Mutagenetix > Incidental Mutation

Incidental Mutation 'R7304:Unc79'

567184

Institutional Source

Beutler Lab

Gene Symbol

Unc79

Ensembl Gene

ENSMUSG00000021198

Gene Name

unc-79 homolog

Synonyms

9030205A07Rik, Mlca3

MMRRC Submission

045365-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7304 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

102915118-103150324 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 103029449 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 484 (T484M)

Ref Sequence

ENSEMBL: ENSMUSP00000136332 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085079] [ENSMUST00000101099] [ENSMUST00000178001] [ENSMUST00000178076] [ENSMUST00000179002]

AlphaFold

Q0KK59

Predicted Effect

probably damaging
Transcript: ENSMUST00000085079
AA Change: T360M

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000082156
Gene: ENSMUSG00000021198
AA Change: T360M

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	469	3.1e-223	PFAM
low complexity region	732	737	N/A	INTRINSIC
low complexity region	846	862	N/A	INTRINSIC
low complexity region	968	977	N/A	INTRINSIC
low complexity region	1114	1125	N/A	INTRINSIC
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1428	1440	N/A	INTRINSIC
low complexity region	1471	1476	N/A	INTRINSIC
low complexity region	1477	1489	N/A	INTRINSIC
low complexity region	1490	1504	N/A	INTRINSIC
low complexity region	1541	1556	N/A	INTRINSIC
low complexity region	1861	1870	N/A	INTRINSIC
low complexity region	2237	2246	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101099
AA Change: T537M

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098659
Gene: ENSMUSG00000021198
AA Change: T537M

Domain	Start	End	E-Value	Type
Pfam:UNC-79	113	646	1.2e-226	PFAM
low complexity region	909	914	N/A	INTRINSIC
low complexity region	1023	1039	N/A	INTRINSIC
low complexity region	1145	1154	N/A	INTRINSIC
low complexity region	1291	1302	N/A	INTRINSIC
low complexity region	1490	1502	N/A	INTRINSIC
low complexity region	1605	1617	N/A	INTRINSIC
low complexity region	1648	1653	N/A	INTRINSIC
low complexity region	1654	1666	N/A	INTRINSIC
low complexity region	1667	1681	N/A	INTRINSIC
low complexity region	1718	1733	N/A	INTRINSIC
low complexity region	1999	2008	N/A	INTRINSIC
low complexity region	2375	2384	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000178001

SMART Domains

Protein: ENSMUSP00000137132
Gene: ENSMUSG00000021198

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	80	1.2e-30	PFAM
Pfam:UNC-79	78	188	2.1e-53	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000178076
AA Change: T341M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000136888
Gene: ENSMUSG00000021198
AA Change: T341M

Domain	Start	End	E-Value	Type
Pfam:UNC-79	1	450	4.2e-213	PFAM
low complexity region	713	718	N/A	INTRINSIC
low complexity region	827	843	N/A	INTRINSIC
low complexity region	949	958	N/A	INTRINSIC
low complexity region	1117	1128	N/A	INTRINSIC
low complexity region	1316	1328	N/A	INTRINSIC
low complexity region	1431	1443	N/A	INTRINSIC
low complexity region	1474	1479	N/A	INTRINSIC
low complexity region	1480	1492	N/A	INTRINSIC
low complexity region	1493	1507	N/A	INTRINSIC
low complexity region	1544	1559	N/A	INTRINSIC
low complexity region	1864	1873	N/A	INTRINSIC
low complexity region	2240	2249	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000179002
AA Change: T484M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000136332
Gene: ENSMUSG00000021198
AA Change: T484M

