|Institutional Source||Beutler Lab|
|Gene Name||CART prepropeptide|
|Is this an essential gene?||Possibly non essential (E-score: 0.405)|
|Stock #||R4670 (G1)|
|Chromosomal Location||99898489-99901091 bp(-) (GRCm38)|
|Type of Mutation||critical splice acceptor site|
|DNA Base Change (assembly)||C to T at 99900080 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000022150 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000022150] [ENSMUST00000224142]|
|Predicted Effect||probably null
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.6332|
|Coding Region Coverage||
|Validation Efficiency||97% (69/71)|
FUNCTION: This gene encodes preproprotein isoforms that are processed into multiple biologically active peptides. Expression of this gene is regulated by cocaine and other drugs, and is associated with feeding/appetite and stress response. Mice lacking the encoded protein are predisposed to obesity. Deficiency of the encoded protein in mice results in pancreatic islet dysfunction, impaired insulin secretion and glucose intolerance. Alternative splicing results in multiple transcript variants encoding different isoforms, which are subsequently processed into mature peptides. [provided by RefSeq, Jul 2015]
PHENOTYPE: Homozygous null mice exhibit increases in food consumption, body weight, and fat mass when fed a high-fat diet, but not on a regular diet. Mice homozygous for another disruption in this gene have increased body weight at older ages, impaired insulin secretion and glucose intolerance. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cartpt||
(F):5'- AAGACCGTGTGTAAGATTCTAGGC -3'
(R):5'- CGAGCGAATCCCTAGTTGTG -3'
(F):5'- AGATTCTAGGCAATCTTTTCTCGTG -3'
(R):5'- AATCCCTAGTTGTGGCCGGTC -3'