Mutagenetix > Incidental Mutation

Incidental Mutation 'R4152:Vmn2r100'

314970

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r100

Ensembl Gene

ENSMUSG00000091859

Gene Name

vomeronasal 2, receptor 100

Synonyms

EG627537

MMRRC Submission

040996-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R4152 (G1)

Quality Score

217

Status

Validated

Chromosome

Chromosomal Location

19725073-19752322 bp(+) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

AAAACAGGAGTATTGATTGGAAAC to AAAAC at 19743681 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000128350 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166081] [ENSMUST00000231465]

AlphaFold

E9QAZ9

Predicted Effect

probably null
Transcript: ENSMUST00000166081

SMART Domains

Protein: ENSMUSP00000128350
Gene: ENSMUSG00000091859

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	83	456	7.4e-41	PFAM
Pfam:NCD3G	510	563	1.9e-21	PFAM
Pfam:7tm_3	594	831	2.6e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231465

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.7%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acaca	T	G	11: 84,183,752 (GRCm39)	M31R	possibly damaging	Het
Akap6	A	C	12: 53,187,190 (GRCm39)	S1535R	probably benign	Het
Ap3b2	A	G	7: 81,127,765 (GRCm39)	I137T	probably damaging	Het
Cdc16	A	G	8: 13,812,857 (GRCm39)	S36G	probably damaging	Het
Clcn4	T	C	7: 7,297,833 (GRCm39)	N67D	probably benign	Het
Col4a1	T	C	8: 11,267,227 (GRCm39)		probably null	Het
Crem	G	T	18: 3,288,055 (GRCm39)	N179K	probably damaging	Het
Fam78b	T	C	1: 166,906,369 (GRCm39)	M176T	probably benign	Het
Fcgbpl1	T	A	7: 27,856,322 (GRCm39)	H2036Q	possibly damaging	Het
Gcn1	A	G	5: 115,751,413 (GRCm39)		probably null	Het
Gm6483	T	C	8: 19,737,926 (GRCm39)		noncoding transcript	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Klk1b16	T	C	7: 43,789,973 (GRCm39)	F81S	probably benign	Het
Lpgat1	C	A	1: 191,451,600 (GRCm39)	Y36*	probably null	Het
Mavs	A	G	2: 131,088,528 (GRCm39)	D444G	probably benign	Het
Ndst3	T	C	3: 123,465,876 (GRCm39)	Y32C	probably damaging	Het
Nemp2	A	G	1: 52,680,210 (GRCm39)	S145G	probably benign	Het
Or52b1	T	C	7: 104,978,592 (GRCm39)	N269S	probably damaging	Het
Or7g20	T	A	9: 18,946,816 (GRCm39)	Y132*	probably null	Het
Or8g52	A	C	9: 39,631,296 (GRCm39)	M258L	probably benign	Het
Pds5a	A	T	5: 65,823,514 (GRCm39)	C92*	probably null	Het
Pgk2	A	G	17: 40,519,149 (GRCm39)	V93A	probably damaging	Het
Phf3	C	T	1: 30,870,539 (GRCm39)	V116I	probably benign	Het
Prl8a2	T	C	13: 27,534,985 (GRCm39)	Y86H	possibly damaging	Het
Rab4b	T	C	7: 26,875,551 (GRCm39)		probably benign	Het
Rsad1	T	C	11: 94,439,449 (GRCm39)		probably benign	Het
Sim1	G	A	10: 50,859,950 (GRCm39)	C604Y	probably damaging	Het
Slc5a3	T	A	16: 91,874,696 (GRCm39)	L251*	probably null	Het
Slit3	A	G	11: 35,589,147 (GRCm39)	N1234S	probably damaging	Het
Sntg1	A	T	1: 8,653,569 (GRCm39)		probably null	Het
Snx31	T	C	15: 36,525,785 (GRCm39)	N305D	probably benign	Het
St14	T	C	9: 31,001,802 (GRCm39)	I768V	probably benign	Het
Tep1	G	A	14: 51,075,051 (GRCm39)	H1755Y	possibly damaging	Het
Tlr6	A	C	5: 65,110,555 (GRCm39)	F784C	probably damaging	Het
Tmem132a	A	G	19: 10,836,427 (GRCm39)	V701A	probably benign	Het
Tspan15	A	T	10: 62,025,621 (GRCm39)	M197K	possibly damaging	Het
Upf1	C	T	8: 70,791,110 (GRCm39)	R544H	probably damaging	Het
Vegfb	T	C	19: 6,963,446 (GRCm39)	Y106C	probably damaging	Het
Vmn2r18	T	A	5: 151,485,730 (GRCm39)	Q588L	probably damaging	Het
Vmn2r66	T	C	7: 84,654,800 (GRCm39)	D503G	probably benign	Het
Zc3h15	T	C	2: 83,488,913 (GRCm39)	V161A	probably benign	Het