Domain	Start	End	E-Value	Type
Pfam:UNC-79	60	593	1.3e-226	PFAM
low complexity region	856	861	N/A	INTRINSIC
low complexity region	970	986	N/A	INTRINSIC
low complexity region	1092	1101	N/A	INTRINSIC
low complexity region	1260	1271	N/A	INTRINSIC
low complexity region	1509	1521	N/A	INTRINSIC
low complexity region	1624	1636	N/A	INTRINSIC
low complexity region	1667	1672	N/A	INTRINSIC
low complexity region	1673	1685	N/A	INTRINSIC
low complexity region	1686	1700	N/A	INTRINSIC
low complexity region	1737	1752	N/A	INTRINSIC
low complexity region	2057	2066	N/A	INTRINSIC
low complexity region	2433	2442	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The NALCN channel is responsible for Na(+) leak currents. The protein encoded by this gene, along with UNC80, is an accessory subunit of the NALCN channel that contributes to the Ca(2+) sensitivity of the channel. [provided by RefSeq, Sep 2016]
PHENOTYPE: Homozygous mutation results in lethality within the first week after birth, mostly at P0 or P1. Pups fail to nurse and have no milk in stomachs resulting in weakness, inactivity and no weight gain. [provided by MGI curators]

Allele List at MGI

All alleles(2) : Targeted, knock-out(1) Chemically induced(1)

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	G	11: 9,247,203 (GRCm39)	S2317G	probably benign	Het
Acap2	A	C	16: 30,926,934 (GRCm39)	L502R	probably benign	Het
Amotl2	A	G	9: 102,605,549 (GRCm39)	E467G	probably damaging	Het
Apbb1ip	A	T	2: 22,743,147 (GRCm39)		probably null	Het
Armc9	C	G	1: 86,090,437 (GRCm39)	D77E	probably benign	Het
Art1	T	A	7: 101,755,531 (GRCm39)	S8T	possibly damaging	Het
Asic5	G	A	3: 81,916,872 (GRCm39)	A321T	possibly damaging	Het
Astn2	A	G	4: 66,103,612 (GRCm39)	I267T	unknown	Het
Bmp8a	T	C	4: 123,236,182 (GRCm39)	N107S	probably benign	Het
Card14	T	A	11: 119,228,573 (GRCm39)	L633Q	probably damaging	Het
Chpf	C	T	1: 75,455,698 (GRCm39)	V18M	possibly damaging	Het
Cog7	T	C	7: 121,536,362 (GRCm39)	I493V	probably benign	Het
Col3a1	A	T	1: 45,386,971 (GRCm39)	N1394I	unknown	Het
Crybg1	C	T	10: 43,873,254 (GRCm39)	D1285N	probably benign	Het
Depdc7	A	G	2: 104,553,463 (GRCm39)	V395A	possibly damaging	Het
Dido1	G	A	2: 180,329,286 (GRCm39)	L379F	probably damaging	Het
Dnajc13	G	T	9: 104,115,713 (GRCm39)	T32N	probably benign	Het
Dok2	T	A	14: 71,013,468 (GRCm39)	S133R	probably benign	Het
Ehbp1l1	C	T	19: 5,766,410 (GRCm39)	R1311H	probably damaging	Het
Gm3099	A	T	14: 15,346,488 (GRCm39)	N118I	probably damaging	Het
Gtse1	A	G	15: 85,755,748 (GRCm39)	T471A	probably benign	Het
Heg1	G	A	16: 33,581,160 (GRCm39)	A13T	possibly damaging	Het
Ifi209	A	T	1: 173,470,156 (GRCm39)	Y248F	possibly damaging	Het
