het

Other mutations in Ifna6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03403:Ifna6	APN	4	88,745,695 (GRCm39)	missense	possibly damaging	0.69
R0336:Ifna6	UTSW	4	88,746,178 (GRCm39)	missense	probably damaging	0.97
R0521:Ifna6	UTSW	4	88,745,887 (GRCm39)	missense	probably benign	0.30
R2863:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2863:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R2865:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2865:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4534:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4534:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R4992:Ifna6	UTSW	4	88,745,777 (GRCm39)	missense	probably benign	0.00
R5667:Ifna6	UTSW	4	88,745,906 (GRCm39)	missense	probably damaging	1.00
R5671:Ifna6	UTSW	4	88,745,906 (GRCm39)	missense	probably damaging	1.00
R5677:Ifna6	UTSW	4	88,745,956 (GRCm39)	missense	probably benign	0.00
R6487:Ifna6	UTSW	4	88,745,743 (GRCm39)	missense	probably damaging	1.00
R6751:Ifna6	UTSW	4	88,745,987 (GRCm39)	missense	probably damaging	1.00
R7424:Ifna6	UTSW	4	88,746,044 (GRCm39)	missense	possibly damaging	0.53
R7711:Ifna6	UTSW	4	88,745,971 (GRCm39)	missense	probably benign	0.06
R8850:Ifna6	UTSW	4	88,746,222 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- GATTCCCCTTGGAGAAGGTG -3'
(R):5'- TGTCTCAGGACTCACTCGTC -3'

Sequencing Primer
(F):5'- ATGCCCAGCAGATCCAGGAG -3'
(R):5'- GTCCTCACTCAGTCTTGCCAG -3'

Posted On

2015-06-10