Mutagenetix > Incidental Mutation

Incidental Mutation 'R4735:Fras1'

356447

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

041962-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4735 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

96521814-96932587 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 96736022 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 539 (D539G)

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000036019
AA Change: D539G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687
AA Change: D539G

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 133 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	A	12: 71,262,897 (GRCm39)	I1410N	possibly damaging	Het
9230106D20Rik	T	C	10: 19,536,001 (GRCm39)		noncoding transcript	Het
Acsf3	T	A	8: 123,508,218 (GRCm39)	I238N	probably damaging	Het
Ahctf1	T	C	1: 179,580,964 (GRCm39)	N1746S	probably benign	Het
Akap3	T	C	6: 126,842,601 (GRCm39)	S407P	probably damaging	Het
Ankfy1	T	A	11: 72,621,437 (GRCm39)	M241K	probably benign	Het
Ano7	C	A	1: 93,328,216 (GRCm39)	T622K	probably benign	Het
App	T	C	16: 84,900,202 (GRCm39)	T83A	probably damaging	Het
Arhgef28	T	A	13: 98,036,237 (GRCm39)	E1674V	probably damaging	Het
Asb2	C	A	12: 103,291,317 (GRCm39)	V489L	probably benign	Het
Atrn	T	C	2: 130,862,910 (GRCm39)	V1330A	probably benign	Het
Bltp1	T	A	3: 37,059,116 (GRCm39)	N3267K	possibly damaging	Het
Brca1	A	G	11: 101,383,001 (GRCm39)		probably null	Het
Bspry	G	A	4: 62,404,762 (GRCm39)	R186Q	probably damaging	Het
Ccdc168	A	G	1: 44,100,861 (GRCm39)	I79T	probably benign	Het
Cdkn2d	C	G	9: 21,202,185 (GRCm39)	V21L	probably benign	Het
Celsr1	T	A	15: 85,790,230 (GRCm39)		probably null	Het
Ckap4	A	G	10: 84,369,384 (GRCm39)	V116A	possibly damaging	Het
Crb3	A	G	17: 57,372,207 (GRCm39)	T85A	probably damaging	Het
Cyld	T	C	8: 89,456,278 (GRCm39)	S443P	probably damaging	Het
Cyp27a1	T	G	1: 74,776,366 (GRCm39)	V434G	possibly damaging	Het
Cyp2b13	A	G	7: 25,787,720 (GRCm39)	T339A	probably benign	Het
Dars1	A	T	1: 128,303,971 (GRCm39)	L252*	probably null	Het
Dcaf10	G	A	4: 45,372,769 (GRCm39)	R394Q	possibly damaging	Het
Ddx55	T	C	5: 124,704,539 (GRCm39)	F382S	probably damaging	Het
Dmtf1l	T	A	X: 125,722,217 (GRCm39)	K296M	probably damaging	Het
Dnah7b	G	A	1: 46,106,115 (GRCm39)	R33Q	unknown	Het
Dnm2	G	T	9: 21,385,883 (GRCm39)	S302I	probably damaging	Het
Dock4	T	A	12: 40,681,525 (GRCm39)	F75I	probably benign	Het
Dpp10	T	C	1: 123,326,356 (GRCm39)	N365S	probably benign	Het
Dpy19l3	G	T	7: 35,422,146 (GRCm39)	Q236K	probably benign	Het
Dsp	T	C	13: 38,380,016 (GRCm39)	S1655P	probably damaging	Het
Ebpl	A	T	14: 61,579,567 (GRCm39)	I117N	probably damaging	Het
Edc4	T	A	8: 106,613,818 (GRCm39)	V386E	probably damaging	Het
Eif2s2	T	A	2: 154,720,467 (GRCm39)		probably null	Het
Elob	A	T	17: 24,046,562 (GRCm39)		probably null	Het
Enox2	T	C	X: 48,158,554 (GRCm39)	I71V	probably damaging	Het
Fez1	A	T	9: 36,772,141 (GRCm39)	K149*	probably null	Het
Fhip2a	A	G	19: 57,359,661 (GRCm39)	E67G	probably damaging	Het
Fign	A	G	2: 63,810,782 (GRCm39)	Y163H	probably damaging	Het
Flnc	G	A	6: 29,455,812 (GRCm39)	G2048S	probably damaging	Het
Frmd4b	T	C	6: 97,436,220 (GRCm39)		probably benign	Het
Gan	C	T	8: 117,920,970 (GRCm39)	T402M	probably damaging	Het
Ganc	T	A	2: 120,267,104 (GRCm39)		silent	Het
Ggt6	C	A	11: 72,327,425 (GRCm39)	R103S	probably benign	Het
Gli2	T	C	1: 118,768,052 (GRCm39)	D725G	probably damaging	Het
Gm15446	T	A	5: 110,090,818 (GRCm39)	C357S	probably damaging	Het
Gm44501	A	G	17: 40,889,810 (GRCm39)	N108S	probably benign	Het
Gm6309	A	T	5: 146,105,054 (GRCm39)	D286E	probably damaging	Het
Gm6358	G	A	16: 88,937,848 (GRCm39)	G29E	unknown	Het
Grik5	A	G	7: 24,757,713 (GRCm39)	I422T	probably damaging	Het
Grin2c	A	G	11: 115,140,422 (GRCm39)	I1232T	possibly damaging	Het
Gsg1	C	T	6: 135,214,405 (GRCm39)	R365H	possibly damaging	Het
H2-M2	A	G	17: 37,794,135 (GRCm39)	S30P	possibly damaging	Het
Hcfc2	A	G	10: 82,547,914 (GRCm39)	D302G	probably damaging	Het
Hk2	T	C	6: 82,721,955 (GRCm39)	D128G	probably benign	Het
Hmcn2	C	A	2: 31,273,787 (GRCm39)	Q1380K	probably benign	Het
Hsp90b1	G	T	10: 86,529,819 (GRCm39)	P617T	probably damaging	Het
Htr1d	A	G	4: 136,170,197 (GRCm39)	E142G	probably benign	Het
Hydin	G	A	8: 111,282,264 (GRCm39)		probably null	Het
Il1r1	T	A	1: 40,332,455 (GRCm39)	N81K	probably benign	Het
Inpp5b	T	C	4: 124,677,760 (GRCm39)	S407P	probably damaging	Het
Itpkb	T	C	1: 180,245,780 (GRCm39)	Y766H	probably damaging	Het
Lyrm2	T	A	4: 32,801,150 (GRCm39)	I65N	probably damaging	Het
Mink1	T	A	11: 70,500,086 (GRCm39)		probably null	Het
Mkrn3	C	T	7: 62,069,452 (GRCm39)	R113H	probably damaging	Het
