Mutagenetix > Incidental Mutation

Incidental Mutation 'R0125:Ros1'

21304

Institutional Source

Beutler Lab

Gene Symbol

Ros1

Ensembl Gene

ENSMUSG00000019893

Gene Name

Ros1 proto-oncogene

Synonyms

Ros-1, c-ros

MMRRC Submission

038410-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.145) question?

Stock #

R0125 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

51921817-52071340 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 52001885 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Aspartic acid at position 1079 (A1079D)

Ref Sequence

ENSEMBL: ENSMUSP00000151720 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020045] [ENSMUST00000218452] [ENSMUST00000219173] [ENSMUST00000219692]

AlphaFold

Q78DX7

Predicted Effect

probably benign
Transcript: ENSMUST00000020045
AA Change: A1100D

PolyPhen 2 Score 0.267 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000020045
Gene: ENSMUSG00000019893
AA Change: A1100D

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	568	654	2.24e-4	SMART
LY	734	776	2.28e1	SMART
LY	777	815	4.61e0	SMART
FN3	944	1023	5.53e-4	SMART
FN3	1037	1133	1.07e1	SMART
FN3	1440	1532	1.19e1	SMART
FN3	1551	1637	2.11e0	SMART
FN3	1649	1731	6.8e-4	SMART
FN3	1746	1832	2.7e1	SMART
TyrKc	1938	2208	1.3e-145	SMART
low complexity region	2294	2307	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000117992
AA Change: A1079D

PolyPhen 2 Score 0.267 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000112873
Gene: ENSMUSG00000019893
AA Change: A1079D

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
TyrKc	1917	2187	1.3e-145	SMART
low complexity region	2273	2286	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000177378
AA Change: A1079D

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000134905
Gene: ENSMUSG00000019893
AA Change: A1079D

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
Blast:LY	1190	1236	2e-18	BLAST
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
transmembrane domain	1832	1854	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000218452
AA Change: A1079D

PolyPhen 2 Score 0.385 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000219173
AA Change: A1079D

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

Predicted Effect

probably benign
Transcript: ENSMUST00000219692

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 98.9%
3x: 97.9%
10x: 94.7%
20x: 87.1%