Irag1	T	C	7: 110,498,931 (GRCm39)	T367A	possibly damaging	Het
Itgb3bp	C	G	4: 99,657,758 (GRCm39)	E169Q	probably damaging	Het
Kcna2	A	G	3: 107,012,066 (GRCm39)	T216A	probably benign	Het
Kcnj6	A	T	16: 94,742,042 (GRCm39)	M10K	probably benign	Het
Krt17	T	G	11: 100,148,163 (GRCm39)	Q397P	probably benign	Het
Lrtm1	T	A	14: 28,744,075 (GRCm39)	M181K	probably damaging	Het
Map3k5	A	G	10: 19,975,301 (GRCm39)	H741R	probably damaging	Het
Mier1	T	A	4: 102,996,599 (GRCm39)	Y120*	probably null	Het
Myh14	T	A	7: 44,279,415 (GRCm39)	T922S	probably benign	Het
Nfkbie	A	T	17: 45,871,067 (GRCm39)	I240F	possibly damaging	Het
Npas3	C	A	12: 54,115,824 (GRCm39)	H915Q	probably damaging	Het
Nrg2	A	T	18: 36,178,994 (GRCm39)	V314E	probably benign	Het
Or2z9	T	A	8: 72,854,190 (GRCm39)	Y195*	probably null	Het
Pds5a	T	C	5: 65,777,077 (GRCm39)	N28S	probably damaging	Het
Pira1	T	C	7: 3,740,493 (GRCm39)	T243A	probably damaging	Het
Pkhd1l1	A	T	15: 44,361,878 (GRCm39)	N517I	possibly damaging	Het
Plekhb1	T	C	7: 100,294,874 (GRCm39)	Y99C	probably benign	Het
Plpp6	T	C	19: 28,941,617 (GRCm39)	S73P	probably benign	Het
Polb	T	C	8: 23,129,975 (GRCm39)	N199S	probably benign	Het
Ppp1r13b	T	C	12: 111,838,840 (GRCm39)	N13D	possibly damaging	Het
Prorp	A	G	12: 55,351,429 (GRCm39)	D246G	probably damaging	Het
Ptprn2	T	A	12: 117,212,164 (GRCm39)	V862D	probably damaging	Het
Rassf4	T	C	6: 116,617,278 (GRCm39)	I242M	probably damaging	Het
Rnf19a	G	A	15: 36,254,598 (GRCm39)	T320I	probably damaging	Het
Sctr	A	T	1: 119,949,970 (GRCm39)	E53V	probably damaging	Het
Srcin1	T	C	11: 97,442,519 (GRCm39)	D103G	probably benign	Het
St3gal3	T	C	4: 117,814,633 (GRCm39)	Q220R		Het
Stk40	G	A	4: 126,019,483 (GRCm39)	E86K	probably benign	Het
Tas2r104	T	A	6: 131,662,005 (GRCm39)	I235F	possibly damaging	Het
Terf2ip	T	C	8: 112,738,280 (GRCm39)	V56A	possibly damaging	Het
Thsd7b	A	G	1: 130,030,890 (GRCm39)	N1075S	probably benign	Het
Trak1	A	G	9: 121,245,278 (GRCm39)	Y51C	probably benign	Het
Trav16n	T	A	14: 53,588,859 (GRCm39)	V45E	probably benign	Het
Ttc6	T	C	12: 57,622,837 (GRCm39)	S79P	probably damaging	Het
Usp49	T	C	17: 47,983,796 (GRCm39)	V267A	possibly damaging	Het
Vldlr	T	A	19: 27,216,004 (GRCm39)	D305E	possibly damaging	Het
Vmn1r226	G	T	17: 20,908,011 (GRCm39)	C81F	probably damaging	Het
Vmn1r73	T	C	7: 11,490,824 (GRCm39)	V214A	probably damaging	Het
Vmn2r5	T	C	3: 64,411,671 (GRCm39)	N299S	probably damaging	Het
Vwa3b	T	C	1: 37,203,586 (GRCm39)	L55S	probably damaging	Het
Wdr90	T	C	17: 26,070,480 (GRCm39)	D1105G	probably benign	Het
Zbtb38	A	G	9: 96,569,480 (GRCm39)	S535P	probably damaging	Het
Zfp329	T	C	7: 12,544,826 (GRCm39)	I233V	probably damaging	Het
Zfp579	T	A	7: 4,997,582 (GRCm39)	T110S	probably benign	Het

