Mutagenetix > Incidental Mutation

Incidental Mutation 'R4691:Or7e165'

354923

Institutional Source

Beutler Lab

Gene Symbol

Or7e165

Ensembl Gene

ENSMUSG00000058659

Gene Name

olfactory receptor family 7 subfamily E member 165

Synonyms

Olfr58, MOR146-7P, IG6, GA_x6K02T2PVTD-13523015-13523944, MOR146-3

MMRRC Submission

041942-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R4691 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19691579-19695360 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19694678 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 83 (I83T)

Ref Sequence

ENSEMBL: ENSMUSP00000150309 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079660] [ENSMUST00000212363] [ENSMUST00000215112]

AlphaFold

Q7TRF6

Predicted Effect

probably benign
Transcript: ENSMUST00000079660
AA Change: I83T

PolyPhen 2 Score 0.331 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000078603
Gene: ENSMUSG00000063842
AA Change: I83T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	303	8.3e-8	PFAM
Pfam:7tm_1	41	290	4.5e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212363
AA Change: I45T

PolyPhen 2 Score 0.106 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000215112
AA Change: I83T

PolyPhen 2 Score 0.331 (Sensitivity: 0.90; Specificity: 0.89)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

97% (70/72)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,384,195 (GRCm39)	R3882S	probably damaging	Het
Adamts2	G	T	11: 50,647,523 (GRCm39)	V299F	probably damaging	Het
Ankrd50	A	G	3: 38,537,159 (GRCm39)	S65P	probably benign	Het
Ap4e1	T	C	2: 126,903,791 (GRCm39)	C898R	probably benign	Het
Arel1	A	T	12: 84,977,023 (GRCm39)		probably null	Het
Bag6	T	A	17: 35,358,224 (GRCm39)	V164D	probably damaging	Het
C2cd5	A	G	6: 142,975,874 (GRCm39)	S769P	possibly damaging	Het
Cables1	C	T	18: 11,973,580 (GRCm39)	Q240*	probably null	Het
Ccnb1-ps	T	A	7: 41,755,516 (GRCm39)		noncoding transcript	Het
Ccz1	A	T	5: 143,928,380 (GRCm39)	I390N	possibly damaging	Het
Ceacam23	T	A	7: 17,642,891 (GRCm39)	S434T	possibly damaging	Het
Ces1a	T	C	8: 93,759,287 (GRCm39)	H283R	probably benign	Het
Clca3b	G	A	3: 144,544,853 (GRCm39)	T378I	probably benign	Het
Cpne2	A	T	8: 95,284,849 (GRCm39)	I342F	probably damaging	Het
Cyp2d9	A	G	15: 82,340,033 (GRCm39)	D141G	probably damaging	Het
Ddias	T	A	7: 92,508,024 (GRCm39)	K630N	probably damaging	Het
Dennd4b	A	G	3: 90,179,619 (GRCm39)	T626A	probably damaging	Het
Disc1	T	C	8: 125,875,186 (GRCm39)	V554A	possibly damaging	Het
Dnah10	A	G	5: 124,852,581 (GRCm39)	T1880A	probably damaging	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Het
Epop	C	T	11: 97,519,719 (GRCm39)	G130D	possibly damaging	Het
Erap1	T	C	13: 74,821,811 (GRCm39)	L722P	probably damaging	Het
Eya4	T	A	10: 23,015,966 (GRCm39)	T334S	probably benign	Het
Ezr	T	C	17: 7,026,961 (GRCm39)	I5V	probably benign	Het
Fam53c	A	C	18: 34,901,743 (GRCm39)	E220A	probably damaging	Het
Galnt12	T	C	4: 47,104,143 (GRCm39)	S134P	probably damaging	Het
Gcfc2	T	A	6: 81,918,408 (GRCm39)	L366*	probably null	Het
Gins4	T	A	8: 23,727,075 (GRCm39)	D6V	probably benign	Het
Grid1	A	G	14: 35,291,514 (GRCm39)	H807R	probably benign	Het
H2-T22	GTTTT	GTTT	17: 36,352,462 (GRCm39)		probably null	Het
Ighv1-66	T	C	12: 115,556,929 (GRCm39)	Y51C	probably benign	Het
Inpp4b	A	T	8: 82,849,282 (GRCm39)	Y901F	probably damaging	Het
Irf2	T	A	8: 47,299,222 (GRCm39)	S339T	probably damaging	Het
Itgae	G	T	11: 73,010,345 (GRCm39)	G612*	probably null	Het
Kdr	T	C	5: 76,105,259 (GRCm39)	K1037R	possibly damaging	Het
Mro	A	T	18: 74,006,397 (GRCm39)	M115L	probably benign	Het
Myo5a	A	G	9: 75,087,438 (GRCm39)	E1098G	probably damaging	Het
Nkx2-1	T	A	12: 56,580,350 (GRCm39)	M197L	probably benign	Het
Pank2	T	A	2: 131,138,201 (GRCm39)	F430L	possibly damaging	Het
Pcx	T	A	19: 4,669,505 (GRCm39)	V794E	probably damaging	Het
Pdgfd	C	T	9: 6,288,556 (GRCm39)	P70L	probably damaging	Het
Pla2g4e	T	C	2: 120,004,781 (GRCm39)	Y521C	probably damaging	Het
Pou5f1	T	A	17: 35,817,028 (GRCm39)	F11Y	probably damaging	Het
Prdm9	A	G	17: 15,773,640 (GRCm39)	M252T	probably benign	Het
Ptk2b	C	T	14: 66,394,518 (GRCm39)	G859S	probably benign	Het
Rad51ap1	A	G	6: 126,904,516 (GRCm39)	S123P	probably benign	Het
Robo3	T	C	9: 37,336,514 (GRCm39)	E418G	probably damaging	Het
Sos2	C	T	12: 69,663,102 (GRCm39)	R631H	probably damaging	Het
Sp140l1	C	T	1: 85,066,521 (GRCm39)		probably benign	Het
St3gal2	C	T	8: 111,684,417 (GRCm39)	T25I	probably benign	Het
Stra6l	G	A	4: 45,882,851 (GRCm39)	A521T	probably benign	Het
Syt7	T	A	19: 10,403,845 (GRCm39)	L177Q	probably damaging	Het
Tet2	T	A	3: 133,191,844 (GRCm39)	Q863H	possibly damaging	Het
Tmem232	T	C	17: 65,572,237 (GRCm39)	K585E	possibly damaging	Het
Trpc6	A	C	9: 8,652,979 (GRCm39)	E595A	probably damaging	Het
Txnl1	A	G	18: 63,804,750 (GRCm39)	V248A	possibly damaging	Het
Vmn2r72	A	T	7: 85,387,119 (GRCm39)	L815*	probably null	Het
Vmn2r81	C	T	10: 79,129,211 (GRCm39)	Q701*	probably null	Het
Vps13c	T	C	9: 67,860,217 (GRCm39)	V2811A	possibly damaging	Het
Vsig10l2	T	C	9: 35,428,158 (GRCm39)	S106G	possibly damaging	Het
Zfp354a	A	G	11: 50,961,064 (GRCm39)	E425G	probably damaging	Het
Zfp617	A	T	8: 72,686,659 (GRCm39)	T330S	probably benign	Het
Zfp663	G	T	2: 165,201,050 (GRCm39)		probably benign	Het
Zfp84	T	C	7: 29,476,505 (GRCm39)	L399P	probably damaging	Het
Zfp869	A	T	8: 70,159,513 (GRCm39)	C353*	probably null	Het
Zscan22	C	T	7: 12,640,488 (GRCm39)	A85V	probably benign	Het

