Mutagenetix > Incidental Mutation

Incidental Mutation 'R8766:Or10a3'

667886

Institutional Source

Beutler Lab

Gene Symbol

Or10a3

Ensembl Gene

ENSMUSG00000046431

Gene Name

olfactory receptor family 10 subfamily A member 3

Synonyms

Olfr518, MOR268-5, GA_x6K02T2PBJ9-11211854-11210853

MMRRC Submission

068600-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R8766 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

108479810-108480811 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 108480453 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 120 (M120T)

Ref Sequence

ENSEMBL: ENSMUSP00000151883 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059617] [ENSMUST00000217803]

AlphaFold

Q8VEW1

Predicted Effect

probably benign
Transcript: ENSMUST00000059617
AA Change: M120T

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000050503
Gene: ENSMUSG00000046431
AA Change: M120T

Domain	Start	End	E-Value	Type
low complexity region	11	22	N/A	INTRINSIC
Pfam:7tm_4	50	327	7.1e-60	PFAM
Pfam:7tm_1	60	318	1.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217803
AA Change: M120T

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)

Meta Mutation Damage Score

0.1060

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (49/49)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr10	T	G	12: 71,001,430 (GRCm39)		probably null	Het
Amz1	A	G	5: 140,733,921 (GRCm39)	N166D	probably benign	Het
Armh4	T	A	14: 50,011,497 (GRCm39)	N70I	probably damaging	Het
Asah2	A	G	19: 32,035,280 (GRCm39)	F15S	possibly damaging	Het
Axin2	A	G	11: 108,814,657 (GRCm39)	M182V	probably damaging	Het
Cacna1s	T	C	1: 136,002,881 (GRCm39)	L326P	probably damaging	Het
Ccdc169	T	C	3: 55,058,245 (GRCm39)		probably null	Het
Cct8	A	T	16: 87,285,756 (GRCm39)	V234D	probably damaging	Het
Cdh19	T	C	1: 110,817,844 (GRCm39)	R633G	probably benign	Het
Clca3a1	A	T	3: 144,714,939 (GRCm39)		probably benign	Het
Crat	G	A	2: 30,297,075 (GRCm39)	P293S	probably benign	Het
Crybg3	G	A	16: 59,375,696 (GRCm39)	R139C	probably benign	Het
Ctbs	A	G	3: 146,165,588 (GRCm39)	N301S	possibly damaging	Het
Cyp4x1	T	C	4: 114,967,262 (GRCm39)	H438R	probably damaging	Het
Dglucy	C	A	12: 100,837,706 (GRCm39)	H616Q	probably benign	Het
Dync1i1	T	A	6: 5,767,142 (GRCm39)	L74Q	possibly damaging	Het
Epn1	T	C	7: 5,095,860 (GRCm39)	S167P	possibly damaging	Het
Fbxw28	T	C	9: 109,155,749 (GRCm39)	E341G	probably benign	Het
Gk2	T	A	5: 97,604,110 (GRCm39)	M243L	probably benign	Het
Grhl3	T	A	4: 135,300,724 (GRCm39)	E4D	probably benign	Het
Hsd17b13	T	C	5: 104,125,009 (GRCm39)	K58R	probably benign	Het
Ighd5-7	G	A	12: 113,428,148 (GRCm39)	Q9*	probably null	Het
Izumo1	G	A	7: 45,276,496 (GRCm39)	S353N	probably benign	Het
Klhl31	T	C	9: 77,557,445 (GRCm39)	S54P	possibly damaging	Het
Lonp2	T	C	8: 87,363,198 (GRCm39)	M272T	probably benign	Het
Ltbp1	T	A	17: 75,603,250 (GRCm39)	C682*	probably null	Het
Magi2	A	G	5: 20,400,123 (GRCm39)	K52E	probably benign	Het
Nab2	C	T	10: 127,502,417 (GRCm39)		probably benign	Het
Ncapg2	T	A	12: 116,390,356 (GRCm39)	V435D	probably damaging	Het
Nid2	A	G	14: 19,802,340 (GRCm39)	D87G	probably benign	Het
Or9a7	T	C	6: 40,521,485 (GRCm39)	M143V	probably benign	Het
Pamr1	T	C	2: 102,380,538 (GRCm39)	M1T	probably null	Het
Pard3b	T	C	1: 62,198,637 (GRCm39)	C242R	probably benign	Het
Pcif1	T	A	2: 164,727,346 (GRCm39)	V106E	probably benign	Het
Pitx2	T	A	3: 129,012,223 (GRCm39)	F218I	probably damaging	Het
Ppip5k1	G	T	2: 121,166,919 (GRCm39)	Y877*	probably null	Het
Prkch	A	G	12: 73,749,312 (GRCm39)	N354S	probably benign	Het
Psen2	T	A	1: 180,073,201 (GRCm39)	D40V	probably benign	Het
Rfx7	T	A	9: 72,524,021 (GRCm39)	S404T	possibly damaging	Het
Ryr2	T	A	13: 11,683,855 (GRCm39)	T3070S	probably damaging	Het
Slc24a4	A	G	12: 102,196,711 (GRCm39)	D263G	probably benign	Het
Tox	T	C	4: 6,823,047 (GRCm39)	E90G	probably damaging	Het
Unc13b	C	T	4: 43,174,722 (GRCm39)	S1850L	unknown	Het
Vmn1r5	T	A	6: 56,963,100 (GRCm39)	D258E	possibly damaging	Het
Vmn2r84	C	T	10: 130,222,241 (GRCm39)	V660M	probably damaging	Het
Yju2b	C	T	8: 84,988,423 (GRCm39)	R68H	probably damaging	Het
Zfp592	A	T	7: 80,674,353 (GRCm39)	H439L	probably benign	Het
Zfp775	A	T	6: 48,597,113 (GRCm39)	H329L	probably damaging	Het
Zswim5	T	C	4: 116,816,004 (GRCm39)	L364P	probably damaging	Het

