Incidental Mutation 'R9407:Or5ak24'
| ID |
711574 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Or5ak24
|
| Ensembl Gene |
ENSMUSG00000075219 |
| Gene Name |
olfactory receptor family 5 subfamily AK member 24 |
| Synonyms |
MOR203-4, GA_x6K02T2Q125-46907515-46906571, Olfr994 |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.095)
|
| Stock # |
R9407 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
85260227-85266537 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 85261060 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Serine to Proline
at position 38
(S38P)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000149417
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000099925]
[ENSMUST00000214679]
[ENSMUST00000217218]
|
| AlphaFold |
Q8VF73 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000099925
AA Change: S38P
PolyPhen 2
Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000097509
Gene: ENSMUSG00000075219
AA Change: S38P
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_4
|
31 |
306 |
5.1e-48 |
PFAM |
|
Pfam:7TM_GPCR_Srsx
|
35 |
302 |
3.5e-5 |
PFAM |
|
Pfam:7tm_1
|
41 |
289 |
8.2e-20 |
PFAM |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000214679
AA Change: S38P
PolyPhen 2
Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000217218
AA Change: S38P
PolyPhen 2
Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.7%
- 20x: 99.0%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 51 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930578I06Rik |
T |
C |
14: 64,223,629 (GRCm39) |
Y49C |
probably damaging |
Het |
| Abca6 |
A |
T |
11: 110,093,210 (GRCm39) |
L1021* |
probably null |
Het |
| Abcc9 |
A |
G |
6: 142,574,229 (GRCm39) |
S1003P |
possibly damaging |
Het |
| Aldh4a1 |
A |
G |
4: 139,372,345 (GRCm39) |
I505V |
probably benign |
Het |
| Brwd1 |
T |
C |
16: 95,803,693 (GRCm39) |
D2159G |
possibly damaging |
Het |
| C1s2 |
T |
C |
6: 124,602,454 (GRCm39) |
I586V |
probably benign |
Het |
| Cd300e |
C |
A |
11: 114,946,171 (GRCm39) |
D97Y |
probably damaging |
Het |
| Celsr3 |
A |
G |
9: 108,723,596 (GRCm39) |
D2877G |
probably damaging |
Het |
| Cep41 |
T |
C |
6: 30,655,841 (GRCm39) |
N328D |
probably benign |
Het |
| Cldn15 |
G |
A |
5: 137,003,765 (GRCm39) |
V227M |
probably damaging |
Het |
| Clec10a |
T |
G |
11: 70,060,155 (GRCm39) |
I98S |
probably damaging |
Het |
| Cpne4 |
A |
G |
9: 104,749,963 (GRCm39) |
Q89R |
probably damaging |
Het |
| Cryba2 |
C |
A |
1: 74,932,037 (GRCm39) |
A4S |
probably benign |
Het |
| Csmd2 |
G |
A |
4: 128,442,613 (GRCm39) |
A3222T |
|
Het |
| Dapk1 |
C |
A |
13: 60,898,991 (GRCm39) |
Y820* |
probably null |
Het |
| Efcab5 |
G |
A |
11: 77,022,934 (GRCm39) |
T593I |
probably damaging |
Het |
| Entrep3 |
C |
A |
3: 89,094,645 (GRCm39) |
A416D |
possibly damaging |
Het |
| Gar1 |
A |
G |
3: 129,620,608 (GRCm39) |
Y160H |
probably damaging |
Het |
| Gm21834 |
T |
A |
17: 58,048,863 (GRCm39) |
I118L |
probably benign |
Het |
| Gtf2a1l |
G |
A |
17: 89,001,531 (GRCm39) |
G129D |
probably damaging |
Het |
| Gzmb |
T |
A |
14: 56,497,712 (GRCm39) |
Y176F |
probably benign |
Het |
| Helz2 |
T |
G |
2: 180,881,975 (GRCm39) |
K273Q |
probably benign |
Het |
| Ifi207 |
T |
A |
1: 173,555,234 (GRCm39) |
E816V |
probably damaging |
Het |
| Insr |
A |
C |
8: 3,235,106 (GRCm39) |
D694E |
probably benign |
Het |
| Insyn2b |
G |
A |
11: 34,352,072 (GRCm39) |
R38Q |
probably damaging |
Het |
| Ipcef1 |
A |
T |
10: 6,870,036 (GRCm39) |
C172* |
probably null |
Het |
| Itgal |
T |
G |
7: 126,921,796 (GRCm39) |
|
probably null |
Het |
| Laptm5 |
A |
T |
4: 130,655,990 (GRCm39) |
M103L |
|
Het |
| Lypd4 |
A |
T |
7: 24,566,160 (GRCm39) |
C55S |
probably null |
Het |
| Mccc1 |
A |
C |
3: 36,030,865 (GRCm39) |
H400Q |
possibly damaging |
Het |
| Myl4 |
C |
T |
11: 104,474,887 (GRCm39) |
R79W |
probably damaging |
Het |
| Myo18a |
A |
G |
11: 77,709,596 (GRCm39) |
T569A |
probably benign |
Het |
| Or14a259 |
G |
C |
7: 86,013,194 (GRCm39) |
S117* |
probably null |
Het |
| Or4c117 |
T |
A |
2: 88,955,629 (GRCm39) |
T149S |
probably benign |
Het |
| Otof |
T |
A |
5: 30,538,265 (GRCm39) |
D1153V |
probably damaging |
Het |
| Pcdha12 |
A |
T |
18: 37,153,614 (GRCm39) |
D111V |
probably damaging |
Het |
| Pomt2 |
G |
T |