Other mutations in Unc79

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00718:Unc79	APN	12	103,135,906 (GRCm39)	missense	possibly damaging	0.68
IGL00835:Unc79	APN	12	103,108,149 (GRCm39)	splice site	probably benign
IGL00917:Unc79	APN	12	103,054,766 (GRCm39)	missense	possibly damaging	0.53
IGL01012:Unc79	APN	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
IGL01121:Unc79	APN	12	103,131,890 (GRCm39)	missense	probably damaging	0.99
IGL01303:Unc79	APN	12	103,128,126 (GRCm39)	missense	possibly damaging	0.94
IGL01305:Unc79	APN	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
IGL01315:Unc79	APN	12	103,054,780 (GRCm39)	missense	possibly damaging	0.66
IGL01388:Unc79	APN	12	103,136,018 (GRCm39)	splice site	probably benign
IGL01415:Unc79	APN	12	103,074,944 (GRCm39)	missense	probably damaging	1.00
IGL01447:Unc79	APN	12	103,045,177 (GRCm39)	missense	probably damaging	1.00
IGL01655:Unc79	APN	12	103,134,546 (GRCm39)	missense	probably benign	0.00
IGL01662:Unc79	APN	12	103,115,279 (GRCm39)	missense	possibly damaging	0.92
IGL01728:Unc79	APN	12	103,131,943 (GRCm39)	missense	probably damaging	0.98
IGL01767:Unc79	APN	12	103,108,256 (GRCm39)	missense	probably damaging	1.00
IGL02080:Unc79	APN	12	102,968,234 (GRCm39)	missense	probably damaging	1.00
IGL02115:Unc79	APN	12	102,964,933 (GRCm39)	missense	probably damaging	1.00
IGL02176:Unc79	APN	12	102,965,006 (GRCm39)	splice site	probably null
IGL02186:Unc79	APN	12	102,977,542 (GRCm39)	missense	probably benign	0.04
IGL02205:Unc79	APN	12	103,045,260 (GRCm39)	missense	probably damaging	1.00
IGL02337:Unc79	APN	12	103,122,705 (GRCm39)	splice site	probably benign
IGL02498:Unc79	APN	12	103,137,837 (GRCm39)	missense	probably damaging	0.99
IGL02508:Unc79	APN	12	103,078,535 (GRCm39)	missense	probably damaging	0.97
IGL02508:Unc79	APN	12	103,078,277 (GRCm39)	splice site	probably benign
IGL02557:Unc79	APN	12	103,148,418 (GRCm39)	splice site	probably benign
IGL02589:Unc79	APN	12	103,139,755 (GRCm39)	missense	probably damaging	1.00
IGL02611:Unc79	APN	12	103,131,967 (GRCm39)	missense	probably damaging	0.97
IGL02728:Unc79	APN	12	103,088,688 (GRCm39)	missense	possibly damaging	0.53
IGL02827:Unc79	APN	12	103,041,105 (GRCm39)	missense	possibly damaging	0.88
IGL03028:Unc79	APN	12	103,139,785 (GRCm39)	missense	possibly damaging	0.83
IGL03144:Unc79	APN	12	103,008,401 (GRCm39)	missense	probably damaging	1.00
IGL03229:Unc79	APN	12	103,100,798 (GRCm39)	missense	probably damaging	0.99
IGL03269:Unc79	APN	12	103,054,936 (GRCm39)	missense	probably damaging	1.00
IGL03325:Unc79	APN	12	103,135,869 (GRCm39)	missense	probably damaging	0.98
pencil-thin	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
sweetpea	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
3-1:Unc79	UTSW	12	103,039,009 (GRCm39)	nonsense	probably null
ANU22:Unc79	UTSW	12	102,968,130 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0046:Unc79	UTSW	12	103,091,940 (GRCm39)	missense	probably damaging	0.99
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0067:Unc79	UTSW	12	103,025,777 (GRCm39)	missense	probably damaging	1.00
R0107:Unc79	UTSW	12	103,100,784 (GRCm39)	missense	possibly damaging	0.70
R0110:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0128:Unc79	UTSW	12	103,054,693 (GRCm39)	splice site	probably benign
