Mutagenetix > Incidental Mutation

Incidental Mutation 'R1478:Tafa2'

165641

Institutional Source

Beutler Lab

Gene Symbol

Tafa2

Ensembl Gene

ENSMUSG00000044071

Gene Name

TAFA chemokine like family member 2

Synonyms

Sam2, Tafa2, Fam19a2, Tafa-2

MMRRC Submission

039531-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.214) question?

Stock #

R1478 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123099901-123577109 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 123429401 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 5 (M5K)

Ref Sequence

ENSEMBL: ENSMUSP00000050199 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050756]

AlphaFold

Q7TPG7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000050756
AA Change: M5K

PolyPhen 2 Score 0.856 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000050199
Gene: ENSMUSG00000044071
AA Change: M5K

Domain	Start	End	E-Value	Type
Pfam:TAFA	45	133	6.8e-51	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000218926

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219723

Meta Mutation Damage Score

0.8757

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.7%
20x: 90.6%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased anxiety-like and fear-related behaviors. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aacs	T	A	5: 125,580,287 (GRCm39)	I204N	possibly damaging	Het
Acaca	A	G	11: 84,263,453 (GRCm39)	T2025A	probably damaging	Het
B4galt3	C	T	1: 171,103,938 (GRCm39)	R170C	probably benign	Het
BC028528	T	C	3: 95,799,271 (GRCm39)		probably null	Het
Ccdc73	A	T	2: 104,737,955 (GRCm39)	Q17L	possibly damaging	Het
Ccdc73	A	G	2: 104,745,012 (GRCm39)	T50A	possibly damaging	Het
Ccdc83	T	C	7: 89,908,677 (GRCm39)	D19G	probably damaging	Het
Ccn6	G	A	10: 39,029,239 (GRCm39)	R230W	probably damaging	Het
Cct8l1	T	C	5: 25,722,767 (GRCm39)	V494A	probably benign	Het
Chd1	T	C	17: 15,959,769 (GRCm39)	F663L	probably damaging	Het
Chn2	A	T	6: 54,270,065 (GRCm39)	E319V	probably damaging	Het
Cnnm1	T	C	19: 43,460,295 (GRCm39)	V679A	probably damaging	Het
Cntn6	C	T	6: 104,753,389 (GRCm39)	T447I	probably benign	Het
Cyp3a11	G	T	5: 145,795,581 (GRCm39)	L457I	probably benign	Het
Dera	T	A	6: 137,807,193 (GRCm39)	S214T	possibly damaging	Het
Dmtf1	A	G	5: 9,171,404 (GRCm39)	V501A	possibly damaging	Het
Dnpep	A	G	1: 75,292,671 (GRCm39)	V114A	probably damaging	Het
Dscam	C	T	16: 96,592,110 (GRCm39)	V722M	probably benign	Het
Dsp	A	G	13: 38,365,114 (GRCm39)	N499S	probably damaging	Het
Ecd	A	T	14: 20,396,725 (GRCm39)	Y53*	probably null	Het
Esyt3	T	C	9: 99,200,119 (GRCm39)	T692A	probably benign	Het
Fat1	T	A	8: 45,478,659 (GRCm39)	N2545K	probably damaging	Het
Fndc3a	A	G	14: 72,795,072 (GRCm39)		probably null	Het
Fscn3	A	G	6: 28,430,567 (GRCm39)	M246V	probably benign	Het
Gde1	G	T	7: 118,291,007 (GRCm39)	T106K	probably benign	Het
Gjc2	A	G	11: 59,068,434 (GRCm39)	I16T	possibly damaging	Het
Hacd4	A	T	4: 88,341,260 (GRCm39)	M168K	probably damaging	Het
Hmx3	A	G	7: 131,145,826 (GRCm39)	E178G	probably damaging	Het
Htt	A	G	5: 34,961,171 (GRCm39)	Y266C	probably damaging	Het
Jkampl	A	T	6: 73,446,039 (GRCm39)	L170Q	probably damaging	Het
Kif27	G	T	13: 58,451,359 (GRCm39)	R990S	probably damaging	Het
Krtap22-2	A	T	16: 88,807,534 (GRCm39)	Y21*	probably null	Het
Lrrc41	T	A	4: 115,952,405 (GRCm39)	L661*	probably null	Het
Lrrc63	T	C	14: 75,363,424 (GRCm39)	T236A	probably benign	Het
Mmp13	T	C	9: 7,272,892 (GRCm39)	L84P	probably damaging	Het
Myh8	T	C	11: 67,183,551 (GRCm39)	I754T	probably benign	Het
Neb	A	C	2: 52,065,619 (GRCm39)	D5961E	probably benign	Het
Nlrx1	T	C	9: 44,175,374 (GRCm39)	H134R	probably benign	Het
Npepps	A	T	11: 97,117,673 (GRCm39)	M542K	probably benign	Het
Nup160	C	T	2: 90,509,743 (GRCm39)		probably benign	Het
Or3a1c	T	A	11: 74,045,963 (GRCm39)		probably null	Het
Or8g33	T	A	9: 39,337,888 (GRCm39)	T160S	possibly damaging	Het
Pikfyve	T	C	1: 65,302,136 (GRCm39)		probably null	Het
Ppp6r1	A	T	7: 4,643,377 (GRCm39)		probably null	Het
Prss33	A	G	17: 24,054,072 (GRCm39)	W45R	probably damaging	Het
Ptprf	A	T	4: 118,069,302 (GRCm39)	Y980*	probably null	Het
Qrich1	T	C	9: 108,436,531 (GRCm39)	V743A	probably benign	Het
Rev3l	G	A	10: 39,659,329 (GRCm39)		probably null	Het
Samd9l	A	T	6: 3,376,369 (GRCm39)	N297K	probably benign	Het
Sap130	A	G	18: 31,813,527 (GRCm39)	H528R	possibly damaging	Het
Slc9b2	T	C	3: 135,031,863 (GRCm39)	V241A	probably benign	Het
Snx14	A	T	9: 88,276,581 (GRCm39)	V577E	probably benign	Het
Srrm2	T	C	17: 24,034,876 (GRCm39)	S507P	probably benign	Het
Stat2	A	G	10: 128,117,969 (GRCm39)		probably null	Het
Susd5	T	C	9: 113,925,752 (GRCm39)	F545S	probably benign	Het
Tnnt2	A	G	1: 135,775,764 (GRCm39)	T107A	probably benign	Het
Trappc6b	T	A	12: 59,094,953 (GRCm39)	I41F	possibly damaging	Het
Ttn	T	A	2: 76,682,417 (GRCm39)		probably benign	Het
Vmn1r88	A	C	7: 12,911,878 (GRCm39)	D78A	probably damaging	Het
Xpc	C	T	6: 91,485,510 (GRCm39)	D122N	possibly damaging	Het
Xpo1	C	T	11: 23,241,623 (GRCm39)	A890V	probably damaging	Het
Zfp493	A	G	13: 67,934,680 (GRCm39)	H211R	probably damaging	Het
Zfp958	A	T	8: 4,679,190 (GRCm39)	H405L	probably damaging	Het

