Mutagenetix > Incidental Mutation

Incidental Mutation 'R4915:St14'

379953

Institutional Source

Beutler Lab

Gene Symbol

St14

Ensembl Gene

ENSMUSG00000031995

Gene Name

suppression of tumorigenicity 14 (colon carcinoma)

Synonyms

Tmprss14, matriptase, Prss14, Epithin, MT-SP1

MMRRC Submission

042517-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4915 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

31000698-31043149 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 31019960 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 50 (R50*)

Ref Sequence

ENSEMBL: ENSMUSP00000034478 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034478] [ENSMUST00000123557] [ENSMUST00000214418]

AlphaFold

P56677

Predicted Effect

probably null
Transcript: ENSMUST00000034478
AA Change: R50*

SMART Domains

Protein: ENSMUSP00000034478
Gene: ENSMUSG00000031995
AA Change: R50*

Domain	Start	End	E-Value	Type
transmembrane domain	55	77	N/A	INTRINSIC
Pfam:SEA	88	181	7.9e-17	PFAM
CUB	214	334	4.24e-14	SMART
CUB	340	447	4.37e-25	SMART
LDLa	452	486	2.31e-9	SMART
LDLa	487	523	4.08e-10	SMART
LDLa	524	561	3.98e-13	SMART
LDLa	566	604	1.48e-7	SMART
Tryp_SPc	614	849	1.25e-93	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000123557

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148789

Predicted Effect

probably benign
Transcript: ENSMUST00000214418

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 98.9%
3x: 98.0%
10x: 95.1%
20x: 87.9%