Validation Efficiency

98% (96/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit male infertility due to impaired sperm maturation in the epididymis. Mutant sperm are capable of fertilization in vitro but not in vivo. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	T	C	14: 70,394,096 (GRCm39)		probably benign	Het
Adam23	T	C	1: 63,573,515 (GRCm39)	L261P	probably benign	Het
Adgra3	G	A	5: 50,159,194 (GRCm39)		probably benign	Het
Agtr1b	A	G	3: 20,369,704 (GRCm39)	F301L	probably benign	Het
Ahnak2	G	A	12: 112,748,776 (GRCm39)	T357I	probably benign	Het
Aldh1a7	T	C	19: 20,704,430 (GRCm39)		probably benign	Het
Apoh	A	T	11: 108,302,899 (GRCm39)	N288I	probably damaging	Het
Arfgap3	A	G	15: 83,227,340 (GRCm39)	V24A	probably benign	Het
Atp6v0a1	A	G	11: 100,929,677 (GRCm39)		probably null	Het
Axl	A	T	7: 25,486,368 (GRCm39)	M112K	probably benign	Het
Bnc2	A	C	4: 84,211,169 (GRCm39)	I425S	probably damaging	Het
Ccn4	T	C	15: 66,789,194 (GRCm39)	S227P	possibly damaging	Het
Cdc42bpa	C	T	1: 179,788,763 (GRCm39)	T30M	probably damaging	Het
Cebpz	C	A	17: 79,227,317 (GRCm39)	R1051M	possibly damaging	Het
Ces1d	A	C	8: 93,901,810 (GRCm39)		probably benign	Het
Chd1l	T	C	3: 97,494,465 (GRCm39)	N405S	probably benign	Het
Chodl	G	T	16: 78,738,311 (GRCm39)	G93V	probably damaging	Het
Cpeb2	C	T	5: 43,395,743 (GRCm39)		probably benign	Het
Crebbp	A	G	16: 3,935,105 (GRCm39)		probably benign	Het
Crybb3	T	C	5: 113,227,675 (GRCm39)	T49A	possibly damaging	Het
Ctps1	A	G	4: 120,418,722 (GRCm39)		probably benign	Het
Cyp26b1	A	G	6: 84,551,497 (GRCm39)	Y240H	probably damaging	Het
Cyp2d11	A	C	15: 82,273,422 (GRCm39)	V483G	probably benign	Het
Dennd2b	T	C	7: 109,155,545 (GRCm39)	K402E	probably benign	Het
Dnah14	A	T	1: 181,579,628 (GRCm39)	N3054Y	probably damaging	Het
Dspp	A	C	5: 104,325,905 (GRCm39)	D756A	unknown	Het
Dst	T	C	1: 34,309,984 (GRCm39)	S1553P	probably damaging	Het
Elp4	A	G	2: 105,622,559 (GRCm39)		probably null	Het
Eml6	G	T	11: 29,832,088 (GRCm39)	T194K	probably benign	Het
Evi5	A	G	5: 107,943,638 (GRCm39)	I569T	probably benign	Het
Fancm	C	T	12: 65,168,730 (GRCm39)	P1698S	possibly damaging	Het
Fhdc1	T	C	3: 84,352,852 (GRCm39)	D791G	probably benign	Het
Frem1	A	G	4: 82,930,188 (GRCm39)	Y253H	probably damaging	Het
Gpn3	A	G	5: 122,519,481 (GRCm39)	Y196C	probably benign	Het
Hcls1	A	G	16: 36,782,525 (GRCm39)	D398G	probably benign	Het
Hydin	T	C	8: 111,189,163 (GRCm39)	V1189A	probably benign	Het
Itgb3	G	A	11: 104,534,789 (GRCm39)	D549N	probably damaging	Het
Itpr2	A	G	6: 146,141,951 (GRCm39)	F1697S	probably benign	Het
Klk1b11	A	G	7: 43,648,475 (GRCm39)	T161A	probably benign	Het
Kntc1	G	A	5: 123,903,120 (GRCm39)		probably benign	Het
Map3k19	A	T	1: 127,750,837 (GRCm39)	F838Y	probably benign	Het
Map6	T	A	7: 98,985,187 (GRCm39)		probably null	Het
