Mutagenetix > Incidental Mutation

Incidental Mutation 'R6146:Tg'

501869

Institutional Source

Beutler Lab

Gene Symbol

Ensembl Gene

ENSMUSG00000053469

Gene Name

thyroglobulin

Synonyms

Tgn

MMRRC Submission

044293-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R6146 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

66542606-66722570 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 66545216 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000070239 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065916] [ENSMUST00000065916] [ENSMUST00000065916]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000065916

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000065916

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000065916

SMART Domains

Protein: ENSMUSP00000070239
Gene: ENSMUSG00000053469

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
TY	50	97	5.9e-16	SMART
TY	118	165	5.59e-17	SMART
Pfam:Thyroglobulin_1	174	252	4e-9	PFAM
TY	317	363	4.36e-19	SMART
low complexity region	495	504	N/A	INTRINSIC
TY	617	662	3.58e-15	SMART
TY	684	730	1.47e-16	SMART
TY	880	926	1.51e-4	SMART
TY	1029	1078	1.21e-12	SMART
TY	1106	1150	7.56e-5	SMART
TY	1167	1215	7.26e-16	SMART
low complexity region	1244	1255	N/A	INTRINSIC
Pfam:GCC2_GCC3	1464	1509	2.7e-16	PFAM
TY	1519	1568	9.81e-13	SMART
Pfam:COesterase	2181	2717	8.4e-140	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
PHENOTYPE: Mice homozygous for a spontaneous mutation exhibit enlarged thyroid gland, hypothyroidism, abnormal thyroid gland morphology, and decreased body weight. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb4	A	G	5: 8,946,587 (GRCm39)	E29G	probably benign	Het
Abi3bp	T	C	16: 56,491,628 (GRCm39)	S552P	probably damaging	Het
Adgrg7	A	G	16: 56,593,829 (GRCm39)	I129T	probably benign	Het
Adhfe1	A	G	1: 9,623,943 (GRCm39)	N148S	probably damaging	Het
AI182371	A	G	2: 34,987,983 (GRCm39)	Y77H	probably damaging	Het
Aldh5a1	C	T	13: 25,103,661 (GRCm39)		probably null	Het
Ankrd2	A	G	19: 42,028,544 (GRCm39)	T67A	possibly damaging	Het
Anln	A	T	9: 22,287,604 (GRCm39)	C232*	probably null	Het
Cchcr1	A	G	17: 35,839,475 (GRCm39)	D587G	possibly damaging	Het
Cgn	G	T	3: 94,674,435 (GRCm39)	Q901K	possibly damaging	Het
Cip2a	A	T	16: 48,814,692 (GRCm39)	K18*	probably null	Het
Cluh	G	T	11: 74,558,054 (GRCm39)		probably null	Het
Crebbp	G	A	16: 3,902,487 (GRCm39)	Q2213*	probably null	Het
Cyp2d22	T	A	15: 82,258,036 (GRCm39)		probably null	Het
Dcdc2a	T	C	13: 25,389,440 (GRCm39)	V456A	probably benign	Het
Depdc5	A	G	5: 33,126,075 (GRCm39)	E569G	probably benign	Het
Dnah5	T	C	15: 28,459,331 (GRCm39)	F4517L	probably benign	Het
Doc2b	T	C	11: 75,664,421 (GRCm39)	K317E	probably damaging	Het
Dysf	T	A	6: 84,180,181 (GRCm39)	D1951E	probably damaging	Het
Epha6	C	A	16: 60,245,198 (GRCm39)	A334S	possibly damaging	Het