Other mutations in Vmn2r100

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00904:Vmn2r100	APN	17	19,746,262 (GRCm39)	missense	probably damaging	1.00
IGL00912:Vmn2r100	APN	17	19,751,654 (GRCm39)	missense	possibly damaging	0.95
IGL01107:Vmn2r100	APN	17	19,741,618 (GRCm39)	missense	probably damaging	1.00
IGL01517:Vmn2r100	APN	17	19,742,225 (GRCm39)	missense	probably benign	0.37
IGL01594:Vmn2r100	APN	17	19,751,495 (GRCm39)	missense	possibly damaging	0.52
IGL01657:Vmn2r100	APN	17	19,746,178 (GRCm39)	missense	possibly damaging	0.89
IGL01822:Vmn2r100	APN	17	19,725,100 (GRCm39)	missense	probably null	0.00
IGL02020:Vmn2r100	APN	17	19,725,200 (GRCm39)	missense	possibly damaging	0.78
IGL02060:Vmn2r100	APN	17	19,741,516 (GRCm39)	missense	possibly damaging	0.79
IGL02126:Vmn2r100	APN	17	19,741,504 (GRCm39)	splice site	probably benign
IGL02142:Vmn2r100	APN	17	19,742,583 (GRCm39)	missense	probably damaging	1.00
IGL02308:Vmn2r100	APN	17	19,741,597 (GRCm39)	missense	possibly damaging	0.90
IGL02407:Vmn2r100	APN	17	19,741,770 (GRCm39)	missense	probably damaging	0.98
IGL02469:Vmn2r100	APN	17	19,751,547 (GRCm39)	nonsense	probably null
IGL03088:Vmn2r100	APN	17	19,742,301 (GRCm39)	missense	probably benign	0.27
IGL03181:Vmn2r100	APN	17	19,752,207 (GRCm39)	missense	probably damaging	1.00
IGL03405:Vmn2r100	APN	17	19,752,186 (GRCm39)	missense	probably damaging	1.00
H8562:Vmn2r100	UTSW	17	19,741,752 (GRCm39)	missense	possibly damaging	0.87
R0012:Vmn2r100	UTSW	17	19,746,296 (GRCm39)	missense	probably damaging	0.99
R0012:Vmn2r100	UTSW	17	19,725,136 (GRCm39)	missense	probably benign
R0044:Vmn2r100	UTSW	17	19,742,441 (GRCm39)	missense	possibly damaging	0.46
R0109:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0111:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0112:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0149:Vmn2r100	UTSW	17	19,741,509 (GRCm39)	critical splice acceptor site	probably null
R0355:Vmn2r100	UTSW	17	19,751,582 (GRCm39)	missense	probably benign	0.00
R0395:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0396:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0453:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0465:Vmn2r100	UTSW	17	19,751,792 (GRCm39)	missense	probably damaging	0.98
R0477:Vmn2r100	UTSW	17	19,742,776 (GRCm39)	missense	probably benign	0.00
R0510:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0512:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0514:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0518:Vmn2r100	UTSW	17	19,742,178 (GRCm39)	missense	probably damaging	1.00
R0521:Vmn2r100	UTSW	17	19,742,178 (GRCm39)	missense	probably damaging	1.00
R0555:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0608:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0959:Vmn2r100	UTSW	17	19,743,786 (GRCm39)	missense	possibly damaging	0.95
R1114:Vmn2r100	UTSW	17	19,752,261 (GRCm39)	missense	probably damaging	1.00
R2027:Vmn2r100	UTSW	17	19,742,334 (GRCm39)	missense	probably benign	0.02
R2049:Vmn2r100	UTSW	17	19,742,312 (GRCm39)	missense	probably benign	0.00
R2224:Vmn2r100	UTSW	17	19,742,634 (GRCm39)	missense	probably benign	0.03
R2226:Vmn2r100	UTSW	17	19,742,634 (GRCm39)	missense	probably benign	0.03
R3618:Vmn2r100	UTSW	17	19,743,692 (GRCm39)	missense	probably benign
