Mutagenetix > Incidental Mutation

Incidental Mutation 'R2504:Stxbp5l'

252261

Institutional Source

Beutler Lab

Gene Symbol

Stxbp5l

Ensembl Gene

ENSMUSG00000022829

Gene Name

syntaxin binding protein 5-like

Synonyms

insulin level locus 1, T2dm1, LLGL4, tomosyn-2, t2md1, A830015P08Rik

MMRRC Submission

040412-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2504 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36935304-37205324 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 36936029 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 1183 (Y1183H)

Ref Sequence

ENSEMBL: ENSMUSP00000110435 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000114780] [ENSMUST00000114781] [ENSMUST00000114782] [ENSMUST00000114787]

AlphaFold

Q5DQR4

Predicted Effect

probably damaging
Transcript: ENSMUST00000114780
AA Change: Y1102H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110428
Gene: ENSMUSG00000022829
AA Change: Y1102H

Domain	Start	End	E-Value	Type
low complexity region	16	40	N/A	INTRINSIC
WD40	58	97	1.1e2	SMART
WD40	99	138	6.66e-1	SMART
Blast:WD40	143	182	1e-20	BLAST
WD40	197	236	2.22e0	SMART
WD40	240	277	1.7e-2	SMART
Pfam:LLGL	284	396	8.6e-45	PFAM
WD40	397	476	7.7e-1	SMART
WD40	501	541	6.14e1	SMART
low complexity region	577	592	N/A	INTRINSIC
Pfam:Lgl_C	731	988	3e-9	PFAM
PDB:1URQ\|A	1038	1097	2e-25	PDB

Predicted Effect

probably damaging
Transcript: ENSMUST00000114781
AA Change: Y1126H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110429
Gene: ENSMUSG00000022829
AA Change: Y1126H

Domain	Start	End	E-Value	Type
low complexity region	16	40	N/A	INTRINSIC
WD40	58	97	1.1e2	SMART
WD40	99	138	6.66e-1	SMART
Blast:WD40	143	182	1e-20	BLAST
WD40	197	236	2.22e0	SMART
WD40	240	277	1.7e-2	SMART
Pfam:LLGL	284	396	8.9e-45	PFAM
WD40	397	476	7.7e-1	SMART
WD40	501	541	6.14e1	SMART
low complexity region	577	592	N/A	INTRINSIC
Pfam:Lgl_C	755	1012	3.1e-9	PFAM
PDB:1URQ\|A	1062	1121	2e-25	PDB

Predicted Effect

probably damaging
Transcript: ENSMUST00000114782
AA Change: Y1159H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110430
Gene: ENSMUSG00000022829
AA Change: Y1159H

Domain	Start	End	E-Value	Type
low complexity region	16	40	N/A	INTRINSIC
WD40	58	97	1.1e2	SMART
WD40	99	138	6.66e-1	SMART
Blast:WD40	143	182	1e-20	BLAST
WD40	197	236	2.22e0	SMART
WD40	240	277	1.7e-2	SMART
Pfam:LLGL	284	396	9.2e-45	PFAM
WD40	397	476	7.7e-1	SMART
WD40	501	541	6.14e1	SMART
low complexity region	577	592	N/A	INTRINSIC
Pfam:Lgl_C	785	1045	3.1e-9	PFAM
PDB:1URQ\|A	1095	1154	2e-25	PDB

Predicted Effect

probably damaging
Transcript: ENSMUST00000114787
AA Change: Y1183H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110435
Gene: ENSMUSG00000022829
AA Change: Y1183H

Domain	Start	End	E-Value	Type
low complexity region	16	40	N/A	INTRINSIC
WD40	58	97	1.1e2	SMART
WD40	99	138	6.66e-1	SMART
Blast:WD40	143	182	1e-20	BLAST
WD40	197	236	2.22e0	SMART
WD40	240	277	1.7e-2	SMART
Pfam:LLGL	287	396	8.7e-35	PFAM
WD40	397	476	7.7e-1	SMART
WD40	501	541	6.14e1	SMART
low complexity region	577	592	N/A	INTRINSIC
Pfam:Lgl_C	811	1069	3.3e-9	PFAM
PDB:1URQ\|A	1119	1178	2e-25	PDB

