Mutagenetix > Incidental Mutation

Incidental Mutation 'R2254:Glyr1'

241898

Institutional Source

Beutler Lab

Gene Symbol

Glyr1

Ensembl Gene

ENSMUSG00000022536

Gene Name

glyoxylate reductase 1 homolog (Arabidopsis)

Synonyms

NDF, 2810419J22Rik, 3930401K13Rik, Npac

MMRRC Submission

040254-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.433) question?

Stock #

R2254 (G1)

Quality Score

203

Status

Not validated

Chromosome

Chromosomal Location

4831773-4867727 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 4836877 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 429 (V429A)

Ref Sequence

ENSEMBL: ENSMUSP00000111510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023189] [ENSMUST00000115844]

AlphaFold

Q922P9

Predicted Effect

probably benign
Transcript: ENSMUST00000023189
AA Change: V423A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000023189
Gene: ENSMUSG00000022536
AA Change: V423A

Domain	Start	End	E-Value	Type
Pfam:PWWP	6	89	1.4e-24	PFAM
low complexity region	109	122	N/A	INTRINSIC
AT_hook	167	179	3.21e-1	SMART
Pfam:NAD_binding_2	266	422	1.2e-36	PFAM
Pfam:F420_oxidored	268	355	2e-6	PFAM
Pfam:NAD_binding_11	423	544	2.7e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000115844
AA Change: V429A

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000111510
Gene: ENSMUSG00000022536
AA Change: V429A

Domain	Start	End	E-Value	Type
Pfam:PWWP	6	89	1.8e-24	PFAM
low complexity region	109	122	N/A	INTRINSIC
AT_hook	167	179	3.21e-1	SMART
Pfam:NAD_binding_2	266	428	2.6e-41	PFAM
Pfam:F420_oxidored	268	361	3.2e-9	PFAM
Pfam:NAD_binding_11	429	550	3.4e-25	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn2	T	C	13: 12,311,365 (GRCm39)	E260G	probably benign	Het
AI429214	G	T	8: 37,460,920 (GRCm39)	D23Y	possibly damaging	Het
Alms1	A	G	6: 85,596,830 (GRCm39)	Y1021C	probably damaging	Het
Ang5	A	G	14: 44,200,074 (GRCm39)	D46G	probably benign	Het
Ano9	T	A	7: 140,683,003 (GRCm39)	D635V	probably benign	Het
Apob	C	T	12: 8,061,256 (GRCm39)	T3246I	possibly damaging	Het
Arhgef25	T	C	10: 127,025,390 (GRCm39)	E63G	probably benign	Het
B3gnt4	A	C	5: 123,649,342 (GRCm39)	I236L	probably damaging	Het
Bmper	T	A	9: 23,292,759 (GRCm39)	I356N	possibly damaging	Het
Capn3	A	G	2: 120,331,732 (GRCm39)	E614G	probably benign	Het
Ccdc90b	T	A	7: 92,221,776 (GRCm39)	H118Q	probably damaging	Het
Cdcp3	G	T	7: 130,824,634 (GRCm39)	C243F	probably damaging	Het
Cdh2	A	T	18: 16,776,985 (GRCm39)		probably null	Het
Chmp7	C	T	14: 69,958,405 (GRCm39)	V255I	probably damaging	Het
Dnhd1	T	A	7: 105,352,979 (GRCm39)	S2711T	probably damaging	Het
Gabrg1	T	A	5: 70,939,707 (GRCm39)	K137*	probably null	Het
Gdi2	T	A	13: 3,604,400 (GRCm39)		probably null	Het
Gm5938	T	A	X: 77,172,161 (GRCm39)		probably null	Het
Golt1b	T	C	6: 142,341,979 (GRCm39)	L121P	probably damaging	Het
Gpi1	T	C	7: 33,902,302 (GRCm39)	N471S	probably damaging	Het
Ifi204	G	A	1: 173,589,296 (GRCm39)	T45M	possibly damaging	Het
Il18r1	A	G	1: 40,530,380 (GRCm39)	N369S	possibly damaging	Het
Kcnh1	G	T	1: 192,187,722 (GRCm39)		probably null	Het
Kitl	G	A	10: 99,915,993 (GRCm39)		probably null	Het
Krt33a	A	T	11: 99,905,004 (GRCm39)	D167E	possibly damaging	Het
Lax1	A	G	1: 133,607,971 (GRCm39)	S257P	probably damaging	Het
Lepr	T	C	4: 101,672,309 (GRCm39)	I1111T	probably benign	Het
Lrrcc1	G	A	3: 14,612,315 (GRCm39)	R356H	probably damaging	Het
Map1a	A	G	2: 121,134,272 (GRCm39)	D1458G	possibly damaging	Het
Med11	T	C	11: 70,342,921 (GRCm39)		probably null	Het
Mtmr2	T	C	9: 13,707,353 (GRCm39)	Y230H	possibly damaging	Het
Nup93	T	C	8: 94,954,485 (GRCm39)		probably null	Het
Or51ag1	A	G	7: 103,155,271 (GRCm39)	V294A	probably damaging	Het
Ovch2	A	G	7: 107,389,402 (GRCm39)	V342A	probably benign	Het
Ovgp1	A	G	3: 105,894,228 (GRCm39)		probably benign	Het
Oxtr	A	T	6: 112,466,067 (GRCm39)	L231Q	probably damaging	Het
Prap1	A	G	7: 139,676,075 (GRCm39)	T30A	probably damaging	Het
Scg2	G	A	1: 79,414,217 (GRCm39)	P169S	probably damaging	Het
Scube2	A	G	7: 109,424,666 (GRCm39)	V549A	possibly damaging	Het
Slc22a12	A	T	19: 6,592,571 (GRCm39)	V57D	possibly damaging	Het
Slc22a28	A	C	19: 8,041,858 (GRCm39)	C450G	probably benign	Het
Tas2r120	A	T	6: 132,634,572 (GRCm39)	Q218L	probably benign	Het
Tbx15	A	G	3: 99,259,190 (GRCm39)	T354A	possibly damaging	Het
Trim24	A	G	6: 37,935,612 (GRCm39)	T868A	probably benign	Het
Ttn	T	A	2: 76,598,684 (GRCm39)	M19410L	possibly damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Upb1	G	A	10: 75,272,051 (GRCm39)	R288H	probably damaging	Het
Wdr20rt	T	A	12: 65,273,007 (GRCm39)	W56R	probably damaging	Het
Wdr62	T	G	7: 29,967,328 (GRCm39)	I309L	probably damaging	Het
Zer1	G	A	2: 29,998,286 (GRCm39)	L342F	probably damaging	Het
Zfp40	T	C	17: 23,397,344 (GRCm39)	D51G	possibly damaging	Het
Zfp64	G	A	2: 168,768,662 (GRCm39)	H317Y	probably damaging	Het