Mcrs1	A	G	15: 99,142,608 (GRCm39)		probably benign	Het
Mdn1	A	T	4: 32,729,956 (GRCm39)	Y2766F	probably damaging	Het
Med23	C	T	10: 24,776,686 (GRCm39)	H739Y	probably damaging	Het
Mmp17	T	A	5: 129,671,646 (GRCm39)	D65E	possibly damaging	Het
Mmp9	T	A	2: 164,793,177 (GRCm39)	L442Q	probably damaging	Het
Myo19	T	C	11: 84,779,001 (GRCm39)		probably benign	Het
Nedd1	A	C	10: 92,527,791 (GRCm39)	S468A	possibly damaging	Het
Niban2	T	A	2: 32,813,833 (GRCm39)	V682D	probably benign	Het
Nlrp4d	A	C	7: 10,116,316 (GRCm39)	V152G	probably damaging	Het
Nxf1	T	A	19: 8,740,170 (GRCm39)	D112E	probably benign	Het
Oas1h	A	T	5: 121,000,626 (GRCm39)	K79*	probably null	Het
Omg	T	A	11: 79,393,679 (GRCm39)	I60F	possibly damaging	Het
Or5p69	A	G	7: 107,967,576 (GRCm39)	Y293C	probably damaging	Het
Or8b101	A	G	9: 38,020,815 (GRCm39)	T278A	probably benign	Het
Or8b1b	T	A	9: 38,375,757 (GRCm39)	L140*	probably null	Het
Pck1	G	A	2: 172,997,874 (GRCm39)	W314*	probably null	Het
Pla2g15	T	C	8: 106,889,756 (GRCm39)	Y343H	probably benign	Het
Plcb3	T	C	19: 6,936,276 (GRCm39)	E749G	probably damaging	Het
Plgrkt	A	G	19: 29,328,442 (GRCm39)		probably null	Het
Pprc1	A	G	19: 46,057,951 (GRCm39)		probably benign	Het
Prkdc	A	T	16: 15,516,871 (GRCm39)	I1082F	probably damaging	Het
Rapgef6	T	A	11: 54,516,701 (GRCm39)	Y172*	probably null	Het
Sap30	T	C	8: 57,938,545 (GRCm39)	E147G	probably null	Het
Sell	T	C	1: 163,899,674 (GRCm39)		probably benign	Het
Senp1	A	T	15: 97,946,112 (GRCm39)	D544E	probably damaging	Het
Shpk	G	A	11: 73,105,048 (GRCm39)		probably benign	Het
Slc35b1	A	T	11: 95,277,353 (GRCm39)	T74S	probably benign	Het
Slc6a3	T	A	13: 73,718,098 (GRCm39)		probably benign	Het
Slf1	T	C	13: 77,191,864 (GRCm39)	N990S	probably benign	Het
Smgc	A	G	15: 91,738,746 (GRCm39)		probably benign	Het
Snx19	T	A	9: 30,351,515 (GRCm39)	V861D	probably damaging	Het
Sprr2e	C	T	3: 92,260,285 (GRCm39)	P39S	unknown	Het
Sstr2	T	A	11: 113,515,303 (GRCm39)	M74K	probably damaging	Het
Svep1	T	C	4: 58,099,937 (GRCm39)		probably benign	Het
Tas2r143	A	G	6: 42,377,889 (GRCm39)	I240V	probably benign	Het
Tbrg1	T	C	9: 37,563,937 (GRCm39)	I233V	probably benign	Het
Tecpr1	G	T	5: 144,134,717 (GRCm39)	D1055E	probably damaging	Het
Thap2	A	T	10: 115,212,277 (GRCm39)		probably null	Het
Tinagl1	A	G	4: 130,060,101 (GRCm39)	Y388H	probably damaging	Het
Ttn	T	A	2: 76,585,896 (GRCm39)	Y21945F	probably damaging	Het
Ugt2b1	A	T	5: 87,073,961 (GRCm39)	W133R	probably benign	Het
Usp24	C	T	4: 106,254,496 (GRCm39)	P1491L	possibly damaging	Het
Utp15	A	G	13: 98,387,390 (GRCm39)	S395P	possibly damaging	Het
Vav1	T	A	17: 57,606,847 (GRCm39)	L254Q	probably damaging	Het
Vmn2r104	T	C	17: 20,250,069 (GRCm39)	Y734C	probably damaging	Het
Vps8	T	A	16: 21,288,904 (GRCm39)	V421E	probably benign	Het
Xkr7	G	T	2: 152,874,346 (GRCm39)	A138S	probably benign	Het