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is similar to syntaxin-binding protein 5 and contains ten N-terminal WD40 repeats, four variable region WD40 repeats, and a C-terminal R-SNARE domain. Studies of the orthologous proteins in mouse and rat have shown that the encoded protein may inhibit exocytosis in neurosecretory cells, and may negatively regulate the secretion of insulin. A missense variant in this gene is likely the cause of an infantile-onset neurodegenerative disorder diagnosed in two siblings of consanguineous parents. [provided by RefSeq, Jan 2017]
PHENOTYPE: Mice homozygous for a QTL derived from BTBR exhibit increased fasting serum glucose and decreased fasting serum insulin. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	T	C	11: 119,909,681 (GRCm39)	D28G	probably benign	Het
Abcg1	T	A	17: 31,311,369 (GRCm39)	S125T	probably damaging	Het
Actbl2	T	C	13: 111,392,717 (GRCm39)	S351P	possibly damaging	Het
Ankrd34b	A	G	13: 92,575,569 (GRCm39)		probably null	Het
BC051665	C	T	13: 60,930,468 (GRCm39)	V295I	probably benign	Het
C1qtnf2	A	G	11: 43,381,983 (GRCm39)	N265S	probably damaging	Het
Ccdc14	C	T	16: 34,542,220 (GRCm39)	R573*	probably null	Het
Cd55b	A	T	1: 130,337,612 (GRCm39)	Y247N	probably damaging	Het
Celsr2	A	T	3: 108,320,907 (GRCm39)	V635E	probably benign	Het
Clec16a	T	C	16: 10,377,551 (GRCm39)		probably benign	Het
Clec4b1	A	G	6: 123,042,904 (GRCm39)	Y41C	probably damaging	Het
Cntn5	A	T	9: 10,172,126 (GRCm39)	D19E	probably benign	Het
Cop1	A	G	1: 159,060,375 (GRCm39)	N53S	probably damaging	Het
Cplane1	G	A	15: 8,248,700 (GRCm39)	E1750K	probably damaging	Het
Csad	A	T	15: 102,097,102 (GRCm39)	M1K	probably null	Het
Cyb5rl	A	G	4: 106,938,142 (GRCm39)	I200V	probably benign	Het
Cyp26a1	A	G	19: 37,686,790 (GRCm39)	T81A	probably damaging	Het
Cyp2d12	A	T	15: 82,443,237 (GRCm39)	H433L	probably benign	Het
D7Ertd443e	T	A	7: 133,951,208 (GRCm39)		probably null	Het
Dennd1b	A	G	1: 139,097,908 (GRCm39)		probably benign	Het
Dmap1	G	T	4: 117,532,495 (GRCm39)	T357K	probably damaging	Het
Dzip1	G	T	14: 119,118,456 (GRCm39)	T759K	probably benign	Het
Elmo2	A	G	2: 165,140,607 (GRCm39)	V300A	probably damaging	Het
Eml5	T	C	12: 98,810,364 (GRCm39)	D864G	possibly damaging	Het
Ep400	A	G	5: 110,816,511 (GRCm39)	V2670A	probably damaging	Het
Epha3	T	A	16: 63,423,988 (GRCm39)	I534F	probably damaging	Het
Epha4	A	C	1: 77,359,628 (GRCm39)	Y742D	probably damaging	Het
Ergic2	A	G	6: 148,106,272 (GRCm39)		probably null	Het
Ero1a	A	T	14: 45,536,545 (GRCm39)		probably null	Het
