Mutagenetix > Incidental Mutation

Incidental Mutation 'R4664:Chd4'

353094

Institutional Source

Beutler Lab

Gene Symbol

Chd4

Ensembl Gene

ENSMUSG00000063870

Gene Name

chromodomain helicase DNA binding protein 4

Synonyms

D6Ertd380e, 9530019N15Rik, Mi-2beta

MMRRC Submission

041922-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4664 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

125073144-125107554 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 125078465 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 203 (M203V)

Ref Sequence

ENSEMBL: ENSMUSP00000108009 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056889] [ENSMUST00000112390] [ENSMUST00000112392] [ENSMUST00000155261]

AlphaFold

Q6PDQ2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000056889
AA Change: M196V

PolyPhen 2 Score 0.578 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000060054
Gene: ENSMUSG00000063870
AA Change: M196V

Domain	Start	End	E-Value	Type
low complexity region	29	45	N/A	INTRINSIC
low complexity region	49	69	N/A	INTRINSIC
low complexity region	80	92	N/A	INTRINSIC
low complexity region	107	144	N/A	INTRINSIC
Pfam:CHDNT	156	210	7.7e-35	PFAM
low complexity region	217	249	N/A	INTRINSIC
low complexity region	271	291	N/A	INTRINSIC
low complexity region	296	318	N/A	INTRINSIC
low complexity region	321	347	N/A	INTRINSIC
PHD	365	408	7.17e-15	SMART
RING	366	407	7.46e-1	SMART
low complexity region	424	443	N/A	INTRINSIC
PHD	444	487	4.41e-15	SMART
RING	445	486	2.63e0	SMART
CHROMO	492	572	8.11e-17	SMART
CHROMO	613	670	1.98e-11	SMART
low complexity region	675	694	N/A	INTRINSIC
DEXDc	715	927	2.73e-37	SMART
low complexity region	1044	1056	N/A	INTRINSIC
HELICc	1073	1157	7.61e-27	SMART
DUF1087	1282	1346	5.56e-33	SMART
DUF1086	1359	1516	4.05e-108	SMART
low complexity region	1526	1540	N/A	INTRINSIC
low complexity region	1560	1578	N/A	INTRINSIC
low complexity region	1590	1633	N/A	INTRINSIC
low complexity region	1635	1653	N/A	INTRINSIC
low complexity region	1661	1674	N/A	INTRINSIC
Pfam:CHDCT2	1727	1899	1.9e-98	PFAM
low complexity region	1903	1915	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112390
AA Change: M203V

PolyPhen 2 Score 0.578 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000108009
Gene: ENSMUSG00000063870
AA Change: M203V

Domain	Start	End	E-Value	Type
low complexity region	29	45	N/A	INTRINSIC
low complexity region	49	69	N/A	INTRINSIC
low complexity region	87	99	N/A	INTRINSIC
low complexity region	114	151	N/A	INTRINSIC
Pfam:CHDNT	164	217	2e-28	PFAM
low complexity region	224	256	N/A	INTRINSIC
low complexity region	278	298	N/A	INTRINSIC
low complexity region	303	325	N/A	INTRINSIC
low complexity region	328	354	N/A	INTRINSIC
PHD	372	415	7.17e-15	SMART
RING	373	414	7.46e-1	SMART
low complexity region	431	450	N/A	INTRINSIC
PHD	451	494	4.41e-15	SMART
RING	452	493	2.63e0	SMART
CHROMO	499	579	8.11e-17	SMART
CHROMO	620	677	1.98e-11	SMART
low complexity region	682	701	N/A	INTRINSIC
DEXDc	722	934	2.73e-37	SMART
low complexity region	1051	1063	N/A	INTRINSIC
HELICc	1080	1164	7.61e-27	SMART
DUF1087	1289	1353	5.56e-33	SMART
DUF1086	1366	1523	4.05e-108	SMART
low complexity region	1533	1547	N/A	INTRINSIC
low complexity region	1567	1585	N/A	INTRINSIC
low complexity region	1597	1640	N/A	INTRINSIC
low complexity region	1642	1660	N/A	INTRINSIC
low complexity region	1668	1681	N/A	INTRINSIC
Pfam:CHDCT2	1735	1906	4.3e-90	PFAM
low complexity region	1910	1922	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112392
AA Change: M196V