F2rl2	A	C	13: 95,837,149 (GRCm39)	I65L	probably benign	Het
Fbxw17	A	G	13: 50,586,548 (GRCm39)	K417E	probably benign	Het
Fkbpl	G	A	17: 34,864,303 (GRCm39)	A24T	probably benign	Het
Fxr2	A	G	11: 69,532,165 (GRCm39)	M96V	possibly damaging	Het
Kcnj10	C	A	1: 172,196,892 (GRCm39)	Y135*	probably null	Het
Kidins220	G	A	12: 25,102,812 (GRCm39)	R1238Q	probably damaging	Het
Krt31	T	C	11: 99,939,056 (GRCm39)	N255S	probably benign	Het
Lrp2	T	C	2: 69,341,345 (GRCm39)	D945G	probably benign	Het
Lrrc66	T	C	5: 73,765,432 (GRCm39)	D537G	probably benign	Het
Ltbp4	T	A	7: 27,019,149 (GRCm39)	I992F	probably damaging	Het
Lzts1	A	G	8: 69,593,524 (GRCm39)	S28P	probably benign	Het
Mrc2	C	A	11: 105,216,470 (GRCm39)	N86K	probably damaging	Het
Mroh6	C	A	15: 75,758,486 (GRCm39)	A302S	possibly damaging	Het
Muc16	T	A	9: 18,409,093 (GRCm39)	N198Y	probably damaging	Het
Myo9a	T	C	9: 59,778,512 (GRCm39)	S1423P	probably benign	Het
Or10ag58	A	T	2: 87,265,662 (GRCm39)	D277V	possibly damaging	Het
Or2b28	A	T	13: 21,531,164 (GRCm39)	Y22F	possibly damaging	Het
Or5h22	A	G	16: 58,895,077 (GRCm39)	V122A	probably benign	Het
Or5p56	G	T	7: 107,589,620 (GRCm39)	G16V	probably damaging	Het
Or5w17	A	C	2: 87,583,602 (GRCm39)	L245R	probably damaging	Het
Or9g4	T	A	2: 85,504,938 (GRCm39)	K186*	probably null	Het
Otogl	G	A	10: 107,612,978 (GRCm39)		silent	Het
Polg	T	C	7: 79,100,260 (GRCm39)	M1184V	probably benign	Het
Prl8a8	T	C	13: 27,694,463 (GRCm39)	Y108C	probably damaging	Het
Proser1	T	A	3: 53,385,540 (GRCm39)	I474N	probably damaging	Het
Rab44	A	G	17: 29,354,391 (GRCm39)		probably benign	Het
Rbp1	C	A	9: 98,307,669 (GRCm39)	D79E	possibly damaging	Het
Rnf213	T	C	11: 119,326,825 (GRCm39)	V1605A	probably benign	Het
Rps24	A	T	14: 24,540,803 (GRCm39)		probably null	Het
Setd5	T	C	6: 113,098,773 (GRCm39)		probably null	Het
Skic3	A	G	13: 76,333,359 (GRCm39)	E1536G	probably damaging	Het
Slc38a3	T	C	9: 107,532,228 (GRCm39)	I435V	probably benign	Het
Slco1a5	T	A	6: 142,180,534 (GRCm39)	M623L	probably benign	Het
Spaca9	G	A	2: 28,583,793 (GRCm39)	R64W	probably damaging	Het
Spn	A	G	7: 126,735,479 (GRCm39)	S343P	possibly damaging	Het
Sptan1	A	G	2: 29,894,535 (GRCm39)	T1168A	probably benign	Het
Sptbn4	A	T	7: 27,064,012 (GRCm39)	L2138*	probably null	Het
Tigd5	T	A	15: 75,782,094 (GRCm39)	L152Q	probably damaging	Het
Tmem163	C	T	1: 127,447,126 (GRCm39)	V170I	probably benign	Het
Tpr	T	C	1: 150,298,913 (GRCm39)	C1068R	possibly damaging	Het
Ubr1	A	T	2: 120,723,690 (GRCm39)	Y1290N	probably damaging	Het
Vmn1r184	T	A	7: 25,966,817 (GRCm39)	F188I	probably benign	Het
Vmn2r100	G	A	17: 19,742,522 (GRCm39)	V299I	probably benign	Het
Vmn2r91	T	G	17: 18,356,518 (GRCm39)	H728Q	probably benign	Het
Vta1	T	C	10: 14,581,096 (GRCm39)	Y37C	probably damaging	Het
Wbp2	C	A	11: 115,974,728 (GRCm39)	M35I	probably benign	Het
Zcchc9	G	A	13: 91,953,986 (GRCm39)	Q90*	probably null	Het

