Mutagenetix > Incidental Mutation

Incidental Mutation 'R5037:Zfp667'

389570

Institutional Source

Beutler Lab

Gene Symbol

Zfp667

Ensembl Gene

ENSMUSG00000054893

Gene Name

zinc finger protein 667

Synonyms

A830025F02Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5037 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

6289578-6310882 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 6308949 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Serine at position 539 (I539S)

Ref Sequence

ENSEMBL: ENSMUSP00000128658 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086327] [ENSMUST00000108562] [ENSMUST00000153840] [ENSMUST00000170776]

AlphaFold

Q2TL60

Predicted Effect

possibly damaging
Transcript: ENSMUST00000086327
AA Change: I539S

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000083507
Gene: ENSMUSG00000054893
AA Change: I539S

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108562
AA Change: I539S

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000104202
Gene: ENSMUSG00000054893
AA Change: I539S

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000153840

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170776
AA Change: I539S

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000128658
Gene: ENSMUSG00000054893
AA Change: I539S

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.3%

Validation Efficiency

Allele List at MGI

All alleles(4) : Targeted, other(2) Gene trapped(2)

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Btaf1	T	C	19: 36,980,931 (GRCm39)	V1584A	probably damaging	Het
Ccdc136	A	T	6: 29,417,122 (GRCm39)	S648C	probably damaging	Het
Ccna2	A	G	3: 36,625,152 (GRCm39)		probably benign	Het
Cdc23	C	A	18: 34,784,742 (GRCm39)	V7L	unknown	Het
Cenpf	T	C	1: 189,416,043 (GRCm39)	E94G	probably damaging	Het
Clic1	G	A	17: 35,274,235 (GRCm39)	V139I	probably benign	Het
Coasy	A	G	11: 100,975,648 (GRCm39)	E327G	probably damaging	Het
Col6a5	C	T	9: 105,805,337 (GRCm39)	E1190K	unknown	Het
Cyp2c70	A	G	19: 40,172,441 (GRCm39)	V67A	possibly damaging	Het
Dglucy	A	G	12: 100,801,500 (GRCm39)	S52G	probably benign	Het
Dync1h1	A	G	12: 110,607,341 (GRCm39)	N2644S	probably benign	Het
Eif4g2	A	T	7: 110,676,239 (GRCm39)	N347K	probably benign	Het
Epha10	T	C	4: 124,809,178 (GRCm39)		probably benign	Het
Epm2aip1	T	C	9: 111,101,218 (GRCm39)	F64L	probably benign	Het
Eri2	G	A	7: 119,384,897 (GRCm39)	L535F	probably benign	Het
Garin5b	T	G	7: 4,761,575 (GRCm39)	K379T	possibly damaging	Het
Gm17430	T	C	18: 9,726,561 (GRCm39)	E37G	probably benign	Het
Heatr5b	T	A	17: 79,131,939 (GRCm39)	Q388L	probably benign	Het
Htr1f	A	G	16: 64,746,291 (GRCm39)	W334R	probably damaging	Het
Icam4	A	T	9: 20,940,937 (GRCm39)	C717*	probably null	Het
Iqgap1	A	C	7: 80,383,848 (GRCm39)	L1072W	probably damaging	Het
Kbtbd11	G	T	8: 15,077,886 (GRCm39)	A162S	probably benign	Het
Kif13b	T	G	14: 64,996,038 (GRCm39)	Y941*	probably null	Het
Kndc1	T	C	7: 139,490,371 (GRCm39)	V291A	possibly damaging	Het
Lrrn3	A	T	12: 41,503,594 (GRCm39)	I241N	probably damaging	Het
Macf1	A	G	4: 123,349,312 (GRCm39)	S2387P	probably damaging	Het
Msh5	A	G	17: 35,251,369 (GRCm39)	L451S	possibly damaging	Het
Mthfd1	T	G	12: 76,340,914 (GRCm39)	F258V	probably damaging	Het
Ncstn	C	T	1: 171,896,193 (GRCm39)	R495H	probably damaging	Het
Or4k2	T	G	14: 50,423,745 (GRCm39)	T310P	probably benign	Het
Pkd1l3	T	A	8: 110,392,268 (GRCm39)	I1954N	probably damaging	Het
Ppox	C	A	1: 171,105,169 (GRCm39)	V340L	probably damaging	Het
Prkag1	T	C	15: 98,713,768 (GRCm39)	T21A	possibly damaging	Het
Pygo1	C	A	9: 72,852,199 (GRCm39)	H129N	probably damaging	Het
Rad9a	A	T	19: 4,247,173 (GRCm39)	C271S	probably benign	Het
Raph1	T	C	1: 60,535,381 (GRCm39)		probably null	Het
Reln	A	G	5: 22,153,510 (GRCm39)	F2265L	probably damaging	Het
Shc4	A	G	2: 125,471,647 (GRCm39)	I304T	probably damaging	Het
Slc35f3	T	A	8: 127,116,011 (GRCm39)	L313M	probably damaging	Het
Spata31g1	T	A	4: 42,972,195 (GRCm39)	H509Q	probably benign	Het
Spef1l	A	T	7: 139,558,587 (GRCm39)	S3R	possibly damaging	Het
Sycp2l	T	A	13: 41,283,337 (GRCm39)	M191K	possibly damaging	Het
Tmem132c	C	A	5: 127,630,199 (GRCm39)	Q579K	probably benign	Het
Trbj2-5	A	G	6: 41,520,394 (GRCm39)		probably benign	Het
Ttc38	A	G	15: 85,728,741 (GRCm39)	E231G	probably benign	Het
Utp20	A	G	10: 88,611,192 (GRCm39)	V1375A	probably benign	Het
Vcan	T	A	13: 89,852,096 (GRCm39)	T955S	probably damaging	Het
Vmn1r89	T	C	7: 12,953,314 (GRCm39)	C17R	possibly damaging	Het
Wnk1	A	G	6: 119,942,696 (GRCm39)		probably benign	Het
Zfp738	T	A	13: 67,818,320 (GRCm39)	H557L	probably damaging	Het