Validation Efficiency

98% (162/166)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous inactivation of this locus results in pleiotropic defects affecting the development of the epidermis, hair follicles, and immune system. Mutant mice become dehydrated due to impaired epidermal barrier function and die within days of birth. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 135 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	C	T	12: 71,236,420 (GRCm39)	A1257V	possibly damaging	Het
Aldh8a1	T	C	10: 21,271,662 (GRCm39)	S463P	probably damaging	Het
Angptl1	T	G	1: 156,672,388 (GRCm39)	D71E	probably benign	Het
Ank2	T	A	3: 126,736,320 (GRCm39)		probably benign	Het
Ano1	A	G	7: 144,165,112 (GRCm39)	S649P	possibly damaging	Het
Arhgef40	A	G	14: 52,227,556 (GRCm39)	E434G	probably damaging	Het
Asb15	A	T	6: 24,566,292 (GRCm39)	D415V	probably damaging	Het
Baz2b	T	A	2: 59,744,387 (GRCm39)	T1373S	possibly damaging	Het
Bcas1	T	C	2: 170,220,806 (GRCm39)	D324G	probably damaging	Het
Bfsp1	C	T	2: 143,669,391 (GRCm39)	R396Q	probably benign	Het
Bmi1	G	A	2: 18,687,143 (GRCm39)		probably benign	Het
Bpifb9b	T	A	2: 154,156,026 (GRCm39)		probably null	Het
Brd9	A	G	13: 74,086,574 (GRCm39)	E25G	probably damaging	Het
Btbd7	A	T	12: 102,804,046 (GRCm39)	C331*	probably null	Het
Ccdc198	A	T	14: 49,470,351 (GRCm39)	N189K	probably benign	Het
Cdh26	T	C	2: 178,091,614 (GRCm39)	S58P	probably benign	Het
Cenpj	T	C	14: 56,791,175 (GRCm39)	D328G	probably damaging	Het
Cherp	T	C	8: 73,222,241 (GRCm39)	D255G	probably damaging	Het
Clec5a	T	A	6: 40,562,165 (GRCm39)		probably benign	Het
Cntnap2	T	C	6: 46,506,969 (GRCm39)		probably benign	Het
Col7a1	G	A	9: 108,795,532 (GRCm39)	G1529E	unknown	Het
Cybb	C	G	X: 9,316,989 (GRCm39)	D246H	probably benign	Het
Cyp2a22	C	A	7: 26,637,195 (GRCm39)	E196D	probably benign	Het
Cyp4f18	T	A	8: 72,762,898 (GRCm39)	H63L	probably damaging	Het
Dclk2	A	T	3: 86,732,049 (GRCm39)		probably null	Het
Ddx6	T	A	9: 44,524,170 (GRCm39)	D82E	probably damaging	Het
Defb42	A	G	14: 63,285,790 (GRCm39)	I57V	probably benign	Het
Dennd5a	A	G	7: 109,500,296 (GRCm39)	F943S	probably damaging	Het
Dip2c	T	A	13: 9,671,905 (GRCm39)		probably null	Het
Disp2	A	T	2: 118,620,935 (GRCm39)	S556C	probably damaging	Het
Dnah12	A	T	14: 26,455,725 (GRCm39)	D816V	probably damaging	Het
Dpy19l3	T	C	7: 35,452,167 (GRCm39)		probably benign	Het
Efcab11	T	C	12: 99,685,321 (GRCm39)	D151G	probably damaging	Het
Ehbp1	T	C	11: 22,096,592 (GRCm39)	D299G	probably benign	Het
Evl	G	T	12: 108,652,365 (GRCm39)	R359L	probably damaging	Het
Fasn	T	G	11: 120,707,472 (GRCm39)	N799T	probably benign	Het
Fbxo3	T	G	2: 103,885,311 (GRCm39)	N388K	probably damaging	Het
Fbxw14	A	T	9: 109,103,592 (GRCm39)	F40Y	possibly damaging	Het
Fbxw22	A	G	9: 109,213,009 (GRCm39)	F313L	probably damaging	Het
Fer1l4	T	C	2: 155,873,220 (GRCm39)	K1287E	probably benign	Het
