Mutagenetix > Incidental Mutation

Incidental Mutation 'R7226:Zfp976'

562157

Institutional Source

Beutler Lab

Gene Symbol

Zfp976

Ensembl Gene

ENSMUSG00000074158

Gene Name

zinc finger protein 976

Synonyms

9830147E19Rik

MMRRC Submission

045298-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R7226 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

42258950-42292012 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 42262684 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 385 (C385*)

Ref Sequence

ENSEMBL: ENSMUSP00000096105 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098503] [ENSMUST00000187616]

AlphaFold

E9Q981

Predicted Effect

probably null
Transcript: ENSMUST00000098503
AA Change: C385*

SMART Domains

Protein: ENSMUSP00000096105
Gene: ENSMUSG00000074158
AA Change: C385*

Domain	Start	End	E-Value	Type
KRAB	4	66	1.73e-18	SMART
ZnF_C2H2	131	153	7.78e-3	SMART
ZnF_C2H2	159	181	1.67e-2	SMART
ZnF_C2H2	187	209	5.9e-3	SMART
ZnF_C2H2	215	237	1.38e-3	SMART
ZnF_C2H2	243	265	8.94e-3	SMART
ZnF_C2H2	271	293	2.24e-3	SMART
ZnF_C2H2	299	321	1.03e-2	SMART
ZnF_C2H2	327	349	1.58e-3	SMART
ZnF_C2H2	355	377	7.9e-4	SMART
ZnF_C2H2	383	405	5.9e-3	SMART
ZnF_C2H2	411	433	2.57e-3	SMART
ZnF_C2H2	439	461	3.16e-3	SMART
ZnF_C2H2	467	489	1.26e-2	SMART
ZnF_C2H2	495	517	8.34e-3	SMART
ZnF_C2H2	523	545	3.63e-3	SMART
ZnF_C2H2	551	573	4.79e-3	SMART
ZnF_C2H2	579	601	9.73e-4	SMART
ZnF_C2H2	607	629	3.63e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000187616
AA Change: C384*

