Incidental Mutation 'R7275:Pde8b'
| ID |
565533 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Pde8b
|
| Ensembl Gene |
ENSMUSG00000021684 |
| Gene Name |
phosphodiesterase 8B |
| Synonyms |
B230331L10Rik, C030047E14Rik |
| MMRRC Submission |
045358-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.110)
|
| Stock # |
R7275 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
13 |
| Chromosomal Location |
95160962-95386844 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 95179442 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Asparagine to Tyrosine
at position 405
(N405Y)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000124409
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000022192]
[ENSMUST00000067082]
[ENSMUST00000159608]
[ENSMUST00000160957]
[ENSMUST00000162153]
[ENSMUST00000162292]
[ENSMUST00000162412]
[ENSMUST00000172104]
|
| AlphaFold |
E9Q4S1 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000022192
AA Change: N513Y
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000022192
Gene: ENSMUSG00000021684
AA Change: N513Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDE8
|
1 |
52 |
2.2e-39 |
PFAM |
|
low complexity region
|
75 |
98 |
N/A |
INTRINSIC |
|
Pfam:Response_reg
|
134 |
252 |
2.9e-15 |
PFAM |
|
Blast:HDc
|
420 |
481 |
1e-20 |
BLAST |
|
HDc
|
565 |
748 |
3.01e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000067082
AA Change: N540Y
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000070465
Gene: ENSMUSG00000021684
AA Change: N540Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDE8
|
1 |
47 |
1.4e-32 |
PFAM |
|
low complexity region
|
75 |
98 |
N/A |
INTRINSIC |
|
Blast:REC
|
112 |
235 |
6e-45 |
BLAST |
|
PAS
|
249 |
316 |
3.59e-3 |
SMART |
|
Blast:HDc
|
447 |
508 |
1e-20 |
BLAST |
|
HDc
|
592 |
775 |
3.01e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000159608
AA Change: N513Y
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000125191
Gene: ENSMUSG00000021684
AA Change: N513Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDE8
|
1 |
52 |
1.7e-39 |
PFAM |
|
low complexity region
|
75 |
98 |
N/A |
INTRINSIC |
|
Pfam:Response_reg
|
134 |
252 |
2.1e-15 |
PFAM |
|
Blast:HDc
|
420 |
481 |
1e-20 |
BLAST |
|
HDc
|
565 |
666 |
9.37e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000160957
|
| SMART Domains |
Protein: ENSMUSP00000125115
Gene: ENSMUSG00000021684
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
3 |
13 |
N/A |
INTRINSIC |
|
Pfam:Response_reg
|
30 |
148 |
5.7e-16 |
PFAM |
|
Blast:PAS
|
165 |
188 |
1e-7 |
BLAST |
|
Blast:HDc
|
266 |
327 |
1e-21 |
BLAST |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000162153
AA Change: N455Y
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000124704
Gene: ENSMUSG00000021684
AA Change: N455Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Response_reg
|
29 |
147 |
2.6e-15 |
PFAM |
|
PAS
|
164 |
231 |
3.59e-3 |
SMART |
|
Blast:HDc
|
362 |
423 |
1e-20 |
BLAST |
|
HDc
|
507 |
690 |
3.01e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000162292
AA Change: N463Y
PolyPhen 2
Score 0.982 (Sensitivity: 0.75; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000124068
Gene: ENSMUSG00000021684
AA Change: N463Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDE8
|
1 |
52 |
2.1e-39 |
PFAM |
|
low complexity region
|
75 |
98 |
N/A |
INTRINSIC |
|
Pfam:Response_reg
|
134 |
252 |
2.6e-15 |
PFAM |
|
Blast:HDc
|
370 |
431 |
1e-20 |
BLAST |
|
HDc
|
515 |
698 |
3.01e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000162412
AA Change: N405Y
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000124409
Gene: ENSMUSG00000021684
AA Change: N405Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Response_reg
|
29 |
147 |
2.3e-15 |
PFAM |
|
PAS
|
164 |
231 |
3.59e-3 |
SMART |
|
Blast:HDc
|
312 |
373 |
1e-20 |
BLAST |
|
HDc
|
457 |
640 |
