Incidental Mutation 'R5524:Zfp28'
ID 431745
Institutional Source Beutler Lab
Gene Symbol Zfp28
Ensembl Gene ENSMUSG00000062861
Gene Name zinc finger protein 28
Synonyms 2810438M17Rik, mkr-5, Zfp-28
MMRRC Submission 043082-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.138) question?
Stock # R5524 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 6386317-6399636 bp(+) (GRCm39)
Type of Mutation splice site (1262 bp from exon)
DNA Base Change (assembly) A to T at 6397850 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Gene Model predicted gene model for transcript(s): [ENSMUST00000081022] [ENSMUST00000161855]
AlphaFold P10078
Predicted Effect probably null
Transcript: ENSMUST00000081022
AA Change: R762*
SMART Domains Protein: ENSMUSP00000079812
Gene: ENSMUSG00000062861
AA Change: R762*

DomainStartEndE-ValueType
low complexity region 27 50 N/A INTRINSIC
KRAB 103 163 3.53e-33 SMART
ZnF_C2H2 377 399 3.95e-4 SMART
ZnF_C2H2 405 427 6.88e-4 SMART
ZnF_C2H2 433 456 1.2e-3 SMART
ZnF_C2H2 462 484 9.58e-3 SMART
ZnF_C2H2 490 512 2.57e-3 SMART
ZnF_C2H2 518 540 1.82e-3 SMART
ZnF_C2H2 546 568 2.4e-3 SMART
ZnF_C2H2 574 596 6.32e-3 SMART
ZnF_C2H2 602 624 1.38e-3 SMART
ZnF_C2H2 630 652 4.87e-4 SMART
ZnF_C2H2 658 680 2.91e-2 SMART
ZnF_C2H2 686 708 2.36e-2 SMART
ZnF_C2H2 714 736 6.42e-4 SMART
ZnF_C2H2 742 764 2.4e-3 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159318
Predicted Effect noncoding transcript
Transcript: ENSMUST00000160568
Predicted Effect probably benign
Transcript: ENSMUST00000161855
SMART Domains Protein: ENSMUSP00000130202
Gene: ENSMUSG00000093536

DomainStartEndE-ValueType
low complexity region 57 72 N/A INTRINSIC
transmembrane domain 85 107 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000207086
Predicted Effect probably null
Transcript: ENSMUST00000207465
Predicted Effect probably null
Transcript: ENSMUST00000207809
Predicted Effect probably benign
Transcript: ENSMUST00000208949
Predicted Effect probably null
Transcript: ENSMUST00000208338
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 98.3%
  • 3x: 97.3%
  • 10x: 95.4%
  • 20x: 91.4%
Validation Efficiency 100% (76/76)
Allele List at MGI
Other mutations in this stock
Total: 70 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700010L04Rik T A 7: 82,503,150 (GRCm39) noncoding transcript Het
6030469F06Rik A G 12: 31,234,862 (GRCm39) noncoding transcript Het
A830031A19Rik T A 11: 24,008,776 (GRCm39) I13F unknown Het
Acsbg2 A C 17: 57,157,197 (GRCm39) L309R probably damaging Het
Adam32 A T 8: 25,412,328 (GRCm39) M76K probably damaging Het
Adamts14 C A 10: 61,066,222 (GRCm39) R297L probably damaging Het
Agbl5 G A 5: 31,051,247 (GRCm39) probably null Het
Asb14 A G 14: 26,622,408 (GRCm39) K80E possibly damaging Het
Baiap2l1 T C 5: 144,217,759 (GRCm39) T276A probably benign Het
Cacna1d G A 14: 29,764,086 (GRCm39) P2127S probably benign Het
Ces2g A G 8: 105,693,527 (GRCm39) T403A probably benign Het
Cgn A C 3: 94,687,299 (GRCm39) M1R probably null Het
Chd5 T G 4: 152,461,087 (GRCm39) S1226A probably benign Het
Col18a1 A G 10: 76,894,558 (GRCm39) V1497A probably damaging Het
Cpd A T 11: 76,688,727 (GRCm39) Y848* probably null Het
Cyp2a12 T C 7: 26,730,656 (GRCm39) V207A probably benign Het