R3715:Vmn2r100	UTSW	17	19,752,272 (GRCm39)	missense	probably damaging	0.99
R4120:Vmn2r100	UTSW	17	19,752,215 (GRCm39)	missense	probably damaging	1.00
R4153:Vmn2r100	UTSW	17	19,743,681 (GRCm39)	frame shift	probably null
R4154:Vmn2r100	UTSW	17	19,743,681 (GRCm39)	frame shift	probably null
R4200:Vmn2r100	UTSW	17	19,742,797 (GRCm39)	missense	probably benign	0.29
R4632:Vmn2r100	UTSW	17	19,752,216 (GRCm39)	missense	probably damaging	1.00
R4720:Vmn2r100	UTSW	17	19,742,788 (GRCm39)	missense	probably benign	0.02
R4761:Vmn2r100	UTSW	17	19,741,630 (GRCm39)	missense	possibly damaging	0.47
R4831:Vmn2r100	UTSW	17	19,741,672 (GRCm39)	missense	probably benign	0.28
R4951:Vmn2r100	UTSW	17	19,752,300 (GRCm39)	missense	probably benign	0.01
R5211:Vmn2r100	UTSW	17	19,746,257 (GRCm39)	missense	possibly damaging	0.67
R5553:Vmn2r100	UTSW	17	19,725,110 (GRCm39)	missense	possibly damaging	0.64
R5657:Vmn2r100	UTSW	17	19,725,178 (GRCm39)	missense	probably benign	0.31
R5883:Vmn2r100	UTSW	17	19,743,786 (GRCm39)	missense	probably benign
R5912:Vmn2r100	UTSW	17	19,752,071 (GRCm39)	missense	probably damaging	0.99
R6141:Vmn2r100	UTSW	17	19,742,576 (GRCm39)	missense	probably benign	0.07
R6146:Vmn2r100	UTSW	17	19,742,522 (GRCm39)	missense	probably benign	0.04
R6500:Vmn2r100	UTSW	17	19,742,355 (GRCm39)	missense	probably damaging	1.00
R6575:Vmn2r100	UTSW	17	19,741,671 (GRCm39)	missense	probably benign	0.12
R6647:Vmn2r100	UTSW	17	19,742,785 (GRCm39)	missense	probably benign	0.00
R7038:Vmn2r100	UTSW	17	19,725,263 (GRCm39)	missense	possibly damaging	0.76
R7052:Vmn2r100	UTSW	17	19,751,556 (GRCm39)	missense	possibly damaging	0.95
R7170:Vmn2r100	UTSW	17	19,752,233 (GRCm39)	missense	probably benign	0.00
R7209:Vmn2r100	UTSW	17	19,751,576 (GRCm39)	missense	not run
R7312:Vmn2r100	UTSW	17	19,742,296 (GRCm39)	missense	probably benign	0.01
R7734:Vmn2r100	UTSW	17	19,742,296 (GRCm39)	missense	probably benign	0.01
R7750:Vmn2r100	UTSW	17	19,742,726 (GRCm39)	missense	probably benign
R8103:Vmn2r100	UTSW	17	19,751,415 (GRCm39)	splice site	probably null
R8193:Vmn2r100	UTSW	17	19,725,102 (GRCm39)	nonsense	probably null
R8267:Vmn2r100	UTSW	17	19,742,752 (GRCm39)	nonsense	probably null
R8290:Vmn2r100	UTSW	17	19,751,612 (GRCm39)	missense	probably damaging	0.99
R8531:Vmn2r100	UTSW	17	19,742,459 (GRCm39)	missense	possibly damaging	0.66
R8786:Vmn2r100	UTSW	17	19,742,838 (GRCm39)	missense	probably damaging	1.00
R8920:Vmn2r100	UTSW	17	19,741,620 (GRCm39)	missense	probably damaging	1.00
R8938:Vmn2r100	UTSW	17	19,751,825 (GRCm39)	missense	probably benign	0.00
R9555:Vmn2r100	UTSW	17	19,743,857 (GRCm39)	missense	probably benign	0.00
R9572:Vmn2r100	UTSW	17	19,741,513 (GRCm39)	missense	probably benign	0.00
R9609:Vmn2r100	UTSW	17	19,743,732 (GRCm39)	missense	probably damaging	1.00
R9618:Vmn2r100	UTSW	17	19,742,583 (GRCm39)	missense	probably damaging	1.00
X0062:Vmn2r100	UTSW	17	19,751,652 (GRCm39)	missense	possibly damaging	0.89
Z1176:Vmn2r100	UTSW	17	19,741,792 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn2r100	UTSW	17	19,725,251 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGTGGGTATTGCTAGGTTTCAAAAG -3'
(R):5'- CCCACTTCTGAAAATATTTCTGGC -3'

Sequencing Primer
(F):5'- CTTGGTTCAAAGTTTTCATGTGAAG -3'
(R):5'- CTTCTGAAAATATTTCTGGCCATTGG -3'

Posted On

2015-05-14