3.01e-3 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000172104
AA Change: N505Y
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000128987
Gene: ENSMUSG00000021684
AA Change: N505Y
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDE8
|
1 |
52 |
2.2e-39 |
PFAM |
|
low complexity region
|
75 |
98 |
N/A |
INTRINSIC |
|
Pfam:Response_reg
|
134 |
252 |
2.8e-15 |
PFAM |
|
PAS
|
269 |
336 |
3.59e-3 |
SMART |
|
HDc
|
557 |
740 |
3.01e-3 |
SMART |
|
| Meta Mutation Damage Score |
0.1889 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.8%
|
| Validation Efficiency |
98% (64/65) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased uring corticosterone, decreased serum adrenocorticotropin and decreased sensitivity to a PDE8-selective inhibitor. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 65 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930447C04Rik |
T |
A |
12: 72,956,795 (GRCm39) |
T132S |
possibly damaging |
Het |
| 4933412E24Rik |
A |
G |
15: 59,887,738 (GRCm39) |
V234A |
probably benign |
Het |
| Abtb3 |
T |
A |
10: 85,490,346 (GRCm39) |
L1004Q |
probably damaging |
Het |
| Acsm5 |
A |
G |
7: 119,136,511 (GRCm39) |
T361A |
possibly damaging |
Het |
| Agxt2 |
G |
A |
15: 10,358,753 (GRCm39) |
R24H |
probably benign |
Het |
| Asb16 |
G |
T |
11: 102,159,935 (GRCm39) |
W96L |
probably damaging |
Het |
| Bche |
T |
A |
3: 73,607,969 (GRCm39) |
T486S |
probably benign |
Het |
| Bpnt2 |
C |
A |
4: 4,792,962 (GRCm39) |
G48W |
probably damaging |
Het |
| Cast |
T |
C |
13: 74,875,453 (GRCm39) |
T382A |
probably benign |
Het |
| Cdcp1 |
T |
A |
9: 123,014,119 (GRCm39) |
K218N |
possibly damaging |
Het |
| Ceacam18 |
G |
A |
7: 43,291,308 (GRCm39) |
G250D |
probably damaging |
Het |
| Ciao3 |
T |
A |
17: 25,994,108 (GRCm39) |
V52E |
possibly damaging |
Het |
| Ctnnd2 |
T |
C |
15: 30,905,855 (GRCm39) |
I834T |
possibly damaging |
Het |
| Cyp11b2 |
C |
A |
15: 74,725,840 (GRCm39) |
G136W |
probably damaging |
Het |
| Dis3 |
A |
G |
14: 99,324,925 (GRCm39) |
V502A |
probably damaging |
Het |
| Dnai2 |
T |
A |
11: 114,648,054 (GRCm39) |
M610K |
unknown |
Het |
| Drosha |
G |
A |
15: 12,846,169 (GRCm39) |
V435I |
possibly damaging |
Het |
| Dsc2 |
T |
A |
18: 20,184,236 (GRCm39) |
R51* |
probably null |
Het |
| Ergic2 |
A |
T |
6: 148,096,757 (GRCm39) |
C170S |
probably damaging |
Het |
| Exoc7 |
A |
T |
11: 116,195,688 (GRCm39) |
|
probably null |
Het |
| Fbxw25 |
G |
T |
9: 109,483,660 (GRCm39) |
A184E |
|
Het |
| Gm4846 |
T |
A |
1: 166,314,648 (GRCm39) |
T332S |
probably benign |
Het |
| Greb1l |
T |
A |
18: 10,544,561 (GRCm39) |
M1385K |
probably benign |
Het |
| Grik1 |
T |
C |
16: 87,709,708 (GRCm39) |
N871S |
probably benign |
Het |
| Il11ra1 |
T |
C |
4: 41,765,109 (GRCm39) |
L145P |
probably damaging |
Het |
| Inpp5e |
A |
G |
2: 26,298,104 (GRCm39) |
S166P |
probably benign |
Het |
| Kdm4b |
T |
A |
17: 56,703,333 (GRCm39) |
L676H |
probably damaging |
Het |
| Lrp2 |
T |
A |
2: 69,289,875 (GRCm39) |
K3655* |
probably null |
Het |
| Lrrc74a |
A |
G |
12: 86,787,753 (GRCm39) |
N128S |
probably damaging |
Het |
| Map3k14 |
T |
C |
11: 103,117,848 (GRCm39) |
E648G |
probably damaging |
Het |
| Mbtps1 |
A |
T |
8: 120,269,489 (GRCm39) |
D200E |
probably benign |
Het |
| Mttp |
T |
C |
3: 137,829,546 (GRCm39) |
D114G |
probably benign |
Het |
| Mup13 |
G |
A |
4: 61,182,752 (GRCm39) |
T101M |
probably benign |
Het |
| Neb |
T |
A |
2: 52,096,956 (GRCm39) |
T4953S |
probably benign |
Het |
| Nfasc |
A |
C |
1: 132,562,001 (GRCm39) |
L147R |
probably damaging |
Het |
| Obox6 |
T |
C |
7: 15,567,805 (GRCm39) |
E214G |
probably benign |
Het |
| Opn3 |
T |
C |
1: 175,493,039 (GRCm39) |
N175S |
probably damaging |
Het |
| Or1j12 |
A |
T |
2: 36,342,851 (GRCm39) |
M85L |
probably benign |
Het |
| Or8g22 |
T |
A |
9: 38,958,815 (GRCm39) |
|
probably benign |
Het |
| Or8k22 |
C |
A |
2: 86,163,136 (GRCm39) |