Cyth1 G A 11: 118,073,593 (GRCm39) R247W probably benign Het
Derl3 T C 10: 75,730,324 (GRCm39) V129A possibly damaging Het
Ehmt2 C T 17: 35,118,067 (GRCm39) R40* probably null Het
Eml1 A T 12: 108,487,635 (GRCm39) I518L probably damaging Het
Eri1 A G 8: 35,945,763 (GRCm39) V174A probably benign Het
Fgd3 T A 13: 49,431,053 (GRCm39) I435F probably damaging Het
Foxd1 T G 13: 98,492,412 (GRCm39) S429A unknown Het
Ftcd T A 10: 76,425,165 (GRCm39) probably benign Het
Gm5478 G T 15: 101,553,102 (GRCm39) N323K probably benign Het
Gnpda1 G T 18: 38,468,161 (GRCm39) P45Q probably damaging Het
Kif20a G T 18: 34,763,678 (GRCm39) probably null Het
Klrb1 T C 6: 128,689,296 (GRCm39) probably null Het
Lcorl C A 5: 45,932,864 (GRCm39) probably null Het
Lcorl T A 5: 45,932,865 (GRCm39) probably null Het
Lrp1b T A 2: 41,000,900 (GRCm39) K2108M probably damaging Het
Lyst C T 13: 13,921,364 (GRCm39) P3437S probably benign Het
Macrod2 A T 2: 142,159,863 (GRCm39) M349L possibly damaging Het
Marchf7 T C 2: 60,075,647 (GRCm39) probably benign Het
Mcm9 A T 10: 53,424,786 (GRCm39) C601* probably null Het
Muc19 G T 15: 91,778,587 (GRCm39) noncoding transcript Het
Mycbp2 T C 14: 103,532,673 (GRCm39) D427G probably damaging Het
Nap1l5 C A 6: 58,883,763 (GRCm39) V64L possibly damaging Het
Ncam2 C T 16: 81,231,766 (GRCm39) R77* probably null Het
Npas3 T C 12: 54,115,721 (GRCm39) V863A possibly damaging Het
Nr2c2 T A 6: 92,116,746 (GRCm39) probably null Het
Oosp3 T C 19: 11,682,794 (GRCm39) F56S possibly damaging Het
Or1a1b A T 11: 74,097,409 (GRCm39) L211Q probably damaging Het
Or5k17 C T 16: 58,746,172 (GRCm39) C254Y probably benign Het
Or8g20 C T 9: 39,396,283 (GRCm39) V89M probably damaging Het
Plgrkt G A 19: 29,327,850 (GRCm39) P78S probably damaging Het
Prss36 G A 7: 127,533,637 (GRCm39) Q56* probably null Het
Ptprz1 A G 6: 22,986,317 (GRCm39) probably null Het
Qsox2 A T 2: 26,107,699 (GRCm39) F265I probably damaging Het
R3hcc1l G T 19: 42,552,307 (GRCm39) E435* probably null Het
Shbg T C 11: 69,507,588 (GRCm39) D163G probably benign Het
Skint7 T A 4: 111,837,546 (GRCm39) L108H probably damaging Het
Smtnl1 T A 2: 84,649,238 (GRCm39) E5D probably benign Het
Spock1 C T 13: 57,704,608 (GRCm39) G120D probably damaging Het
Stk11ip G T 1: 75,508,971 (GRCm39) C700F probably damaging Het
Sult4a1 C T 15: 83,974,159 (GRCm39) probably null Het
Syngap1 A G 17: 27,176,126 (GRCm39) H138R probably damaging Het
Tdrd7 A G 4: 46,034,301 (GRCm39) K1016E probably benign Het
Tmem167b T C 3: 108,467,569 (GRCm39) K26E possibly damaging Het
Tnfaip3 A G 10: 18,883,943 (GRCm39) S146P probably damaging Het
Ttn C T 2: 76,607,060 (GRCm39) V16242I possibly damaging Het
Vmn2r-ps3 T A 3: 63,960,870 (GRCm39) noncoding transcript Het
Vps13c T C 9: 67,864,838 (GRCm39) F3049S probably damaging Het
Vstm2l T C 2: 157,777,355 (GRCm39) W78R probably damaging Het
Wbp1l C A 19: 46,642,695 (GRCm39) A216D possibly damaging Het
Zer1 C G 2: 29,994,866 (GRCm39) V510L probably damaging Het
Zfp352 C A 4: 90,113,341 (GRCm39) P494T possibly damaging Het
Zfp353-ps A G 8: 42,535,600 (GRCm39) noncoding transcript Het
Zfp563 A T 17: 33,321,515 (GRCm39) probably null Het
Zim1 T C 7: 6,680,320 (GRCm39) N448D probably benign Het
Other mutations in Zfp28