Fam229a	A	G	4: 129,385,279 (GRCm39)	D70G	probably damaging	Het
Fbn2	C	T	18: 58,226,431 (GRCm39)	R781Q	probably damaging	Het
Fbxo16	A	T	14: 65,508,163 (GRCm39)		probably benign	Het
Fbxo39	T	A	11: 72,208,111 (GRCm39)	S154R	probably benign	Het
Fer	T	A	17: 64,298,575 (GRCm39)		probably null	Het
Filip1l	A	T	16: 57,391,410 (GRCm39)	D428V	probably damaging	Het
Filip1l	A	G	16: 57,391,025 (GRCm39)	I538V	possibly damaging	Het
Fsip2	T	C	2: 82,809,954 (GRCm39)	I2091T	possibly damaging	Het
Glyat	A	C	19: 12,628,762 (GRCm39)	T186P	possibly damaging	Het
Gm10604	A	G	4: 11,980,083 (GRCm39)	S74P	unknown	Het
Gm4787	T	G	12: 81,425,911 (GRCm39)	K82N	possibly damaging	Het
Hectd4	T	C	5: 121,358,683 (GRCm39)	I50T	unknown	Het
Hectd4	T	C	5: 121,402,030 (GRCm39)	S373P	possibly damaging	Het
Hmcn1	A	T	1: 150,562,618 (GRCm39)	C2313*	probably null	Het
Hrob	T	C	11: 102,146,122 (GRCm39)	Y133H	possibly damaging	Het
Igfn1	A	T	1: 135,897,054 (GRCm39)	S1171T	probably benign	Het
Ints8	T	C	4: 11,241,642 (GRCm39)	D267G	probably benign	Het
Itln1	A	G	1: 171,356,727 (GRCm39)	C251R	probably damaging	Het
Jcad	C	T	18: 4,674,026 (GRCm39)	T596M	probably damaging	Het
Kcnj16	C	T	11: 110,916,409 (GRCm39)	T357M	probably benign	Het
Kif13a	G	A	13: 46,967,676 (GRCm39)	T346M	probably damaging	Het
Klhl24	G	A	16: 19,938,917 (GRCm39)	A491T	probably benign	Het
Kntc1	C	T	5: 123,916,410 (GRCm39)	Q748*	probably null	Het
Krt25	T	A	11: 99,208,122 (GRCm39)	K369*	probably null	Het
Krt75	C	T	15: 101,476,466 (GRCm39)	R433Q	probably benign	Het
Krt76	A	G	15: 101,793,293 (GRCm39)	F582L	unknown	Het
Lysmd1	G	A	3: 95,045,708 (GRCm39)	V182I	probably benign	Het
Mab21l2	T	A	3: 86,454,862 (GRCm39)	E46V	probably damaging	Het
Magi2	A	T	5: 20,563,934 (GRCm39)	K355N	probably damaging	Het
Marchf10	T	C	11: 105,276,398 (GRCm39)	D630G	probably damaging	Het
Mast4	A	T	13: 102,875,147 (GRCm39)	I1215N	probably damaging	Het
Nckap1	G	A	2: 80,360,562 (GRCm39)	T523I	probably benign	Het
Nexmif	T	A	X: 103,127,999 (GRCm39)	D1306V	probably damaging	Het
Nfkb1	A	C	3: 135,295,090 (GRCm39)	I918R	possibly damaging	Het
Nt5el	A	G	13: 105,246,250 (GRCm39)	I270M	probably benign	Het
Nup50	A	T	15: 84,817,859 (GRCm39)	T93S	probably benign	Het
Nwd2	T	C	5: 63,961,717 (GRCm39)	Y434H	probably benign	Het
Or13a28	T	C	7: 140,218,397 (GRCm39)	V261A	probably benign	Het
Osbpl1a	C	A	18: 13,038,088 (GRCm39)	V288L	probably benign	Het
Pan3	A	G	5: 147,463,846 (GRCm39)	E562G	possibly damaging	Het
Pappa	T	A	4: 65,099,126 (GRCm39)	Y548*	probably null	Het
