Mutagenetix > Incidental Mutation

Incidental Mutation 'R5308:Or1j16'

434457

Institutional Source

Beutler Lab

Gene Symbol

Or1j16

Ensembl Gene

ENSMUSG00000059251

Gene Name

olfactory receptor family 1 subfamily J member 16

Synonyms

Olfr345, MOR136-7, GA_x6K02T2NLDC-33334179-33335114

MMRRC Submission

042891-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R5308 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

36530053-36530988 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 36530706 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 218 (Y218*)

Ref Sequence

ENSEMBL: ENSMUSP00000076122 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076850]

AlphaFold

Q8VGK4

Predicted Effect

probably null
Transcript: ENSMUST00000076850
AA Change: Y218*

SMART Domains

Protein: ENSMUSP00000076122
Gene: ENSMUSG00000059251
AA Change: Y218*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.6e-57	PFAM
Pfam:7tm_1	41	290	2.6e-22	PFAM

Coding Region Coverage

1x: 99.2%
3x: 97.7%
10x: 95.4%
20x: 91.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	C	G	3: 124,199,999 (GRCm39)	G531A	probably damaging	Het
4921504E06Rik	T	C	2: 19,528,892 (GRCm39)	D163G	probably damaging	Het
Abcd4	C	T	12: 84,650,067 (GRCm39)		probably null	Het
Alg9	T	C	9: 50,734,011 (GRCm39)	S570P	possibly damaging	Het
Angptl3	C	A	4: 98,922,723 (GRCm39)	H255N	probably benign	Het
Ank3	G	A	10: 69,838,395 (GRCm39)	R1566K	possibly damaging	Het
Cdc37	A	T	9: 21,052,060 (GRCm39)	D326E	probably benign	Het
Cdk13	T	C	13: 17,946,898 (GRCm39)	K6R	probably damaging	Het
Ces1b	T	C	8: 93,793,645 (GRCm39)	K315E	probably benign	Het
Cfap61	G	A	2: 145,951,908 (GRCm39)	G190S	probably damaging	Het
Cimap3	T	C	3: 105,908,419 (GRCm39)	T107A	probably benign	Het
Ckap4	T	C	10: 84,364,238 (GRCm39)	E275G	probably benign	Het
Cplane1	G	A	15: 8,290,174 (GRCm39)		probably null	Het
Csf3r	A	G	4: 125,929,137 (GRCm39)	D349G	probably benign	Het
Cyp2d37-ps	T	C	15: 82,574,012 (GRCm39)		noncoding transcript	Het
Dmbt1	T	A	7: 130,642,751 (GRCm39)	C190S	probably damaging	Het
Dnah11	A	G	12: 118,049,415 (GRCm39)	F1670L	possibly damaging	Het
Dnah5	A	T	15: 28,229,797 (GRCm39)	I144F	possibly damaging	Het
Eno2	C	A	6: 124,744,056 (GRCm39)	V84L	probably damaging	Het
Ercc4	G	T	16: 12,948,028 (GRCm39)	R325L	probably damaging	Het
Fcgr2b	T	G	1: 170,793,279 (GRCm39)	Q250P	probably benign	Het
Garin5a	T	C	7: 44,149,606 (GRCm39)	V109A	probably damaging	Het
Garre1	T	A	7: 33,945,180 (GRCm39)	K355*	probably null	Het
Gcfc2	T	C	6: 81,920,524 (GRCm39)		probably null	Het
Glb1l2	C	T	9: 26,676,055 (GRCm39)	G509D	probably damaging	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Grn	A	G	11: 102,327,018 (GRCm39)	N160D	possibly damaging	Het
Hexd	T	A	11: 121,113,095 (GRCm39)	V510D	probably damaging	Het
Igfbp1	G	A	11: 7,149,919 (GRCm39)		probably null	Het
Itga11	T	A	9: 62,663,051 (GRCm39)	M589K	probably benign	Het
Itpr1	A	T	6: 108,333,472 (GRCm39)	S51C	probably damaging	Het
Klra3	T	C	6: 130,311,270 (GRCm39)		probably null	Het
Mad2l1	T	C	6: 66,514,675 (GRCm39)		probably null	Het
Matn3	CGGGGCTCGGGGGC	CGGGGC	12: 9,002,308 (GRCm39)		probably null	Het
Myo9a	T	A	9: 59,771,244 (GRCm39)	Y939N	probably damaging	Het
Nxpe3	C	T	16: 55,686,834 (GRCm39)	S58N	probably benign	Het
Or3a1c	T	A	11: 74,046,397 (GRCm39)	M139K	probably damaging	Het
Or4c103	T	C	2: 88,513,749 (GRCm39)	E109G	probably benign	Het
Or4k45	C	T	2: 111,394,899 (GRCm39)	A297T	probably damaging	Het
Paics	T	C	5: 77,104,479 (GRCm39)	S35P	probably damaging	Het
Pcnt	A	C	10: 76,192,159 (GRCm39)	Y2717*	probably null	Het
Plekho2	T	A	9: 65,465,957 (GRCm39)	N144Y	probably damaging	Het
Plscr5	T	C	9: 92,080,565 (GRCm39)	F17S	possibly damaging	Het
Prrc2a	C	T	17: 35,380,023 (GRCm39)	R192H	unknown	Het
Rbm27	T	A	18: 42,460,275 (GRCm39)	M735K	probably damaging	Het
Rfc1	T	A	5: 65,436,804 (GRCm39)	K625N	probably damaging	Het
Ric8b	T	A	10: 84,783,611 (GRCm39)	F156L	probably benign	Het
Romo1	C	A	2: 155,986,473 (GRCm39)	A32E	possibly damaging	Het
Rpl26	T	A	11: 68,795,284 (GRCm39)	Y135N	probably damaging	Het
Sacs	T	C	14: 61,429,849 (GRCm39)	V636A	probably benign	Het
Scyl2	A	T	10: 89,477,869 (GRCm39)	I710N	probably benign	Het
Sh3bp5	C	A	14: 31,099,452 (GRCm39)	R265L	probably benign	Het
Slc4a1	T	A	11: 102,249,903 (GRCm39)	I154F	probably damaging	Het
Snx11	A	G	11: 96,661,535 (GRCm39)	S157P	probably damaging	Het
Snx18	G	A	13: 113,753,383 (GRCm39)	Q517*	probably null	Het
Stard4	G	T	18: 33,336,678 (GRCm39)	N212K	probably damaging	Het
Strn3	A	G	12: 51,676,168 (GRCm39)	Y454H	probably damaging	Het
Stx17	A	T	4: 48,182,851 (GRCm39)		probably benign	Het
Tas2r121	G	A	6: 132,677,480 (GRCm39)	T164I	possibly damaging	Het
Tbc1d8	T	C	1: 39,428,490 (GRCm39)	Y485C	probably damaging	Het
Tmem45b	T	C	9: 31,340,380 (GRCm39)	M8V	probably damaging	Het
Usp28	T	C	9: 48,948,501 (GRCm39)	F844L	probably damaging	Het
Usp32	T	A	11: 84,908,544 (GRCm39)	N1054I	probably benign	Het
Xpa	T	A	4: 46,185,659 (GRCm39)	E106D	probably benign	Het
Zfp85	C	T	13: 67,896,974 (GRCm39)	C366Y	probably damaging	Het

