Mutagenetix > Incidental Mutation

Incidental Mutation 'R6747:Sec23ip'

543569

Institutional Source

Beutler Lab

Gene Symbol

Sec23ip

Ensembl Gene

ENSMUSG00000055319

Gene Name

Sec23 interacting protein

Synonyms

p125, D7Ertd373e

MMRRC Submission

044864-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.583) question?

Stock #

R6747 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

128346667-128386560 bp(+) (GRCm39)

Type of Mutation

synonymous

DNA Base Change (assembly)

A to G at 128354573 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000035610 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042942]

AlphaFold

no structure available at present

Predicted Effect

silent
Transcript: ENSMUST00000042942

SMART Domains

Protein: ENSMUSP00000035610
Gene: ENSMUSG00000055319

Domain	Start	End	E-Value	Type
low complexity region	8	26	N/A	INTRINSIC
low complexity region	41	51	N/A	INTRINSIC
low complexity region	79	88	N/A	INTRINSIC
low complexity region	203	215	N/A	INTRINSIC
low complexity region	222	230	N/A	INTRINSIC
Blast:DDHD	513	585	8e-33	BLAST
SAM	637	702	2.18e-9	SMART
DDHD	777	987	1.33e-74	SMART

Predicted Effect

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.0%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the phosphatidic acid preferring-phospholipase A1 family. The encoded protein is localized to endoplasmic reticulum exit sites and plays a critical role in ER-Golgi transport as part of the multimeric coat protein II complex. An orthologous gene in frogs is required for normal neural crest cell development, suggesting that this gene may play a role in Waardenburg syndrome neural crest defects. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
PHENOTYPE: Male mice homozygous for a null allele display reduced fertility with globozoospermia and impaired fertilization. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	C	T	3: 121,919,962 (GRCm39)		probably null	Het
Acyp1	C	T	12: 85,325,679 (GRCm39)	V107I	probably null	Het
Aftph	T	C	11: 20,676,144 (GRCm39)		probably null	Het
Agtpbp1	A	T	13: 59,692,167 (GRCm39)		probably null	Het
Arhgap15	C	A	2: 44,006,689 (GRCm39)	P269T	probably damaging	Het
Arhgap30	T	C	1: 171,235,297 (GRCm39)	V557A	probably damaging	Het
Asb3	T	A	11: 31,031,493 (GRCm39)	M410K	probably benign	Het
B4galnt2	T	C	11: 95,759,460 (GRCm39)		probably null	Het
Birc2	A	G	9: 7,860,262 (GRCm39)		probably null	Het
Cav2	A	T	6: 17,286,950 (GRCm39)	N69Y	probably damaging	Het
Cc2d2b	G	A	19: 40,784,111 (GRCm39)	C826Y	probably benign	Het
Cenpf	T	A	1: 189,385,051 (GRCm39)	T2410S	probably benign	Het
Chchd1	A	G	14: 20,753,448 (GRCm39)	D24G	probably benign	Het
Cmip	G	A	8: 118,163,618 (GRCm39)	G450S	probably benign	Het
Col11a1	C	T	3: 114,006,099 (GRCm39)	Q534*	probably null	Het
Col3a1	G	T	1: 45,377,782 (GRCm39)		probably benign	Het
Cox4i1	T	C	8: 121,399,969 (GRCm39)	I31T	possibly damaging	Het
Cstf3	G	T	2: 104,477,112 (GRCm39)	V168F	probably damaging	Het
Dapk1	T	C	13: 60,873,154 (GRCm39)	I352T	probably benign	Het
Ddx52	T	C	11: 83,846,128 (GRCm39)	V456A	probably damaging	Het