R0166:Unc79	UTSW	12	103,122,812 (GRCm39)	missense	probably damaging	1.00
R0208:Unc79	UTSW	12	103,058,286 (GRCm39)	missense	probably benign	0.00
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0211:Unc79	UTSW	12	103,039,051 (GRCm39)	missense	probably benign	0.01
R0218:Unc79	UTSW	12	103,075,040 (GRCm39)	splice site	probably null
R0244:Unc79	UTSW	12	103,079,150 (GRCm39)	missense	probably damaging	1.00
R0305:Unc79	UTSW	12	103,079,459 (GRCm39)	missense	probably benign	0.18
R0310:Unc79	UTSW	12	103,027,666 (GRCm39)	missense	probably damaging	1.00
R0325:Unc79	UTSW	12	103,137,903 (GRCm39)	missense	probably damaging	0.98
R0369:Unc79	UTSW	12	103,055,031 (GRCm39)	critical splice donor site	probably null
R0450:Unc79	UTSW	12	103,045,329 (GRCm39)	critical splice donor site	probably null
R0503:Unc79	UTSW	12	103,045,127 (GRCm39)	missense	probably benign	0.01
R0542:Unc79	UTSW	12	103,060,437 (GRCm39)	splice site	probably benign
R0845:Unc79	UTSW	12	103,139,703 (GRCm39)	splice site	probably benign
R0893:Unc79	UTSW	12	102,957,687 (GRCm39)	missense	probably damaging	1.00
R1078:Unc79	UTSW	12	103,041,112 (GRCm39)	missense	probably benign	0.03
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1148:Unc79	UTSW	12	103,078,926 (GRCm39)	missense	probably damaging	1.00
R1159:Unc79	UTSW	12	103,013,311 (GRCm39)	splice site	probably benign
R1191:Unc79	UTSW	12	103,013,271 (GRCm39)	nonsense	probably null
R1307:Unc79	UTSW	12	103,036,335 (GRCm39)	missense	probably damaging	1.00
R1368:Unc79	UTSW	12	103,122,772 (GRCm39)	missense	probably damaging	1.00
R1476:Unc79	UTSW	12	103,149,784 (GRCm39)	missense	probably damaging	1.00
R1650:Unc79	UTSW	12	103,079,052 (GRCm39)	missense	possibly damaging	0.85
R1777:Unc79	UTSW	12	103,078,714 (GRCm39)	missense	probably damaging	1.00
R1796:Unc79	UTSW	12	103,109,005 (GRCm39)	missense	probably damaging	0.99
R1824:Unc79	UTSW	12	103,025,579 (GRCm39)	missense	probably damaging	1.00
R1830:Unc79	UTSW	12	103,100,737 (GRCm39)	missense	probably damaging	1.00
R1927:Unc79	UTSW	12	103,135,951 (GRCm39)	missense	probably damaging	1.00
R1958:Unc79	UTSW	12	103,041,178 (GRCm39)	missense	probably benign	0.19
R1958:Unc79	UTSW	12	102,957,621 (GRCm39)	missense	probably damaging	1.00
R1980:Unc79	UTSW	12	102,977,538 (GRCm39)	nonsense	probably null
R2019:Unc79	UTSW	12	103,137,830 (GRCm39)	critical splice acceptor site	probably null
R2290:Unc79	UTSW	12	103,112,625 (GRCm39)	missense	probably damaging	1.00
R2939:Unc79	UTSW	12	102,957,684 (GRCm39)	missense	probably damaging	1.00
R2962:Unc79	UTSW	12	103,061,378 (GRCm39)	missense	possibly damaging	0.72
R3176:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3276:Unc79	UTSW	12	103,079,476 (GRCm39)	missense	probably damaging	1.00
R3683:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3684:Unc79	UTSW	12	103,041,062 (GRCm39)	missense	probably benign	0.00
R3686:Unc79	UTSW	12	103,054,920 (GRCm39)	missense	probably damaging	1.00
R3760:Unc79	UTSW	12	103,058,964 (GRCm39)	missense	probably damaging	1.00
R4031:Unc79	UTSW	12	103,039,018 (GRCm39)	missense	possibly damaging	0.46
R4039:Unc79	UTSW	12	103,041,208 (GRCm39)	missense	possibly damaging	0.88
R4110:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4113:Unc79	UTSW	12	103,025,629 (GRCm39)	missense	probably damaging	1.00