Other mutations in Glyr1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01369:Glyr1	APN	16	4,838,152 (GRCm39)	missense	probably benign
IGL02332:Glyr1	APN	16	4,836,817 (GRCm39)	missense	probably damaging	1.00
IGL02407:Glyr1	APN	16	4,854,812 (GRCm39)	missense	probably benign	0.01
IGL02429:Glyr1	APN	16	4,837,240 (GRCm39)	missense	probably benign	0.28
IGL03255:Glyr1	APN	16	4,866,621 (GRCm39)	splice site	probably null
R0020:Glyr1	UTSW	16	4,854,913 (GRCm39)	missense	probably damaging	1.00
R0309:Glyr1	UTSW	16	4,849,836 (GRCm39)	missense	probably damaging	0.98
R0694:Glyr1	UTSW	16	4,844,424 (GRCm39)	missense	probably damaging	0.99
R0971:Glyr1	UTSW	16	4,839,209 (GRCm39)	frame shift	probably null
R1347:Glyr1	UTSW	16	4,839,203 (GRCm39)	missense	probably damaging	1.00
R1347:Glyr1	UTSW	16	4,839,203 (GRCm39)	missense	probably damaging	1.00
R1382:Glyr1	UTSW	16	4,839,209 (GRCm39)	frame shift	probably null
R1383:Glyr1	UTSW	16	4,839,209 (GRCm39)	frame shift	probably null
R2137:Glyr1	UTSW	16	4,836,346 (GRCm39)	missense	probably benign	0.01
R3913:Glyr1	UTSW	16	4,849,777 (GRCm39)	missense	probably damaging	1.00
R4112:Glyr1	UTSW	16	4,836,350 (GRCm39)	missense	possibly damaging	0.83
R4629:Glyr1	UTSW	16	4,854,907 (GRCm39)	missense	possibly damaging	0.70
R4795:Glyr1	UTSW	16	4,865,622 (GRCm39)	missense	probably benign	0.40
R5112:Glyr1	UTSW	16	4,836,740 (GRCm39)	nonsense	probably null
R5412:Glyr1	UTSW	16	4,854,297 (GRCm39)	missense	possibly damaging	0.87
R5757:Glyr1	UTSW	16	4,836,856 (GRCm39)	missense	probably benign	0.30
R6425:Glyr1	UTSW	16	4,854,350 (GRCm39)	splice site	probably null
R7646:Glyr1	UTSW	16	4,836,361 (GRCm39)	missense	probably damaging	1.00
R7942:Glyr1	UTSW	16	4,836,785 (GRCm39)	missense	probably benign	0.43
R8415:Glyr1	UTSW	16	4,854,329 (GRCm39)	missense	probably benign	0.35
Z1177:Glyr1	UTSW	16	4,849,837 (GRCm39)	missense	probably null	1.00

Predicted Primers

PCR Primer
(F):5'- CTTTGGCACTTCTGGTCCAG -3'
(R):5'- GGACTAAAGGCTTGCAGGTG -3'

Sequencing Primer
(F):5'- GAAAGATGCTGGCCAATTGTCCC -3'
(R):5'- ATTGGTGACAGTGTCAGTCCCAC -3'

Posted On

2014-10-16