Msx3	G	A	7: 139,627,798 (GRCm39)	A157V	probably damaging	Het
Nrxn2	T	G	19: 6,548,484 (GRCm39)	V59G	possibly damaging	Het
Nt5c3b	T	C	11: 100,331,732 (GRCm39)	T12A	probably benign	Het
Nwd2	G	A	5: 63,965,594 (GRCm39)	R1726Q	probably benign	Het
Nynrin	A	G	14: 56,107,625 (GRCm39)	T911A	probably benign	Het
Or11i1	T	A	3: 106,728,996 (GRCm39)	Y293F	probably damaging	Het
Or4p18	T	C	2: 88,233,267 (GRCm39)	T4A	probably benign	Het
Or51aa5	A	G	7: 103,167,030 (GRCm39)	I187T	possibly damaging	Het
Or51af1	C	T	7: 103,141,267 (GRCm39)	V273M	possibly damaging	Het
Or52n20	C	T	7: 104,320,200 (GRCm39)	T97I	probably benign	Het
Or5p66	A	G	7: 107,885,520 (GRCm39)	M271T	probably benign	Het
Or6c212	A	G	10: 129,558,792 (GRCm39)	I207T	probably benign	Het
Patl1	G	T	19: 11,899,869 (GRCm39)	M220I	probably benign	Het
Pcnx2	A	T	8: 126,554,780 (GRCm39)		probably null	Het
Pigr	A	T	1: 130,774,291 (GRCm39)	T424S	probably damaging	Het
Pik3c2b	T	A	1: 132,994,787 (GRCm39)	D250E	probably benign	Het
Ppa2	G	A	3: 133,076,186 (GRCm39)	E272K	probably benign	Het
Pramel7	G	A	2: 87,321,187 (GRCm39)	Q283*	probably null	Het
Prelid3b	A	G	2: 174,307,683 (GRCm39)	I81T	probably benign	Het
Prpf38a	T	C	4: 108,436,242 (GRCm39)	I24V	possibly damaging	Het
Ptger2	A	G	14: 45,239,295 (GRCm39)	D311G	possibly damaging	Het
Rab4b	A	T	7: 26,872,191 (GRCm39)		probably benign	Het
Rad17	A	C	13: 100,755,637 (GRCm39)	D581E	probably damaging	Het
Samd11	T	C	4: 156,333,230 (GRCm39)	T333A	probably benign	Het
Scaf4	C	T	16: 90,049,320 (GRCm39)	D256N	unknown	Het
Serinc2	A	G	4: 130,157,438 (GRCm39)	F82L	probably benign	Het
Serpina3g	T	A	12: 104,205,372 (GRCm39)	V37E	probably damaging	Het
Serpinb9c	T	A	13: 33,334,254 (GRCm39)	M263L	probably benign	Het
Shbg	A	G	11: 69,508,326 (GRCm39)	I67T	possibly damaging	Het
Slc25a48	G	A	13: 56,596,887 (GRCm39)		probably null	Het
Slc25a54	T	C	3: 109,005,923 (GRCm39)	W144R	probably damaging	Het
Slc34a1	A	T	13: 55,561,397 (GRCm39)	T621S	probably benign	Het
Slc6a18	A	T	13: 73,814,554 (GRCm39)	C419S	probably benign	Het
Smc5	A	T	19: 23,220,069 (GRCm39)	D389E	probably benign	Het
Smco3	T	A	6: 136,808,636 (GRCm39)	E79D	probably damaging	Het
Sox5	A	G	6: 143,906,561 (GRCm39)	F298S	probably damaging	Het
Sphkap	A	G	1: 83,256,838 (GRCm39)	S304P	probably benign	Het
Tcea2	C	T	2: 181,328,514 (GRCm39)	T211I	probably damaging	Het
Tcf4	G	T	18: 69,697,226 (GRCm39)	S34I	possibly damaging	Het
Tlr4	C	T	4: 66,759,435 (GRCm39)	R743C	probably damaging	Het
Tmem183a	T	C	1: 134,288,620 (GRCm39)	E67G	probably damaging	Het
Tpr	C	T	1: 150,317,947 (GRCm39)	R2152C	possibly damaging	Het
Trbv15	T	C	6: 41,118,358 (GRCm39)	I38T	probably benign	Het
Treh	G	A	9: 44,592,849 (GRCm39)	A125T	probably damaging	Het
Trio	A	T	15: 27,752,875 (GRCm39)		probably null	Het
Ttn	A	G	2: 76,782,293 (GRCm39)	V981A	probably damaging	Het
Uap1l1	A	G	2: 25,252,732 (GRCm39)	L436P	probably damaging	Het
Uba7	A	T	9: 107,854,115 (GRCm39)	I182F	possibly damaging	Het
Unc13c	A	T	9: 73,600,620 (GRCm39)	S1375T	probably benign	Het
Usp48	C	CT	4: 137,360,680 (GRCm39)		probably null	Het
Utp20	A	C	10: 88,652,780 (GRCm39)	V378G	possibly damaging	Het
Vmn1r69	A	T	7: 10,314,926 (GRCm39)		probably benign	Het
Vmn1r86	A	T	7: 12,836,221 (GRCm39)	H218Q	probably damaging	Het
Vmn2r121	T	A	X: 123,038,335 (GRCm39)	I562L	probably benign	Het
Vmn2r45	A	T	7: 8,486,472 (GRCm39)	I272N	probably damaging	Het
Wdr90	A	G	17: 26,078,424 (GRCm39)	V320A	probably benign	Het
Wfikkn1	A	G	17: 26,097,367 (GRCm39)	V319A	possibly damaging	Het
Whamm	A	G	7: 81,221,122 (GRCm39)	D18G	probably benign	Het
Wrn	T	A	8: 33,775,250 (GRCm39)	I605F	probably damaging	Het
Zdhhc18	A	G	4: 133,341,178 (GRCm39)	F232L	probably damaging	Het
Zfp41	T	C	15: 75,490,609 (GRCm39)	I187T	probably benign	Het
Zfp418	A	G	7: 7,185,561 (GRCm39)	Y508C	probably damaging	Het
Zfp560	T	G	9: 20,260,347 (GRCm39)	I172L	probably benign	Het
Zfp943	A	T	17: 22,211,391 (GRCm39)	D159V	probably benign	Het
Zfp955a	T	C	17: 33,460,696 (GRCm39)	I479V	probably benign	Het
Zfpm1	T	A	8: 123,062,219 (GRCm39)	V426D	probably benign	Het
Zic1	C	T	9: 91,246,558 (GRCm39)	M171I	possibly damaging	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96,887,217 (GRCm39)	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96,926,048 (GRCm39)	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96,907,309 (GRCm39)	splice site	probably benign
IGL00772:Fras1	APN	5	96,783,971 (GRCm39)	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96,682,712 (GRCm39)	splice site	probably benign
IGL00913:Fras1	APN	5	96,842,935 (GRCm39)	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96,929,140 (GRCm39)	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96,703,080 (GRCm39)	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96,821,557 (GRCm39)	missense	probably null	0.58