PolyPhen 2 Score 0.317 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000108011
Gene: ENSMUSG00000063870
AA Change: M196V

Domain	Start	End	E-Value	Type
low complexity region	29	45	N/A	INTRINSIC
low complexity region	49	69	N/A	INTRINSIC
low complexity region	80	92	N/A	INTRINSIC
low complexity region	107	144	N/A	INTRINSIC
Pfam:CHDNT	156	210	1.1e-34	PFAM
low complexity region	217	249	N/A	INTRINSIC
low complexity region	271	291	N/A	INTRINSIC
low complexity region	296	318	N/A	INTRINSIC
low complexity region	321	347	N/A	INTRINSIC
PHD	352	395	7.17e-15	SMART
RING	353	394	7.46e-1	SMART
low complexity region	411	430	N/A	INTRINSIC
PHD	431	474	4.41e-15	SMART
RING	432	473	2.63e0	SMART
CHROMO	479	559	8.11e-17	SMART
CHROMO	600	657	1.98e-11	SMART
low complexity region	662	681	N/A	INTRINSIC
DEXDc	702	914	2.73e-37	SMART
low complexity region	1031	1043	N/A	INTRINSIC
HELICc	1060	1144	7.61e-27	SMART
DUF1087	1269	1333	5.56e-33	SMART
DUF1086	1346	1503	4.05e-108	SMART
low complexity region	1513	1527	N/A	INTRINSIC
low complexity region	1547	1565	N/A	INTRINSIC
low complexity region	1577	1620	N/A	INTRINSIC
low complexity region	1622	1640	N/A	INTRINSIC
low complexity region	1648	1661	N/A	INTRINSIC
Pfam:CHDCT2	1714	1886	2.8e-98	PFAM
low complexity region	1890	1902	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000155261

SMART Domains

Protein: ENSMUSP00000122806
Gene: ENSMUSG00000063870

Domain	Start	End	E-Value	Type
Blast:DEXDc	4	25	1e-5	BLAST
low complexity region	29	45	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155932