Fn1	A	T	1: 71,634,968 (GRCm39)		probably null	Het
Fry	A	T	5: 150,402,328 (GRCm39)	T790S	probably benign	Het
Fut8	G	T	12: 77,521,818 (GRCm39)	A486S	probably damaging	Het
Gfra1	A	T	19: 58,255,522 (GRCm39)	S308R	probably damaging	Het
Gm13030	T	A	4: 138,601,239 (GRCm39)		probably benign	Het
Gm37150	C	T	9: 72,292,772 (GRCm39)		noncoding transcript	Het
Gm6788	C	T	19: 28,740,664 (GRCm39)		noncoding transcript	Het
Gm8122	T	C	14: 43,091,573 (GRCm39)	N65S	unknown	Het
Gnb4	C	T	3: 32,639,236 (GRCm39)		probably benign	Het
Gprin1	G	T	13: 54,885,886 (GRCm39)	P796Q	probably damaging	Het
Grin1	A	G	2: 25,188,565 (GRCm39)		probably benign	Het
Helz2	T	C	2: 180,874,231 (GRCm39)	R2088G	possibly damaging	Het
Hsd3b9	C	T	3: 98,357,845 (GRCm39)	V56M	probably damaging	Het
Ighv6-6	C	A	12: 114,398,595 (GRCm39)	R57L	probably damaging	Het
Inhca	A	C	9: 103,129,054 (GRCm39)	Y235*	probably null	Het
Inpp5f	A	T	7: 128,286,840 (GRCm39)	D573V	probably damaging	Het
Iqgap3	T	A	3: 88,008,834 (GRCm39)	I643K	possibly damaging	Het
Itga11	C	A	9: 62,659,530 (GRCm39)	Y427*	probably null	Het
Kbtbd8	T	G	6: 95,103,515 (GRCm39)	M388R	possibly damaging	Het
Kif1a	T	A	1: 93,002,700 (GRCm39)	E233V	probably benign	Het
Krt10	T	C	11: 99,278,334 (GRCm39)	N275S	probably damaging	Het
Lrrc3	T	C	10: 77,737,253 (GRCm39)	D61G	probably benign	Het
Mapk8ip3	A	G	17: 25,128,127 (GRCm39)	S377P	possibly damaging	Het
Mccc1	A	G	3: 36,051,703 (GRCm39)	L32S	probably benign	Het
Meis1	T	C	11: 18,959,222 (GRCm39)		probably benign	Het
Mmp11	C	T	10: 75,761,419 (GRCm39)	A31T	probably damaging	Het
Mthfsl	A	C	9: 88,597,550 (GRCm39)	L67V	probably damaging	Het
Myo1c	T	G	11: 75,547,135 (GRCm39)	M1R	probably null	Het
N4bp2	T	A	5: 65,960,847 (GRCm39)	M506K	probably damaging	Het
Nfix	T	C	8: 85,498,458 (GRCm39)	I172V	probably benign	Het
Nid1	A	G	13: 13,674,171 (GRCm39)	E850G	possibly damaging	Het
Nlrp9c	T	C	7: 26,083,885 (GRCm39)	T565A	probably benign	Het
Nomo1	T	C	7: 45,693,656 (GRCm39)	F163L	probably benign	Het
Nsd1	T	C	13: 55,395,681 (GRCm39)	V1197A	possibly damaging	Het
Nsd1	A	G	13: 55,424,341 (GRCm39)	T1463A	probably benign	Het
Nsf	G	A	11: 103,801,185 (GRCm39)		probably benign	Het
Or3a1b	A	T	11: 74,012,705 (GRCm39)	I197F	probably benign	Het
Or4k51	A	T	2: 111,584,725 (GRCm39)	I44F	probably benign	Het
Or5an1c	A	G	19: 12,218,737 (GRCm39)	V96A	probably benign	Het
Or5b106	T	A	19: 13,123,355 (GRCm39)	I223L	possibly damaging	Het
Or8k33	A	G	2: 86,384,399 (GRCm39)	L23P	probably damaging	Het
Or9g10	T	C	2: 85,584,465 (GRCm39)		probably benign	Het
Pcdha6	T	A	18: 37,101,510 (GRCm39)	D234E	probably damaging	Het
Pcnx1	C	A	12: 82,021,269 (GRCm39)	F1425L	probably benign	Het
Pdzd9	T	G	7: 120,269,391 (GRCm39)	N10T	possibly damaging	Het
Pex6	A	T	17: 47,024,982 (GRCm39)	H345L	probably damaging	Het
Pfkfb3	G	A	2: 11,495,109 (GRCm39)	Q100*	probably null	Het