IGL01307:Fras1	APN	5	96,929,551 (GRCm39)	missense	probably benign
IGL01481:Fras1	APN	5	96,805,100 (GRCm39)	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96,887,195 (GRCm39)	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96,857,750 (GRCm39)	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96,906,007 (GRCm39)	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96,925,904 (GRCm39)	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96,735,990 (GRCm39)	missense	probably benign
IGL01869:Fras1	APN	5	96,856,642 (GRCm39)	splice site	probably benign
IGL01923:Fras1	APN	5	96,883,139 (GRCm39)	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96,887,107 (GRCm39)	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96,848,382 (GRCm39)	missense	probably benign
IGL02132:Fras1	APN	5	96,929,496 (GRCm39)	nonsense	probably null
IGL02171:Fras1	APN	5	96,883,040 (GRCm39)	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96,793,730 (GRCm39)	nonsense	probably null
IGL02277:Fras1	APN	5	96,735,977 (GRCm39)	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96,805,267 (GRCm39)	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96,917,372 (GRCm39)	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96,876,475 (GRCm39)	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96,692,883 (GRCm39)	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96,892,564 (GRCm39)	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96,839,230 (GRCm39)	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96,682,725 (GRCm39)	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96,926,034 (GRCm39)	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96,850,040 (GRCm39)	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96,857,960 (GRCm39)	missense	probably benign
IGL03078:Fras1	APN	5	96,783,994 (GRCm39)	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96,912,760 (GRCm39)	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96,874,394 (GRCm39)	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96,881,640 (GRCm39)	splice site	probably benign
IGL03189:Fras1	APN	5	96,890,930 (GRCm39)	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96,925,965 (GRCm39)	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96,855,350 (GRCm39)	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96,929,619 (GRCm39)	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96,881,803 (GRCm39)	splice site	probably benign
IGL03394:Fras1	APN	5	96,815,336 (GRCm39)	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96,876,440 (GRCm39)	missense	probably damaging	0.99
baby_ruth	UTSW	5	96,856,617 (GRCm39)	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96,888,688 (GRCm39)	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96,703,160 (GRCm39)	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96,825,175 (GRCm39)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,924,481 (GRCm39)	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96,924,481 (GRCm39)	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96,762,776 (GRCm39)	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96,857,936 (GRCm39)	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96,924,493 (GRCm39)	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96,884,868 (GRCm39)	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96,744,747 (GRCm39)	missense	probably benign
R0352:Fras1	UTSW	5	96,874,399 (GRCm39)	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96,910,449 (GRCm39)	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96,703,190 (GRCm39)	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96,903,368 (GRCm39)	nonsense	probably null
R0395:Fras1	UTSW	5	96,917,512 (GRCm39)	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96,839,231 (GRCm39)	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96,910,524 (GRCm39)	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96,862,202 (GRCm39)	splice site	probably null
R0456:Fras1	UTSW	5	96,702,647 (GRCm39)	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96,784,662 (GRCm39)	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96,848,347 (GRCm39)	splice site	probably benign
R0652:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96,815,246 (GRCm39)	splice site	probably benign