Other mutations in Tg

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Tg	APN	15	66,719,015 (GRCm39)	missense	probably damaging	1.00
IGL00230:Tg	APN	15	66,699,139 (GRCm39)	missense	probably benign	0.00
IGL00324:Tg	APN	15	66,565,273 (GRCm39)	missense	probably benign
IGL00428:Tg	APN	15	66,645,273 (GRCm39)	missense	probably benign	0.33
IGL00703:Tg	APN	15	66,568,338 (GRCm39)	missense	probably benign	0.34
IGL00808:Tg	APN	15	66,555,662 (GRCm39)	missense	probably damaging	1.00
IGL00833:Tg	APN	15	66,560,650 (GRCm39)	missense	probably benign	0.34
IGL00899:Tg	APN	15	66,545,922 (GRCm39)	critical splice donor site	probably null
IGL00921:Tg	APN	15	66,636,302 (GRCm39)	missense	probably benign	0.28
IGL00975:Tg	APN	15	66,553,731 (GRCm39)	missense	probably benign
IGL01288:Tg	APN	15	66,608,125 (GRCm39)	missense	possibly damaging	0.81
IGL01397:Tg	APN	15	66,567,941 (GRCm39)	splice site	probably benign
IGL01634:Tg	APN	15	66,601,415 (GRCm39)	missense	probably benign	0.34
IGL01646:Tg	APN	15	66,549,936 (GRCm39)	missense	probably damaging	1.00
IGL01704:Tg	APN	15	66,543,200 (GRCm39)	missense	probably damaging	0.98
IGL01958:Tg	APN	15	66,631,335 (GRCm39)	missense	probably benign	0.06
IGL02093:Tg	APN	15	66,564,223 (GRCm39)	missense	possibly damaging	0.83
IGL02113:Tg	APN	15	66,577,179 (GRCm39)	missense	probably benign	0.08
IGL02138:Tg	APN	15	66,589,082 (GRCm39)	missense	probably benign	0.01
IGL02156:Tg	APN	15	66,577,197 (GRCm39)	missense	probably benign	0.19
IGL02169:Tg	APN	15	66,629,792 (GRCm39)	missense	probably benign	0.04
IGL02342:Tg	APN	15	66,636,140 (GRCm39)	missense	probably benign
IGL02434:Tg	APN	15	66,636,191 (GRCm39)	missense	probably damaging	0.97
IGL02506:Tg	APN	15	66,613,443 (GRCm39)	missense	possibly damaging	0.71
IGL02513:Tg	APN	15	66,577,123 (GRCm39)	missense	probably benign
IGL02549:Tg	APN	15	66,711,210 (GRCm39)	missense	probably damaging	1.00
IGL02669:Tg	APN	15	66,620,575 (GRCm39)	splice site	probably benign
IGL02756:Tg	APN	15	66,606,435 (GRCm39)	missense	probably benign
IGL02800:Tg	APN	15	66,629,735 (GRCm39)	missense	probably damaging	1.00
IGL02828:Tg	APN	15	66,554,243 (GRCm39)	missense	probably damaging	1.00
IGL02927:Tg	APN	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
IGL03061:Tg	APN	15	66,543,254 (GRCm39)	missense	probably damaging	1.00
IGL03105:Tg	APN	15	66,586,955 (GRCm39)	missense	probably benign	0.01
IGL03160:Tg	APN	15	66,711,152 (GRCm39)	nonsense	probably null
IGL03242:Tg	APN	15	66,555,647 (GRCm39)	missense	probably damaging	0.99
Also_ran	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
bedraggled	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
foster	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
hognose	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
ito	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
ito2	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
ito3	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
ito4	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
Papua	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
Pipistrella	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
pluribus	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
samarai	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
sariba	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