Dmxl2	A	T	9: 54,323,372 (GRCm39)	H1337Q	probably damaging	Het
Dnah7a	T	C	1: 53,675,221 (GRCm39)	T369A	probably benign	Het
Dppa1	T	A	11: 46,516,422 (GRCm39)	I8F	unknown	Het
Ebf1	G	T	11: 44,774,641 (GRCm39)	V213F	probably damaging	Het
Foxb2	C	A	19: 16,850,197 (GRCm39)	E270*	probably null	Het
Gclc	A	T	9: 77,695,527 (GRCm39)	R450W	probably damaging	Het
Grin2d	T	C	7: 45,511,692 (GRCm39)	E251G	probably damaging	Het
Hal	A	T	10: 93,336,539 (GRCm39)	N423Y	probably damaging	Het
Krtap4-8	T	A	11: 99,670,917 (GRCm39)	I185F	unknown	Het
Lrp3	T	A	7: 34,910,862 (GRCm39)	Q61L	probably benign	Het
Met	A	C	6: 17,571,466 (GRCm39)	Q1296H	probably damaging	Het
Mphosph9	T	A	5: 124,435,762 (GRCm39)	N557I	possibly damaging	Het
Mrpl46	C	A	7: 78,432,729 (GRCm39)	W16C	probably benign	Het
Myh13	G	A	11: 67,241,245 (GRCm39)	R874Q	probably damaging	Het
Nelfb	G	A	2: 25,093,393 (GRCm39)	T453I	probably benign	Het
Nos2	A	G	11: 78,843,780 (GRCm39)	D909G	probably damaging	Het
Nr5a2	T	C	1: 136,810,082 (GRCm39)	E431G	possibly damaging	Het
Nsmce2	A	G	15: 59,463,573 (GRCm39)	D149G	probably benign	Het
Or52b1	C	T	7: 104,979,234 (GRCm39)	R55H	probably benign	Het
Or5ac21	T	C	16: 59,124,004 (GRCm39)	F164L	probably benign	Het
Pcbp4	C	A	9: 106,337,847 (GRCm39)		probably null	Het
Peg10	A	G	6: 4,757,137 (GRCm39)		probably benign	Het
Pms2	C	T	5: 143,862,237 (GRCm39)	P154L	probably benign	Het
Pou6f2	A	C	13: 18,303,772 (GRCm39)	L112R	probably benign	Het
Pramel30	T	C	4: 144,059,548 (GRCm39)	W420R	probably benign	Het
Prdm6	A	T	18: 53,598,118 (GRCm39)		probably benign	Het
Prob1	G	A	18: 35,788,207 (GRCm39)	R12W	probably damaging	Het
Rif1	T	A	2: 51,968,275 (GRCm39)		probably null	Het
Rpl9-ps6	A	G	19: 32,443,543 (GRCm39)	S137P	probably benign	Het
Rsf1	GGCG	GGCGACGGCAGCG	7: 97,229,113 (GRCm39)		probably benign	Homo
Slc38a9	T	C	13: 112,826,714 (GRCm39)	C151R	probably benign	Het
Slc39a8	T	C	3: 135,554,941 (GRCm39)		probably null	Het
Slc6a18	C	A	13: 73,826,110 (GRCm39)		probably benign	Het
Snw1	T	C	12: 87,511,480 (GRCm39)	D57G	probably damaging	Het
Sox6	C	G	7: 115,140,966 (GRCm39)	R505P	probably damaging	Het
Speg	T	C	1: 75,387,039 (GRCm39)		probably null	Het
Spire2	C	T	8: 124,083,585 (GRCm39)	R190C	probably damaging	Het
Stard9	A	C	2: 120,528,864 (GRCm39)	H1707P	possibly damaging	Het
Tenm3	G	T	8: 48,796,278 (GRCm39)	T237K	probably damaging	Het
Themis2	T	A	4: 132,523,573 (GRCm39)	E31V	possibly damaging	Het
Trim56	T	G	5: 137,143,375 (GRCm39)	Q47P	probably damaging	Het
Trim58	T	C	11: 58,542,090 (GRCm39)	I350T	probably benign	Het
Ttll7	C	T	3: 146,649,811 (GRCm39)	P639S	probably benign	Het
Ubxn6	A	G	17: 56,377,650 (GRCm39)		probably null	Het
Vmn2r114	A	T	17: 23,528,850 (GRCm39)	F417L	probably benign	Het
Vmn2r29	T	A	7: 7,234,421 (GRCm39)	M822L	probably benign	Het
Vps9d1	T	C	8: 123,980,746 (GRCm39)	D27G	probably damaging	Het
Wdr3	C	T	3: 100,046,040 (GRCm39)	R931Q	probably damaging	Het
Whamm	G	A	7: 81,228,050 (GRCm39)		probably null	Het
Zcchc24	T	C	14: 25,757,457 (GRCm39)	H142R	probably damaging	Het
Zfp292	T	C	4: 34,806,894 (GRCm39)	K2050R	probably damaging	Het