Phf3	A	T	1: 30,849,870 (GRCm39)	L1181Q	probably damaging	Het
Phip	T	C	9: 82,797,392 (GRCm39)	H537R	possibly damaging	Het
Pkhd1l1	T	C	15: 44,348,824 (GRCm39)	I240T	probably damaging	Het
Pole	G	A	5: 110,438,368 (GRCm39)		probably null	Het
Polq	T	A	16: 36,832,304 (GRCm39)	S15T	unknown	Het
Prrt2	T	C	7: 126,619,396 (GRCm39)	E23G	possibly damaging	Het
Prss37	A	T	6: 40,494,760 (GRCm39)		probably null	Het
Prune2	T	C	19: 16,977,400 (GRCm39)	L45P	probably damaging	Het
Psd	A	T	19: 46,313,352 (GRCm39)	M6K	possibly damaging	Het
Psmd1	A	G	1: 86,017,719 (GRCm39)	E510G	possibly damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Pxdn	T	A	12: 30,053,405 (GRCm39)	I1194N	probably damaging	Het
Rbp3	C	T	14: 33,677,975 (GRCm39)	T641M	probably damaging	Het
Rgmb	C	A	17: 16,027,909 (GRCm39)	R270L	probably benign	Het
Rpgrip1l	A	G	8: 92,007,344 (GRCm39)		probably null	Het
Rps2	G	A	17: 24,939,353 (GRCm39)		probably benign	Het
Rsbn1l	G	T	5: 21,107,364 (GRCm39)	A550E	probably damaging	Het
S1pr4	C	T	10: 81,335,138 (GRCm39)	R112H	probably benign	Het
Scfd2	T	C	5: 74,691,838 (GRCm39)	N148S	probably damaging	Het
Scin	C	T	12: 40,131,705 (GRCm39)	M276I	probably benign	Het
Sec24d	T	C	3: 123,147,255 (GRCm39)	I708T	possibly damaging	Het
Skint11	T	A	4: 114,086,009 (GRCm39)	F41I	possibly damaging	Het
Slc15a4	A	T	5: 127,694,303 (GRCm39)	F44Y	possibly damaging	Het
Slc6a18	A	G	13: 73,823,925 (GRCm39)	Y72H	probably benign	Het
Slc7a11	A	T	3: 50,332,195 (GRCm39)		probably null	Het
Slc7a14	G	T	3: 31,291,650 (GRCm39)	N209K	possibly damaging	Het
Sstr2	T	C	11: 113,515,257 (GRCm39)	C59R	probably damaging	Het
Stab1	A	G	14: 30,884,997 (GRCm39)		probably null	Het
Stag1	G	T	9: 100,748,263 (GRCm39)	S475I	probably damaging	Het
Svep1	A	T	4: 58,135,628 (GRCm39)		probably null	Het
Tm9sf2	A	G	14: 122,396,096 (GRCm39)	T653A	probably benign	Het
Tmeff1	T	C	4: 48,662,059 (GRCm39)	S366P	possibly damaging	Het
Tnnt2	G	T	1: 135,779,803 (GRCm39)	W300L	probably damaging	Het
Traj32	A	G	14: 54,423,560 (GRCm39)		probably benign	Het
Trp53bp2	A	T	1: 182,269,204 (GRCm39)	M223L	probably benign	Het
Tsga10	G	A	1: 37,854,758 (GRCm39)	T246M	probably damaging	Het
Txn2	A	T	15: 77,810,870 (GRCm39)		probably benign	Het
Ubr3	T	A	2: 69,768,542 (GRCm39)	F450I	probably damaging	Het
Usp47	T	C	7: 111,703,677 (GRCm39)		probably null	Het
Vars2	C	T	17: 35,975,685 (GRCm39)	R244Q	probably damaging	Het
Xrra1	T	A	7: 99,546,803 (GRCm39)	F251L	probably damaging	Het
Zfp804a	G	A	2: 82,087,863 (GRCm39)	R564Q	probably benign	Het
Zfp983	T	C	17: 21,877,883 (GRCm39)	C29R	probably damaging	Het

