Mutagenetix > Incidental Mutation

Incidental Mutation 'R6442:Vmn2r67'

519059

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r67

Ensembl Gene

ENSMUSG00000095664

Gene Name

vomeronasal 2, receptor 67

Synonyms

EG620672

MMRRC Submission

044580-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R6442 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

84785448-84805110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 84805046 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 22 (D22V)

Ref Sequence

ENSEMBL: ENSMUSP00000126007 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168730]

AlphaFold

K7N6T2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000168730
AA Change: D22V

PolyPhen 2 Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000126007
Gene: ENSMUSG00000095664
AA Change: D22V

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:ANF_receptor	77	464	2.1e-31	PFAM
Pfam:NCD3G	507	559	4.8e-19	PFAM
Pfam:7tm_3	590	827	1.4e-53	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 93.9%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atp9a	T	C	2: 168,491,481 (GRCm39)	T695A	probably benign	Het
Btla	A	G	16: 45,044,821 (GRCm39)	N36D	probably benign	Het
Btla	T	C	16: 45,070,713 (GRCm39)	V224A	possibly damaging	Het
Cep126	T	C	9: 8,100,564 (GRCm39)	N657D	probably benign	Het
Cops3	T	A	11: 59,718,780 (GRCm39)	K171N	probably benign	Het
Crocc2	A	G	1: 93,112,775 (GRCm39)	R193G	probably benign	Het
Dis3l	T	C	9: 64,214,837 (GRCm39)	I911V	probably benign	Het
Dpp6	G	A	5: 27,923,507 (GRCm39)		probably null	Het
Efcab5	A	T	11: 76,996,260 (GRCm39)	Y1100*	probably null	Het
Fcgbpl1	C	T	7: 27,843,611 (GRCm39)	T833I	possibly damaging	Het
Galnt10	A	G	11: 57,656,448 (GRCm39)	T211A	probably benign	Het
Gdf9	A	G	11: 53,324,515 (GRCm39)	T95A	probably benign	Het
Gm826	T	A	2: 160,169,328 (GRCm39)		probably benign	Het
Hsd17b6	C	T	10: 127,829,636 (GRCm39)		probably null	Het
Hyal4	A	G	6: 24,765,849 (GRCm39)	N401S	probably benign	Het
Itga8	C	T	2: 12,234,954 (GRCm39)	V435I	probably benign	Het
Nr1h5	G	A	3: 102,848,427 (GRCm39)	P418L	probably damaging	Het
Or3a4	G	A	11: 73,945,505 (GRCm39)	R27W	probably benign	Het
Or4f15	G	A	2: 111,813,874 (GRCm39)	P182S	probably damaging	Het
Or4n4	T	G	14: 50,518,826 (GRCm39)	K295Q	probably damaging	Het
Or6c214	C	A	10: 129,591,277 (GRCm39)	G14V	probably damaging	Het
Or9i14	T	C	19: 13,792,992 (GRCm39)		probably benign	Het
Prdm11	G	A	2: 92,805,990 (GRCm39)	A320V	probably benign	Het
Ptpn5	T	C	7: 46,732,831 (GRCm39)		probably null	Het
Reln	T	A	5: 22,137,774 (GRCm39)	I2473L	probably benign	Het
Rmnd5a	A	T	6: 71,371,659 (GRCm39)	C180*	probably null	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Sapcd2	T	A	2: 25,266,134 (GRCm39)		probably benign	Het
Sec24b	A	G	3: 129,790,350 (GRCm39)	L462S	probably damaging	Het
Slc26a9	A	T	1: 131,686,555 (GRCm39)	Y425F	possibly damaging	Het
Sult1b1	T	C	5: 87,682,912 (GRCm39)	D11G	probably benign	Het
Tgs1	T	A	4: 3,604,760 (GRCm39)	Y727*	probably null	Het
Tmem80	T	C	7: 140,915,839 (GRCm39)	V83A	probably benign	Het
Ttn	T	C	2: 76,551,978 (GRCm39)	T31220A	probably benign	Het
Usp46	A	T	5: 74,177,377 (GRCm39)	D167E	probably benign	Het
Zswim5	C	A	4: 116,808,202 (GRCm39)	P262Q	probably damaging	Het