Other mutations in Sec23ip

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00825:Sec23ip	APN	7	128,369,333 (GRCm39)	missense	probably damaging	1.00
IGL01347:Sec23ip	APN	7	128,364,129 (GRCm39)	missense	probably benign	0.08
IGL01358:Sec23ip	APN	7	128,354,521 (GRCm39)	missense	possibly damaging	0.68
IGL01656:Sec23ip	APN	7	128,351,969 (GRCm39)	missense	probably damaging	1.00
IGL01835:Sec23ip	APN	7	128,357,035 (GRCm39)	splice site	probably null
IGL02233:Sec23ip	APN	7	128,380,903 (GRCm39)	missense	probably damaging	1.00
IGL02499:Sec23ip	APN	7	128,378,640 (GRCm39)	missense	probably damaging	1.00
IGL03381:Sec23ip	APN	7	128,352,029 (GRCm39)	missense	probably damaging	0.97
R0053:Sec23ip	UTSW	7	128,346,891 (GRCm39)	missense	probably damaging	1.00
R0147:Sec23ip	UTSW	7	128,380,775 (GRCm39)	splice site	probably benign
R0360:Sec23ip	UTSW	7	128,363,129 (GRCm39)	splice site	probably benign
R1427:Sec23ip	UTSW	7	128,378,609 (GRCm39)	missense	probably damaging	0.99
R1442:Sec23ip	UTSW	7	128,378,510 (GRCm39)	missense	probably benign	0.10
R1462:Sec23ip	UTSW	7	128,367,862 (GRCm39)	missense	probably benign
R1462:Sec23ip	UTSW	7	128,367,862 (GRCm39)	missense	probably benign
R1564:Sec23ip	UTSW	7	128,368,005 (GRCm39)	splice site	probably null
R1876:Sec23ip	UTSW	7	128,354,575 (GRCm39)	missense	probably benign
R1966:Sec23ip	UTSW	7	128,357,077 (GRCm39)	missense	probably damaging	0.98
R1977:Sec23ip	UTSW	7	128,367,997 (GRCm39)	missense	probably damaging	1.00
R2115:Sec23ip	UTSW	7	128,364,185 (GRCm39)	missense	probably benign	0.00
R2847:Sec23ip	UTSW	7	128,355,797 (GRCm39)	missense	probably benign	0.00
R3958:Sec23ip	UTSW	7	128,378,574 (GRCm39)	missense	probably benign	0.35
R3959:Sec23ip	UTSW	7	128,378,574 (GRCm39)	missense	probably benign	0.35
R3960:Sec23ip	UTSW	7	128,378,574 (GRCm39)	missense	probably benign	0.35
R4287:Sec23ip	UTSW	7	128,379,057 (GRCm39)	missense	probably benign	0.37
R4510:Sec23ip	UTSW	7	128,380,900 (GRCm39)	missense	probably damaging	1.00
R4511:Sec23ip	UTSW	7	128,380,900 (GRCm39)	missense	probably damaging	1.00
R4612:Sec23ip	UTSW	7	128,352,226 (GRCm39)	nonsense	probably null
R4660:Sec23ip	UTSW	7	128,352,010 (GRCm39)	missense	probably null	0.00
R4890:Sec23ip	UTSW	7	128,354,634 (GRCm39)	missense	probably damaging	0.98
R5287:Sec23ip	UTSW	7	128,367,860 (GRCm39)	missense	probably benign
R5587:Sec23ip	UTSW	7	128,352,151 (GRCm39)	missense	probably benign
R5625:Sec23ip	UTSW	7	128,346,707 (GRCm39)	unclassified	probably benign
R5656:Sec23ip	UTSW	7	128,378,508 (GRCm39)	missense	probably damaging	1.00
R5808:Sec23ip	UTSW	7	128,373,908 (GRCm39)	missense	probably benign	0.00
R6034:Sec23ip	UTSW	7	128,351,927 (GRCm39)	missense	possibly damaging	0.66
R6034:Sec23ip	UTSW	7	128,351,927 (GRCm39)	missense	possibly damaging	0.66
R6145:Sec23ip	UTSW	7	128,380,208 (GRCm39)	missense	probably damaging	0.99
R6953:Sec23ip	UTSW	7	128,354,520 (GRCm39)	nonsense	probably null
R6992:Sec23ip	UTSW	7	128,367,164 (GRCm39)	missense	probably benign
R7131:Sec23ip	UTSW	7	128,381,364 (GRCm39)	missense	probably damaging	1.00
R7163:Sec23ip	UTSW	7	128,364,257 (GRCm39)	critical splice donor site	probably null
R7387:Sec23ip	UTSW	7	128,346,727 (GRCm39)	unclassified	probably benign
R7559:Sec23ip	UTSW	7	128,379,074 (GRCm39)	missense	possibly damaging	0.65
R7975:Sec23ip	UTSW	7	128,364,201 (GRCm39)	missense	probably damaging	1.00
R8158:Sec23ip	UTSW	7	128,369,364 (GRCm39)	missense	probably damaging	0.99
R8337:Sec23ip	UTSW	7	128,365,749 (GRCm39)	missense	probably damaging	1.00
R8409:Sec23ip	UTSW	7	128,365,855 (GRCm39)	missense	probably damaging	1.00
R8418:Sec23ip	UTSW	7	128,380,187 (GRCm39)	missense	probably damaging	0.98
R8434:Sec23ip	UTSW	7	128,352,151 (GRCm39)	missense	probably benign
R8461:Sec23ip	UTSW	7	128,373,926 (GRCm39)	missense	probably benign
R8553:Sec23ip	UTSW	7	128,355,777 (GRCm39)	missense	probably damaging	1.00
R8897:Sec23ip	UTSW	7	128,354,467 (GRCm39)	missense	probably benign	0.14
R9059:Sec23ip	UTSW	7	128,365,805 (GRCm39)	missense	probably damaging	1.00
R9142:Sec23ip	UTSW	7	128,363,226 (GRCm39)	missense	probably damaging	1.00
R9674:Sec23ip	UTSW	7	128,380,187 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CACGTAGGATAGCTAATACTGATACC -3'
(R):5'- AGGATCTGAAAACCTGCACAG -3'

Sequencing Primer
(F):5'- GTGGTTACAGAATTTCTCTTGGACAC -3'
(R):5'- TCTGAAAACCTGCACAGATTAAGG -3'

Posted On

2019-03-08