Mutagenetix > Incidental Mutation

Incidental Mutation 'R6195:Or8k25'

502830

Institutional Source

Beutler Lab

Gene Symbol

Or8k25

Ensembl Gene

ENSMUSG00000075185

Gene Name

olfactory receptor family 8 subfamily K member 25

Synonyms

MOR188-1, MOR188-9, Olfr1515, Olfr1061, MOR188-1, MOR188-7, GA_x6K02T2Q125-47883395-47882454

MMRRC Submission

044335-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R6195 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

86243453-86244394 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 86243551 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 282 (I282V)

Ref Sequence

ENSEMBL: ENSMUSP00000097474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099889]

AlphaFold

Q7TR72

Predicted Effect

probably damaging
Transcript: ENSMUST00000099889
AA Change: I282V

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000097474
Gene: ENSMUSG00000075185
AA Change: I282V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.5e-51	PFAM
Pfam:7tm_1	41	291	2.2e-17	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

97% (76/78)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A130010J15Rik	A	G	1: 192,857,142 (GRCm39)		probably null	Het
Aadacl2fm2	G	A	3: 59,659,623 (GRCm39)	V359I	probably damaging	Het
Abtb1	T	C	6: 88,817,718 (GRCm39)	E50G	probably benign	Het
Agbl2	T	A	2: 90,643,657 (GRCm39)	D792E	probably benign	Het
Aoc1	C	A	6: 48,885,611 (GRCm39)	N705K	probably damaging	Het
Araf	G	T	X: 20,726,339 (GRCm39)	R601L	probably damaging	Homo
Arhgef7	C	T	8: 11,872,017 (GRCm39)	T701I	probably damaging	Het
Atg10	G	T	13: 91,356,555 (GRCm39)		probably null	Het
Atp5f1c	T	C	2: 10,068,926 (GRCm39)	I116M	possibly damaging	Het
Baz2b	T	A	2: 59,737,855 (GRCm39)	Q1818L	possibly damaging	Het
Bod1	A	G	11: 31,616,740 (GRCm39)	*174Q	probably null	Het
Cacna1a	A	G	8: 85,315,382 (GRCm39)	Y1539C	probably damaging	Het
Creb3	A	G	4: 43,566,346 (GRCm39)	D260G	probably benign	Het
Cyp1b1	G	T	17: 80,021,695 (GRCm39)	L16M	probably damaging	Het
Dhx29	T	A	13: 113,101,071 (GRCm39)	S1205T	probably benign	Het
Dlec1	A	G	9: 118,966,321 (GRCm39)	K1097E	probably benign	Het
Dnah7b	G	A	1: 46,243,429 (GRCm39)	D1578N	probably damaging	Het
Dok3	T	C	13: 55,671,389 (GRCm39)	N394S	probably benign	Het
Dpcd	A	G	19: 45,565,458 (GRCm39)	D144G	probably damaging	Het
Efhb	A	G	17: 53,769,580 (GRCm39)	F243S	possibly damaging	Het
Eif2ak2	A	T	17: 79,178,662 (GRCm39)	Y137*	probably null	Het
Eif4e1b	A	G	13: 54,932,018 (GRCm39)	N34S	probably null	Het
F2rl3	A	G	8: 73,489,513 (GRCm39)	T247A	probably benign	Het
Fan1	A	T	7: 64,004,119 (GRCm39)	H782Q	probably damaging	Het
Fer1l5	T	C	1: 36,414,367 (GRCm39)		probably null	Het
Fer1l6	T	C	15: 58,509,806 (GRCm39)	S1423P	probably damaging	Het
Fetub	C	T	16: 22,751,081 (GRCm39)	R143C	probably damaging	Het
Fgfbp1	T	C	5: 44,136,704 (GRCm39)	D196G	possibly damaging	Het
Fxn	G	A	19: 24,239,407 (GRCm39)	R162C	probably damaging	Het