ticker	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
Vampire	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
IGL03134:Tg	UTSW	15	66,612,567 (GRCm39)	missense	probably damaging	1.00
P0019:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
R0121:Tg	UTSW	15	66,612,630 (GRCm39)	missense	probably benign	0.04
R0135:Tg	UTSW	15	66,566,719 (GRCm39)	missense	probably benign	0.01
R0227:Tg	UTSW	15	66,570,295 (GRCm39)	missense	possibly damaging	0.84
R0448:Tg	UTSW	15	66,636,291 (GRCm39)	missense	probably damaging	1.00
R0453:Tg	UTSW	15	66,700,382 (GRCm39)	missense	probably benign	0.09
R0504:Tg	UTSW	15	66,554,253 (GRCm39)	missense	probably damaging	0.97
R0543:Tg	UTSW	15	66,601,446 (GRCm39)	missense	probably benign	0.13
R0638:Tg	UTSW	15	66,589,057 (GRCm39)	missense	probably damaging	0.99
R0639:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0646:Tg	UTSW	15	66,601,475 (GRCm39)	missense	probably damaging	0.99
R0666:Tg	UTSW	15	66,609,370 (GRCm39)	missense	probably benign
R0673:Tg	UTSW	15	66,613,333 (GRCm39)	critical splice acceptor site	probably null
R0689:Tg	UTSW	15	66,711,253 (GRCm39)	splice site	probably benign
R0704:Tg	UTSW	15	66,629,729 (GRCm39)	missense	probably benign	0.02
R0730:Tg	UTSW	15	66,550,638 (GRCm39)	missense	probably damaging	1.00
R0830:Tg	UTSW	15	66,596,993 (GRCm39)	missense	probably damaging	1.00
R0959:Tg	UTSW	15	66,579,859 (GRCm39)	missense	probably damaging	0.98
R1027:Tg	UTSW	15	66,544,258 (GRCm39)	missense	possibly damaging	0.65
R1061:Tg	UTSW	15	66,570,408 (GRCm39)	missense	probably benign	0.09
R1086:Tg	UTSW	15	66,555,911 (GRCm39)	missense	probably benign
R1103:Tg	UTSW	15	66,591,504 (GRCm39)	missense	probably benign	0.45
R1240:Tg	UTSW	15	66,700,397 (GRCm39)	missense	probably benign	0.16
R1281:Tg	UTSW	15	66,568,338 (GRCm39)	missense	probably benign	0.34
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1470:Tg	UTSW	15	66,721,312 (GRCm39)	missense	possibly damaging	0.95
R1531:Tg	UTSW	15	66,722,351 (GRCm39)	missense	probably benign	0.02
R1544:Tg	UTSW	15	66,577,081 (GRCm39)	missense	probably benign	0.04
R1550:Tg	UTSW	15	66,565,279 (GRCm39)	missense	possibly damaging	0.52
R1575:Tg	UTSW	15	66,601,534 (GRCm39)	critical splice donor site	probably null
R1638:Tg	UTSW	15	66,568,015 (GRCm39)	nonsense	probably null
R1655:Tg	UTSW	15	66,700,417 (GRCm39)	critical splice donor site	probably null
R1671:Tg	UTSW	15	66,564,236 (GRCm39)	missense	possibly damaging	0.89
R1789:Tg	UTSW	15	66,609,397 (GRCm39)	missense	probably benign	0.00
R1883:Tg	UTSW	15	66,543,158 (GRCm39)	missense	probably damaging	1.00
R1984:Tg	UTSW	15	66,554,691 (GRCm39)	missense	probably benign
R2063:Tg	UTSW	15	66,700,402 (GRCm39)	missense	probably damaging	1.00
R2092:Tg	UTSW	15	66,721,456 (GRCm39)	missense	probably null	0.26
R2109:Tg	UTSW	15	66,601,443 (GRCm39)	missense	probably benign	0.02
R2128:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2129:Tg	UTSW	15	66,566,743 (GRCm39)	missense	probably benign	0.10
R2207:Tg	UTSW	15	66,553,788 (GRCm39)	missense	probably benign	0.15
R2219:Tg	UTSW	15	66,553,782 (GRCm39)	missense	probably benign	0.03
R2228:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2229:Tg	UTSW	15	66,545,860 (GRCm39)	missense	probably damaging	0.99
R2259:Tg	UTSW	15	66,555,747 (GRCm39)	missense	probably benign