Other mutations in Zfp667

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00821:Zfp667	APN	7	6,308,396 (GRCm39)	missense	possibly damaging	0.53
IGL01325:Zfp667	APN	7	6,293,545 (GRCm39)	missense	probably damaging	1.00
IGL01386:Zfp667	APN	7	6,307,869 (GRCm39)	missense	probably benign	0.00
IGL01960:Zfp667	APN	7	6,308,336 (GRCm39)	missense	probably benign	0.00
IGL03394:Zfp667	APN	7	6,292,438 (GRCm39)	critical splice donor site	probably null
B5639:Zfp667	UTSW	7	6,293,544 (GRCm39)	missense	probably damaging	1.00
R0458:Zfp667	UTSW	7	6,307,844 (GRCm39)	missense	probably benign	0.40
R0845:Zfp667	UTSW	7	6,309,091 (GRCm39)	missense	possibly damaging	0.85
R1768:Zfp667	UTSW	7	6,308,066 (GRCm39)	missense	possibly damaging	0.53
R1953:Zfp667	UTSW	7	6,308,087 (GRCm39)	missense	probably benign	0.04
R2023:Zfp667	UTSW	7	6,308,416 (GRCm39)	missense	possibly damaging	0.85
R3159:Zfp667	UTSW	7	6,308,999 (GRCm39)	missense	probably damaging	1.00
R4080:Zfp667	UTSW	7	6,308,105 (GRCm39)	missense	possibly damaging	0.71
R4476:Zfp667	UTSW	7	6,307,598 (GRCm39)	missense	possibly damaging	0.53
R4584:Zfp667	UTSW	7	6,293,624 (GRCm39)	missense	possibly damaging	0.84
R4783:Zfp667	UTSW	7	6,308,684 (GRCm39)	missense	possibly damaging	0.83
R5300:Zfp667	UTSW	7	6,307,635 (GRCm39)	missense	probably benign
R5311:Zfp667	UTSW	7	6,308,715 (GRCm39)	missense	probably benign	0.10
R5312:Zfp667	UTSW	7	6,308,466 (GRCm39)	missense	probably benign
R5340:Zfp667	UTSW	7	6,308,252 (GRCm39)	missense	possibly damaging	0.53
R6262:Zfp667	UTSW	7	6,307,973 (GRCm39)	missense	probably benign	0.03
R7386:Zfp667	UTSW	7	6,308,949 (GRCm39)	missense	possibly damaging	0.86
R8383:Zfp667	UTSW	7	6,308,370 (GRCm39)	missense	probably damaging	0.98
R8919:Zfp667	UTSW	7	6,308,256 (GRCm39)	missense	possibly damaging	0.53
R9099:Zfp667	UTSW	7	6,308,322 (GRCm39)	missense	probably benign	0.00
R9422:Zfp667	UTSW	7	6,308,321 (GRCm39)	missense	probably benign	0.00
Z1177:Zfp667	UTSW	7	6,307,856 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GGTGTGTAGTCGCCTTTCATCC -3'
(R):5'- CCGAATGTATACTCTGATGTCGAATC -3'

Sequencing Primer
(F):5'- CTCATTGCATGCAAGGAATGTG -3'
(R):5'- TGATGTCGAATCAGCGATGAGC -3'

Posted On

2016-06-06