Mutagenetix > Incidental Mutation

Incidental Mutation 'R0498:Or4k15'

40627

Institutional Source

Beutler Lab

Gene Symbol

Or4k15

Ensembl Gene

ENSMUSG00000059488

Gene Name

olfactory receptor family 4 subfamily K member 15

Synonyms

Olfr727, GA_x6K02T2PMLR-5817082-5818056, MOR246-2

MMRRC Submission

038694-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.140) question?

Stock #

R0498 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50364010-50365044 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 50364750 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 239 (T239P)

Ref Sequence

ENSEMBL: ENSMUSP00000149886 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079142] [ENSMUST00000215317]

AlphaFold

A2RTN7

Predicted Effect

probably damaging
Transcript: ENSMUST00000079142
AA Change: T239P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078145
Gene: ENSMUSG00000059488
AA Change: T239P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	8.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	36	290	1.5e-7	PFAM
Pfam:7tm_1	41	287	1.1e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205947

Predicted Effect

probably damaging
Transcript: ENSMUST00000215317
AA Change: T239P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6333

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 96.9%
20x: 94.4%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	A	T	7: 40,642,718 (GRCm39)	D220V	probably benign	Het
Adgrf2	A	G	17: 43,025,206 (GRCm39)		probably benign	Het
Aldh18a1	A	G	19: 40,562,716 (GRCm39)	V219A	probably benign	Het
Anapc10	A	G	8: 80,501,610 (GRCm39)	D126G	probably benign	Het
Ap1m2	T	C	9: 21,207,129 (GRCm39)	*426W	probably null	Het
Arhgap21	A	G	2: 20,867,928 (GRCm39)	I865T	probably damaging	Het
Armc8	A	G	9: 99,379,345 (GRCm39)	V527A	probably damaging	Het
Asic5	A	T	3: 81,913,778 (GRCm39)		probably benign	Het
Baz2b	A	C	2: 59,732,340 (GRCm39)		probably benign	Het
Bpifa5	T	C	2: 154,009,169 (GRCm39)	V237A	probably damaging	Het
Brip1	T	A	11: 86,088,745 (GRCm39)	K52I	possibly damaging	Het
Cacna1g	T	C	11: 94,350,685 (GRCm39)	I387V	probably damaging	Het
Cbr4	A	G	8: 61,948,107 (GRCm39)	I135V	probably benign	Het
Ccdc66	C	T	14: 27,222,197 (GRCm39)		probably null	Het
Cubn	G	A	2: 13,449,078 (GRCm39)	T999M	probably damaging	Het
Dnai3	G	T	3: 145,787,119 (GRCm39)	D305E	possibly damaging	Het
Dpp8	C	T	9: 64,953,077 (GRCm39)		probably benign	Het
Dsg1b	T	C	18: 20,542,390 (GRCm39)	S966P	possibly damaging	Het
Erp27	T	C	6: 136,896,862 (GRCm39)		probably benign	Het
Fat4	A	T	3: 39,034,786 (GRCm39)	I2813L	probably benign	Het
Fhod1	G	A	8: 106,056,488 (GRCm39)	R1101C	probably damaging	Het
Hoxc9	T	C	15: 102,892,359 (GRCm39)	S191P	probably damaging	Het
Izumo4	T	C	10: 80,540,030 (GRCm39)		probably null	Het
Kalrn	C	T	16: 33,875,261 (GRCm39)	D104N	possibly damaging	Het
Kank4	A	T	4: 98,667,873 (GRCm39)	D191E	probably benign	Het
Kbtbd11	A	G	8: 15,077,605 (GRCm39)	E68G	probably benign	Het
Kdr	C	T	5: 76,119,798 (GRCm39)	V654I	probably benign	Het
Klra1	A	T	6: 130,349,782 (GRCm39)		probably null	Het
Kmt2e	T	A	5: 23,683,970 (GRCm39)	Y373*	probably null	Het
Lepr	A	T	4: 101,602,889 (GRCm39)	M226L	probably benign	Het
Lrp1b	T	A	2: 41,348,417 (GRCm39)	I800F	probably benign	Het
Lta4h	T	C	10: 93,307,833 (GRCm39)		probably benign	Het
Map3k7	T	C	4: 31,974,814 (GRCm39)		probably benign	Het
Map4k4	G	A	1: 40,029,338 (GRCm39)	R371Q	probably benign	Het
Mgat4f	A	G	1: 134,318,610 (GRCm39)	I461V	possibly damaging	Het
Mme	A	G	3: 63,253,487 (GRCm39)	I444V	probably damaging	Het
Mms19	C	T	19: 41,938,212 (GRCm39)	R582Q	possibly damaging	Het
Mtss1	A	G	15: 58,817,286 (GRCm39)	S502P	probably damaging	Het
Myo3a	G	T	2: 22,467,441 (GRCm39)	A232S	possibly damaging	Het
Nwd2	G	T	5: 63,963,686 (GRCm39)	W1090L	probably damaging	Het
Or8b12	G	A	9: 37,657,550 (GRCm39)	G40E	probably damaging	Het
Pcm1	G	A	8: 41,746,806 (GRCm39)	S1335N	probably benign	Het
Pdzph1	A	G	17: 59,280,825 (GRCm39)	F486L	probably benign	Het
Piezo2	T	C	18: 63,235,245 (GRCm39)	K552R	possibly damaging	Het
Plekhs1	T	A	19: 56,469,536 (GRCm39)		probably null	Het
Pprc1	C	T	19: 46,060,007 (GRCm39)	Q1514*	probably null	Het
Ralgapa1	T	C	12: 55,736,576 (GRCm39)	T1831A	possibly damaging	Het
Rnpep	G	T	1: 135,193,090 (GRCm39)	D455E	probably damaging	Het
Rpgrip1	T	A	14: 52,368,771 (GRCm39)		probably benign	Het
Saxo1	A	T	4: 86,397,133 (GRCm39)	M135K	possibly damaging	Het
Serpina12	T	C	12: 104,002,048 (GRCm39)	T223A	probably damaging	Het
Serpinb3a	A	G	1: 106,974,880 (GRCm39)	F218L	probably damaging	Het
Serpinb9f	T	G	13: 33,509,990 (GRCm39)		probably benign	Het
Spata33	A	G	8: 123,948,662 (GRCm39)	D98G	probably benign	Het
Stard13	T	A	5: 150,975,942 (GRCm39)	Y742F	probably damaging	Het
Tecta	A	G	9: 42,288,910 (GRCm39)	Y552H	probably damaging	Het
Tie1	A	T	4: 118,336,358 (GRCm39)		probably benign	Het
Tmem161a	A	G	8: 70,633,623 (GRCm39)	T254A	probably benign	Het
Tmem30a	G	T	9: 79,681,376 (GRCm39)	Y264*	probably null	Het
Tmem87a	A	T	2: 120,224,946 (GRCm39)	I105K	probably benign	Het
Tnrc6b	A	T	15: 80,742,920 (GRCm39)	D51V	probably damaging	Het
Trgc3	T	A	13: 19,445,262 (GRCm39)	M70K	probably damaging	Het
Trpc4	T	C	3: 54,198,632 (GRCm39)	F519L	probably damaging	Het
Ttn	T	C	2: 76,539,925 (GRCm39)	T26027A	probably damaging	Het
Vmn1r198	A	C	13: 22,539,144 (GRCm39)	H121P	probably damaging	Het
Vps33a	A	G	5: 123,709,024 (GRCm39)	F64L	probably benign	Het
Zfp1008	T	C	13: 62,755,201 (GRCm39)	N39S	probably damaging	Het
Zfp994	A	T	17: 22,419,882 (GRCm39)	C356S	probably damaging	Het

