Mutagenetix > Incidental Mutation

Incidental Mutation 'R4414:Try4'

328060

Institutional Source

Beutler Lab

Gene Symbol

Try4

Ensembl Gene

ENSMUSG00000054106

Gene Name

trypsin 4

Synonyms

0910001B19Rik, Td

MMRRC Submission

041694-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4414 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41279206-41282467 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 41281905 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 164 (P164S)

Ref Sequence

ENSEMBL: ENSMUSP00000031913 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031913]

AlphaFold

Q9R0T7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000031913
AA Change: P164S

PolyPhen 2 Score 0.838 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000031913
Gene: ENSMUSG00000054106
AA Change: P164S

Domain	Start	End	E-Value	Type
low complexity region	3	16	N/A	INTRINSIC
Tryp_SPc	23	239	9.72e-105	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000193013

Meta Mutation Damage Score

0.3688

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503E14Rik	T	A	14: 44,406,690 (GRCm39)	M120L	probably benign	Het
Aco2	A	G	15: 81,773,584 (GRCm39)		probably null	Het
Acss1	A	G	2: 150,501,823 (GRCm39)	S115P	possibly damaging	Het
Ank2	A	T	3: 127,019,411 (GRCm39)		probably null	Het
AU041133	C	T	10: 81,987,316 (GRCm39)	T323M	probably damaging	Het
Bdp1	T	C	13: 100,167,369 (GRCm39)	D2215G	probably damaging	Het
Bod1l	G	A	5: 41,977,870 (GRCm39)	T1148I	probably benign	Het
Celsr1	C	T	15: 85,847,334 (GRCm39)	V1468I	probably benign	Het
Celsr1	A	G	15: 85,812,200 (GRCm39)	V2065A	probably damaging	Het
Cep89	A	G	7: 35,115,822 (GRCm39)		probably benign	Het
Cfap58	A	G	19: 47,941,480 (GRCm39)	K283E	possibly damaging	Het
Cog5	C	T	12: 31,710,853 (GRCm39)	Q78*	probably null	Het
Col20a1	C	T	2: 180,643,043 (GRCm39)	R796C	possibly damaging	Het
Dnah7b	A	T	1: 46,165,840 (GRCm39)	T502S	probably benign	Het
Dnm1l	A	G	16: 16,160,559 (GRCm39)		probably null	Het
Dse	A	T	10: 34,028,632 (GRCm39)	F819L	probably benign	Het
Eloa	G	T	4: 135,738,553 (GRCm39)	L136I	possibly damaging	Het
Eloa	T	A	4: 135,738,576 (GRCm39)	H128L	probably benign	Het
Fbxl6	T	C	15: 76,421,924 (GRCm39)	E205G	possibly damaging	Het
Golim4	T	G	3: 75,802,347 (GRCm39)	N287T	probably benign	Het
Gpx8	T	A	13: 113,179,682 (GRCm39)	K206N	possibly damaging	Het
Iqgap3	G	T	3: 88,004,293 (GRCm39)	V460F	probably benign	Het
Kcne4	A	T	1: 78,795,651 (GRCm39)	M100L	probably benign	Het
Kmt2a	A	T	9: 44,721,077 (GRCm39)		probably benign	Het
Ktn1	G	A	14: 47,962,387 (GRCm39)	W1117*	probably null	Het
Lad1	A	G	1: 135,756,484 (GRCm39)	D364G	probably benign	Het
Lmln	T	G	16: 32,930,220 (GRCm39)	I559S	probably benign	Het
Mapk4	A	T	18: 74,063,609 (GRCm39)	F538I	possibly damaging	Het
Mlxipl	A	G	5: 135,166,253 (GRCm39)		probably benign	Het
Mmrn1	T	C	6: 60,921,570 (GRCm39)	L9P	probably damaging	Het
Mnat1	G	A	12: 73,228,601 (GRCm39)	R155H	probably damaging	Het
Mtmr11	T	C	3: 96,075,207 (GRCm39)		probably benign	Het
Naaladl1	T	C	19: 6,165,581 (GRCm39)	L745P	probably damaging	Het
Obsl1	A	T	1: 75,467,546 (GRCm39)	D1409E	probably benign	Het
Oit3	T	C	10: 59,263,925 (GRCm39)	Y403C	probably damaging	Het
Pla2g4e	A	T	2: 120,013,194 (GRCm39)	H375Q	probably benign	Het
Prodh2	G	A	7: 30,205,877 (GRCm39)	V238M	probably damaging	Het
Rdh14	G	A	12: 10,441,231 (GRCm39)		probably null	Het
Rho	G	A	6: 115,912,191 (GRCm39)	V76I	probably benign	Het
Rock1	A	T	18: 10,080,514 (GRCm39)	M1010K	probably damaging	Het
Ros1	A	G	10: 52,038,800 (GRCm39)		probably null	Het
Sim1	A	T	10: 50,857,708 (GRCm39)	D486V	probably benign	Het
Spmip6	T	C	4: 41,505,574 (GRCm39)	T183A	possibly damaging	Het
Src	A	G	2: 157,306,573 (GRCm39)	D192G	probably damaging	Het
Stox1	T	C	10: 62,495,348 (GRCm39)	N975S	probably benign	Het
Tmem145	A	G	7: 25,006,554 (GRCm39)	Y54C	probably damaging	Het
Vegfc	A	T	8: 54,634,130 (GRCm39)	N270Y	probably benign	Het

Other mutations in Try4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01773:Try4	APN	6	41,281,960 (GRCm39)	missense	probably damaging	1.00
IGL02216:Try4	APN	6	41,281,965 (GRCm39)	missense	probably benign
R0537:Try4	UTSW	6	41,281,296 (GRCm39)	missense	probably benign
R0731:Try4	UTSW	6	41,281,301 (GRCm39)	missense	probably benign	0.01
R1113:Try4	UTSW	6	41,282,308 (GRCm39)	missense	possibly damaging	0.90
R1833:Try4	UTSW	6	41,280,365 (GRCm39)	missense	probably damaging	0.98
R2246:Try4	UTSW	6	41,282,406 (GRCm39)	missense	possibly damaging	0.80
R4131:Try4	UTSW	6	41,282,335 (GRCm39)	nonsense	probably null
R5457:Try4	UTSW	6	41,280,355 (GRCm39)	missense	probably damaging	1.00
R5707:Try4	UTSW	6	41,281,977 (GRCm39)	missense	possibly damaging	0.65
R6023:Try4	UTSW	6	41,280,355 (GRCm39)	missense	probably damaging	1.00
R7131:Try4	UTSW	6	41,281,337 (GRCm39)	missense	probably benign	0.03
R7783:Try4	UTSW	6	41,279,229 (GRCm39)	missense	possibly damaging	0.96
R8051:Try4	UTSW	6	41,281,996 (GRCm39)	missense	probably damaging	0.99
R9320:Try4	UTSW	6	41,282,008 (GRCm39)	critical splice donor site	probably null
R9730:Try4	UTSW	6	41,281,996 (GRCm39)	missense	probably damaging	1.00
RF007:Try4	UTSW	6	41,282,297 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATGTAAACAGAGCAGCATGTAAAC -3'
(R):5'- CCTCATACACTTGGGCATGC -3'

Sequencing Primer
(F):5'- CTGTTATCACTTCTAGGAAGGACATC -3'
(R):5'- TACACTTGGGCATGCTAACC -3'

Posted On

2015-07-07