SMART Domains

Protein: ENSMUSP00000141023
Gene: ENSMUSG00000074158
AA Change: C384*

Domain	Start	End	E-Value	Type
KRAB	3	65	7.4e-21	SMART
ZnF_C2H2	130	152	3.2e-5	SMART
ZnF_C2H2	158	180	7.1e-5	SMART
ZnF_C2H2	186	208	2.4e-5	SMART
ZnF_C2H2	214	236	5.7e-6	SMART
ZnF_C2H2	242	264	3.8e-5	SMART
ZnF_C2H2	270	292	9.7e-6	SMART
ZnF_C2H2	298	320	4.2e-5	SMART
ZnF_C2H2	326	348	6.6e-6	SMART
ZnF_C2H2	354	376	3.3e-6	SMART
ZnF_C2H2	382	404	2.5e-5	SMART
ZnF_C2H2	410	432	1e-5	SMART
ZnF_C2H2	438	460	1.3e-5	SMART
ZnF_C2H2	466	488	5.2e-5	SMART
ZnF_C2H2	494	516	3.6e-5	SMART
ZnF_C2H2	522	544	1.6e-5	SMART
ZnF_C2H2	550	572	2e-5	SMART
ZnF_C2H2	578	600	4e-6	SMART
ZnF_C2H2	606	628	1.5e-5	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700025B11Rik	C	T	15: 77,443,189 (GRCm39)	V17I	unknown	Het
Acad8	T	A	9: 26,889,726 (GRCm39)	K323*	probably null	Het
Adam18	A	T	8: 25,137,824 (GRCm39)	C339S	probably damaging	Het
Anxa7	A	G	14: 20,510,263 (GRCm39)	F396L	probably damaging	Het
Ap2m1	T	A	16: 20,358,201 (GRCm39)	V73D	probably damaging	Het
Asic2	A	T	11: 80,862,340 (GRCm39)	I270N	probably damaging	Het
Atrn	T	G	2: 130,828,664 (GRCm39)	C1070G	probably damaging	Het
Bag6	A	G	17: 35,361,921 (GRCm39)	N517S	unknown	Het
Bhmt1b	A	G	18: 87,775,590 (GRCm39)	D371G	possibly damaging	Het
Bltp3b	A	T	10: 89,644,503 (GRCm39)	Q1181L	probably benign	Het
Cavin1	A	C	11: 100,861,284 (GRCm39)	D3E	probably benign	Het
Cc2d2b	T	C	19: 40,779,751 (GRCm39)	L571P	unknown	Het
Ccbe1	A	T	18: 66,216,199 (GRCm39)	C175S	probably damaging	Het
Cd19	A	G	7: 126,013,995 (GRCm39)	L5P	unknown	Het
Cd46	A	G	1: 194,724,314 (GRCm39)	S362P	possibly damaging	Het
Celf5	A	G	10: 81,303,863 (GRCm39)	S198P	probably damaging	Het
Cep170b	A	G	12: 112,704,359 (GRCm39)	R706G	possibly damaging	Het
Chd3	G	T	11: 69,260,037 (GRCm39)	R61S	unknown	Het
Cpsf6	A	C	10: 117,197,727 (GRCm39)	W253G	unknown	Het
Cyp27a1	G	A	1: 74,776,507 (GRCm39)	G481D	probably damaging	Het
Ddr1	C	T	17: 36,002,039 (GRCm39)	D218N	possibly damaging	Het
Dhx34	A	T	7: 15,932,801 (GRCm39)	V1046D	probably damaging	Het
Dhx9	A	T	1: 153,341,423 (GRCm39)	D608E	probably benign	Het
Dnajc6	C	A	4: 101,496,569 (GRCm39)	A912E	probably damaging	Het
Ehmt1	T	C	2: 24,694,794 (GRCm39)	D1051G	probably damaging	Het
Fam217b	C	A	2: 178,062,996 (GRCm39)	A320E	probably benign	Het
Farsa	T	A	8: 85,590,689 (GRCm39)	I169N	probably benign	Het
Fbn2	A	G	18: 58,170,142 (GRCm39)	C2210R	probably damaging	Het
Fbxo3	T	A	2: 103,880,642 (GRCm39)	S251T	probably benign	Het
Fer1l6	C	T	15: 58,462,384 (GRCm39)	T813I	probably benign	Het
Ftl1-ps1	A	T	13: 74,555,114 (GRCm39)	E131V	probably damaging	Het
Gins3	T	A	8: 96,364,499 (GRCm39)	I83N	probably damaging	Het
Gja3	T	C	14: 57,273,350 (GRCm39)	T341A	probably benign	Het
Gja5	A	G	3: 96,958,174 (GRCm39)	Y77C	probably damaging	Het
Gm7247	A	G	14: 51,602,808 (GRCm39)	K48R	probably damaging	Het
Gsx2	C	A	5: 75,236,621 (GRCm39)	S67*	probably null	Het
H2-Q5	T	A	17: 35,616,089 (GRCm39)	L217Q		Het
Impg2	T	A	16: 56,087,467 (GRCm39)	C1095*	probably null	Het
Itga7	G	T	10: 128,776,801 (GRCm39)	W222L	probably damaging	Het
Kdm2b	T	A	5: 123,059,512 (GRCm39)	D530V	possibly damaging	Het
Klhdc8a	A	T	1: 132,230,344 (GRCm39)	D153V	probably damaging	Het
Lin28a	C	T	4: 133,733,619 (GRCm39)	G143S	probably damaging	Het
Memo1	A	G	17: 74,509,338 (GRCm39)	L227S	probably damaging	Het
Mettl27	T	A	5: 134,964,657 (GRCm39)	V138E	probably damaging	Het
Mov10	G	T	3: 104,708,328 (GRCm39)	L474I	probably damaging	Het
Nat10	T	C	2: 103,557,098 (GRCm39)	N852S	probably benign	Het
Nfkbie	T	C	17: 45,870,153 (GRCm39)	V166A	possibly damaging	Het
Nktr	T	C	9: 121,575,599 (GRCm39)	M369T	probably damaging	Het
Nlrp9a	A	T	7: 26,258,149 (GRCm39)	N589I	probably benign	Het
Nrn1	G	A	13: 36,914,577 (GRCm39)	R8C	probably benign	Het
Nrsn1	A	G	13: 25,437,451 (GRCm39)	I159T	probably damaging	Het
Or1e29	A	G	11: 73,667,503 (GRCm39)	S217P	possibly damaging	Het
Or2h15	C	T	17: 38,441,324 (GRCm39)	G253D	probably benign	Het
Or5p61	A	T	7: 107,758,164 (GRCm39)	D305E	probably benign	Het
Or6c88	G	A	10: 129,406,584 (GRCm39)	G20D	probably benign	Het
Osbp	T	A	19: 11,956,031 (GRCm39)	D385E	probably benign	Het
Pan3	T	A	5: 147,463,802 (GRCm39)	N547K	probably damaging	Het
Pip4p2	T	A	4: 14,892,464 (GRCm39)	D109E	probably damaging	Het
Pum1	C	A	4: 130,499,292 (GRCm39)	T1036K	probably damaging	Het
Rad54l2	C	A	9: 106,590,671 (GRCm39)	R485L	probably damaging	Het
Rps26	A	T	10: 128,461,087 (GRCm39)	F101I	unknown	Het
Sdsl	T	C	5: 120,598,702 (GRCm39)	N138S	probably benign	Het
Slc28a2b	C	A	2: 122,353,013 (GRCm39)	R453S	probably benign	Het
Slc39a6	A	T	18: 24,717,084 (GRCm39)	H649Q	probably damaging	Het
Snip1	T	A	4: 124,965,273 (GRCm39)	F226Y	probably benign	Het
Srrd	T	C	5: 112,485,322 (GRCm39)	T334A	unknown	Het
St14	C	T	9: 31,011,448 (GRCm39)	D448N	possibly damaging	Het
Tmem270	T	A	5: 134,930,538 (GRCm39)	Q241L	probably benign	Het
Tubb4b	T	C	2: 25,114,180 (GRCm39)	D41G	probably benign	Het
Usf3	T	C	16: 44,040,368 (GRCm39)	M1616T	possibly damaging	Het
Vmn2r95	A	T	17: 18,672,245 (GRCm39)	I733F	possibly damaging	Het