Pgm2	G	A	5: 64,258,291 (GRCm39)	G92E	probably damaging	Het
Pip4k2c	T	C	10: 127,035,196 (GRCm39)	T391A	possibly damaging	Het
Plcxd2	C	T	16: 45,800,941 (GRCm39)	W94*	probably null	Het
Ppm1k	T	A	6: 57,487,762 (GRCm39)	N354Y	probably damaging	Het
Prkcg	T	G	7: 3,378,781 (GRCm39)	Y624*	probably null	Het
Rabep2	T	C	7: 126,044,094 (GRCm39)	S517P	probably damaging	Het
Rabgap1l	A	G	1: 160,269,412 (GRCm39)	I717T	probably benign	Het
Rasef	A	G	4: 73,649,696 (GRCm39)	C484R	probably damaging	Het
Rdh19	A	G	10: 127,686,113 (GRCm39)	D75G	probably benign	Het
Rpl21-ps10	T	C	3: 38,161,617 (GRCm39)		noncoding transcript	Het
Rsl1d1	A	T	16: 11,017,593 (GRCm39)		probably null	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Rwdd1	T	C	10: 33,885,074 (GRCm39)	D62G	possibly damaging	Het
Scin	T	A	12: 40,119,373 (GRCm39)	I552F	possibly damaging	Het
Scn3a	T	C	2: 65,291,799 (GRCm39)	N1649S	probably damaging	Het
Slc20a2	C	T	8: 23,051,020 (GRCm39)	S351L	probably damaging	Het
Slc24a1	A	T	9: 64,855,213 (GRCm39)	F565I	unknown	Het
Slc4a1	A	G	11: 102,243,279 (GRCm39)	V784A	probably damaging	Het
Slf2	T	G	19: 44,960,100 (GRCm39)	D1022E	probably damaging	Het
Sox6	T	C	7: 115,076,199 (GRCm39)	D814G	probably damaging	Het
Spata4	T	C	8: 55,055,471 (GRCm39)		probably null	Het
Spats2l	A	T	1: 57,941,347 (GRCm39)	K202M	probably damaging	Het
Speer4b	C	T	5: 27,705,134 (GRCm39)	E80K	probably benign	Het
Sqle	T	G	15: 59,193,218 (GRCm39)	Y198*	probably null	Het
Srek1	T	C	13: 103,889,194 (GRCm39)		probably benign	Het
Srek1	T	C	13: 103,889,071 (GRCm39)		probably benign	Het
Tcaf1	A	G	6: 42,652,130 (GRCm39)	V784A	probably damaging	Het
Tfr2	C	T	5: 137,581,673 (GRCm39)	R587W	probably damaging	Het
Tmpo	A	G	10: 90,985,411 (GRCm39)	V357A	probably damaging	Het
Trrap	A	T	5: 144,742,545 (GRCm39)	I1101F	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Ttpal	G	A	2: 163,449,397 (GRCm39)	R84H	probably damaging	Het
Tyk2	T	C	9: 21,022,433 (GRCm39)	T799A	probably benign	Het
Ugt1a10	A	T	1: 87,983,646 (GRCm39)	D148V	probably damaging	Het
Usp29	C	T	7: 6,964,504 (GRCm39)	P116S	probably benign	Het
Usp8	A	T	2: 126,562,060 (GRCm39)	K85*	probably null	Het
Usp9y	C	T	Y: 1,316,735 (GRCm39)	R1938H	probably damaging	Het
Vmn1r90	T	G	7: 14,295,950 (GRCm39)	R49S	possibly damaging	Het
Vmn2r71	C	A	7: 85,270,476 (GRCm39)	N547K	probably damaging	Het
Vps45	T	C	3: 95,926,943 (GRCm39)	T535A	probably damaging	Het
Yars1	A	T	4: 129,104,384 (GRCm39)		probably benign	Het
Zfp114	T	C	7: 23,877,290 (GRCm39)	L44P	probably damaging	Het
Zfp36l2	A	G	17: 84,493,690 (GRCm39)		probably benign	Het
Zfp512	T	C	5: 31,634,209 (GRCm39)	S407P	probably damaging	Het
Zfp574	C	T	7: 24,780,151 (GRCm39)	P391L	probably damaging	Het
Zfp607a	T	G	7: 27,577,985 (GRCm39)	C352G	probably benign	Het
Zfp791	T	A	8: 85,837,580 (GRCm39)	I95L	probably benign	Het
Zfr	A	C	15: 12,162,198 (GRCm39)		probably null	Het