Other mutations in Stxbp5l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00836:Stxbp5l	APN	16	37,028,462 (GRCm39)	missense	possibly damaging	0.82
IGL01082:Stxbp5l	APN	16	37,024,940 (GRCm39)	missense	possibly damaging	0.89
IGL01448:Stxbp5l	APN	16	37,036,341 (GRCm39)	missense	probably damaging	0.99
IGL01475:Stxbp5l	APN	16	37,165,454 (GRCm39)	missense	possibly damaging	0.95
IGL01899:Stxbp5l	APN	16	37,020,954 (GRCm39)	missense	probably benign	0.19
IGL02232:Stxbp5l	APN	16	37,150,257 (GRCm39)	missense	probably damaging	1.00
IGL02389:Stxbp5l	APN	16	37,028,567 (GRCm39)	missense	probably benign	0.00
IGL02745:Stxbp5l	APN	16	37,007,016 (GRCm39)	nonsense	probably null
IGL03125:Stxbp5l	APN	16	37,007,083 (GRCm39)	missense	probably benign	0.02
R0058:Stxbp5l	UTSW	16	36,962,736 (GRCm39)	missense	possibly damaging	0.76
R0345:Stxbp5l	UTSW	16	37,108,670 (GRCm39)	missense	probably damaging	1.00
R0359:Stxbp5l	UTSW	16	37,036,440 (GRCm39)	splice site	probably benign
R0454:Stxbp5l	UTSW	16	36,954,646 (GRCm39)	missense	possibly damaging	0.94
R0525:Stxbp5l	UTSW	16	36,950,159 (GRCm39)	critical splice donor site	probably null
R0543:Stxbp5l	UTSW	16	37,028,458 (GRCm39)	missense	probably damaging	1.00
R0606:Stxbp5l	UTSW	16	37,024,883 (GRCm39)	missense	possibly damaging	0.46
R0607:Stxbp5l	UTSW	16	36,962,794 (GRCm39)	missense	probably benign	0.00
R1333:Stxbp5l	UTSW	16	37,068,231 (GRCm39)	critical splice donor site	probably null
R1593:Stxbp5l	UTSW	16	36,936,414 (GRCm39)	missense	probably damaging	0.96
R1605:Stxbp5l	UTSW	16	37,028,473 (GRCm39)	missense	probably benign	0.34
R1670:Stxbp5l	UTSW	16	37,111,289 (GRCm39)	critical splice donor site	probably null
R2077:Stxbp5l	UTSW	16	37,056,637 (GRCm39)	missense	possibly damaging	0.93
R2209:Stxbp5l	UTSW	16	37,036,398 (GRCm39)	missense	probably damaging	0.98
R2909:Stxbp5l	UTSW	16	37,028,548 (GRCm39)	missense	possibly damaging	0.89
R2917:Stxbp5l	UTSW	16	37,021,004 (GRCm39)	nonsense	probably null
R2918:Stxbp5l	UTSW	16	37,021,004 (GRCm39)	nonsense	probably null
R2935:Stxbp5l	UTSW	16	36,954,551 (GRCm39)	missense	possibly damaging	0.76
R3693:Stxbp5l	UTSW	16	37,061,708 (GRCm39)	nonsense	probably null
R3694:Stxbp5l	UTSW	16	37,061,708 (GRCm39)	nonsense	probably null
R3695:Stxbp5l	UTSW	16	37,061,708 (GRCm39)	nonsense	probably null
R4133:Stxbp5l	UTSW	16	37,028,481 (GRCm39)	missense	possibly damaging	0.80
R4180:Stxbp5l	UTSW	16	37,068,242 (GRCm39)	missense	probably benign	0.05
R4676:Stxbp5l	UTSW	16	37,076,246 (GRCm39)	missense	probably damaging	1.00
R4757:Stxbp5l	UTSW	16	37,008,996 (GRCm39)	missense	probably damaging	1.00
R4758:Stxbp5l	UTSW	16	36,954,592 (GRCm39)	missense	probably benign	0.18