Other mutations in Ros1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Ros1	APN	10	52,070,986 (GRCm39)	missense	probably benign	0.01
IGL00338:Ros1	APN	10	52,001,907 (GRCm39)	missense	probably benign
IGL00419:Ros1	APN	10	51,967,150 (GRCm39)	missense	probably damaging	0.97
IGL00840:Ros1	APN	10	52,020,969 (GRCm39)	missense	possibly damaging	0.92
IGL00841:Ros1	APN	10	52,020,969 (GRCm39)	missense	possibly damaging	0.92
IGL00951:Ros1	APN	10	52,019,348 (GRCm39)	missense	probably damaging	0.99
IGL01123:Ros1	APN	10	51,996,905 (GRCm39)	missense	probably damaging	1.00
IGL01128:Ros1	APN	10	52,018,424 (GRCm39)	nonsense	probably null
IGL01300:Ros1	APN	10	51,977,809 (GRCm39)	missense	probably benign	0.01
IGL01316:Ros1	APN	10	51,963,975 (GRCm39)	critical splice donor site	probably null
IGL01349:Ros1	APN	10	51,927,122 (GRCm39)	missense	probably damaging	0.99
IGL01363:Ros1	APN	10	52,042,238 (GRCm39)	missense	probably damaging	1.00
IGL01457:Ros1	APN	10	51,922,426 (GRCm39)	splice site	probably benign
IGL01532:Ros1	APN	10	51,967,034 (GRCm39)	splice site	probably benign
IGL01585:Ros1	APN	10	52,031,198 (GRCm39)	missense	probably damaging	1.00
IGL01650:Ros1	APN	10	52,031,075 (GRCm39)	missense	probably damaging	0.99
IGL01672:Ros1	APN	10	51,977,899 (GRCm39)	missense	possibly damaging	0.92
IGL01904:Ros1	APN	10	51,954,007 (GRCm39)	missense	probably damaging	0.97
IGL02040:Ros1	APN	10	51,992,018 (GRCm39)	missense	probably damaging	0.99
IGL02053:Ros1	APN	10	52,038,816 (GRCm39)	missense	probably damaging	1.00
IGL02147:Ros1	APN	10	51,996,991 (GRCm39)	missense	probably damaging	1.00
IGL02169:Ros1	APN	10	51,958,053 (GRCm39)	critical splice donor site	probably null
IGL02247:Ros1	APN	10	52,005,677 (GRCm39)	missense	probably damaging	0.99
IGL02262:Ros1	APN	10	52,055,065 (GRCm39)	missense	probably damaging	0.96
IGL02307:Ros1	APN	10	52,004,534 (GRCm39)	missense	possibly damaging	0.53
IGL02398:Ros1	APN	10	52,020,980 (GRCm39)	splice site	probably benign
IGL02525:Ros1	APN	10	51,992,138 (GRCm39)	missense	possibly damaging	0.66
IGL02718:Ros1	APN	10	51,994,328 (GRCm39)	missense	probably damaging	1.00
IGL02721:Ros1	APN	10	52,048,927 (GRCm39)	splice site	probably benign
IGL02808:Ros1	APN	10	52,001,985 (GRCm39)	missense	probably damaging	1.00
IGL03009:Ros1	APN	10	52,022,003 (GRCm39)	missense	probably benign	0.00
IGL03035:Ros1	APN	10	51,952,080 (GRCm39)	splice site	probably benign
IGL03092:Ros1	APN	10	51,974,902 (GRCm39)	missense	probably damaging	0.99
IGL03309:Ros1	APN	10	51,994,357 (GRCm39)	missense	possibly damaging	0.83
IGL03333:Ros1	APN	10	52,031,267 (GRCm39)	missense	probably damaging	1.00
boss	UTSW	10	51,967,091 (GRCm39)	nonsense	probably null
Chuckwagon	UTSW	10	51,994,299 (GRCm39)	missense	probably damaging	1.00
R1005_Ros1_648	UTSW	10	52,004,501 (GRCm39)	splice site	probably benign
R1220_Ros1_012	UTSW	10	51,974,966 (GRCm39)	missense	probably damaging	0.97
R3423_Ros1_122	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
trail	UTSW	10	52,037,991 (GRCm39)	nonsense	probably null
R0049:Ros1	UTSW	10	51,977,857 (GRCm39)	missense	possibly damaging	0.66
R0049:Ros1	UTSW	10	51,977,857 (GRCm39)	missense	possibly damaging	0.66
R0050:Ros1	UTSW	10	51,977,899 (GRCm39)	missense	probably damaging	0.97
R0050:Ros1	UTSW	10	51,977,899 (GRCm39)	missense	probably damaging	0.97
R0057:Ros1	UTSW	10	52,056,287 (GRCm39)	missense	probably benign	0.00
R0057:Ros1	UTSW	10	52,056,287 (GRCm39)	missense	probably benign	0.00