Other mutations in Zfp976

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp976	APN	7	42,263,109 (GRCm39)	missense	unknown
IGL01102:Zfp976	APN	7	42,263,333 (GRCm39)	nonsense	probably null
IGL01111:Zfp976	APN	7	42,265,711 (GRCm39)	missense	probably damaging	0.99
IGL01628:Zfp976	APN	7	42,261,935 (GRCm39)	missense	unknown
IGL02008:Zfp976	APN	7	42,263,656 (GRCm39)	splice site	probably benign
IGL02548:Zfp976	APN	7	42,261,953 (GRCm39)	missense	unknown
R0190:Zfp976	UTSW	7	42,291,948 (GRCm39)	start gained	probably benign
R0685:Zfp976	UTSW	7	42,263,141 (GRCm39)	missense	probably damaging	0.98
R1310:Zfp976	UTSW	7	42,262,610 (GRCm39)	missense	probably damaging	1.00
R1353:Zfp976	UTSW	7	42,265,442 (GRCm39)	missense	probably damaging	0.99
R1447:Zfp976	UTSW	7	42,262,023 (GRCm39)	missense	possibly damaging	0.79
R1569:Zfp976	UTSW	7	42,262,806 (GRCm39)	missense	probably damaging	1.00
R1702:Zfp976	UTSW	7	42,265,424 (GRCm39)	missense	possibly damaging	0.86
R1829:Zfp976	UTSW	7	42,265,735 (GRCm39)	missense	probably damaging	1.00
R1939:Zfp976	UTSW	7	42,263,105 (GRCm39)	missense	unknown
R1978:Zfp976	UTSW	7	42,263,265 (GRCm39)	missense	probably damaging	1.00
R1981:Zfp976	UTSW	7	42,263,046 (GRCm39)	missense	probably damaging	0.99
R2160:Zfp976	UTSW	7	42,263,354 (GRCm39)	missense	probably benign
R2192:Zfp976	UTSW	7	42,262,695 (GRCm39)	missense	probably damaging	1.00
R3121:Zfp976	UTSW	7	42,262,938 (GRCm39)	missense	probably damaging	1.00
R4210:Zfp976	UTSW	7	42,265,749 (GRCm39)	missense	probably damaging	0.99
R4724:Zfp976	UTSW	7	42,262,457 (GRCm39)	missense	possibly damaging	0.91
R4943:Zfp976	UTSW	7	42,261,846 (GRCm39)	unclassified	probably benign
R5047:Zfp976	UTSW	7	42,262,843 (GRCm39)	nonsense	probably null
R5071:Zfp976	UTSW	7	42,262,354 (GRCm39)	nonsense	probably null
R5125:Zfp976	UTSW	7	42,261,925 (GRCm39)	splice site	probably null
R5178:Zfp976	UTSW	7	42,261,925 (GRCm39)	splice site	probably null
R5305:Zfp976	UTSW	7	42,262,902 (GRCm39)	missense	probably benign	0.00
R5777:Zfp976	UTSW	7	42,263,504 (GRCm39)	missense	probably benign	0.00
R6153:Zfp976	UTSW	7	42,263,610 (GRCm39)	missense	probably damaging	0.99
R6694:Zfp976	UTSW	7	42,263,610 (GRCm39)	missense	probably damaging	0.99
R7479:Zfp976	UTSW	7	42,262,603 (GRCm39)	missense	probably benign	0.01
R7561:Zfp976	UTSW	7	42,265,701 (GRCm39)	missense	probably damaging	1.00
R8178:Zfp976	UTSW	7	42,262,959 (GRCm39)	missense	probably benign	0.03
R8261:Zfp976	UTSW	7	42,262,125 (GRCm39)	missense	unknown
R8715:Zfp976	UTSW	7	42,262,869 (GRCm39)	missense	possibly damaging	0.89
R8921:Zfp976	UTSW	7	42,262,575 (GRCm39)	missense	possibly damaging	0.57
R9168:Zfp976	UTSW	7	42,263,011 (GRCm39)	nonsense	probably null
R9575:Zfp976	UTSW	7	42,262,041 (GRCm39)	missense	unknown
Z1088:Zfp976	UTSW	7	42,262,184 (GRCm39)	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- CTGGGATGTAAGAAGGCTTTATCA -3'
(R):5'- TTCACACCAGAGTAATCTCCGAATAC -3'

Sequencing Primer
(F):5'- GGATGTAAGAAGGCTTTATCACATTC -3'
(R):5'- CTGTGTGATAAAGCCTATTCACACC -3'

Posted On

2019-06-26