R4159:Unc79	UTSW	12	103,036,512 (GRCm39)	intron	probably benign
R4273:Unc79	UTSW	12	103,088,612 (GRCm39)	missense	probably damaging	0.99
R4292:Unc79	UTSW	12	103,149,703 (GRCm39)	missense	probably damaging	0.99
R4334:Unc79	UTSW	12	103,045,233 (GRCm39)	missense	probably benign
R4513:Unc79	UTSW	12	102,988,019 (GRCm39)	missense	probably damaging	1.00
R4562:Unc79	UTSW	12	102,957,720 (GRCm39)	missense	probably damaging	1.00
R4576:Unc79	UTSW	12	102,968,062 (GRCm39)	splice site	probably benign
R4645:Unc79	UTSW	12	103,079,081 (GRCm39)	missense	probably benign
R4758:Unc79	UTSW	12	103,128,080 (GRCm39)	nonsense	probably null
R4787:Unc79	UTSW	12	103,013,257 (GRCm39)	missense	probably damaging	1.00
R4852:Unc79	UTSW	12	103,139,725 (GRCm39)	missense	probably damaging	0.98
R4883:Unc79	UTSW	12	103,060,592 (GRCm39)	missense	probably damaging	0.99
R4898:Unc79	UTSW	12	103,128,079 (GRCm39)	missense	probably damaging	0.99
R4979:Unc79	UTSW	12	103,078,691 (GRCm39)	missense	probably benign
R5044:Unc79	UTSW	12	103,078,962 (GRCm39)	missense	probably benign	0.32
R5053:Unc79	UTSW	12	103,071,007 (GRCm39)	missense	probably damaging	1.00
R5061:Unc79	UTSW	12	103,134,700 (GRCm39)	missense	possibly damaging	0.94
R5075:Unc79	UTSW	12	103,041,213 (GRCm39)	missense	possibly damaging	0.63
R5101:Unc79	UTSW	12	103,078,769 (GRCm39)	missense	probably damaging	1.00
R5236:Unc79	UTSW	12	103,060,654 (GRCm39)	critical splice donor site	probably null
R5240:Unc79	UTSW	12	103,037,010 (GRCm39)	missense	probably damaging	0.99
R5383:Unc79	UTSW	12	103,070,886 (GRCm39)	missense	possibly damaging	0.53
R5461:Unc79	UTSW	12	103,078,397 (GRCm39)	missense	probably damaging	1.00
R5535:Unc79	UTSW	12	103,135,962 (GRCm39)	missense	possibly damaging	0.84
R5609:Unc79	UTSW	12	103,094,527 (GRCm39)	missense	probably benign
R5639:Unc79	UTSW	12	103,137,831 (GRCm39)	missense	probably damaging	1.00
R5704:Unc79	UTSW	12	102,968,202 (GRCm39)	missense	probably damaging	1.00
R5923:Unc79	UTSW	12	103,078,727 (GRCm39)	missense	probably damaging	1.00
R5925:Unc79	UTSW	12	103,091,989 (GRCm39)	splice site	probably null
R5975:Unc79	UTSW	12	103,091,885 (GRCm39)	missense	possibly damaging	0.53
R6047:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6156:Unc79	UTSW	12	103,027,717 (GRCm39)	missense	probably damaging	1.00
R6175:Unc79	UTSW	12	103,149,708 (GRCm39)	missense	probably damaging	0.98
R6292:Unc79	UTSW	12	103,108,991 (GRCm39)	missense	possibly damaging	0.88
R6313:Unc79	UTSW	12	103,078,878 (GRCm39)	missense	probably damaging	1.00
R6391:Unc79	UTSW	12	102,987,269 (GRCm39)	missense	probably damaging	1.00
R6405:Unc79	UTSW	12	103,134,595 (GRCm39)	missense	probably damaging	0.97
R6416:Unc79	UTSW	12	103,097,905 (GRCm39)	missense	possibly damaging	0.86
R6467:Unc79	UTSW	12	103,139,771 (GRCm39)	missense	probably damaging	1.00
R6573:Unc79	UTSW	12	103,027,647 (GRCm39)	missense	probably damaging	1.00
R6614:Unc79	UTSW	12	102,957,689 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,308 (GRCm39)	missense	probably damaging	1.00
R6654:Unc79	UTSW	12	103,045,307 (GRCm39)	missense	probably damaging	0.99
R6700:Unc79	UTSW	12	103,091,962 (GRCm39)	missense	possibly damaging	0.92
R6724:Unc79	UTSW	12	103,071,120 (GRCm39)	missense	probably damaging	1.00
