Mutagenetix > Incidental Mutation

Incidental Mutation 'R0894:Fryl'

83649

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

9030227G01Rik, 2310004H21Rik, 2510002A14Rik

MMRRC Submission

039057-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.740) question?

Stock #

R0894 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73177534-73414042 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 73198675 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094700

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000101127

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146953

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156661

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000202292

Predicted Effect

probably benign
Transcript: ENSMUST00000202381

Predicted Effect

probably benign
Transcript: ENSMUST00000202697

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 97.7%
20x: 95.8%

Validation Efficiency

97% (102/105)

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	G	13: 77,471,726 (GRCm39)	S1044G	probably benign	Het
Aanat	A	G	11: 116,487,730 (GRCm39)	H143R	probably benign	Het
Abca8a	A	G	11: 109,941,792 (GRCm39)	I1159T	probably benign	Het
Abcb1a	G	T	5: 8,724,856 (GRCm39)		probably benign	Het
Abcc1	T	C	16: 14,283,001 (GRCm39)	V1159A	possibly damaging	Het
Akr1e1	T	A	13: 4,645,071 (GRCm39)	Q204L	probably damaging	Het
Alk	T	C	17: 72,202,930 (GRCm39)	Y1135C	probably damaging	Het
Atad2b	C	T	12: 5,015,915 (GRCm39)	T547I	probably damaging	Het
Brd10	T	C	19: 29,697,974 (GRCm39)		probably benign	Het
C030005K15Rik	A	T	10: 97,561,648 (GRCm39)	S28T	unknown	Het
Cdk5rap3	A	G	11: 96,799,654 (GRCm39)	L387P	probably damaging	Het
Cfap95	T	C	19: 23,630,062 (GRCm39)	E10G	unknown	Het
Cfap96	A	G	8: 46,409,497 (GRCm39)	F274S	probably damaging	Het
Clec4f	T	A	6: 83,629,979 (GRCm39)	N193I	probably damaging	Het
Col4a4	A	T	1: 82,507,377 (GRCm39)		probably null	Het
Cplx4	T	G	18: 66,090,116 (GRCm39)	D101A	possibly damaging	Het
Cpne8	A	T	15: 90,533,474 (GRCm39)	D50E	probably damaging	Het
Csmd3	G	T	15: 47,721,316 (GRCm39)	D1542E	possibly damaging	Het
Ctdp1	A	G	18: 80,512,736 (GRCm39)	V9A	probably benign	Het
Ctnnd2	A	T	15: 30,332,301 (GRCm39)		probably benign	Het
Cyp7b1	C	T	3: 18,151,674 (GRCm39)	A180T	probably benign	Het
Dcun1d5	C	T	9: 7,203,379 (GRCm39)		probably benign	Het
Dgat1	G	T	15: 76,387,199 (GRCm39)	L363I	possibly damaging	Het
Dipk2b	T	A	X: 18,289,791 (GRCm39)	I224F	possibly damaging	Het
Dlg1	T	A	16: 31,561,965 (GRCm39)	H120Q	probably benign	Het
Dnah6	T	C	6: 73,101,740 (GRCm39)	N1928S	probably benign	Het
Dnai4	T	C	4: 102,906,583 (GRCm39)		probably benign	Het
Dync2h1	A	T	9: 7,041,734 (GRCm39)		probably benign	Het
Ednra	T	G	8: 78,446,649 (GRCm39)		probably benign	Het
Efcab6	A	T	15: 83,802,493 (GRCm39)	C845S	probably benign	Het
Egln1	A	T	8: 125,642,435 (GRCm39)	C303S	probably damaging	Het
Eomes	T	C	9: 118,311,368 (GRCm39)		probably null	Het
Epha1	A	G	6: 42,340,756 (GRCm39)	V568A	probably benign	Het
Ercc6	T	A	14: 32,238,985 (GRCm39)	N24K	probably benign	Het
Esco2	T	C	14: 66,064,726 (GRCm39)	Q338R	probably benign	Het
Fbxo46	T	C	7: 18,869,654 (GRCm39)	V91A	probably damaging	Het
Gab3	A	C	X: 74,077,024 (GRCm39)	D43E	probably damaging	Het
Gltpd2	T	A	11: 70,410,535 (GRCm39)		probably benign	Het
Gm17333	G	T	16: 77,649,711 (GRCm39)		noncoding transcript	Het
Gm7353	T	C	7: 3,160,570 (GRCm39)		noncoding transcript	Het
Grik4	T	C	9: 42,599,405 (GRCm39)		probably benign	Het
Gtpbp2	G	T	17: 46,476,895 (GRCm39)	A358S	possibly damaging	Het
Hyls1	C	T	9: 35,472,528 (GRCm39)	C296Y	probably damaging	Het
Igf2r	C	T	17: 12,910,988 (GRCm39)	M1943I	probably benign	Het
Iqca1l	T	C	5: 24,755,731 (GRCm39)		probably null	Het
Ireb2	T	A	9: 54,803,861 (GRCm39)	N517K	probably damaging	Het
Itga10	A	G	3: 96,560,976 (GRCm39)	S614G	possibly damaging	Het
Kdm2b	A	G	5: 123,122,523 (GRCm39)		probably null	Het
Kif17	T	C	4: 138,025,542 (GRCm39)	M948T	possibly damaging	Het
Klhl33	A	T	14: 51,129,583 (GRCm39)	N347K	probably damaging	Het
Llph	T	A	10: 120,064,086 (GRCm39)	C67*	probably null	Het
Lrrn3	T	C	12: 41,504,033 (GRCm39)	T95A	probably damaging	Het
Map3k12	C	A	15: 102,410,613 (GRCm39)	A455S	probably damaging	Het
Mex3d	T	C	10: 80,217,376 (GRCm39)	T149A	probably benign	Het
Myo7b	A	G	18: 32,133,123 (GRCm39)	W409R	probably damaging	Het
Nbea	A	G	3: 55,916,761 (GRCm39)	M833T	possibly damaging	Het
Ncapg	A	G	5: 45,837,236 (GRCm39)	T436A	probably null	Het
Nkx1-2	C	A	7: 132,201,042 (GRCm39)	D72Y	probably null	Het
Or11g27	T	C	14: 50,771,159 (GRCm39)	S97P	possibly damaging	Het
Or2d3b	T	A	7: 106,514,317 (GRCm39)	I304K	probably benign	Het
Or6c217	T	A	10: 129,737,751 (GRCm39)	N276I	probably damaging	Het
Pcdh15	A	G	10: 74,460,087 (GRCm39)	Y1308C	probably damaging	Het
Pcnx2	A	G	8: 126,613,665 (GRCm39)		probably benign	Het
Pcsk1	G	A	13: 75,246,096 (GRCm39)	G158D	probably damaging	Het