Other mutations in St14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00781:St14	APN	9	31,015,075 (GRCm39)	missense	probably damaging	1.00
IGL01443:St14	APN	9	31,011,489 (GRCm39)	nonsense	probably null
IGL01816:St14	APN	9	31,019,563 (GRCm39)	missense	possibly damaging	0.71
IGL02100:St14	APN	9	31,011,426 (GRCm39)	splice site	probably benign
IGL02494:St14	APN	9	31,019,941 (GRCm39)	missense	possibly damaging	0.47
IGL02588:St14	APN	9	31,001,329 (GRCm39)	splice site	probably benign
IGL02663:St14	APN	9	31,011,678 (GRCm39)	splice site	probably null
IGL02711:St14	APN	9	31,001,196 (GRCm39)	missense	probably benign	0.05
IGL03130:St14	APN	9	31,008,367 (GRCm39)	critical splice donor site	probably null
IGL03296:St14	APN	9	31,020,008 (GRCm39)	missense	probably damaging	0.98
IGL03400:St14	APN	9	31,008,267 (GRCm39)	splice site	probably benign
R0101:St14	UTSW	9	31,008,403 (GRCm39)	missense	probably benign	0.23
R0225:St14	UTSW	9	31,019,580 (GRCm39)	critical splice acceptor site	probably null
R0335:St14	UTSW	9	31,002,620 (GRCm39)	splice site	probably benign
R0892:St14	UTSW	9	31,011,724 (GRCm39)	missense	probably benign	0.38
R1334:St14	UTSW	9	31,019,506 (GRCm39)	missense	probably damaging	1.00
R1487:St14	UTSW	9	31,008,476 (GRCm39)	missense	probably damaging	1.00
R1521:St14	UTSW	9	31,019,511 (GRCm39)	missense	probably benign	0.03
R1782:St14	UTSW	9	31,011,460 (GRCm39)	missense	probably damaging	1.00
R1920:St14	UTSW	9	31,001,166 (GRCm39)	missense	possibly damaging	0.94
R1921:St14	UTSW	9	31,001,166 (GRCm39)	missense	possibly damaging	0.94
R1922:St14	UTSW	9	31,001,166 (GRCm39)	missense	possibly damaging	0.94
R1933:St14	UTSW	9	31,017,508 (GRCm39)	missense	probably benign	0.00
R2070:St14	UTSW	9	31,002,669 (GRCm39)	missense	probably damaging	1.00
R2411:St14	UTSW	9	31,019,530 (GRCm39)	missense	probably benign	0.13
R4152:St14	UTSW	9	31,001,802 (GRCm39)	missense	probably benign	0.08
R4375:St14	UTSW	9	31,001,754 (GRCm39)	missense	probably benign	0.02
R4419:St14	UTSW	9	31,008,224 (GRCm39)	missense	probably damaging	1.00
R4747:St14	UTSW	9	31,015,053 (GRCm39)	missense	possibly damaging	0.78
R4791:St14	UTSW	9	31,006,918 (GRCm39)	missense	probably benign	0.27
R5056:St14	UTSW	9	31,008,847 (GRCm39)	splice site	probably null
R5134:St14	UTSW	9	31,006,879 (GRCm39)	missense	probably benign	0.00
R5241:St14	UTSW	9	31,011,714 (GRCm39)	nonsense	probably null
R5325:St14	UTSW	9	31,008,274 (GRCm39)	splice site	probably null
R5644:St14	UTSW	9	31,017,806 (GRCm39)	missense	probably benign
R5828:St14	UTSW	9	31,002,803 (GRCm39)	missense	probably damaging	1.00
R5922:St14	UTSW	9	31,041,200 (GRCm39)	intron	probably benign
R5930:St14	UTSW	9	31,015,056 (GRCm39)	missense	probably damaging	1.00
R5963:St14	UTSW	9	31,017,853 (GRCm39)	intron	probably benign
R6911:St14	UTSW	9	31,018,081 (GRCm39)	missense	probably benign	0.00
R6937:St14	UTSW	9	31,040,956 (GRCm39)	splice site	probably null
R6986:St14	UTSW	9	31,007,845 (GRCm39)	missense	probably damaging	0.98
R7226:St14	UTSW	9	31,011,448 (GRCm39)	missense	possibly damaging	0.63
R7395:St14	UTSW	9	31,008,195 (GRCm39)	missense	probably benign	0.29
R7400:St14	UTSW	9	31,019,571 (GRCm39)	missense	probably benign	0.36
R8194:St14	UTSW	9	31,042,921 (GRCm39)	start codon destroyed	probably null	0.95
R8886:St14	UTSW	9	31,008,420 (GRCm39)	missense	possibly damaging	0.93
R9248:St14	UTSW	9	31,002,905 (GRCm39)	missense	probably damaging	1.00
R9440:St14	UTSW	9	31,007,845 (GRCm39)	missense	probably damaging	0.98
Z1177:St14	UTSW	9	31,001,803 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AATGGCTCAGAGACAGTGCAC -3'
(R):5'- CCATTGCACATGCTGGTTTC -3'

Sequencing Primer
(F):5'- AGTGCACTGGACCCTTCC -3'
(R):5'- ATGCTGGTTTCCCCAAGAAC -3'

Posted On

2016-04-15