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183346

Meta Mutation Damage Score

0.2457

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

96% (110/115)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
PHENOTYPE: Mice homozygous for a gene trapped allele exhibit embryonic lethality between E3.5 and E4.5, absent blastocoele failure of trophectoderm function and increased apoptosis in blastocysts. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano2	A	G	6: 125,840,501 (GRCm39)	I391V	probably benign	Het
Apc	T	A	18: 34,431,647 (GRCm39)	L349M	probably damaging	Het
Atmin	A	G	8: 117,684,698 (GRCm39)	D786G	probably damaging	Het
Atp8a1	T	A	5: 67,919,929 (GRCm39)	D379V	possibly damaging	Het
Aurkb	A	G	11: 68,939,435 (GRCm39)	K173E	probably damaging	Het
Bak1	T	C	17: 27,241,510 (GRCm39)	I83V	possibly damaging	Het
Btbd17	A	C	11: 114,684,832 (GRCm39)	V69G	probably damaging	Het
Cacna1a	T	A	8: 85,328,396 (GRCm39)	Y1597*	probably null	Het
Camk2a	C	A	18: 61,088,696 (GRCm39)	Q167K	possibly damaging	Het
Capn1	A	T	19: 6,061,045 (GRCm39)	N253K	probably benign	Het
Ccer2	A	G	7: 28,455,928 (GRCm39)	E40G	probably benign	Het
Cdc42bpa	A	G	1: 179,972,130 (GRCm39)	T527A	probably damaging	Het
Cdkl2	T	C	5: 92,185,124 (GRCm39)	D89G	probably damaging	Het
Cep128	G	T	12: 91,263,027 (GRCm39)	R291S	probably damaging	Het
Cert1	T	C	13: 96,735,965 (GRCm39)	V175A	probably benign	Het
Chrna4	A	G	2: 180,679,286 (GRCm39)	S54P	probably damaging	Het
Cic	TGTTGCCCTC	T	7: 24,990,099 (GRCm39)		probably benign	Het
Cntn5	T	A	9: 10,144,214 (GRCm39)	I152L	possibly damaging	Het
Cntn6	A	G	6: 104,705,245 (GRCm39)	E154G	probably benign	Het
Col16a1	A	C	4: 129,955,883 (GRCm39)		probably benign	Het
Cpvl	C	T	6: 53,908,918 (GRCm39)	E282K	probably benign	Het
Cyth2	T	C	7: 45,460,143 (GRCm39)	D183G	probably damaging	Het
Ddx47	A	T	6: 134,989,319 (GRCm39)	T48S	possibly damaging	Het
Dgkk	A	G	X: 6,794,751 (GRCm39)	D685G	probably benign	Het
Dis3l	C	T	9: 64,238,080 (GRCm39)	S29N	unknown	Het
Dlg5	T	C	14: 24,187,249 (GRCm39)	H1834R	possibly damaging	Het
Dnah10	T	C	5: 124,905,536 (GRCm39)	M4060T	possibly damaging	Het
Dock3	T	A	9: 106,870,743 (GRCm39)	N557I	possibly damaging	Het
Dync2i1	T	C	12: 116,219,831 (GRCm39)	E37G	probably damaging	Het
Eprs1	A	T	1: 185,105,273 (GRCm39)		probably benign	Het
Faim2	C	A	15: 99,422,581 (GRCm39)		probably null	Het
Faim2	T	G	15: 99,422,582 (GRCm39)	S72R	probably benign	Het
Fanca	T	C	8: 123,995,711 (GRCm39)	T1364A	probably damaging	Het
Fance	T	C	17: 28,534,636 (GRCm39)		probably benign	Het
Farsb	A	T	1: 78,420,402 (GRCm39)	H496Q	possibly damaging	Het
Fryl	C	T	5: 73,248,022 (GRCm39)	E1032K	possibly damaging	Het
Galnt14	A	T	17: 73,814,808 (GRCm39)		probably benign	Het
Gba2	A	T	4: 43,568,619 (GRCm39)		probably benign	Het
Gjb4	C	A	4: 127,245,571 (GRCm39)	K123N	probably damaging	Het
Gmcl1	G	A	6: 86,709,980 (GRCm39)	T56I	probably benign	Het
Gtf2ird1	T	A	5: 134,412,756 (GRCm39)	E55V	probably damaging	Het
Gtpbp2	T	C	17: 46,472,080 (GRCm39)	V5A	probably benign	Het
Hnrnpr	A	G	4: 136,044,486 (GRCm39)		probably benign	Het
Homer3	G	A	8: 70,742,793 (GRCm39)		probably null	Het
Hsd3b3	T	C	3: 98,649,532 (GRCm39)	S264G	probably damaging	Het
Ints8	T	C	4: 11,227,152 (GRCm39)	M574V	probably benign	Het
Kif4-ps	G	A	12: 101,115,477 (GRCm39)		noncoding transcript	Het
Klhl14	A	T	18: 21,687,765 (GRCm39)	N552K	probably benign	Het
Klhl40	A	G	9: 121,609,799 (GRCm39)	E528G	probably damaging	Het