Other mutations in Tafa2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
dolorous	UTSW	10	123,540,297 (GRCm39)	missense	probably damaging	1.00
Lugubrious	UTSW	10	123,429,401 (GRCm39)	missense	possibly damaging	0.86
R0395:Tafa2	UTSW	10	123,429,497 (GRCm39)	missense	probably benign	0.05
R2869:Tafa2	UTSW	10	123,540,270 (GRCm39)	missense	possibly damaging	0.63
R2869:Tafa2	UTSW	10	123,540,270 (GRCm39)	missense	possibly damaging	0.63
R2870:Tafa2	UTSW	10	123,540,270 (GRCm39)	missense	possibly damaging	0.63
R2870:Tafa2	UTSW	10	123,540,270 (GRCm39)	missense	possibly damaging	0.63
R2873:Tafa2	UTSW	10	123,540,270 (GRCm39)	missense	possibly damaging	0.63
R6537:Tafa2	UTSW	10	123,429,401 (GRCm39)	missense	possibly damaging	0.86
R6589:Tafa2	UTSW	10	123,540,297 (GRCm39)	missense	probably damaging	1.00
R6972:Tafa2	UTSW	10	123,540,278 (GRCm39)	missense	probably benign	0.01
R9043:Tafa2	UTSW	10	123,540,294 (GRCm39)	missense	probably damaging	1.00
R9065:Tafa2	UTSW	10	123,429,421 (GRCm39)	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- TGAGTCATAGCAACATGCTTCATTTGGA -3'
(R):5'- AGAAAATTGCTAAGGGGAACCCATACC -3'

Sequencing Primer
(F):5'- aacaaccacacaatcaccaac -3'
(R):5'- GGGGAACCCATACCTAATAAAATTG -3'

Posted On

2014-03-28