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00737:Zfp28 APN 7 6,396,429 (GRCm39) makesense probably null
IGL02300:Zfp28 APN 7 6,392,495 (GRCm39) missense probably benign 0.00
IGL02541:Zfp28 APN 7 6,396,479 (GRCm39) nonsense probably null
FR4340:Zfp28 UTSW 7 6,397,862 (GRCm39) missense probably damaging 1.00
FR4342:Zfp28 UTSW 7 6,397,862 (GRCm39) missense probably damaging 1.00
R0442:Zfp28 UTSW 7 6,397,998 (GRCm39) missense probably damaging 1.00
R0462:Zfp28 UTSW 7 6,395,239 (GRCm39) missense possibly damaging 0.71
R0799:Zfp28 UTSW 7 6,387,182 (GRCm39) missense possibly damaging 0.49
R1081:Zfp28 UTSW 7 6,392,779 (GRCm39) missense possibly damaging 0.93
R1674:Zfp28 UTSW 7 6,397,942 (GRCm39) missense possibly damaging 0.90
R1783:Zfp28 UTSW 7 6,397,791 (GRCm39) missense probably damaging 1.00
R2119:Zfp28 UTSW 7 6,397,875 (GRCm39) missense probably benign 0.00
R2186:Zfp28 UTSW 7 6,397,497 (GRCm39) missense probably damaging 1.00
R4280:Zfp28 UTSW 7 6,396,700 (GRCm39) missense probably benign 0.07
R4281:Zfp28 UTSW 7 6,396,700 (GRCm39) missense probably benign 0.07
R4283:Zfp28 UTSW 7 6,396,700 (GRCm39) missense probably benign 0.07
R4331:Zfp28 UTSW 7 6,396,700 (GRCm39) missense probably benign 0.07
R4379:Zfp28 UTSW 7 6,396,441 (GRCm39) missense probably benign 0.11
R4380:Zfp28 UTSW 7 6,396,441 (GRCm39) missense probably benign 0.11
R4505:Zfp28 UTSW 7 6,397,160 (GRCm39) missense probably damaging 1.00
R4659:Zfp28 UTSW 7 6,396,506 (GRCm39) missense probably benign 0.05
R4706:Zfp28 UTSW 7 6,392,793 (GRCm39) missense probably damaging 0.99
R6269:Zfp28 UTSW 7 6,396,612 (GRCm39) missense probably benign 0.00
R6981:Zfp28 UTSW 7 6,397,692 (GRCm39) missense probably damaging 1.00
R7117:Zfp28 UTSW 7 6,397,461 (GRCm39) missense probably damaging 1.00
R7176:Zfp28 UTSW 7 6,386,456 (GRCm39) missense possibly damaging 0.49
R7312:Zfp28 UTSW 7 6,386,593 (GRCm39) unclassified probably benign
R7422:Zfp28 UTSW 7 6,397,748 (GRCm39) missense probably damaging 1.00
R7423:Zfp28 UTSW 7 6,396,956 (GRCm39) missense probably damaging 1.00
R7937:Zfp28 UTSW 7 6,396,785 (GRCm39) missense probably damaging 1.00
R8110:Zfp28 UTSW 7 6,392,828 (GRCm39) missense probably benign 0.02
R8704:Zfp28 UTSW 7 6,397,637 (GRCm39) missense probably damaging 1.00
R8804:Zfp28 UTSW 7 6,393,399 (GRCm39) missense probably damaging 0.98
R8854:Zfp28 UTSW 7 6,397,938 (GRCm39) missense probably benign 0.01
R9071:Zfp28 UTSW 7 6,397,544 (GRCm39) missense probably damaging 1.00
R9235:Zfp28 UTSW 7 6,397,605 (GRCm39) missense probably damaging 1.00
R9276:Zfp28 UTSW 7 6,397,440 (GRCm39) missense probably damaging 1.00
R9589:Zfp28 UTSW 7 6,392,816 (GRCm39) missense probably benign 0.09
R9600:Zfp28 UTSW 7 6,397,917 (GRCm39) missense probably benign 0.00
R9651:Zfp28 UTSW 7 6,395,623 (GRCm39) missense
R9653:Zfp28 UTSW 7 6,395,623 (GRCm39) missense
R9712:Zfp28 UTSW 7 6,396,878 (GRCm39) missense probably damaging 1.00
Y4340:Zfp28 UTSW 7 6,397,656 (GRCm39) missense probably damaging 1.00
Z1177:Zfp28 UTSW 7 6,387,186 (GRCm39) missense probably benign 0.28
Predicted Primers PCR Primer
(F):5'- GGCCTTTGGTGATAACTCATCCTG -3'
(R):5'- TGGCAGTGAGGATGGATTCC -3'

Sequencing Primer
(F):5'- TGTACTCAACATCGGAGACTTC -3'
(R):5'- TGGATTCCAGACGAATTTGGAG -3'
Posted On 2016-10-05