R0765:Fras1	UTSW	5	96,700,655 (GRCm39)	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96,916,289 (GRCm39)	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96,900,857 (GRCm39)	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96,900,857 (GRCm39)	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96,874,402 (GRCm39)	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96,862,322 (GRCm39)	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96,856,530 (GRCm39)	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96,790,488 (GRCm39)	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96,855,167 (GRCm39)	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96,855,167 (GRCm39)	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96,748,592 (GRCm39)	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96,676,445 (GRCm39)	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96,891,046 (GRCm39)	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96,784,678 (GRCm39)	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96,861,855 (GRCm39)	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96,890,921 (GRCm39)	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96,857,837 (GRCm39)	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96,861,849 (GRCm39)	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96,848,445 (GRCm39)	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96,874,472 (GRCm39)	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96,874,472 (GRCm39)	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96,746,768 (GRCm39)	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96,746,768 (GRCm39)	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96,793,732 (GRCm39)	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96,748,643 (GRCm39)	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96,700,584 (GRCm39)	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96,702,748 (GRCm39)	splice site	probably null
R1800:Fras1	UTSW	5	96,857,741 (GRCm39)	missense	probably benign
R1806:Fras1	UTSW	5	96,912,835 (GRCm39)	missense	possibly damaging	0.88
R1806:Fras1	UTSW	5	96,861,829 (GRCm39)	splice site	probably benign
R1822:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96,918,547 (GRCm39)	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96,897,282 (GRCm39)	splice site	probably null
R1929:Fras1	UTSW	5	96,815,296 (GRCm39)	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96,860,242 (GRCm39)	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96,929,062 (GRCm39)	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96,802,164 (GRCm39)	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96,848,127 (GRCm39)	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96,881,774 (GRCm39)	missense	probably benign
R2914:Fras1	UTSW	5	96,881,774 (GRCm39)	missense	probably benign
R3054:Fras1	UTSW	5	96,912,802 (GRCm39)	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96,919,571 (GRCm39)	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96,919,571 (GRCm39)	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96,929,371 (GRCm39)	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96,793,829 (GRCm39)	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96,793,829 (GRCm39)	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96,881,791 (GRCm39)	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96,825,244 (GRCm39)	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96,918,542 (GRCm39)	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96,918,542 (GRCm39)	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96,891,017 (GRCm39)	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96,918,512 (GRCm39)	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96,918,512 (GRCm39)	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96,924,594 (GRCm39)	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96,862,235 (GRCm39)	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96,762,763 (GRCm39)	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96,848,101 (GRCm39)	missense	probably benign
R4401:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96,790,479 (GRCm39)	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96,929,207 (GRCm39)	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96,857,754 (GRCm39)	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96,929,148 (GRCm39)	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96,924,593 (GRCm39)	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96,925,947 (GRCm39)	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96,848,427 (GRCm39)	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96,883,097 (GRCm39)	missense	probably damaging	0.96
R4756:Fras1	UTSW	5	96,929,518 (GRCm39)	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96,879,477 (GRCm39)	missense	probably benign