Fxr2	C	T	11: 69,543,099 (GRCm39)	T632M	probably benign	Het
Gab1	A	T	8: 81,606,161 (GRCm39)	Y24*	probably null	Het
Gcc2	T	C	10: 58,106,806 (GRCm39)	S681P	probably damaging	Het
Git2	T	C	5: 114,905,175 (GRCm39)	N94S	probably benign	Het
Gm5799	T	G	14: 43,782,088 (GRCm39)	L87V	probably damaging	Het
Golga7b	A	T	19: 42,251,886 (GRCm39)	D44V	probably benign	Het
Hace1	T	A	10: 45,546,539 (GRCm39)	I391N	possibly damaging	Het
Hmcn2	T	C	2: 31,274,127 (GRCm39)	S1416P	probably damaging	Het
Hoxa11	G	A	6: 52,222,681 (GRCm39)	R7C	probably damaging	Het
Igkv14-126	A	T	6: 67,873,475 (GRCm39)	T68S	possibly damaging	Het
Insyn2a	T	C	7: 134,520,377 (GRCm39)	D51G	probably damaging	Het
Itpr3	T	C	17: 27,305,934 (GRCm39)	I164T	probably damaging	Het
Kif22	A	G	7: 126,628,131 (GRCm39)	S540P	probably damaging	Het
Ldlr	T	A	9: 21,643,077 (GRCm39)	C34*	probably null	Het
Lrrtm4	T	C	6: 79,998,939 (GRCm39)	L117P	probably damaging	Het
Mad2l1	G	T	6: 66,514,612 (GRCm39)	G94C	possibly damaging	Het
Malrd1	C	A	2: 15,700,137 (GRCm39)	H661Q	probably damaging	Het
Mical3	T	A	6: 120,993,796 (GRCm39)		probably benign	Het
Mipep	T	A	14: 61,109,554 (GRCm39)	W644R	probably damaging	Het
Mycl	A	G	4: 122,893,713 (GRCm39)	D171G	probably damaging	Het
Myof	A	G	19: 37,901,805 (GRCm39)	F997L	possibly damaging	Het
Nagpa	C	T	16: 5,021,613 (GRCm39)	R46H	probably damaging	Het
Nf1	A	G	11: 79,456,801 (GRCm39)	Y629C	probably damaging	Het
Obscn	T	A	11: 58,888,033 (GRCm39)	E2164V	probably damaging	Het
Or52h1	A	T	7: 103,828,961 (GRCm39)	V218D	possibly damaging	Het
Or5ac23	A	G	16: 59,149,785 (GRCm39)	V29A	possibly damaging	Het
Or5w19	T	C	2: 87,698,904 (GRCm39)	S190P	possibly damaging	Het
Pcdh20	T	C	14: 88,705,488 (GRCm39)	E604G	probably benign	Het
Pcdhb7	G	A	18: 37,475,709 (GRCm39)	V282I	probably benign	Het
Pcnx4	A	G	12: 72,603,648 (GRCm39)	D523G	possibly damaging	Het
Pigx	G	A	16: 31,903,404 (GRCm39)	T219I	probably damaging	Het
Plch1	G	T	3: 63,648,210 (GRCm39)	P399Q	probably damaging	Het
Pvrig-ps	T	A	5: 138,340,537 (GRCm39)	F74I	possibly damaging	Het
Rsrp1	T	A	4: 134,654,113 (GRCm39)	I255K	probably damaging	Het
Scn1a	T	A	2: 66,107,962 (GRCm39)	Y1588F	possibly damaging	Het
Serpina9	T	A	12: 103,967,666 (GRCm39)	H243L	probably damaging	Het
Tapbp	T	C	17: 34,138,956 (GRCm39)	L41P	probably damaging	Het
Tbc1d16	T	A	11: 119,101,391 (GRCm39)	K40*	probably null	Het
Tbc1d2	C	T	4: 46,629,912 (GRCm39)	G252R	probably benign	Het
Tbc1d23	T	C	16: 57,051,713 (GRCm39)	E6G	possibly damaging	Het
Tdrd6	A	G	17: 43,940,643 (GRCm39)	V135A	probably damaging	Het
Tmem87a	A	T	2: 120,222,656 (GRCm39)		probably null	Het
Tnrc18	T	A	5: 142,750,928 (GRCm39)	K1217N	unknown	Het
Trim33	G	A	3: 103,244,848 (GRCm39)		probably null	Het
Ttn	T	A	2: 76,567,997 (GRCm39)	Y27632F	probably benign	Het
Tubgcp6	A	T	15: 89,006,994 (GRCm39)	D9E	probably benign	Het
Uaca	G	A	9: 60,777,326 (GRCm39)	R571Q	probably damaging	Het
Zfp655	T	A	5: 145,180,572 (GRCm39)	F143L	possibly damaging	Het