Phkb	A	T	8: 86,744,070 (GRCm39)	D573V	probably damaging	Het
Pik3r4	A	G	9: 105,544,970 (GRCm39)	K150E	possibly damaging	Het
Ppp2r5e	C	G	12: 75,516,341 (GRCm39)	A239P	probably damaging	Het
Ppp4r4	C	T	12: 103,566,754 (GRCm39)	A67V	probably damaging	Het
Prex2	A	G	1: 11,252,122 (GRCm39)	T1056A	probably benign	Het
Prkca	A	T	11: 107,903,518 (GRCm39)	Y285N	possibly damaging	Het
Psd	T	C	19: 46,301,880 (GRCm39)	E903G	probably damaging	Het
Psg19	T	C	7: 18,527,987 (GRCm39)	E252G	probably benign	Het
Psg20	T	A	7: 18,414,969 (GRCm39)	K306*	probably null	Het
Pygl	T	G	12: 70,241,148 (GRCm39)		probably benign	Het
Rasgrf2	A	G	13: 92,130,890 (GRCm39)	S724P	probably damaging	Het
Reck	C	A	4: 43,922,967 (GRCm39)	A414D	probably damaging	Het
Scn10a	C	A	9: 119,459,213 (GRCm39)	V1150L	probably damaging	Het
Shc2	A	T	10: 79,465,751 (GRCm39)	I187N	probably damaging	Het
Sipa1l3	T	A	7: 29,086,716 (GRCm39)	K625*	probably null	Het
Slc44a4	T	C	17: 35,147,466 (GRCm39)	L583P	possibly damaging	Het
Slc5a11	G	C	7: 122,857,643 (GRCm39)	R244P	possibly damaging	Het
Slfn8	T	A	11: 82,894,407 (GRCm39)	Q744L	probably benign	Het
Snx2	G	T	18: 53,309,488 (GRCm39)	V13L	probably benign	Het
Spmip5	A	T	19: 58,776,015 (GRCm39)	L138Q	probably damaging	Het
Spsb1	C	T	4: 149,990,872 (GRCm39)		probably null	Het
Stfa2l1	A	T	16: 35,977,228 (GRCm39)	I8L	probably benign	Het
Svil	G	T	18: 5,097,494 (GRCm39)	R1659L	probably damaging	Het
Tbccd1	A	T	16: 22,640,995 (GRCm39)	L461M	probably benign	Het
Tmem9	A	T	1: 135,961,926 (GRCm39)	T174S	possibly damaging	Het
Tnks2	T	C	19: 36,867,450 (GRCm39)		probably null	Het
Tnrc18	C	A	5: 142,800,869 (GRCm39)	V30L	probably benign	Het
Tomm7	A	G	5: 24,049,025 (GRCm39)	F16S	probably damaging	Het
Ttf2	A	G	3: 100,876,865 (GRCm39)		probably benign	Het
Ubr7	C	A	12: 102,735,450 (GRCm39)	T303N	probably damaging	Het
Ushbp1	A	T	8: 71,842,868 (GRCm39)		probably null	Het
Vmn1r177	C	A	7: 23,565,475 (GRCm39)	V134F	probably benign	Het
Vmn2r12	A	G	5: 109,235,716 (GRCm39)		probably null	Het
Vmn2r53	T	G	7: 12,335,141 (GRCm39)	H173P	probably benign	Het
Yars1	A	T	4: 129,090,948 (GRCm39)	M119L	probably damaging	Het
Zcrb1	A	T	15: 93,295,038 (GRCm39)		probably benign	Het
Zfp267	T	A	3: 36,218,935 (GRCm39)	Y319*	probably null	Het
Zfp319	C	A	8: 96,056,250 (GRCm39)		probably benign	Het
Zfp783	C	G	6: 47,920,320 (GRCm39)		noncoding transcript	Het
Zfyve26	A	G	12: 79,320,372 (GRCm39)	I1024T	possibly damaging	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,305,451 (GRCm39)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,244,305 (GRCm39)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,211,940 (GRCm39)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,179,844 (GRCm39)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,279,707 (GRCm39)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,190,134 (GRCm39)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,282,112 (GRCm39)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,233,302 (GRCm39)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,267,519 (GRCm39)	splice site	probably benign
IGL02431:Fryl	APN	5	73,255,651 (GRCm39)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,222,636 (GRCm39)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,255,736 (GRCm39)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,227,220 (GRCm39)	intron	probably benign
IGL02642:Fryl	APN	5	73,252,809 (GRCm39)	missense	probably benign
IGL02657:Fryl	APN	5	73,212,203 (GRCm39)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,250,506 (GRCm39)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,216,726 (GRCm39)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,258,798 (GRCm39)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03183:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03275:Fryl	APN	5	73,305,376 (GRCm39)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,293,659 (GRCm39)	splice site	probably benign
IGL03341:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03343:Fryl	APN	5	73,234,038 (GRCm39)	missense	probably benign
IGL03350:Fryl	APN	5	73,290,649 (GRCm39)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,211,402 (GRCm39)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,267,624 (GRCm39)	splice site	probably benign
IGL03375:Fryl	APN	5	73,245,792 (GRCm39)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
Besotted	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,179,621 (GRCm39)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,198,947 (GRCm39)	splice site	probably benign
R0312:Fryl	UTSW	5	73,230,231 (GRCm39)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,255,757 (GRCm39)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,244,315 (GRCm39)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,254,760 (GRCm39)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,221,840 (GRCm39)	splice site	probably benign
