Mutagenetix > Incidental Mutation

Incidental Mutation 'R7041:Zfp429'

547073

Institutional Source

Beutler Lab

Gene Symbol

Zfp429

Ensembl Gene

ENSMUSG00000078994

Gene Name

zinc finger protein 429

Synonyms

2810487A22Rik

MMRRC Submission

045140-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R7041 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67536024-67547938 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 67538830 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 205 (C205S)

Ref Sequence

ENSEMBL: ENSMUSP00000105354 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109732] [ENSMUST00000181071] [ENSMUST00000224684] [ENSMUST00000224825]

AlphaFold

Q7M6Y0

Predicted Effect

probably damaging
Transcript: ENSMUST00000109732
AA Change: C205S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000105354
Gene: ENSMUSG00000078994
AA Change: C205S

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART
ZnF_C2H2	119	141	5.12e1	SMART
ZnF_C2H2	147	169	2.27e-4	SMART
ZnF_C2H2	175	197	1.28e-3	SMART
ZnF_C2H2	203	225	1.56e-2	SMART
ZnF_C2H2	259	281	4.62e1	SMART
ZnF_C2H2	287	309	5.14e-3	SMART
ZnF_C2H2	315	337	6.78e-3	SMART
ZnF_C2H2	343	365	3.11e-2	SMART
ZnF_C2H2	371	393	1.25e-1	SMART
ZnF_C2H2	399	421	6.32e-3	SMART
ZnF_C2H2	427	449	1.47e-3	SMART
ZnF_C2H2	455	477	5.42e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181071

SMART Domains

Protein: ENSMUSP00000137755
Gene: ENSMUSG00000078994

Domain	Start	End	E-Value	Type
KRAB	15	75	7.16e-34	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000224684

Predicted Effect

probably benign
Transcript: ENSMUST00000224825

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

96% (53/55)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	G	T	7: 78,748,096 (GRCm39)	E956*	probably null	Het
Adam25	A	T	8: 41,207,121 (GRCm39)	H129L	probably benign	Het
Adgrl4	A	T	3: 151,144,959 (GRCm39)	H36L	probably benign	Het
Ago1	T	A	4: 126,357,499 (GRCm39)	I59F	possibly damaging	Het
Anapc1	A	T	2: 128,470,576 (GRCm39)	V1518E	possibly damaging	Het
Atxn1	A	G	13: 45,720,311 (GRCm39)	I528T	probably damaging	Het
B4galnt4	A	G	7: 140,650,593 (GRCm39)	H820R	probably damaging	Het
Cacna1h	T	C	17: 25,612,977 (GRCm39)	E282G	probably damaging	Het
Camk1	T	A	6: 113,316,475 (GRCm39)	M95L	probably benign	Het
Capn7	C	T	14: 31,058,642 (GRCm39)		probably benign	Het
Cav1	A	G	6: 17,339,143 (GRCm39)	E45G	possibly damaging	Het
Ccdc183	T	G	2: 25,503,682 (GRCm39)	E185A	probably benign	Het
Ccl2	T	A	11: 81,926,489 (GRCm39)	M1K	probably null	Het
Cep97	T	A	16: 55,726,117 (GRCm39)	H590L	probably benign	Het
Dsg1c	A	T	18: 20,399,201 (GRCm39)	I102F	probably damaging	Het
Fcho2	A	G	13: 98,921,334 (GRCm39)	Y184H	possibly damaging	Het
Gart	C	T	16: 91,440,031 (GRCm39)		probably benign	Het
Gask1a	A	T	9: 121,794,467 (GRCm39)	Q207L	probably damaging	Het
Golga3	G	A	5: 110,356,450 (GRCm39)		probably null	Het
Hint3	G	T	10: 30,486,380 (GRCm39)	A133E	probably damaging	Het
Hspe1	T	C	1: 55,128,376 (GRCm39)		probably null	Het
Insr	A	T	8: 3,308,418 (GRCm39)	V206E	probably benign	Het
Insrr	T	C	3: 87,722,551 (GRCm39)	S1258P	probably damaging	Het
Itga11	C	T	9: 62,659,538 (GRCm39)	T430M	probably damaging	Het
Jmjd1c	G	A	10: 67,056,388 (GRCm39)	V890I	possibly damaging	Het
Kdm4b	T	A	17: 56,703,592 (GRCm39)	S717R	probably damaging	Het
Large1	A	T	8: 73,843,092 (GRCm39)	C144S	probably damaging	Het
Lrat	G	T	3: 82,810,755 (GRCm39)	Q89K	probably benign	Het
Lrrc66	A	T	5: 73,765,899 (GRCm39)	F381L	possibly damaging	Het
Myo15a	A	G	11: 60,396,832 (GRCm39)	T2634A	probably damaging	Het
Nup205	T	G	6: 35,201,470 (GRCm39)	I1182M	possibly damaging	Het
Or2a51	T	A	6: 43,178,837 (GRCm39)	D86E	probably benign	Het
Or5m10	A	T	2: 85,717,965 (GRCm39)	I274F	probably benign	Het
Or6c66	T	A	10: 129,461,603 (GRCm39)	E109V	probably damaging	Het
Plekha6	T	A	1: 133,200,198 (GRCm39)	V259D	possibly damaging	Het
Prdm9	C	A	17: 15,765,257 (GRCm39)	A508S	possibly damaging	Het
Prickle2	A	G	6: 92,353,286 (GRCm39)	F783L	probably benign	Het
Ptprc	T	C	1: 138,054,047 (GRCm39)	S31G	probably benign	Het
Rbak	A	T	5: 143,159,226 (GRCm39)	I609N	probably damaging	Het
Rimklb	A	T	6: 122,436,176 (GRCm39)	L134*	probably null	Het
Ripor2	A	G	13: 24,877,749 (GRCm39)	I250V	probably benign	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Spaca6	C	T	17: 18,056,358 (GRCm39)	L118F	probably benign	Het
Tmem167	G	A	13: 90,246,533 (GRCm39)	C19Y	probably benign	Het
Togaram1	C	T	12: 65,067,160 (GRCm39)	T1684I	possibly damaging	Het
Trappc8	T	C	18: 21,007,729 (GRCm39)	T129A	probably benign	Het
Ubash3a	A	G	17: 31,447,184 (GRCm39)	S347G	probably benign	Het
Unc80	T	C	1: 66,542,752 (GRCm39)	S289P	probably benign	Het
Vmn2r11	A	G	5: 109,202,816 (GRCm39)	I87T	probably damaging	Het
Vmn2r54	A	T	7: 12,363,751 (GRCm39)	F381I	probably damaging	Het
Wdsub1	A	G	2: 59,683,224 (GRCm39)	L450P	probably damaging	Het
Xylt2	A	G	11: 94,558,408 (GRCm39)		probably null	Het
Zfp60	T	C	7: 27,448,451 (GRCm39)	I373T	probably benign	Het
Zfp738	A	G	13: 67,818,420 (GRCm39)	S524P	probably damaging	Het