R0106:Ros1	UTSW	10	52,018,363 (GRCm39)	missense	possibly damaging	0.85
R0106:Ros1	UTSW	10	52,018,363 (GRCm39)	missense	possibly damaging	0.85
R0403:Ros1	UTSW	10	52,019,534 (GRCm39)	splice site	probably benign
R0487:Ros1	UTSW	10	52,031,204 (GRCm39)	missense	possibly damaging	0.69
R0502:Ros1	UTSW	10	52,070,919 (GRCm39)	splice site	probably benign
R0557:Ros1	UTSW	10	51,961,359 (GRCm39)	missense	possibly damaging	0.82
R0599:Ros1	UTSW	10	51,999,396 (GRCm39)	missense	probably damaging	1.00
R0620:Ros1	UTSW	10	51,994,444 (GRCm39)	missense	probably damaging	1.00
R0679:Ros1	UTSW	10	51,942,391 (GRCm39)	missense	possibly damaging	0.95
R1005:Ros1	UTSW	10	52,004,501 (GRCm39)	splice site	probably benign
R1073:Ros1	UTSW	10	51,922,221 (GRCm39)	missense	probably damaging	1.00
R1220:Ros1	UTSW	10	51,974,966 (GRCm39)	missense	probably damaging	0.97
R1279:Ros1	UTSW	10	52,018,262 (GRCm39)	missense	possibly damaging	0.81
R1295:Ros1	UTSW	10	51,964,028 (GRCm39)	missense	possibly damaging	0.92
R1336:Ros1	UTSW	10	52,044,758 (GRCm39)	missense	probably damaging	1.00
R1371:Ros1	UTSW	10	51,964,041 (GRCm39)	missense	probably damaging	0.98
R1447:Ros1	UTSW	10	51,974,954 (GRCm39)	missense	possibly damaging	0.66
R1486:Ros1	UTSW	10	52,048,954 (GRCm39)	missense	probably damaging	1.00
R1499:Ros1	UTSW	10	51,974,773 (GRCm39)	missense	possibly damaging	0.92
R1669:Ros1	UTSW	10	52,037,907 (GRCm39)	missense	probably damaging	1.00
R1744:Ros1	UTSW	10	51,999,475 (GRCm39)	missense	probably damaging	0.99
R1759:Ros1	UTSW	10	51,996,922 (GRCm39)	missense	probably damaging	1.00
R1791:Ros1	UTSW	10	51,976,183 (GRCm39)	missense	probably benign	0.00
R1794:Ros1	UTSW	10	52,000,199 (GRCm39)	nonsense	probably null
R2031:Ros1	UTSW	10	51,943,164 (GRCm39)	missense	possibly damaging	0.88
R2115:Ros1	UTSW	10	52,004,651 (GRCm39)	missense	probably benign	0.00
R2219:Ros1	UTSW	10	52,042,175 (GRCm39)	missense	probably damaging	1.00
R2290:Ros1	UTSW	10	51,994,477 (GRCm39)	missense	probably damaging	0.96
R2329:Ros1	UTSW	10	52,038,983 (GRCm39)	missense	probably damaging	1.00
R2371:Ros1	UTSW	10	52,039,991 (GRCm39)	missense	possibly damaging	0.66
R2879:Ros1	UTSW	10	52,048,936 (GRCm39)	critical splice donor site	probably null
R3154:Ros1	UTSW	10	51,927,077 (GRCm39)	missense	probably benign
R3423:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3424:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3425:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3433:Ros1	UTSW	10	51,967,204 (GRCm39)	missense	probably benign	0.45
R3522:Ros1	UTSW	10	51,967,091 (GRCm39)	nonsense	probably null
R3686:Ros1	UTSW	10	52,021,912 (GRCm39)	missense	probably damaging	1.00
R3710:Ros1	UTSW	10	52,037,991 (GRCm39)	nonsense	probably null
R3771:Ros1	UTSW	10	52,005,087 (GRCm39)	missense	probably damaging	0.97
R3808:Ros1	UTSW	10	51,996,944 (GRCm39)	missense	probably benign	0.08
R3930:Ros1	UTSW	10	52,070,944 (GRCm39)	missense	possibly damaging	0.92
R3950:Ros1	UTSW	10	51,942,484 (GRCm39)	missense	probably damaging	1.00
R3981:Ros1	UTSW	10	51,996,974 (GRCm39)	missense	possibly damaging	0.46
R4007:Ros1	UTSW	10	51,994,328 (GRCm39)	missense	probably damaging	1.00
R4346:Ros1	UTSW	10	52,044,705 (GRCm39)	missense	possibly damaging	0.92
R4382:Ros1	UTSW	10	51,997,055 (GRCm39)	missense	possibly damaging	0.46
R4414:Ros1	UTSW	10	52,038,800 (GRCm39)	critical splice donor site	probably null