R0567:Fryl	UTSW	5	73,222,734 (GRCm39)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,282,077 (GRCm39)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,226,074 (GRCm39)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,222,702 (GRCm39)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,240,715 (GRCm39)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,228,469 (GRCm39)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,246,424 (GRCm39)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,246,539 (GRCm39)	missense	probably damaging	0.97
R1076:Fryl	UTSW	5	73,282,016 (GRCm39)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,267,614 (GRCm39)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,222,268 (GRCm39)	splice site	probably benign
R1394:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,232,094 (GRCm39)	nonsense	probably null
R1664:Fryl	UTSW	5	73,216,778 (GRCm39)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,190,204 (GRCm39)	splice site	probably benign
R1937:Fryl	UTSW	5	73,290,710 (GRCm39)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,265,836 (GRCm39)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,179,465 (GRCm39)	nonsense	probably null
R2036:Fryl	UTSW	5	73,265,305 (GRCm39)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,179,887 (GRCm39)	missense	probably benign
R2088:Fryl	UTSW	5	73,222,804 (GRCm39)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,279,642 (GRCm39)	missense	probably benign
R2106:Fryl	UTSW	5	73,255,674 (GRCm39)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,222,318 (GRCm39)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,265,890 (GRCm39)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,230,187 (GRCm39)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,198,707 (GRCm39)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,207,799 (GRCm39)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,240,193 (GRCm39)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,265,417 (GRCm39)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3787:Fryl	UTSW	5	73,258,819 (GRCm39)	missense	probably benign
R3837:Fryl	UTSW	5	73,228,608 (GRCm39)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,269,766 (GRCm39)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,243,903 (GRCm39)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,245,740 (GRCm39)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,238,396 (GRCm39)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,248,022 (GRCm39)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,257,636 (GRCm39)	missense	probably benign
R4700:Fryl	UTSW	5	73,222,881 (GRCm39)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,238,315 (GRCm39)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,198,705 (GRCm39)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,226,125 (GRCm39)	frame shift	probably null
R4948:Fryl	UTSW	5	73,246,473 (GRCm39)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,192,401 (GRCm39)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,233,236 (GRCm39)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,215,098 (GRCm39)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,232,110 (GRCm39)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,248,061 (GRCm39)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,259,016 (GRCm39)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,243,943 (GRCm39)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,222,479 (GRCm39)	nonsense	probably null
R5275:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,270,134 (GRCm39)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,262,117 (GRCm39)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,231,247 (GRCm39)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,257,808 (GRCm39)	missense	probably benign
R5778:Fryl	UTSW	5	73,230,121 (GRCm39)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,248,098 (GRCm39)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,248,060 (GRCm39)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,254,715 (GRCm39)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,240,638 (GRCm39)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,257,340 (GRCm39)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,275,894 (GRCm39)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,270,131 (GRCm39)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,222,824 (GRCm39)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,349,102 (GRCm39)	intron	probably benign
R6310:Fryl	UTSW	5	73,349,104 (GRCm39)	intron	probably benign