R2994:Tg	UTSW	15	66,553,802 (GRCm39)	missense	probably benign
R3904:Tg	UTSW	15	66,638,011 (GRCm39)	nonsense	probably null
R3946:Tg	UTSW	15	66,545,872 (GRCm39)	missense	probably damaging	1.00
R3965:Tg	UTSW	15	66,556,039 (GRCm39)	missense	probably benign
R4245:Tg	UTSW	15	66,568,318 (GRCm39)	missense	possibly damaging	0.68
R4451:Tg	UTSW	15	66,637,996 (GRCm39)	missense	probably benign	0.01
R4487:Tg	UTSW	15	66,543,245 (GRCm39)	missense	probably damaging	1.00
R4489:Tg	UTSW	15	66,579,791 (GRCm39)	missense	probably damaging	1.00
R4623:Tg	UTSW	15	66,607,120 (GRCm39)	missense	probably benign	0.23
R4659:Tg	UTSW	15	66,545,769 (GRCm39)	missense	possibly damaging	0.67
R4728:Tg	UTSW	15	66,554,676 (GRCm39)	missense	probably damaging	1.00
R4760:Tg	UTSW	15	66,565,168 (GRCm39)	missense	probably damaging	1.00
R4797:Tg	UTSW	15	66,629,855 (GRCm39)	critical splice donor site	probably null
R4944:Tg	UTSW	15	66,636,186 (GRCm39)	missense	probably damaging	1.00
R4998:Tg	UTSW	15	66,545,899 (GRCm39)	missense	probably damaging	1.00
R5009:Tg	UTSW	15	66,568,435 (GRCm39)	missense	probably benign	0.01
R5025:Tg	UTSW	15	66,579,779 (GRCm39)	missense	probably damaging	1.00
R5035:Tg	UTSW	15	66,553,662 (GRCm39)	splice site	probably null
R5049:Tg	UTSW	15	66,699,231 (GRCm39)	nonsense	probably null
R5073:Tg	UTSW	15	66,607,101 (GRCm39)	missense	probably benign	0.05
R5169:Tg	UTSW	15	66,550,629 (GRCm39)	nonsense	probably null
R5185:Tg	UTSW	15	66,645,323 (GRCm39)	missense	probably damaging	1.00
R5227:Tg	UTSW	15	66,631,416 (GRCm39)	missense	possibly damaging	0.87
R5300:Tg	UTSW	15	66,550,704 (GRCm39)	missense	probably damaging	1.00
R5334:Tg	UTSW	15	66,549,904 (GRCm39)	missense	probably damaging	1.00
R5339:Tg	UTSW	15	66,549,942 (GRCm39)	missense	probably damaging	1.00
R5402:Tg	UTSW	15	66,611,017 (GRCm39)	missense	probably damaging	0.98
R5441:Tg	UTSW	15	66,568,369 (GRCm39)	missense	possibly damaging	0.47
R5509:Tg	UTSW	15	66,699,142 (GRCm39)	missense	probably benign	0.45
R5580:Tg	UTSW	15	66,557,149 (GRCm39)	missense	possibly damaging	0.66
R5582:Tg	UTSW	15	66,565,284 (GRCm39)	missense	probably damaging	1.00
R5624:Tg	UTSW	15	66,709,906 (GRCm39)	missense	probably benign	0.11
R5686:Tg	UTSW	15	66,560,738 (GRCm39)	missense	probably benign	0.28
R6042:Tg	UTSW	15	66,555,842 (GRCm39)	missense	probably benign	0.01
R6122:Tg	UTSW	15	66,700,306 (GRCm39)	missense	probably damaging	1.00
R6159:Tg	UTSW	15	66,607,096 (GRCm39)	missense	possibly damaging	0.71
R6223:Tg	UTSW	15	66,579,771 (GRCm39)	missense	probably benign	0.15
R6480:Tg	UTSW	15	66,543,160 (GRCm39)	missense	probably damaging	1.00
R6505:Tg	UTSW	15	66,631,407 (GRCm39)	missense	probably damaging	0.99
R6531:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	0.99
R6614:Tg	UTSW	15	66,607,108 (GRCm39)	missense	probably damaging	0.99
R6698:Tg	UTSW	15	66,711,211 (GRCm39)	missense	probably damaging	1.00
R6798:Tg	UTSW	15	66,550,688 (GRCm39)	missense	probably damaging	1.00
R6837:Tg	UTSW	15	66,567,984 (GRCm39)	missense	probably damaging	1.00
R6861:Tg	UTSW	15	66,560,740 (GRCm39)	missense	probably benign	0.00
R6888:Tg	UTSW	15	66,568,095 (GRCm39)	missense	probably damaging	0.99
R6933:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R6983:Tg	UTSW	15	66,565,207 (GRCm39)	missense	probably benign	0.01