R4450:Ros1	UTSW	10	51,954,038 (GRCm39)	missense	probably damaging	0.98
R4468:Ros1	UTSW	10	51,994,452 (GRCm39)	missense	probably damaging	1.00
R4569:Ros1	UTSW	10	52,040,090 (GRCm39)	missense	probably damaging	0.99
R4649:Ros1	UTSW	10	52,005,764 (GRCm39)	missense	possibly damaging	0.66
R4684:Ros1	UTSW	10	52,005,192 (GRCm39)	missense	probably damaging	1.00
R4706:Ros1	UTSW	10	51,977,990 (GRCm39)	missense	possibly damaging	0.95
R4731:Ros1	UTSW	10	52,018,325 (GRCm39)	missense	probably damaging	1.00
R4748:Ros1	UTSW	10	51,992,093 (GRCm39)	missense	probably benign	0.00
R4806:Ros1	UTSW	10	51,972,271 (GRCm39)	missense	probably damaging	0.96
R4865:Ros1	UTSW	10	52,048,966 (GRCm39)	missense	probably damaging	0.99
R4973:Ros1	UTSW	10	52,031,087 (GRCm39)	missense	probably damaging	0.98
R5022:Ros1	UTSW	10	52,000,171 (GRCm39)	missense	possibly damaging	0.46
R5033:Ros1	UTSW	10	52,004,512 (GRCm39)	critical splice donor site	probably null
R5082:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5083:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5130:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5269:Ros1	UTSW	10	51,927,104 (GRCm39)	missense	probably damaging	1.00
R5399:Ros1	UTSW	10	51,967,040 (GRCm39)	critical splice donor site	probably null
R5414:Ros1	UTSW	10	52,031,189 (GRCm39)	missense	probably damaging	1.00
R5659:Ros1	UTSW	10	52,019,482 (GRCm39)	missense	possibly damaging	0.92
R5742:Ros1	UTSW	10	52,018,234 (GRCm39)	critical splice donor site	probably null
R5780:Ros1	UTSW	10	52,070,953 (GRCm39)	missense	probably damaging	1.00
R5805:Ros1	UTSW	10	51,999,385 (GRCm39)	missense	probably damaging	1.00
R5843:Ros1	UTSW	10	52,042,293 (GRCm39)	missense	possibly damaging	0.92
R5881:Ros1	UTSW	10	52,057,894 (GRCm39)	missense	probably benign	0.26
R6027:Ros1	UTSW	10	52,040,064 (GRCm39)	missense	possibly damaging	0.82
R6035:Ros1	UTSW	10	51,954,067 (GRCm39)	missense	probably benign
R6035:Ros1	UTSW	10	51,954,067 (GRCm39)	missense	probably benign
R6052:Ros1	UTSW	10	52,039,999 (GRCm39)	missense	probably benign	0.39
R6175:Ros1	UTSW	10	51,977,881 (GRCm39)	missense	probably benign	0.02
R6315:Ros1	UTSW	10	51,994,306 (GRCm39)	missense	probably benign
R6342:Ros1	UTSW	10	52,031,351 (GRCm39)	missense	probably damaging	1.00
R6470:Ros1	UTSW	10	52,042,140 (GRCm39)	critical splice donor site	probably null
R6527:Ros1	UTSW	10	52,019,473 (GRCm39)	missense	possibly damaging	0.66
R6568:Ros1	UTSW	10	52,038,908 (GRCm39)	missense	probably damaging	1.00
R6573:Ros1	UTSW	10	52,031,106 (GRCm39)	missense	possibly damaging	0.84
R6653:Ros1	UTSW	10	52,018,299 (GRCm39)	missense	probably damaging	1.00
R6959:Ros1	UTSW	10	52,040,090 (GRCm39)	missense	probably damaging	0.99
R7011:Ros1	UTSW	10	52,056,272 (GRCm39)	missense	probably damaging	1.00
R7111:Ros1	UTSW	10	52,057,906 (GRCm39)	missense	probably benign	0.02
R7243:Ros1	UTSW	10	51,999,477 (GRCm39)	missense	probably damaging	1.00
R7355:Ros1	UTSW	10	52,042,175 (GRCm39)	missense	probably damaging	1.00
R7385:Ros1	UTSW	10	52,031,222 (GRCm39)	missense	probably benign	0.00
R7460:Ros1	UTSW	10	51,994,299 (GRCm39)	missense	probably damaging	1.00
R7549:Ros1	UTSW	10	52,021,930 (GRCm39)	missense	probably damaging	0.96
R7573:Ros1	UTSW	10	52,046,072 (GRCm39)	missense	probably benign	0.03
R7650:Ros1	UTSW	10	51,922,305 (GRCm39)	missense	probably benign	0.00
R7667:Ros1	UTSW	10	52,040,067 (GRCm39)	missense	probably damaging	1.00
R7696:Ros1	UTSW	10	52,018,379 (GRCm39)	missense	probably damaging	1.00