R4820:Fras1	UTSW	5	96,876,512 (GRCm39)	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96,692,851 (GRCm39)	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96,926,018 (GRCm39)	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96,856,617 (GRCm39)	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96,784,699 (GRCm39)	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96,924,583 (GRCm39)	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96,795,357 (GRCm39)	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96,874,439 (GRCm39)	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96,798,541 (GRCm39)	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96,792,969 (GRCm39)	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96,856,616 (GRCm39)	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96,784,032 (GRCm39)	nonsense	probably null
R5214:Fras1	UTSW	5	96,917,452 (GRCm39)	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96,916,222 (GRCm39)	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96,748,609 (GRCm39)	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96,805,109 (GRCm39)	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96,888,884 (GRCm39)	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96,883,046 (GRCm39)	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96,805,125 (GRCm39)	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96,793,019 (GRCm39)	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96,927,912 (GRCm39)	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96,676,394 (GRCm39)	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96,825,236 (GRCm39)	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96,884,880 (GRCm39)	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96,876,394 (GRCm39)	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96,929,203 (GRCm39)	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96,929,596 (GRCm39)	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96,916,240 (GRCm39)	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96,857,775 (GRCm39)	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96,879,548 (GRCm39)	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96,793,807 (GRCm39)	missense	probably benign
R6052:Fras1	UTSW	5	96,912,725 (GRCm39)	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96,857,844 (GRCm39)	missense	probably benign
R6256:Fras1	UTSW	5	96,881,702 (GRCm39)	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96,912,805 (GRCm39)	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96,907,423 (GRCm39)	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96,905,953 (GRCm39)	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96,883,061 (GRCm39)	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96,929,199 (GRCm39)	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96,746,800 (GRCm39)	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96,746,800 (GRCm39)	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96,874,832 (GRCm39)	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96,876,410 (GRCm39)	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96,691,165 (GRCm39)	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96,830,237 (GRCm39)	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96,916,211 (GRCm39)	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96,762,732 (GRCm39)	nonsense	probably null
R7023:Fras1	UTSW	5	96,857,943 (GRCm39)	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96,856,535 (GRCm39)	missense	probably benign
R7102:Fras1	UTSW	5	96,718,900 (GRCm39)	missense	probably benign
R7120:Fras1	UTSW	5	96,900,819 (GRCm39)	nonsense	probably null
R7124:Fras1	UTSW	5	96,862,260 (GRCm39)	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96,929,143 (GRCm39)	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96,925,937 (GRCm39)	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96,903,436 (GRCm39)	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96,784,635 (GRCm39)	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96,762,771 (GRCm39)	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96,887,173 (GRCm39)	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96,784,668 (GRCm39)	missense	probably benign	0.00
R7222:Fras1	UTSW	5	96,784,045 (GRCm39)	missense	probably damaging	1.00
R7320:Fras1	UTSW	5	96,857,745 (GRCm39)	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96,884,829 (GRCm39)	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96,744,644 (GRCm39)	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96,860,309 (GRCm39)	nonsense	probably null