Other mutations in Zfp429

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Zfp429	APN	13	67,539,132 (GRCm39)	missense	probably damaging	0.96
IGL01913:Zfp429	APN	13	67,544,793 (GRCm39)	missense	probably damaging	1.00
IGL02343:Zfp429	APN	13	67,538,844 (GRCm39)	missense	probably damaging	0.98
IGL02679:Zfp429	APN	13	67,547,855 (GRCm39)	intron	probably benign
IGL03396:Zfp429	APN	13	67,544,159 (GRCm39)	splice site	probably benign
FR4342:Zfp429	UTSW	13	67,544,769 (GRCm39)	missense	probably benign	0.02
R0012:Zfp429	UTSW	13	67,538,796 (GRCm39)	missense	probably benign	0.01
R1232:Zfp429	UTSW	13	67,538,751 (GRCm39)	missense	possibly damaging	0.47
R1330:Zfp429	UTSW	13	67,544,262 (GRCm39)	splice site	probably null
R1653:Zfp429	UTSW	13	67,538,043 (GRCm39)	missense	possibly damaging	0.87
R1761:Zfp429	UTSW	13	67,544,195 (GRCm39)	missense	probably benign	0.28
R1813:Zfp429	UTSW	13	67,538,505 (GRCm39)	missense	possibly damaging	0.55
R2356:Zfp429	UTSW	13	67,538,746 (GRCm39)	missense	probably benign
R4280:Zfp429	UTSW	13	67,538,914 (GRCm39)	missense	probably damaging	1.00
R4283:Zfp429	UTSW	13	67,538,914 (GRCm39)	missense	probably damaging	1.00
R4464:Zfp429	UTSW	13	67,538,617 (GRCm39)	missense	probably benign	0.13
R4789:Zfp429	UTSW	13	67,538,523 (GRCm39)	missense	probably benign	0.06
R5187:Zfp429	UTSW	13	67,538,959 (GRCm39)	missense	probably damaging	0.99
R5250:Zfp429	UTSW	13	67,538,638 (GRCm39)	missense	probably benign	0.00
R6688:Zfp429	UTSW	13	67,544,249 (GRCm39)	missense	probably damaging	0.98
R6772:Zfp429	UTSW	13	67,538,317 (GRCm39)	missense	probably damaging	1.00
R6989:Zfp429	UTSW	13	67,538,080 (GRCm39)	missense	probably benign	0.00
R7101:Zfp429	UTSW	13	67,538,931 (GRCm39)	missense	possibly damaging	0.88
R7593:Zfp429	UTSW	13	67,538,410 (GRCm39)	missense	probably damaging	1.00
R7792:Zfp429	UTSW	13	67,538,558 (GRCm39)	nonsense	probably null
R8500:Zfp429	UTSW	13	67,538,828 (GRCm39)	nonsense	probably null
R8721:Zfp429	UTSW	13	67,538,331 (GRCm39)	missense	probably damaging	0.98
R8891:Zfp429	UTSW	13	67,538,830 (GRCm39)	missense	probably damaging	1.00
R9364:Zfp429	UTSW	13	67,538,531 (GRCm39)	missense	probably benign	0.12
R9554:Zfp429	UTSW	13	67,538,531 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GCCACAGTCTTCACACTTGT -3'
(R):5'- TGGCTCAACAGGAATAACACC -3'

Sequencing Primer
(F):5'- GGAATAGCATTTGCCACAGTC -3'
(R):5'- CCCTACAAGTGTGGAGAATTTGG -3'

Posted On

2019-05-13