Incidental Mutation 'R4590:Rnf115'
ID342731
Institutional Source Beutler Lab
Gene Symbol Rnf115
Ensembl Gene ENSMUSG00000028098
Gene Namering finger protein 115
Synonyms2610028E05Rik, Rabring7, Zfp364
Accession Numbers
Is this an essential gene? Possibly non essential (E-score: 0.429) question?
Stock #R4590 (G1)
Quality Score225
Status Not validated
Chromosome3
Chromosomal Location96727664-96791638 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to T at 96788573 bp
ZygosityHeterozygous
Amino Acid Change Threonine to Methionine at position 225 (T225M)
Ref Sequence ENSEMBL: ENSMUSP00000029740 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029740] [ENSMUST00000199051]
Predicted Effect probably benign
Transcript: ENSMUST00000029740
AA Change: T225M

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000029740
Gene: ENSMUSG00000028098
AA Change: T225M

DomainStartEndE-ValueType
low complexity region 2 17 N/A INTRINSIC
RING 229 269 1.14e-8 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000197286
Predicted Effect probably benign
Transcript: ENSMUST00000199051
SMART Domains Protein: ENSMUSP00000143321
Gene: ENSMUSG00000028098

DomainStartEndE-ValueType
low complexity region 2 17 N/A INTRINSIC
Pfam:zf-RING_3 18 49 4.5e-9 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000199974
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.2%
  • 20x: 95.0%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ankhd1 T A 18: 36,583,644 D201E probably damaging Het
Ano10 T A 9: 122,257,165 Q398L probably benign Het
Ap3d1 A T 10: 80,719,812 L319* probably null Het
BC003331 A G 1: 150,386,352 probably null Het
Cacna1e T C 1: 154,436,519 M1575V possibly damaging Het
Cep192 T A 18: 67,816,791 Y315* probably null Het
Cndp2 C A 18: 84,669,808 V353F probably damaging Het
Ctsq T A 13: 61,036,214 N298I probably benign Het
Dnah6 A T 6: 73,152,712 C1173S probably damaging Het
Dnah9 T C 11: 66,040,392 M1993V probably damaging Het
Dnhd1 T C 7: 105,714,030 V3933A probably damaging Het
Dnpep C A 1: 75,316,401 V76L probably damaging Het
Dsc3 A C 18: 19,989,695 C57W probably damaging Het
Dtx3 A G 10: 127,192,695 S222P probably damaging Het
Eml5 T C 12: 98,837,341 Y1009C possibly damaging Het
Fam169a A G 13: 97,097,585 I122V probably benign Het
Fgr T A 4: 132,995,053 V211E probably damaging Het
Flvcr1 T C 1: 191,012,146 T402A probably benign Het
Frmd5 C T 2: 121,765,031 probably null Het
Fut2 T C 7: 45,650,946 N134S possibly damaging Het
Gm10110 A T 14: 89,897,546 noncoding transcript Het
Gm7275 A T 16: 48,073,619 noncoding transcript Het
Gm904 T A 13: 50,645,249 C81* probably null Het
Herc1 T A 9: 66,437,664 V1913E probably damaging Het
Hnmt T C 2: 24,019,099 probably null Het
Ift172 T C 5: 31,253,955 E1643G probably damaging Het
Inpp4b T A 8: 81,741,411 M1K probably null Het
Keap1 G T 9: 21,237,609 A34D probably damaging Het
Krt25 T C 11: 99,318,028 probably benign Het
Lama2 A G 10: 26,989,414 V2916A probably benign Het
Ly9 G A 1: 171,593,875 Q603* probably null Het
Mis18bp1 A C 12: 65,158,506 N14K possibly damaging Het
Mmrn1 G T 6: 60,960,813 C265F probably damaging Het
Mrgprb5 C T 7: 48,168,061 E309K probably benign Het
Nrtn T C 17: 56,751,504 T166A probably damaging Het
Olfr150 T C 9: 39,736,850 F12L probably damaging Het
Osbpl7 T A 11: 97,056,272 S266R probably damaging Het
Pcdhb11 T C 18: 37,422,496 I293T probably damaging Het
Pes1 A G 11: 3,977,986 Y546C probably damaging Het
Pth1r A T 9: 110,722,271 W587R probably benign Het
Rasgrf2 A G 13: 92,038,281 Y147H probably damaging Het
Rbbp8 T A 18: 11,732,265 L737* probably null Het
Rcor3 A T 1: 192,125,917 F153L probably damaging Het
Rev3l G A 10: 39,806,933 C349Y probably damaging Het
Rnf157 A G 11: 116,359,272 V200A probably damaging Het
Scfd2 T C 5: 74,212,256 T653A probably benign Het
Sdccag8 T C 1: 176,948,292 Y590H probably damaging Het
Sema4d T A 13: 51,723,618 K59N probably benign Het
Serpinb7 C A 1: 107,451,833 H323Q probably damaging Het
Setx T A 2: 29,144,809 H435Q probably damaging Het
Sgk3 T C 1: 9,898,795 S466P possibly damaging Het
Sgsm2 T C 11: 74,851,132 M1011V probably damaging Het
Ssc4d G A 5: 135,964,684 P106L probably benign Het
Taf7 T C 18: 37,642,731 Q261R possibly damaging Het
Tbc1d2b T C 9: 90,270,500 K71R possibly damaging Het
Tff3 C T 17: 31,129,534 V15I probably benign Het
Tgfb3 C A 12: 86,077,815 V40L possibly damaging Het
Timm10 T A 2: 84,827,648 D2E possibly damaging Het
Ttc16 T A 2: 32,773,741 N74I probably damaging Het
Ttll1 G A 15: 83,497,345 T241I probably damaging Het
Uba6 T A 5: 86,112,744 D992V probably damaging Het
Vmn1r25 T A 6: 57,978,495 T270S probably benign Het
Vmn2r106 T C 17: 20,277,466 I504V probably damaging Het
Vmn2r87 G T 10: 130,479,145 H191N possibly damaging Het
Vnn1 C A 10: 23,899,405 F184L possibly damaging Het
Vtn A T 11: 78,502,206 I466F probably damaging Het
Zfp352 A C 4: 90,224,535 D304A probably damaging Het
Other mutations in Rnf115
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03388:Rnf115 APN 3 96788668 missense probably damaging 0.98
R0051:Rnf115 UTSW 3 96785022 missense probably damaging 1.00
R0051:Rnf115 UTSW 3 96785022 missense probably damaging 1.00
R1864:Rnf115 UTSW 3 96727837 unclassified probably benign
R3015:Rnf115 UTSW 3 96754359 missense probably damaging 1.00
R4030:Rnf115 UTSW 3 96785983 missense probably damaging 1.00
R5370:Rnf115 UTSW 3 96758020 missense probably benign 0.05
R5820:Rnf115 UTSW 3 96727848 unclassified probably benign
R5978:Rnf115 UTSW 3 96788666 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GACACCTACGTTTCTTGGGTTG -3'
(R):5'- TTTACTCTGAACCAGCCTCG -3'

Sequencing Primer
(F):5'- ACGTTTCTTGGGTTGTTAGAGACATC -3'
(R):5'- TGAACCAGCCTCGCCCAG -3'
Posted On2015-09-24