Other mutations in Or1j16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03323:Or1j16	APN	2	36,530,153 (GRCm39)	missense	possibly damaging	0.95
IGL03399:Or1j16	APN	2	36,530,894 (GRCm39)	missense	possibly damaging	0.78
R1164:Or1j16	UTSW	2	36,530,132 (GRCm39)	missense	probably benign
R1878:Or1j16	UTSW	2	36,530,201 (GRCm39)	missense	possibly damaging	0.83
R1879:Or1j16	UTSW	2	36,530,201 (GRCm39)	missense	possibly damaging	0.83
R1954:Or1j16	UTSW	2	36,530,227 (GRCm39)	missense	possibly damaging	0.86
R2206:Or1j16	UTSW	2	36,530,201 (GRCm39)	missense	possibly damaging	0.83
R2207:Or1j16	UTSW	2	36,530,201 (GRCm39)	missense	possibly damaging	0.83
R3773:Or1j16	UTSW	2	36,530,333 (GRCm39)	missense	probably benign	0.16
R4470:Or1j16	UTSW	2	36,530,233 (GRCm39)	missense	probably damaging	1.00
R4583:Or1j16	UTSW	2	36,530,626 (GRCm39)	missense	probably damaging	1.00
R4744:Or1j16	UTSW	2	36,530,991 (GRCm39)	splice site	probably null
R4897:Or1j16	UTSW	2	36,530,906 (GRCm39)	missense	probably damaging	1.00
R5510:Or1j16	UTSW	2	36,530,975 (GRCm39)	missense	probably benign
R6175:Or1j16	UTSW	2	36,530,063 (GRCm39)	missense	probably benign	0.00
R7736:Or1j16	UTSW	2	36,530,197 (GRCm39)	missense	probably damaging	1.00
R7737:Or1j16	UTSW	2	36,530,632 (GRCm39)	missense	probably benign	0.01
R9783:Or1j16	UTSW	2	36,530,161 (GRCm39)	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- GGCCTTATCCATTGCCAATG -3'
(R):5'- GGTGTGACCACAGTGTACATC -3'

Sequencing Primer
(F):5'- AATGCCCTTGTGCGCAC -3'
(R):5'- GTGTACATCACAGCCACAATGG -3'

Posted On

2016-10-06