Other mutations in Or4k15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00906:Or4k15	APN	14	50,364,214 (GRCm39)	missense	probably damaging	1.00
IGL01306:Or4k15	APN	14	50,364,039 (GRCm39)	missense	probably benign	0.00
ANU23:Or4k15	UTSW	14	50,364,039 (GRCm39)	missense	probably benign	0.00
R0574:Or4k15	UTSW	14	50,364,139 (GRCm39)	missense	probably damaging	1.00
R1201:Or4k15	UTSW	14	50,364,813 (GRCm39)	missense	probably damaging	1.00
R2112:Or4k15	UTSW	14	50,364,080 (GRCm39)	missense	probably damaging	1.00
R2435:Or4k15	UTSW	14	50,364,211 (GRCm39)	missense	probably damaging	1.00
R4238:Or4k15	UTSW	14	50,364,889 (GRCm39)	missense	probably benign
R4611:Or4k15	UTSW	14	50,364,530 (GRCm39)	missense	probably benign	0.12
R4663:Or4k15	UTSW	14	50,364,939 (GRCm39)	missense	probably benign	0.00
R4672:Or4k15	UTSW	14	50,364,714 (GRCm39)	missense	probably benign	0.02
R5022:Or4k15	UTSW	14	50,364,469 (GRCm39)	missense	possibly damaging	0.78
R5062:Or4k15	UTSW	14	50,364,894 (GRCm39)	missense	probably damaging	1.00
R5924:Or4k15	UTSW	14	50,364,139 (GRCm39)	missense	probably damaging	1.00
R6702:Or4k15	UTSW	14	50,364,688 (GRCm39)	missense	probably damaging	1.00
R6703:Or4k15	UTSW	14	50,364,688 (GRCm39)	missense	probably damaging	1.00
R7497:Or4k15	UTSW	14	50,364,952 (GRCm39)	missense	probably benign	0.20
R7615:Or4k15	UTSW	14	50,364,446 (GRCm39)	missense	probably benign	0.07
R7798:Or4k15	UTSW	14	50,364,895 (GRCm39)	missense	probably damaging	1.00
R8413:Or4k15	UTSW	14	50,364,827 (GRCm39)	missense	probably benign	0.19
R8439:Or4k15	UTSW	14	50,364,604 (GRCm39)	missense	probably benign
R8841:Or4k15	UTSW	14	50,364,666 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCAATATGCAAGCCTCTCCACTAC -3'
(R):5'- AGATAGCGACCCCTCAGCTTTGAC -3'

Sequencing Primer
(F):5'- TGACAATCATGAGTCGCCG -3'
(R):5'- TCAGCTTTGACATTGCTGC -3'

Posted On

2013-05-23