Other mutations in Vmn2r67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00096:Vmn2r67	APN	7	84,801,138 (GRCm39)	missense	probably damaging	1.00
IGL01346:Vmn2r67	APN	7	84,786,127 (GRCm39)	missense	probably damaging	1.00
IGL01373:Vmn2r67	APN	7	84,785,834 (GRCm39)	missense	probably benign	0.10
IGL01674:Vmn2r67	APN	7	84,785,651 (GRCm39)	missense	probably damaging	1.00
IGL01978:Vmn2r67	APN	7	84,800,649 (GRCm39)	critical splice donor site	probably null
IGL02013:Vmn2r67	APN	7	84,800,863 (GRCm39)	missense	probably benign	0.09
IGL02115:Vmn2r67	APN	7	84,800,787 (GRCm39)	missense	probably damaging	0.99
IGL02250:Vmn2r67	APN	7	84,805,008 (GRCm39)	missense	probably benign
IGL02252:Vmn2r67	APN	7	84,805,008 (GRCm39)	missense	probably benign
IGL02328:Vmn2r67	APN	7	84,799,898 (GRCm39)	missense	probably benign	0.41
IGL02740:Vmn2r67	APN	7	84,785,818 (GRCm39)	missense	probably damaging	1.00
IGL02940:Vmn2r67	APN	7	84,785,951 (GRCm39)	missense	probably benign	0.07
IGL03237:Vmn2r67	APN	7	84,799,118 (GRCm39)	missense	probably damaging	1.00
R0512:Vmn2r67	UTSW	7	84,799,900 (GRCm39)	missense	probably damaging	1.00
R1029:Vmn2r67	UTSW	7	84,785,974 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r67	UTSW	7	84,800,653 (GRCm39)	missense	probably damaging	0.98
R1282:Vmn2r67	UTSW	7	84,785,932 (GRCm39)	missense	probably benign
R1416:Vmn2r67	UTSW	7	84,800,824 (GRCm39)	missense	probably benign	0.06
R1429:Vmn2r67	UTSW	7	84,802,031 (GRCm39)	missense	possibly damaging	0.65
R1462:Vmn2r67	UTSW	7	84,805,046 (GRCm39)	missense	probably benign	0.00
R1462:Vmn2r67	UTSW	7	84,805,046 (GRCm39)	missense	probably benign	0.00
R1970:Vmn2r67	UTSW	7	84,801,013 (GRCm39)	missense	probably benign
R2229:Vmn2r67	UTSW	7	84,801,250 (GRCm39)	missense	probably benign	0.21
R2246:Vmn2r67	UTSW	7	84,785,764 (GRCm39)	missense	probably damaging	1.00
R2262:Vmn2r67	UTSW	7	84,786,182 (GRCm39)	missense	probably damaging	0.96
R2398:Vmn2r67	UTSW	7	84,785,921 (GRCm39)	missense	probably damaging	1.00
R4249:Vmn2r67	UTSW	7	84,799,722 (GRCm39)	splice site	probably null
R4666:Vmn2r67	UTSW	7	84,799,831 (GRCm39)	missense	probably benign
R4669:Vmn2r67	UTSW	7	84,799,732 (GRCm39)	missense	probably benign	0.11
R4966:Vmn2r67	UTSW	7	84,785,593 (GRCm39)	missense	probably damaging	1.00
R5264:Vmn2r67	UTSW	7	84,801,453 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r67	UTSW	7	84,786,230 (GRCm39)	missense	probably damaging	1.00
R5327:Vmn2r67	UTSW	7	84,785,698 (GRCm39)	missense	probably damaging	1.00
R5401:Vmn2r67	UTSW	7	84,785,765 (GRCm39)	missense	probably damaging	1.00
R5510:Vmn2r67	UTSW	7	84,801,023 (GRCm39)	missense	probably benign	0.39
R5574:Vmn2r67	UTSW	7	84,801,099 (GRCm39)	missense	probably benign	0.00
R5643:Vmn2r67	UTSW	7	84,799,151 (GRCm39)	nonsense	probably null
R5914:Vmn2r67	UTSW	7	84,801,044 (GRCm39)	missense	probably damaging	1.00