R6429:Fryl	UTSW	5	73,248,094 (GRCm39)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,216,859 (GRCm39)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,290,655 (GRCm39)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,289,824 (GRCm39)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,212,124 (GRCm39)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,179,575 (GRCm39)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,222,437 (GRCm39)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,222,560 (GRCm39)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,216,843 (GRCm39)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,222,375 (GRCm39)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,226,146 (GRCm39)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,179,485 (GRCm39)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,179,554 (GRCm39)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,231,272 (GRCm39)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,207,773 (GRCm39)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,212,951 (GRCm39)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,248,099 (GRCm39)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,231,251 (GRCm39)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,279,653 (GRCm39)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,230,255 (GRCm39)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,222,438 (GRCm39)	missense	probably benign
R7236:Fryl	UTSW	5	73,265,821 (GRCm39)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,204,839 (GRCm39)	splice site	probably null
R7425:Fryl	UTSW	5	73,262,091 (GRCm39)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,181,331 (GRCm39)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,254,904 (GRCm39)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,255,539 (GRCm39)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,180,019 (GRCm39)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,238,382 (GRCm39)	missense	probably benign
R7548:Fryl	UTSW	5	73,349,105 (GRCm39)	missense	unknown
R7565:Fryl	UTSW	5	73,191,063 (GRCm39)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,245,739 (GRCm39)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,179,843 (GRCm39)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,267,588 (GRCm39)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,240,727 (GRCm39)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,228,641 (GRCm39)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,211,875 (GRCm39)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,259,150 (GRCm39)	splice site	probably null
R8110:Fryl	UTSW	5	73,290,620 (GRCm39)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,228,527 (GRCm39)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,207,682 (GRCm39)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,257,843 (GRCm39)	splice site	probably null
R8263:Fryl	UTSW	5	73,238,348 (GRCm39)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8359:Fryl	UTSW	5	73,233,276 (GRCm39)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,293,663 (GRCm39)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,275,790 (GRCm39)	makesense	probably null
R8450:Fryl	UTSW	5	73,226,073 (GRCm39)	missense	probably benign
R8514:Fryl	UTSW	5	73,242,699 (GRCm39)	missense	probably benign
R8536:Fryl	UTSW	5	73,257,696 (GRCm39)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,247,997 (GRCm39)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,289,905 (GRCm39)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,226,185 (GRCm39)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,255,609 (GRCm39)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,182,118 (GRCm39)	missense
R9063:Fryl	UTSW	5	73,238,346 (GRCm39)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,265,920 (GRCm39)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,348,862 (GRCm39)	intron	probably benign
R9345:Fryl	UTSW	5	73,207,754 (GRCm39)	missense	probably benign
R9381:Fryl	UTSW	5	73,240,637 (GRCm39)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,349,152 (GRCm39)	missense	unknown
R9401:Fryl	UTSW	5	73,222,563 (GRCm39)	nonsense	probably null
R9497:Fryl	UTSW	5	73,215,134 (GRCm39)	missense
R9514:Fryl	UTSW	5	73,262,115 (GRCm39)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,179,498 (GRCm39)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,275,801 (GRCm39)	missense	probably benign
R9665:Fryl	UTSW	5	73,222,299 (GRCm39)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,216,879 (GRCm39)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,192,402 (GRCm39)	missense	probably benign
Z1088:Fryl	UTSW	5	73,248,081 (GRCm39)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,248,052 (GRCm39)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,230,180 (GRCm39)	missense	probably benign
Z1177:Fryl	UTSW	5	73,198,938 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- ACACTTGGCCGTTTGGTATTCTGC -3'
(R):5'- AGGAGGAACACGTTACAGCCTTTG -3'

Sequencing Primer
(F):5'- CGTTTGGTATTCTGCCTAAACAG -3'
(R):5'- CGAAGGGTCATACGTTATTCCAG -3'

Posted On

2013-11-08