R7785:Ros1	UTSW	10	52,038,944 (GRCm39)	missense	probably damaging	1.00
R7814:Ros1	UTSW	10	51,972,233 (GRCm39)	missense	probably benign	0.28
R7830:Ros1	UTSW	10	52,031,030 (GRCm39)	missense	probably damaging	0.99
R7832:Ros1	UTSW	10	52,020,957 (GRCm39)	missense	probably damaging	0.99
R7854:Ros1	UTSW	10	52,004,563 (GRCm39)	missense	probably damaging	1.00
R7912:Ros1	UTSW	10	52,044,791 (GRCm39)	missense	probably damaging	1.00
R7972:Ros1	UTSW	10	52,030,926 (GRCm39)	nonsense	probably null
R7993:Ros1	UTSW	10	51,999,443 (GRCm39)	missense	probably benign	0.34
R8036:Ros1	UTSW	10	52,041,439 (GRCm39)	missense	probably benign
R8137:Ros1	UTSW	10	52,001,933 (GRCm39)	missense	possibly damaging	0.87
R8169:Ros1	UTSW	10	51,940,768 (GRCm39)	critical splice donor site	probably null
R8199:Ros1	UTSW	10	51,977,813 (GRCm39)	nonsense	probably null
R8293:Ros1	UTSW	10	51,964,014 (GRCm39)	missense	probably damaging	1.00
R8368:Ros1	UTSW	10	51,940,833 (GRCm39)	missense	probably damaging	1.00
R8406:Ros1	UTSW	10	51,977,941 (GRCm39)	missense	possibly damaging	0.56
R8471:Ros1	UTSW	10	51,997,078 (GRCm39)	missense	probably benign	0.00
R8498:Ros1	UTSW	10	52,055,047 (GRCm39)	missense	probably damaging	0.99
R8532:Ros1	UTSW	10	51,974,852 (GRCm39)	missense	possibly damaging	0.92
R8678:Ros1	UTSW	10	51,963,998 (GRCm39)	missense	probably benign
R8726:Ros1	UTSW	10	51,954,769 (GRCm39)	missense	possibly damaging	0.46
R8789:Ros1	UTSW	10	51,999,328 (GRCm39)	missense	probably damaging	0.99
R8799:Ros1	UTSW	10	51,922,143 (GRCm39)	missense	probably benign	0.08
R8915:Ros1	UTSW	10	51,977,805 (GRCm39)	splice site	probably benign
R8958:Ros1	UTSW	10	51,972,190 (GRCm39)	missense	probably damaging	1.00
R8972:Ros1	UTSW	10	51,999,333 (GRCm39)	missense	probably benign	0.05
R9020:Ros1	UTSW	10	52,031,023 (GRCm39)	missense	probably benign	0.32
R9147:Ros1	UTSW	10	51,927,039 (GRCm39)	missense	probably benign
R9154:Ros1	UTSW	10	51,922,301 (GRCm39)	missense	possibly damaging	0.87
R9189:Ros1	UTSW	10	52,019,502 (GRCm39)	missense	probably damaging	0.99
R9341:Ros1	UTSW	10	51,972,116 (GRCm39)	critical splice donor site	probably null
R9343:Ros1	UTSW	10	51,972,116 (GRCm39)	critical splice donor site	probably null
R9407:Ros1	UTSW	10	51,994,491 (GRCm39)	missense	probably damaging	1.00
R9428:Ros1	UTSW	10	51,958,061 (GRCm39)	missense	probably benign	0.00
R9502:Ros1	UTSW	10	52,000,174 (GRCm39)	missense	probably benign	0.00
R9531:Ros1	UTSW	10	52,007,063 (GRCm39)	missense	probably damaging	1.00
R9546:Ros1	UTSW	10	51,994,215 (GRCm39)	critical splice donor site	probably null
R9562:Ros1	UTSW	10	51,943,170 (GRCm39)	missense	probably damaging	1.00
R9565:Ros1	UTSW	10	51,943,170 (GRCm39)	missense	probably damaging	1.00
R9604:Ros1	UTSW	10	51,994,249 (GRCm39)	missense	probably damaging	1.00
R9645:Ros1	UTSW	10	51,948,148 (GRCm39)	critical splice donor site	probably null
R9658:Ros1	UTSW	10	51,967,069 (GRCm39)	missense	probably damaging	0.99
R9664:Ros1	UTSW	10	51,996,931 (GRCm39)	missense	probably benign	0.18
RF018:Ros1	UTSW	10	52,031,217 (GRCm39)	missense	probably benign
Z1176:Ros1	UTSW	10	51,967,205 (GRCm39)	missense	possibly damaging	0.89
Z1177:Ros1	UTSW	10	52,044,767 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGCCATTAAATGCTAGACTCCCAACAG -3'
(R):5'- CTACTTTTCAGTTCCATCAGCACCAGA -3'

Sequencing Primer
(F):5'- cacaactacctgtaactactgtttc -3'
(R):5'- GTTCCATCAGCACCAGAAAACC -3'

Posted On

2013-04-11