R5105:Stxbp5l	UTSW	16	36,962,734 (GRCm39)	missense	probably benign	0.43
R5278:Stxbp5l	UTSW	16	37,007,016 (GRCm39)	missense	probably benign	0.19
R5358:Stxbp5l	UTSW	16	36,994,688 (GRCm39)	missense	probably damaging	0.99
R5411:Stxbp5l	UTSW	16	36,950,213 (GRCm39)	missense	probably damaging	1.00
R5773:Stxbp5l	UTSW	16	37,028,459 (GRCm39)	missense	probably damaging	1.00
R6539:Stxbp5l	UTSW	16	36,950,177 (GRCm39)	missense	probably damaging	1.00
R6869:Stxbp5l	UTSW	16	37,024,810 (GRCm39)	missense	possibly damaging	0.74
R6892:Stxbp5l	UTSW	16	37,008,991 (GRCm39)	missense	possibly damaging	0.94
R7369:Stxbp5l	UTSW	16	36,954,703 (GRCm39)	missense	probably benign	0.12
R7555:Stxbp5l	UTSW	16	37,143,965 (GRCm39)	missense	probably damaging	1.00
R7657:Stxbp5l	UTSW	16	37,030,534 (GRCm39)	missense	probably null	0.21
R8171:Stxbp5l	UTSW	16	37,028,416 (GRCm39)	missense	noncoding transcript
R8338:Stxbp5l	UTSW	16	36,994,718 (GRCm39)	missense	probably damaging	1.00
R8732:Stxbp5l	UTSW	16	37,061,809 (GRCm39)	missense	probably benign
R8833:Stxbp5l	UTSW	16	37,024,814 (GRCm39)	missense	probably benign	0.44
R8883:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R8898:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R8899:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R8906:Stxbp5l	UTSW	16	37,028,526 (GRCm39)	missense	probably damaging	1.00
R8918:Stxbp5l	UTSW	16	36,954,892 (GRCm39)	missense
R8959:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R8961:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R8989:Stxbp5l	UTSW	16	37,036,414 (GRCm39)	frame shift	probably null
R9027:Stxbp5l	UTSW	16	37,165,473 (GRCm39)	missense	probably damaging	1.00
R9044:Stxbp5l	UTSW	16	37,024,930 (GRCm39)	missense	possibly damaging	0.77
R9226:Stxbp5l	UTSW	16	37,076,206 (GRCm39)	missense	probably damaging	0.96
R9284:Stxbp5l	UTSW	16	37,028,442 (GRCm39)	nonsense	probably null
R9351:Stxbp5l	UTSW	16	36,936,047 (GRCm39)	missense	probably damaging	1.00
R9425:Stxbp5l	UTSW	16	36,994,706 (GRCm39)	missense	possibly damaging	0.83
R9545:Stxbp5l	UTSW	16	37,028,625 (GRCm39)	critical splice acceptor site	probably null
R9567:Stxbp5l	UTSW	16	37,061,734 (GRCm39)	missense	probably benign	0.37
R9616:Stxbp5l	UTSW	16	37,036,314 (GRCm39)	missense	probably damaging	1.00
R9781:Stxbp5l	UTSW	16	37,165,485 (GRCm39)	missense	probably benign	0.38
Z1088:Stxbp5l	UTSW	16	37,024,851 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TAACAGGGGTTCCAGCCTTTC -3'
(R):5'- GCAGAAGCGTTTTCCAAGC -3'

Sequencing Primer
(F):5'- AGCCTTTCTGTTGTACAGCATC -3'
(R):5'- GCTCAGTAGTGATTTGCTAAG -3'

Posted On

2014-12-04