R6819:Unc79	UTSW	12	103,108,267 (GRCm39)	missense	probably benign	0.12
R6869:Unc79	UTSW	12	103,079,331 (GRCm39)	missense	probably benign	0.33
R6879:Unc79	UTSW	12	103,115,046 (GRCm39)	splice site	probably null
R6942:Unc79	UTSW	12	103,088,704 (GRCm39)	critical splice donor site	probably null
R6961:Unc79	UTSW	12	103,079,174 (GRCm39)	missense	probably damaging	1.00
R6973:Unc79	UTSW	12	102,964,699 (GRCm39)	missense	possibly damaging	0.86
R6980:Unc79	UTSW	12	103,025,759 (GRCm39)	missense	probably damaging	1.00
R7124:Unc79	UTSW	12	103,027,652 (GRCm39)	missense	probably damaging	0.99
R7144:Unc79	UTSW	12	103,108,885 (GRCm39)	missense	probably benign	0.06
R7197:Unc79	UTSW	12	103,078,765 (GRCm39)	missense	probably benign
R7209:Unc79	UTSW	12	103,091,883 (GRCm39)	missense	probably benign
R7232:Unc79	UTSW	12	103,100,734 (GRCm39)	missense	possibly damaging	0.49
R7354:Unc79	UTSW	12	103,108,961 (GRCm39)	missense	possibly damaging	0.79
R7384:Unc79	UTSW	12	103,137,837 (GRCm39)	missense	probably benign	0.11
R7400:Unc79	UTSW	12	103,070,889 (GRCm39)	missense	probably damaging	1.00
R7417:Unc79	UTSW	12	103,055,017 (GRCm39)	missense	possibly damaging	0.85
R7470:Unc79	UTSW	12	103,061,235 (GRCm39)	missense	probably damaging	1.00
R7842:Unc79	UTSW	12	103,058,313 (GRCm39)	missense	probably damaging	1.00
R8037:Unc79	UTSW	12	103,016,178 (GRCm39)	missense	probably damaging	1.00
R8041:Unc79	UTSW	12	103,054,726 (GRCm39)	missense	probably benign	0.06
R8146:Unc79	UTSW	12	103,036,416 (GRCm39)	missense	probably damaging	0.98
R8276:Unc79	UTSW	12	102,968,122 (GRCm39)	missense	possibly damaging	0.94
R8427:Unc79	UTSW	12	103,045,297 (GRCm39)	missense	probably benign	0.24
R8501:Unc79	UTSW	12	103,058,897 (GRCm39)	missense	probably damaging	1.00
R8510:Unc79	UTSW	12	103,070,898 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,013,922 (GRCm39)	missense	probably damaging	1.00
R8531:Unc79	UTSW	12	103,049,855 (GRCm39)	missense	probably benign	0.13
R8795:Unc79	UTSW	12	103,074,513 (GRCm39)	missense	probably damaging	1.00
R9017:Unc79	UTSW	12	103,074,874 (GRCm39)	critical splice acceptor site	probably null
R9121:Unc79	UTSW	12	102,968,095 (GRCm39)	missense	probably damaging	1.00
R9196:Unc79	UTSW	12	103,078,613 (GRCm39)	missense	probably benign
R9443:Unc79	UTSW	12	103,037,035 (GRCm39)	missense	probably damaging	1.00
R9548:Unc79	UTSW	12	102,977,495 (GRCm39)	missense	probably damaging	1.00
R9600:Unc79	UTSW	12	103,135,972 (GRCm39)	missense	probably benign	0.07
R9767:Unc79	UTSW	12	103,079,234 (GRCm39)	missense	probably benign
R9787:Unc79	UTSW	12	103,112,620 (GRCm39)	missense	probably benign	0.00
RF010:Unc79	UTSW	12	103,079,046 (GRCm39)	missense	probably benign	0.17
X0017:Unc79	UTSW	12	103,074,520 (GRCm39)	missense	probably damaging	0.99
X0028:Unc79	UTSW	12	102,957,662 (GRCm39)	missense	probably damaging	1.00
Z1088:Unc79	UTSW	12	102,987,271 (GRCm39)	missense	probably damaging	1.00
Z1176:Unc79	UTSW	12	103,108,312 (GRCm39)	missense	probably benign	0.03
Z1176:Unc79	UTSW	12	103,054,937 (GRCm39)	missense	probably damaging	1.00
Z1177:Unc79	UTSW	12	103,131,948 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GAATGCATCATCTGGAGCCC -3'
(R):5'- GTCAAATGTAGCATTGCCCC -3'

Sequencing Primer
(F):5'- GGAGCCCCTCCTTTTTCC -3'
(R):5'- CCCGCAGCAAGGCCTAGATAG -3'

Posted On

2019-06-26