Other mutations in Or8k25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01714:Or8k25	APN	2	86,244,144 (GRCm39)	missense	probably benign	0.35
IGL01721:Or8k25	APN	2	86,243,677 (GRCm39)	missense	probably damaging	1.00
IGL02696:Or8k25	APN	2	86,243,959 (GRCm39)	missense	probably benign	0.03
BB007:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
BB017:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
R0366:Or8k25	UTSW	2	86,244,369 (GRCm39)	missense	possibly damaging	0.71
R0607:Or8k25	UTSW	2	86,243,514 (GRCm39)	missense	probably damaging	1.00
R1013:Or8k25	UTSW	2	86,244,319 (GRCm39)	missense	possibly damaging	0.61
R1017:Or8k25	UTSW	2	86,243,855 (GRCm39)	missense	probably damaging	1.00
R1617:Or8k25	UTSW	2	86,244,035 (GRCm39)	nonsense	probably null
R1690:Or8k25	UTSW	2	86,244,298 (GRCm39)	missense	probably benign	0.03
R4126:Or8k25	UTSW	2	86,243,568 (GRCm39)	missense	probably damaging	0.99
R5053:Or8k25	UTSW	2	86,243,682 (GRCm39)	missense	probably damaging	1.00
R5443:Or8k25	UTSW	2	86,243,937 (GRCm39)	missense	possibly damaging	0.54
R6233:Or8k25	UTSW	2	86,243,551 (GRCm39)	missense	probably damaging	0.98
R6468:Or8k25	UTSW	2	86,244,381 (GRCm39)	missense	probably damaging	0.99
R7188:Or8k25	UTSW	2	86,243,695 (GRCm39)	nonsense	probably null
R7300:Or8k25	UTSW	2	86,244,330 (GRCm39)	missense	probably null	0.27
R7374:Or8k25	UTSW	2	86,244,196 (GRCm39)	missense	probably benign	0.39
R7392:Or8k25	UTSW	2	86,243,496 (GRCm39)	missense	probably benign
R7494:Or8k25	UTSW	2	86,243,592 (GRCm39)	missense	probably benign	0.24
R7930:Or8k25	UTSW	2	86,243,560 (GRCm39)	missense	probably damaging	1.00
R8491:Or8k25	UTSW	2	86,244,099 (GRCm39)	missense	probably benign	0.00
R8680:Or8k25	UTSW	2	86,243,935 (GRCm39)	missense	probably benign	0.16
R8848:Or8k25	UTSW	2	86,243,821 (GRCm39)	missense	probably benign	0.05
R9175:Or8k25	UTSW	2	86,244,099 (GRCm39)	missense	probably benign	0.00
X0023:Or8k25	UTSW	2	86,244,303 (GRCm39)	missense	probably benign	0.29
Z1176:Or8k25	UTSW	2	86,243,872 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CACACAGAATTTAACCTTGGTGTTC -3'
(R):5'- GGCCTGCTCAAACACACATG -3'

Sequencing Primer
(F):5'- AGAATTTAACCTTGGTGTTCTTCTC -3'
(R):5'- TGCTCAAACACACATGAAATTGAGG -3'

Posted On

2018-02-27