Other mutations in Or10a3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02713:Or10a3	APN	7	108,480,060 (GRCm39)	missense	probably damaging	0.99
IGL02995:Or10a3	APN	7	108,480,198 (GRCm39)	missense	probably damaging	1.00
IGL03162:Or10a3	APN	7	108,480,811 (GRCm39)	start codon destroyed	probably null
IGL03389:Or10a3	APN	7	108,479,982 (GRCm39)	missense	probably damaging	0.99
R0731:Or10a3	UTSW	7	108,480,740 (GRCm39)	missense	probably damaging	1.00
R1669:Or10a3	UTSW	7	108,479,920 (GRCm39)	missense	probably benign	0.00
R2235:Or10a3	UTSW	7	108,480,172 (GRCm39)	missense	probably benign	0.09
R4740:Or10a3	UTSW	7	108,480,689 (GRCm39)	missense	probably benign	0.05
R4902:Or10a3	UTSW	7	108,480,624 (GRCm39)	missense	probably benign	0.00
R5343:Or10a3	UTSW	7	108,480,205 (GRCm39)	missense	possibly damaging	0.87
R6744:Or10a3	UTSW	7	108,480,037 (GRCm39)	missense	probably damaging	0.99
R7157:Or10a3	UTSW	7	108,480,475 (GRCm39)	missense	probably benign	0.03
R7326:Or10a3	UTSW	7	108,480,023 (GRCm39)	missense	probably damaging	1.00
R7713:Or10a3	UTSW	7	108,479,889 (GRCm39)	missense	probably damaging	1.00
R7819:Or10a3	UTSW	7	108,480,610 (GRCm39)	missense	probably damaging	0.99
R7939:Or10a3	UTSW	7	108,480,481 (GRCm39)	missense	probably benign	0.05
R8057:Or10a3	UTSW	7	108,480,571 (GRCm39)	missense	probably damaging	1.00
R8096:Or10a3	UTSW	7	108,480,248 (GRCm39)	nonsense	probably null
R8472:Or10a3	UTSW	7	108,479,973 (GRCm39)	missense	possibly damaging	0.95
R9283:Or10a3	UTSW	7	108,480,289 (GRCm39)	missense	probably benign	0.03
R9570:Or10a3	UTSW	7	108,480,504 (GRCm39)	missense	possibly damaging	0.95
R9763:Or10a3	UTSW	7	108,480,874 (GRCm39)	start gained	probably benign
X0066:Or10a3	UTSW	7	108,480,679 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTCCTTGTGGTCACAGTAGG -3'
(R):5'- GGGAAATGCCACCATTGTTGC -3'

Sequencing Primer
(F):5'- ATGTGGTCTGCACAGTAGCCAC -3'
(R):5'- CTGTCATCCTCCTGGACCAGAG -3'

Posted On

2021-03-08