R7412:Fras1	UTSW	5	96,762,748 (GRCm39)	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96,821,458 (GRCm39)	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96,916,297 (GRCm39)	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96,888,713 (GRCm39)	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96,691,173 (GRCm39)	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96,832,296 (GRCm39)	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96,832,295 (GRCm39)	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96,840,483 (GRCm39)	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96,792,962 (GRCm39)	nonsense	probably null
R7722:Fras1	UTSW	5	96,917,413 (GRCm39)	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96,860,310 (GRCm39)	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96,874,754 (GRCm39)	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96,900,763 (GRCm39)	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96,887,177 (GRCm39)	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96,929,443 (GRCm39)	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96,910,536 (GRCm39)	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96,892,633 (GRCm39)	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96,842,778 (GRCm39)	missense	probably benign
R8117:Fras1	UTSW	5	96,855,245 (GRCm39)	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96,702,714 (GRCm39)	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96,736,050 (GRCm39)	missense	probably benign
R8315:Fras1	UTSW	5	96,891,041 (GRCm39)	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96,744,711 (GRCm39)	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96,857,825 (GRCm39)	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96,839,260 (GRCm39)	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96,903,236 (GRCm39)	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96,929,207 (GRCm39)	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96,917,465 (GRCm39)	missense
R8871:Fras1	UTSW	5	96,855,257 (GRCm39)	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96,907,402 (GRCm39)	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96,929,138 (GRCm39)	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96,715,855 (GRCm39)	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96,900,774 (GRCm39)	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96,917,366 (GRCm39)	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96,860,415 (GRCm39)	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96,783,923 (GRCm39)	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96,888,609 (GRCm39)	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96,874,742 (GRCm39)	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96,883,022 (GRCm39)	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96,805,087 (GRCm39)	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96,692,904 (GRCm39)	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96,832,220 (GRCm39)	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96,910,359 (GRCm39)	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96,682,751 (GRCm39)	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96,884,813 (GRCm39)	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96,924,557 (GRCm39)	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96,929,251 (GRCm39)	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96,900,873 (GRCm39)	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96,887,124 (GRCm39)	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96,927,929 (GRCm39)	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96,884,895 (GRCm39)	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96,888,551 (GRCm39)	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96,910,387 (GRCm39)	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96,929,545 (GRCm39)	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96,717,353 (GRCm39)	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96,891,070 (GRCm39)	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1176:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1177:Fras1	UTSW	5	96,848,110 (GRCm39)	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96,839,316 (GRCm39)	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96,906,001 (GRCm39)	missense	probably benign
Z1177:Fras1	UTSW	5	96,888,842 (GRCm39)	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96,888,670 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AAACCTAGCATTTCAGGAGCC -3'
(R):5'- TGCCTAAGGTGAAGTGAATGTCAG -3'

Sequencing Primer
(F):5'- GGAGCCCTGGAACCAGCTTATATC -3'
(R):5'- TGAATGTCAGAAGTCCTGCAC -3'

Posted On

2015-11-11