R7078:Tg	UTSW	15	66,545,392 (GRCm39)	missense	probably damaging	1.00
R7244:Tg	UTSW	15	66,612,563 (GRCm39)	missense	probably damaging	1.00
R7320:Tg	UTSW	15	66,566,633 (GRCm39)	missense	possibly damaging	0.71
R7334:Tg	UTSW	15	66,597,121 (GRCm39)	missense	probably benign	0.01
R7418:Tg	UTSW	15	66,568,432 (GRCm39)	missense	probably damaging	0.99
R7485:Tg	UTSW	15	66,568,437 (GRCm39)	missense	probably benign	0.04
R7524:Tg	UTSW	15	66,568,010 (GRCm39)	missense	probably benign	0.01
R7529:Tg	UTSW	15	66,566,617 (GRCm39)	missense	probably damaging	0.99
R7540:Tg	UTSW	15	66,561,776 (GRCm39)	missense	probably benign	0.16
R7583:Tg	UTSW	15	66,636,267 (GRCm39)	missense	probably damaging	1.00
R7594:Tg	UTSW	15	66,601,432 (GRCm39)	missense	probably benign	0.20
R7667:Tg	UTSW	15	66,587,012 (GRCm39)	missense	probably damaging	0.98
R7722:Tg	UTSW	15	66,636,158 (GRCm39)	missense	possibly damaging	0.73
R7790:Tg	UTSW	15	66,721,453 (GRCm39)	missense	probably damaging	0.99
R7838:Tg	UTSW	15	66,565,112 (GRCm39)	missense	probably benign	0.00
R7890:Tg	UTSW	15	66,555,663 (GRCm39)	missense	probably damaging	1.00
R7904:Tg	UTSW	15	66,577,128 (GRCm39)	missense	probably benign	0.08
R7919:Tg	UTSW	15	66,555,923 (GRCm39)	missense	possibly damaging	0.73
R7921:Tg	UTSW	15	66,555,642 (GRCm39)	missense	probably benign	0.08
R8037:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8038:Tg	UTSW	15	66,560,724 (GRCm39)	missense	probably benign	0.00
R8214:Tg	UTSW	15	66,645,247 (GRCm39)	missense	probably damaging	1.00
R8304:Tg	UTSW	15	66,565,109 (GRCm39)	nonsense	probably null
R8688:Tg	UTSW	15	66,566,802 (GRCm39)	critical splice donor site	probably benign
R8709:Tg	UTSW	15	66,553,786 (GRCm39)	missense	probably benign	0.08
R8714:Tg	UTSW	15	66,555,891 (GRCm39)	missense	probably damaging	0.97
R8901:Tg	UTSW	15	66,557,184 (GRCm39)	missense	probably damaging	1.00
R8917:Tg	UTSW	15	66,645,332 (GRCm39)	critical splice donor site	probably null
R9023:Tg	UTSW	15	66,555,522 (GRCm39)	missense	probably damaging	1.00
R9232:Tg	UTSW	15	66,570,310 (GRCm39)	missense	probably benign	0.01
R9310:Tg	UTSW	15	66,699,118 (GRCm39)	missense	possibly damaging	0.69
R9361:Tg	UTSW	15	66,557,246 (GRCm39)	missense	possibly damaging	0.50
R9389:Tg	UTSW	15	66,561,173 (GRCm39)	missense	probably benign	0.04
R9501:Tg	UTSW	15	66,718,923 (GRCm39)	missense	possibly damaging	0.52
R9510:Tg	UTSW	15	66,545,913 (GRCm39)	missense	probably damaging	1.00
R9594:Tg	UTSW	15	66,607,109 (GRCm39)	nonsense	probably null
R9629:Tg	UTSW	15	66,555,587 (GRCm39)	missense	possibly damaging	0.95
R9701:Tg	UTSW	15	66,637,991 (GRCm39)	missense	probably benign	0.03
R9743:Tg	UTSW	15	66,561,839 (GRCm39)	missense	probably benign	0.18
R9748:Tg	UTSW	15	66,719,008 (GRCm39)	missense	possibly damaging	0.91
T0975:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0005:Tg	UTSW	15	66,560,712 (GRCm39)	missense	probably benign	0.01
X0065:Tg	UTSW	15	66,554,303 (GRCm39)	missense	probably damaging	1.00
X0067:Tg	UTSW	15	66,620,592 (GRCm39)	missense	probably benign	0.10
Z1177:Tg	UTSW	15	66,721,396 (GRCm39)	missense	probably benign	0.02
Z1177:Tg	UTSW	15	66,557,159 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TCGGTTAACCCTACAGTGCC -3'
(R):5'- TACTTACACCGAGTTGGCCTG -3'

Sequencing Primer
(F):5'- CGGGGCACTAGTGAGAGC -3'
(R):5'- AGTTGGCCTGCCCTGCTG -3'

Posted On

2017-12-04