Lactb2	T	C	1: 13,717,624 (GRCm39)	E133G	probably damaging	Het
Lratd2	A	T	15: 60,695,478 (GRCm39)	D89E	probably benign	Het
Lrrtm2	T	C	18: 35,347,310 (GRCm39)		probably null	Het
Mab21l2	T	A	3: 86,454,811 (GRCm39)	Y63F	probably benign	Het
Mbd1	T	A	18: 74,402,597 (GRCm39)	I33N	possibly damaging	Het
Mertk	G	A	2: 128,643,132 (GRCm39)	V844M	probably benign	Het
Myh11	T	C	16: 14,044,448 (GRCm39)	T652A	possibly damaging	Het
Nlrp4a	C	A	7: 26,148,943 (GRCm39)	Y183*	probably null	Het
Noa1	T	A	5: 77,447,600 (GRCm39)	T558S	probably benign	Het
Nol11	A	T	11: 107,071,826 (GRCm39)	S256T	possibly damaging	Het
Nr1d1	G	T	11: 98,662,086 (GRCm39)	R183S	possibly damaging	Het
Nrg2	G	T	18: 36,185,948 (GRCm39)	Q264K	possibly damaging	Het
Nsd3	T	A	8: 26,188,894 (GRCm39)	F1027I	probably damaging	Het
Ntrk3	T	A	7: 78,110,847 (GRCm39)	I285F	probably damaging	Het
Obox8	T	C	7: 14,066,771 (GRCm39)	N91S	possibly damaging	Het
Oga	T	C	19: 45,760,384 (GRCm39)	E258G	probably benign	Het
Orc5	C	T	5: 22,751,520 (GRCm39)	S63N	probably benign	Het
Osbpl6	C	T	2: 76,398,552 (GRCm39)	T412I	probably benign	Het
P2ry14	C	T	3: 59,022,563 (GRCm39)	C308Y	probably damaging	Het
Pacsin1	T	C	17: 27,926,038 (GRCm39)	F127L	probably damaging	Het
Pex11b	T	C	3: 96,551,151 (GRCm39)	L198P	possibly damaging	Het
Pla2g4e	A	T	2: 120,001,669 (GRCm39)	V660E	probably damaging	Het
Plxna4	C	A	6: 32,493,885 (GRCm39)	V244F	possibly damaging	Het
Polr3b	A	G	10: 84,550,233 (GRCm39)	Y981C	probably damaging	Het
Popdc2	T	A	16: 38,194,649 (GRCm39)	S357T	probably damaging	Het
Prr29	A	G	11: 106,267,159 (GRCm39)	H58R	probably damaging	Het
Pyy	T	A	11: 101,998,178 (GRCm39)	M1L	possibly damaging	Het
Rasd2	T	C	8: 75,948,556 (GRCm39)	S161P	possibly damaging	Het
Ryr3	C	T	2: 112,826,900 (GRCm39)		probably benign	Het
Sectm1a	G	A	11: 120,960,552 (GRCm39)	R88C	possibly damaging	Het
Serpinb13	C	T	1: 106,910,574 (GRCm39)	S66L	probably damaging	Het
Siglecf	A	T	7: 43,005,837 (GRCm39)	I465F	possibly damaging	Het
Sorl1	A	T	9: 41,915,347 (GRCm39)	M1294K	probably damaging	Het
Spam1	A	T	6: 24,796,661 (GRCm39)	H204L	probably benign	Het
Sspo	A	T	6: 48,450,468 (GRCm39)	N2586Y	possibly damaging	Het
Tbc1d4	A	G	14: 101,700,263 (GRCm39)		probably benign	Het
Tceanc2	A	T	4: 107,022,757 (GRCm39)	S77T	probably damaging	Het
Tedc2	A	G	17: 24,439,114 (GRCm39)		probably benign	Het
Tgm6	A	G	2: 129,979,314 (GRCm39)	D148G	probably benign	Het
Tgm6	A	T	2: 129,983,128 (GRCm39)	Q239L	probably benign	Het
Thsd7a	T	A	6: 12,337,313 (GRCm39)	T1235S	possibly damaging	Het
Thsd7a	T	A	6: 12,504,012 (GRCm39)	I381F	possibly damaging	Het
Tmc4	T	C	7: 3,674,270 (GRCm39)		probably null	Het
Tmem54	A	T	4: 129,004,704 (GRCm39)	E186D	possibly damaging	Het
Tpk1	A	T	6: 43,588,269 (GRCm39)	F32I	probably benign	Het
Trpc4ap	A	G	2: 155,514,917 (GRCm39)	I97T	probably benign	Het
Tut7	G	A	13: 59,948,413 (GRCm39)	T636I	possibly damaging	Het
Txlnb	A	G	10: 17,718,942 (GRCm39)	E591G	probably damaging	Het
Tyk2	C	T	9: 21,025,503 (GRCm39)	A741T	probably damaging	Het
Ubr4	G	A	4: 139,133,829 (GRCm39)	E742K	possibly damaging	Het
Uckl1	A	T	2: 181,216,661 (GRCm39)	S95T	possibly damaging	Het
Uevld	T	A	7: 46,587,734 (GRCm39)	D322V	probably damaging	Het
Ugt1a1	CAGAGAGAGAGAGA	CAGAGAGAGAGA	1: 88,139,706 (GRCm39)		probably benign	Het
Vmn1r238	G	T	18: 3,123,300 (GRCm39)	T38K	probably damaging	Het
Vps8	T	A	16: 21,262,938 (GRCm39)		probably null	Het
Wdr83	T	C	8: 85,806,680 (GRCm39)		probably benign	Het
Zfp619	A	G	7: 39,183,559 (GRCm39)	T51A	probably benign	Het