R6248:Vmn2r67	UTSW	7	84,799,768 (GRCm39)	missense	probably damaging	0.99
R6291:Vmn2r67	UTSW	7	84,799,142 (GRCm39)	missense	possibly damaging	0.88
R6309:Vmn2r67	UTSW	7	84,801,124 (GRCm39)	missense	probably benign
R6665:Vmn2r67	UTSW	7	84,785,900 (GRCm39)	missense	probably benign	0.07
R6701:Vmn2r67	UTSW	7	84,802,023 (GRCm39)	missense	probably damaging	1.00
R6848:Vmn2r67	UTSW	7	84,801,840 (GRCm39)	missense	probably benign	0.00
R6852:Vmn2r67	UTSW	7	84,801,361 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r67	UTSW	7	84,804,953 (GRCm39)	missense	possibly damaging	0.55
R7143:Vmn2r67	UTSW	7	84,801,846 (GRCm39)	missense	probably benign
R7197:Vmn2r67	UTSW	7	84,785,774 (GRCm39)	missense	possibly damaging	0.77
R7393:Vmn2r67	UTSW	7	84,805,086 (GRCm39)	missense	probably null	0.87
R7420:Vmn2r67	UTSW	7	84,785,944 (GRCm39)	missense	possibly damaging	0.52
R7622:Vmn2r67	UTSW	7	84,785,662 (GRCm39)	missense	probably damaging	1.00
R7664:Vmn2r67	UTSW	7	84,805,019 (GRCm39)	missense	probably benign	0.21
R7665:Vmn2r67	UTSW	7	84,801,196 (GRCm39)	nonsense	probably null
R7896:Vmn2r67	UTSW	7	84,785,920 (GRCm39)	missense	probably damaging	1.00
R7913:Vmn2r67	UTSW	7	84,801,036 (GRCm39)	missense	possibly damaging	0.87
R8026:Vmn2r67	UTSW	7	84,785,924 (GRCm39)	missense	probably damaging	1.00
R8114:Vmn2r67	UTSW	7	84,805,097 (GRCm39)	missense	probably benign	0.01
R8317:Vmn2r67	UTSW	7	84,785,834 (GRCm39)	missense	probably benign	0.10
R8363:Vmn2r67	UTSW	7	84,804,969 (GRCm39)	missense	probably benign	0.00
R8421:Vmn2r67	UTSW	7	84,785,893 (GRCm39)	missense	probably damaging	0.98
R8444:Vmn2r67	UTSW	7	84,785,854 (GRCm39)	missense	probably benign	0.01
R8751:Vmn2r67	UTSW	7	84,801,450 (GRCm39)	missense	probably benign	0.01
R8810:Vmn2r67	UTSW	7	84,786,346 (GRCm39)	missense	probably damaging	1.00
R8811:Vmn2r67	UTSW	7	84,799,895 (GRCm39)	missense	probably damaging	0.98
R9215:Vmn2r67	UTSW	7	84,802,008 (GRCm39)	missense	probably benign	0.00
R9342:Vmn2r67	UTSW	7	84,785,788 (GRCm39)	missense	probably benign	0.00
R9433:Vmn2r67	UTSW	7	84,804,917 (GRCm39)	missense	possibly damaging	0.60
R9453:Vmn2r67	UTSW	7	84,800,697 (GRCm39)	missense	probably benign	0.32
R9471:Vmn2r67	UTSW	7	84,799,723 (GRCm39)	critical splice donor site	probably null
R9526:Vmn2r67	UTSW	7	84,785,834 (GRCm39)	missense	probably benign	0.10
R9538:Vmn2r67	UTSW	7	84,801,327 (GRCm39)	missense
R9544:Vmn2r67	UTSW	7	84,786,317 (GRCm39)	missense	possibly damaging	0.53
R9574:Vmn2r67	UTSW	7	84,786,017 (GRCm39)	missense	probably benign	0.00
R9599:Vmn2r67	UTSW	7	84,804,941 (GRCm39)	missense	probably damaging	0.96
R9768:Vmn2r67	UTSW	7	84,802,037 (GRCm39)	missense	probably benign	0.23

Predicted Primers

PCR Primer
(F):5'- GCCAGAATTCACATAGTATCATGG -3'
(R):5'- GTAATAAATAGTGGTGCCTGGATAG -3'

Sequencing Primer
(F):5'- AGAATTCACATAGTATCATGGTTCAG -3'
(R):5'- AAATAGTGGTGCCTGGATAGGTTCAC -3'

Posted On

2018-05-24