Other mutations in Or7e165

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Or7e165	APN	9	19,695,245 (GRCm39)	splice site	probably null
IGL01815:Or7e165	APN	9	19,695,311 (GRCm39)	missense	probably damaging	1.00
IGL02408:Or7e165	APN	9	19,694,711 (GRCm39)	missense	probably benign	0.31
IGL02644:Or7e165	APN	9	19,695,010 (GRCm39)	missense	probably benign	0.30
IGL03243:Or7e165	APN	9	19,694,564 (GRCm39)	missense	probably damaging	0.99
R0603:Or7e165	UTSW	9	19,695,235 (GRCm39)	missense	probably damaging	1.00
R2363:Or7e165	UTSW	9	19,694,892 (GRCm39)	missense	probably benign	0.00
R2570:Or7e165	UTSW	9	19,695,305 (GRCm39)	missense	probably damaging	1.00
R3890:Or7e165	UTSW	9	19,695,011 (GRCm39)	missense	probably benign	0.03
R3892:Or7e165	UTSW	9	19,695,011 (GRCm39)	missense	probably benign	0.03
R4163:Or7e165	UTSW	9	19,695,086 (GRCm39)	missense	possibly damaging	0.69
R4610:Or7e165	UTSW	9	19,694,442 (GRCm39)	nonsense	probably null
R4707:Or7e165	UTSW	9	19,694,596 (GRCm39)	missense	probably damaging	1.00
R4825:Or7e165	UTSW	9	19,694,872 (GRCm39)	missense	possibly damaging	0.74
R4950:Or7e165	UTSW	9	19,695,027 (GRCm39)	missense	probably benign
R5185:Or7e165	UTSW	9	19,694,672 (GRCm39)	missense	probably damaging	1.00
R5202:Or7e165	UTSW	9	19,694,514 (GRCm39)	missense	possibly damaging	0.46
R5439:Or7e165	UTSW	9	19,695,161 (GRCm39)	missense	probably damaging	1.00
R5669:Or7e165	UTSW	9	19,695,053 (GRCm39)	missense	probably benign	0.02
R5672:Or7e165	UTSW	9	19,694,507 (GRCm39)	missense	possibly damaging	0.92
R6038:Or7e165	UTSW	9	19,694,858 (GRCm39)	missense	probably benign
R6038:Or7e165	UTSW	9	19,694,858 (GRCm39)	missense	probably benign
R6212:Or7e165	UTSW	9	19,694,585 (GRCm39)	missense	probably damaging	1.00
R6415:Or7e165	UTSW	9	19,695,044 (GRCm39)	missense	probably damaging	1.00
R7385:Or7e165	UTSW	9	19,694,507 (GRCm39)	missense	possibly damaging	0.92
R7669:Or7e165	UTSW	9	19,694,839 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- AACTGCAGCTCATCTTATTTGCAC -3'
(R):5'- TGCACCTGTGAATCAATGAGAC -3'

Sequencing Primer
(F):5'- GCACTATTCCTGCTCATATACCTG -3'
(R):5'- CACCTGTGAATCAATGAGACTGATAC -3'

Posted On

2015-10-21