Other mutations in Chd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00573:Chd4	APN	6	125,086,860 (GRCm39)	missense	probably damaging	1.00
IGL00917:Chd4	APN	6	125,081,909 (GRCm39)	missense	possibly damaging	0.95
IGL01088:Chd4	APN	6	125,099,431 (GRCm39)	unclassified	probably benign
IGL02005:Chd4	APN	6	125,105,779 (GRCm39)	missense	possibly damaging	0.71
IGL02405:Chd4	APN	6	125,074,190 (GRCm39)	missense	probably benign	0.06
IGL02707:Chd4	APN	6	125,085,730 (GRCm39)	missense	probably damaging	1.00
IGL02976:Chd4	APN	6	125,098,331 (GRCm39)	missense	probably damaging	1.00
IGL03001:Chd4	APN	6	125,078,529 (GRCm39)	missense	possibly damaging	0.93
FR4304:Chd4	UTSW	6	125,099,107 (GRCm39)	unclassified	probably benign
FR4589:Chd4	UTSW	6	125,099,102 (GRCm39)	unclassified	probably benign
FR4589:Chd4	UTSW	6	125,099,096 (GRCm39)	missense	probably benign	0.02
FR4737:Chd4	UTSW	6	125,099,094 (GRCm39)	unclassified	probably benign
FR4976:Chd4	UTSW	6	125,099,094 (GRCm39)	unclassified	probably benign
R0311:Chd4	UTSW	6	125,078,628 (GRCm39)	missense	probably benign	0.15
R0414:Chd4	UTSW	6	125,084,443 (GRCm39)	missense	probably damaging	1.00
R0647:Chd4	UTSW	6	125,086,086 (GRCm39)	missense	probably damaging	1.00
R0656:Chd4	UTSW	6	125,079,930 (GRCm39)	missense	probably damaging	0.98
R1342:Chd4	UTSW	6	125,074,151 (GRCm39)	missense	probably benign	0.40
R1651:Chd4	UTSW	6	125,100,547 (GRCm39)	missense	possibly damaging	0.92
R1850:Chd4	UTSW	6	125,098,619 (GRCm39)	missense	probably damaging	1.00
R2190:Chd4	UTSW	6	125,091,260 (GRCm39)	missense	probably benign	0.18
R2192:Chd4	UTSW	6	125,082,320 (GRCm39)	missense	probably damaging	0.99
R2858:Chd4	UTSW	6	125,081,849 (GRCm39)	missense	probably damaging	0.99
R3406:Chd4	UTSW	6	125,098,970 (GRCm39)	missense	probably benign	0.09
R3431:Chd4	UTSW	6	125,097,523 (GRCm39)	splice site	probably benign
R4330:Chd4	UTSW	6	125,078,565 (GRCm39)	missense	probably benign	0.29
R4394:Chd4	UTSW	6	125,098,581 (GRCm39)	missense	probably damaging	0.99
R4538:Chd4	UTSW	6	125,097,649 (GRCm39)	missense	probably damaging	0.99
R4805:Chd4	UTSW	6	125,105,908 (GRCm39)	missense	possibly damaging	0.86
R5050:Chd4	UTSW	6	125,084,443 (GRCm39)	missense	probably damaging	1.00
R5055:Chd4	UTSW	6	125,077,949 (GRCm39)	missense	possibly damaging	0.65
R5232:Chd4	UTSW	6	125,098,273 (GRCm39)	missense	probably damaging	1.00
R5314:Chd4	UTSW	6	125,077,551 (GRCm39)	missense	probably damaging	0.96
R5343:Chd4	UTSW	6	125,097,326 (GRCm39)	missense	probably damaging	1.00