C188F |
possibly damaging |
Het |
| Osbpl3 |
G |
T |
6: 50,323,410 (GRCm39) |
D224E |
probably benign |
Het |
| Osr2 |
A |
G |
15: 35,301,032 (GRCm39) |
D196G |
probably damaging |
Het |
| Pirb |
A |
G |
7: 3,719,177 (GRCm39) |
S571P |
probably benign |
Het |
| Psmc3 |
T |
A |
2: 90,886,275 (GRCm39) |
I163N |
probably damaging |
Het |
| Rapgef4 |
A |
G |
2: 72,038,445 (GRCm39) |
D532G |
probably damaging |
Het |
| Retreg1 |
A |
G |
15: 25,971,684 (GRCm39) |
D208G |
probably benign |
Het |
| Rgsl1 |
T |
G |
1: 153,679,876 (GRCm39) |
|
probably null |
Het |
| Ripk4 |
T |
C |
16: 97,545,157 (GRCm39) |
T497A |
probably benign |
Het |
| Slc30a2 |
T |
A |
4: 134,076,581 (GRCm39) |
|
probably null |
Het |
| Slc6a19 |
T |
C |
13: 73,834,197 (GRCm39) |
D335G |
probably benign |
Het |
| Slco4c1 |
G |
A |
1: 96,799,497 (GRCm39) |
T113M |
probably benign |
Het |
| Speer1c |
T |
C |
5: 10,295,192 (GRCm39) |
N71D |
|
Het |
| Stxbp6 |
A |
G |
12: 44,948,786 (GRCm39) |
F108L |
probably benign |
Het |
| Sulf1 |
T |
A |
1: 12,921,189 (GRCm39) |
|
probably null |
Het |
| Syt16 |
C |
T |
12: 74,313,483 (GRCm39) |
R470C |
probably damaging |
Het |
| Tbc1d19 |
T |
A |
5: 54,029,618 (GRCm39) |
D326E |
probably damaging |
Het |
| Trappc14 |
T |
C |
5: 138,261,839 (GRCm39) |
S86G |
probably benign |
Het |
| Trgv3 |
A |
G |
13: 19,427,188 (GRCm39) |
T24A |
probably benign |
Het |
| Trpm3 |
T |
A |
19: 22,956,048 (GRCm39) |
M1170K |
possibly damaging |
Het |
| Tubgcp6 |
G |
A |
15: 88,987,146 (GRCm39) |
Q1276* |
probably null |
Het |
| Tyrp1 |
A |
G |
4: 80,755,821 (GRCm39) |
K197E |
possibly damaging |
Het |
| Ube3d |
T |
C |
9: 86,322,679 (GRCm39) |
D165G |
probably damaging |
Het |
| Zfp212 |
A |
G |
6: 47,897,678 (GRCm39) |
T7A |
probably benign |
Het |
| Zhx1 |
T |
C |
15: 57,917,758 (GRCm39) |
T163A |
probably benign |
Het |
| Zp2 |
A |
T |
7: 119,734,576 (GRCm39) |
|
probably null |
Het |
|
| Other mutations in Pde8b |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00325:Pde8b
|
APN |
13 |
95,170,875 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01517:Pde8b
|
APN |
13 |
95,237,395 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01736:Pde8b
|
APN |
13 |
95,166,910 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01756:Pde8b
|
APN |
13 |
95,182,895 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01867:Pde8b
|
APN |
13 |
95,237,446 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01939:Pde8b
|
APN |
13 |
95,232,232 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL02026:Pde8b
|
APN |
13 |
95,170,869 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02685:Pde8b
|
APN |
13 |
95,162,628 (GRCm39) |
makesense |
probably null |
|
|
IGL02830:Pde8b
|
APN |
13 |
95,189,409 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL02966:Pde8b
|
APN |
13 |
95,232,156 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03003:Pde8b
|
APN |
13 |
95,178,465 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03064:Pde8b
|
APN |
13 |
95,182,906 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03349:Pde8b
|
APN |
13 |
95,179,551 (GRCm39) |
splice site |
probably benign |
|
|
R0356:Pde8b
|
UTSW |
13 |
95,182,962 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R0464:Pde8b
|
UTSW |
13 |
95,241,206 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0711:Pde8b
|
UTSW |
13 |
95,244,325 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1436:Pde8b
|
UTSW |
13 |
95,162,678 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1467:Pde8b
|
UTSW |
13 |
95,170,680 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1467:Pde8b
|
UTSW |
13 |
95,170,680 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1494:Pde8b
|
UTSW |
13 |
95,184,304 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1546:Pde8b
|
UTSW |
13 |
95,182,951 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1699:Pde8b