12: 87,157,146 (GRCm39) |
F724L |
probably damaging |
Het |
| Ros1 |
T |
A |
10: 51,994,491 (GRCm39) |
Y1284F |
probably damaging |
Het |
| Skint6 |
T |
C |
4: 113,034,224 (GRCm39) |
K301R |
possibly damaging |
Het |
| Slc30a5 |
T |
A |
13: 100,951,214 (GRCm39) |
R223* |
probably null |
Het |
| Slc35a1 |
A |
T |
4: 34,675,181 (GRCm39) |
V119E |
probably damaging |
Het |
| Slc6a11 |
A |
T |
6: 114,220,914 (GRCm39) |
I482F |
probably damaging |
Het |
| Tektl1 |
T |
C |
10: 78,583,128 (GRCm39) |
T419A |
probably damaging |
Het |
| Tgm1 |
A |
T |
14: 55,942,991 (GRCm39) |
C616* |
probably null |
Het |
| Tiam2 |
T |
A |
17: 3,553,298 (GRCm39) |
I1125N |
probably damaging |
Het |
| Tln2 |
T |
C |
9: 67,136,732 (GRCm39) |
D1251G |
probably damaging |
Het |
| Tnfaip2 |
C |
G |
12: 111,412,161 (GRCm39) |
S187R |
probably benign |
Het |
| Ush2a |
A |
G |
1: 188,644,045 (GRCm39) |
Y4469C |
probably damaging |
Het |
| Vmn1r229 |
G |
C |
17: 21,035,259 (GRCm39) |
C168S |
probably damaging |
Het |
| Vmn2r53 |
A |
G |
7: 12,335,124 (GRCm39) |
S179P |
probably damaging |
Het |
| Zfp474 |
A |
C |
18: 52,771,502 (GRCm39) |
I52L |
probably benign |
Het |
|
| Other mutations in Or5ak24 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01140:Or5ak24
|
APN |
2 |
85,260,484 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01325:Or5ak24
|
APN |
2 |
85,260,639 (GRCm39) |
missense |
possibly damaging |
0.60 |
|
IGL01634:Or5ak24
|
APN |
2 |
85,260,783 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01809:Or5ak24
|
APN |
2 |
85,260,498 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02586:Or5ak24
|
APN |
2 |
85,260,810 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
IGL02711:Or5ak24
|
APN |
2 |
85,261,083 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R0010:Or5ak24
|
UTSW |
2 |
85,260,239 (GRCm39) |
missense |
probably benign |
|
|
R0578:Or5ak24
|
UTSW |
2 |
85,261,017 (GRCm39) |
missense |
probably benign |
0.03 |
|
R0848:Or5ak24
|
UTSW |
2 |
85,260,365 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1844:Or5ak24
|
UTSW |
2 |
85,260,265 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1912:Or5ak24
|
UTSW |
2 |
85,260,604 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R1959:Or5ak24
|
UTSW |
2 |
85,260,963 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2014:Or5ak24
|
UTSW |
2 |
85,260,696 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R2113:Or5ak24
|
UTSW |
2 |
85,260,430 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2290:Or5ak24
|
UTSW |
2 |
85,260,544 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R3622:Or5ak24
|
UTSW |
2 |
85,260,837 (GRCm39) |
missense |
probably benign |
0.12 |
|
R3918:Or5ak24
|
UTSW |
2 |
85,261,074 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R4175:Or5ak24
|
UTSW |
2 |
85,260,962 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4945:Or5ak24
|
UTSW |
2 |
85,260,895 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6132:Or5ak24
|
UTSW |
2 |
85,260,490 (GRCm39) |
missense |
probably benign |
0.06 |
|
R6439:Or5ak24
|
UTSW |
2 |
85,261,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6713:Or5ak24
|
UTSW |
2 |
85,260,883 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7065:Or5ak24
|
UTSW |
2 |
85,260,523 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7089:Or5ak24
|
UTSW |
2 |
85,260,902 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7467:Or5ak24
|
UTSW |
2 |
85,261,171 (GRCm39) |
start codon destroyed |
possibly damaging |
0.90 |
|
R8006:Or5ak24
|
UTSW |
2 |
85,260,318 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8021:Or5ak24
|
UTSW |
2 |
85,260,996 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8302:Or5ak24
|
UTSW |
2 |
85,260,430 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R8468:Or5ak24
|
UTSW |
2 |
85,260,522 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9085:Or5ak24
|
UTSW |
2 |
85,260,619 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9559:Or5ak24
|
UTSW |
2 |
85,260,753 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
| Predicted Primers |
PCR Primer
(F):5'- CAGTCACTGGTTGCAAATACAG -3'
(R):5'- AAGCCACCAAAATTGTGTAGAC -3'
Sequencing Primer
(F):5'- GATGGCAGATGTGTAGCA -3'
(R):5'- GTCATACACAAATGCAAATTCCTG -3'
|
| Posted On |
2022-05-16 |
Incidental Mutation