Mutagenetix > Incidental Mutation

Incidental Mutation 'R3077:Zfp647'

265267

Institutional Source

Beutler Lab

Gene Symbol

Zfp647

Ensembl Gene

ENSMUSG00000054967

Gene Name

zinc finger protein 647

Synonyms

MMRRC Submission

040567-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R3077 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

76794571-76809648 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 76802209 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000155660 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048854] [ENSMUST00000229055] [ENSMUST00000229865] [ENSMUST00000230677]

AlphaFold

Q7TNU6

Predicted Effect

probably null
Transcript: ENSMUST00000048854
AA Change: M1K

SMART Domains

Protein: ENSMUSP00000041575
Gene: ENSMUSG00000054967
AA Change: M1K

Domain	Start	End	E-Value	Type
low complexity region	2	15	N/A	INTRINSIC
KRAB	17	77	7.05e-33	SMART
ZnF_C2H2	174	196	3.39e-3	SMART
ZnF_C2H2	202	224	1.2e-3	SMART
ZnF_C2H2	230	252	2.95e-3	SMART
ZnF_C2H2	258	280	4.79e-3	SMART
ZnF_C2H2	286	308	1.84e-4	SMART
ZnF_C2H2	314	336	6.32e-3	SMART
ZnF_C2H2	342	364	7.37e-4	SMART
ZnF_C2H2	370	392	1.6e-4	SMART
ZnF_C2H2	398	420	2.2e-2	SMART
ZnF_C2H2	426	448	6.78e-3	SMART
ZnF_C2H2	454	476	4.87e-4	SMART
ZnF_C2H2	482	504	2.24e-3	SMART
ZnF_C2H2	510	532	9.08e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000229055
AA Change: M1K

Predicted Effect

probably null
Transcript: ENSMUST00000229865
AA Change: M1K

Predicted Effect

probably null
Transcript: ENSMUST00000230677
AA Change: M1K

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.5%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,306,764 (GRCm39)	I1981V	probably benign	Het
Adgrd1	A	T	5: 129,206,169 (GRCm39)	I248F	probably benign	Het
Arfgef3	T	C	10: 18,479,278 (GRCm39)	I1446V	probably damaging	Het
Cabcoco1	T	G	10: 68,361,475 (GRCm39)	Y8S	possibly damaging	Het
Champ1	T	C	8: 13,928,832 (GRCm39)	V330A	probably benign	Het
Dnajc8	A	G	4: 132,271,974 (GRCm39)	D70G	probably damaging	Het
Kif14	T	G	1: 136,447,383 (GRCm39)	I1396S	possibly damaging	Het
Mnt	G	C	11: 74,733,936 (GRCm39)		probably benign	Het
Nhsl2	C	T	X: 101,121,201 (GRCm39)	R62W	probably damaging	Het
Or1e29	G	A	11: 73,667,466 (GRCm39)	P229L	possibly damaging	Het
Or51a7	G	T	7: 102,615,223 (GRCm39)	K305N	probably benign	Het
Pcdhb11	C	T	18: 37,555,297 (GRCm39)	T209I	probably benign	Het
Pdzd8	A	G	19: 59,293,588 (GRCm39)		probably null	Het
Phactr4	A	G	4: 132,125,307 (GRCm39)	M1T	probably null	Het
Pwwp2a	A	G	11: 43,596,212 (GRCm39)	N184S	probably damaging	Het
Shprh	T	C	10: 11,046,157 (GRCm39)	V958A	probably damaging	Het
Smc3	A	G	19: 53,616,322 (GRCm39)	E449G	probably benign	Het
Snap91	T	A	9: 86,720,907 (GRCm39)	Y96F	possibly damaging	Het
Trim34b	A	G	7: 103,980,508 (GRCm39)	R199G	possibly damaging	Het
Unc45a	A	C	7: 79,988,680 (GRCm39)	V112G	probably damaging	Het
Vwa8	A	T	14: 79,335,782 (GRCm39)	N1413Y	probably benign	Het
Zcchc9	A	T	13: 91,954,101 (GRCm39)	N51K	probably benign	Het
Zfp628	A	G	7: 4,924,199 (GRCm39)	E807G	possibly damaging	Het

Other mutations in Zfp647

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01565:Zfp647	APN	15	76,795,870 (GRCm39)	nonsense	probably null
IGL01680:Zfp647	APN	15	76,801,968 (GRCm39)	splice site	probably benign
IGL02647:Zfp647	APN	15	76,801,915 (GRCm39)	missense	probably damaging	1.00
IGL03213:Zfp647	APN	15	76,796,177 (GRCm39)	missense	possibly damaging	0.46
IGL03401:Zfp647	APN	15	76,795,568 (GRCm39)	missense	probably damaging	1.00
R0418:Zfp647	UTSW	15	76,795,586 (GRCm39)	missense	probably damaging	1.00
R1479:Zfp647	UTSW	15	76,795,403 (GRCm39)	missense	possibly damaging	0.94
R1913:Zfp647	UTSW	15	76,796,151 (GRCm39)	missense	probably benign	0.02
R1959:Zfp647	UTSW	15	76,795,314 (GRCm39)	missense	possibly damaging	0.57
R2176:Zfp647	UTSW	15	76,795,860 (GRCm39)	missense	probably damaging	1.00
R3076:Zfp647	UTSW	15	76,802,209 (GRCm39)	start codon destroyed	probably null
R3701:Zfp647	UTSW	15	76,795,110 (GRCm39)	missense	probably damaging	1.00
R3702:Zfp647	UTSW	15	76,795,110 (GRCm39)	missense	probably damaging	1.00
R3960:Zfp647	UTSW	15	76,795,176 (GRCm39)	splice site	probably null
R4938:Zfp647	UTSW	15	76,795,244 (GRCm39)	frame shift	probably null
R4939:Zfp647	UTSW	15	76,795,244 (GRCm39)	frame shift	probably null
R5196:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5197:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5345:Zfp647	UTSW	15	76,795,695 (GRCm39)	missense	possibly damaging	0.48
R5415:Zfp647	UTSW	15	76,795,593 (GRCm39)	missense	possibly damaging	0.79
R5791:Zfp647	UTSW	15	76,802,206 (GRCm39)	missense	unknown
R5942:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5944:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5945:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5946:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R5947:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6005:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6007:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6073:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6074:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6101:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6102:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6103:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6126:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6127:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6129:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6136:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6151:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6305:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6306:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6329:Zfp647	UTSW	15	76,796,285 (GRCm39)	missense	probably damaging	0.96
R6721:Zfp647	UTSW	15	76,796,076 (GRCm39)	missense	probably benign	0.00
R7158:Zfp647	UTSW	15	76,801,505 (GRCm39)	missense	probably benign	0.01
R7239:Zfp647	UTSW	15	76,795,956 (GRCm39)	missense	probably damaging	1.00
R7611:Zfp647	UTSW	15	76,795,988 (GRCm39)	missense	probably damaging	1.00
R8066:Zfp647	UTSW	15	76,796,095 (GRCm39)	missense	probably damaging	0.98
R8170:Zfp647	UTSW	15	76,795,571 (GRCm39)	missense	possibly damaging	0.87
R8346:Zfp647	UTSW	15	76,795,928 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTCACGTGAGCTCCATAGG -3'
(R):5'- GGAACCATATGTTTGCTCAGGAC -3'

Sequencing Primer
(F):5'- CACGTGAGCTCCATAGGCAGAG -3'
(R):5'- TGCTCAGGACTCTATACTAAAGAAC -3'

Posted On

2015-02-05