|
UTSW |
13 |
95,169,374 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1795:Pde8b
|
UTSW |
13 |
95,178,527 (GRCm39) |
missense |
probably benign |
0.10 |
|
R1879:Pde8b
|
UTSW |
13 |
95,221,723 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R2184:Pde8b
|
UTSW |
13 |
95,162,723 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2223:Pde8b
|
UTSW |
13 |
95,179,955 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2892:Pde8b
|
UTSW |
13 |
95,170,767 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3034:Pde8b
|
UTSW |
13 |
95,359,275 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4204:Pde8b
|
UTSW |
13 |
95,359,053 (GRCm39) |
missense |
probably benign |
0.22 |
|
R4206:Pde8b
|
UTSW |
13 |
95,359,053 (GRCm39) |
missense |
probably benign |
0.22 |
|
R4623:Pde8b
|
UTSW |
13 |
95,178,447 (GRCm39) |
missense |
possibly damaging |
0.69 |
|
R4711:Pde8b
|
UTSW |
13 |
95,166,958 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5133:Pde8b
|
UTSW |
13 |
95,223,250 (GRCm39) |
missense |
probably benign |
0.05 |
|
R5134:Pde8b
|
UTSW |
13 |
95,223,250 (GRCm39) |
missense |
probably benign |
0.05 |
|
R5314:Pde8b
|
UTSW |
13 |
95,223,361 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R5342:Pde8b
|
UTSW |
13 |
95,178,498 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5376:Pde8b
|
UTSW |
13 |
95,162,654 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5806:Pde8b
|
UTSW |
13 |
95,178,548 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5830:Pde8b
|
UTSW |
13 |
95,178,398 (GRCm39) |
missense |
probably benign |
0.01 |
|
R6021:Pde8b
|
UTSW |
13 |
95,162,670 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6035:Pde8b
|
UTSW |
13 |
95,164,105 (GRCm39) |
intron |
probably benign |
|
|
R6035:Pde8b
|
UTSW |
13 |
95,164,105 (GRCm39) |
intron |
probably benign |
|
|
R6129:Pde8b
|
UTSW |
13 |
95,178,467 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6181:Pde8b
|
UTSW |
13 |
95,223,316 (GRCm39) |
missense |
probably benign |
0.36 |
|
R6313:Pde8b
|
UTSW |
13 |
95,178,508 (GRCm39) |
nonsense |
probably null |
|
|
R6849:Pde8b
|
UTSW |
13 |
95,184,307 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R6914:Pde8b
|
UTSW |
13 |
95,223,352 (GRCm39) |
missense |
probably benign |
0.06 |
|
R6999:Pde8b
|
UTSW |
13 |
95,223,342 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R7149:Pde8b
|
UTSW |
13 |
95,223,349 (GRCm39) |
missense |
probably benign |
0.03 |
|
R7483:Pde8b
|
UTSW |
13 |
95,164,251 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7553:Pde8b
|
UTSW |
13 |
95,223,258 (GRCm39) |
missense |
probably benign |
0.21 |
|
R7790:Pde8b
|
UTSW |
13 |
95,170,679 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7802:Pde8b
|
UTSW |
13 |
95,237,446 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7852:Pde8b
|
UTSW |
13 |
95,244,205 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7872:Pde8b
|
UTSW |
13 |
95,223,347 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R7897:Pde8b
|
UTSW |
13 |
95,244,202 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8144:Pde8b
|
UTSW |
13 |
95,359,278 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8792:Pde8b
|
UTSW |
13 |
95,179,534 (GRCm39) |
missense |
probably benign |
|
|
R8850:Pde8b
|
UTSW |
13 |
95,226,793 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8905:Pde8b
|
UTSW |
13 |
95,182,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9252:Pde8b
|
UTSW |
13 |
95,169,424 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9256:Pde8b
|
UTSW |
13 |
95,164,204 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9582:Pde8b
|
UTSW |
13 |
95,169,369 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGAATTCCTGGCTCATGGGC -3'
(R):5'- TTTGTTTCTCATGGCAACCCTAAAG -3'
Sequencing Primer
(F):5'- TCCTGGCTCATGGGCACAAAG -3'
(R):5'- TTTCTCATGGCAACCCTAAAGATAAC -3'
|
| Posted On |
2019-06-26 |
Incidental Mutation