R5502:Chd4	UTSW	6	125,082,239 (GRCm39)	missense	possibly damaging	0.83
R5613:Chd4	UTSW	6	125,097,509 (GRCm39)	missense	probably damaging	0.99
R6211:Chd4	UTSW	6	125,078,248 (GRCm39)	missense	possibly damaging	0.82
R6606:Chd4	UTSW	6	125,086,389 (GRCm39)	missense	probably damaging	0.99
R6753:Chd4	UTSW	6	125,091,263 (GRCm39)	missense	probably benign	0.01
R6808:Chd4	UTSW	6	125,099,086 (GRCm39)	missense	possibly damaging	0.53
R6939:Chd4	UTSW	6	125,083,501 (GRCm39)	missense	probably damaging	0.99
R6968:Chd4	UTSW	6	125,085,281 (GRCm39)	missense	probably damaging	1.00
R6973:Chd4	UTSW	6	125,099,825 (GRCm39)	missense	possibly damaging	0.53
R6992:Chd4	UTSW	6	125,091,339 (GRCm39)	missense	probably benign	0.14
R7058:Chd4	UTSW	6	125,085,405 (GRCm39)	missense	possibly damaging	0.74
R7081:Chd4	UTSW	6	125,106,948 (GRCm39)	missense	unknown
R7253:Chd4	UTSW	6	125,083,555 (GRCm39)	splice site	probably null
R7423:Chd4	UTSW	6	125,105,822 (GRCm39)	missense	possibly damaging	0.92
R7535:Chd4	UTSW	6	125,105,836 (GRCm39)	missense	probably benign	0.32
R7566:Chd4	UTSW	6	125,078,866 (GRCm39)	missense	possibly damaging	0.86
R8053:Chd4	UTSW	6	125,105,779 (GRCm39)	nonsense	probably null
R8155:Chd4	UTSW	6	125,082,287 (GRCm39)	missense	probably benign	0.00
R8711:Chd4	UTSW	6	125,100,485 (GRCm39)	unclassified	probably benign
R8783:Chd4	UTSW	6	125,100,347 (GRCm39)	missense	possibly damaging	0.53
R9020:Chd4	UTSW	6	125,084,469 (GRCm39)	missense	probably damaging	1.00
R9093:Chd4	UTSW	6	125,090,974 (GRCm39)	missense	probably benign	0.13
R9417:Chd4	UTSW	6	125,097,688 (GRCm39)	missense	probably damaging	0.99
R9509:Chd4	UTSW	6	125,099,485 (GRCm39)	missense	possibly damaging	0.96
RF046:Chd4	UTSW	6	125,099,094 (GRCm39)	unclassified	probably benign
RF052:Chd4	UTSW	6	125,099,108 (GRCm39)	unclassified	probably benign
RF058:Chd4	UTSW	6	125,099,094 (GRCm39)	unclassified	probably benign
RF060:Chd4	UTSW	6	125,099,108 (GRCm39)	unclassified	probably benign
X0025:Chd4	UTSW	6	125,083,430 (GRCm39)	nonsense	probably null
X0027:Chd4	UTSW	6	125,079,127 (GRCm39)	missense	probably damaging	0.98
X0063:Chd4	UTSW	6	125,090,978 (GRCm39)	missense	probably damaging	1.00
Z1176:Chd4	UTSW	6	125,078,561 (GRCm39)	missense	probably benign	0.36
Z1176:Chd4	UTSW	6	125,077,823 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- ATTTGTTAGGTAAGTCAGGAGGAGC -3'
(R):5'- TCAGACTTGTCAAGCCCACC -3'

Sequencing Primer
(F):5'- AGCCTGTGCGGTGGGAAG -3'
(R):5'- AGACTCTTCATTAAATTCCACTGCAC -3'

Posted On

2015-10-08