Mutagenetix > Incidental Mutation

Incidental Mutation 'R9595:Or4f61'

723202

Institutional Source

Beutler Lab

Gene Symbol

Or4f61

Ensembl Gene

ENSMUSG00000074945

Gene Name

olfactory receptor family 4 subfamily F member 61

Synonyms

MOR245-2, Olfr1314, GA_x6K02T2Q125-73139026-73138088

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9595 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111922106-111923044 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111922375 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 224 (T224A)

Ref Sequence

ENSEMBL: ENSMUSP00000146418 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099598] [ENSMUST00000207976]

AlphaFold

A2AVL6

Predicted Effect

probably damaging
Transcript: ENSMUST00000099598
AA Change: T224A

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000097193
Gene: ENSMUSG00000074945
AA Change: T224A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	305	9.2e-42	PFAM
Pfam:7tm_1	41	287	1.6e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000207976
AA Change: T224A

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	C	13: 77,464,566 (GRCm39)	M921T	probably benign	Het
Ankrd33	T	C	15: 101,013,785 (GRCm39)		probably null	Het
Azin2	A	G	4: 128,853,617 (GRCm39)	V80A	probably benign	Het
Capza3	A	T	6: 139,987,712 (GRCm39)	I104F	probably benign	Het
Cd177	A	G	7: 24,451,762 (GRCm39)	L418P	probably damaging	Het
Cd86	A	T	16: 36,441,275 (GRCm39)	V64D	probably damaging	Het
Cfap65	A	G	1: 74,946,537 (GRCm39)	S1352P	probably damaging	Het
Chst10	C	A	1: 38,913,029 (GRCm39)		probably null	Het
Cyp2j13	G	T	4: 95,933,797 (GRCm39)	C362*	probably null	Het
Ddn	T	C	15: 98,705,577 (GRCm39)	T40A	possibly damaging	Het
Dennd2b	C	T	7: 109,155,973 (GRCm39)	R259Q	probably damaging	Het
Fam47e	G	C	5: 92,726,395 (GRCm39)	R111P	probably benign	Het
Fermt3	C	T	19: 6,979,619 (GRCm39)	V505M	probably damaging	Het
Flnc	A	G	6: 29,433,720 (GRCm39)	H88R	probably benign	Het
Glyat	A	G	19: 12,623,728 (GRCm39)	D48G	probably damaging	Het
Hdac5	A	G	11: 102,096,129 (GRCm39)	V348A	probably benign	Het
Ighv2-3	T	C	12: 113,575,084 (GRCm39)	K24E	probably benign	Het
Iglv1	A	G	16: 18,903,948 (GRCm39)	V57A	possibly damaging	Het
Il16	G	T	7: 83,322,273 (GRCm39)	Y347*	probably null	Het
Il20rb	G	A	9: 100,368,311 (GRCm39)	T24M	possibly damaging	Het
Itgae	A	G	11: 73,016,182 (GRCm39)	D797G	probably damaging	Het
Lrrc37a	T	A	11: 103,392,552 (GRCm39)	T958S	probably benign	Het
Mall	G	T	2: 127,571,751 (GRCm39)	Y12*	probably null	Het
N4bp3	C	T	11: 51,536,932 (GRCm39)	R47Q	probably damaging	Het
Nacad	T	C	11: 6,551,790 (GRCm39)	D467G	probably damaging	Het
Or12k8	C	A	2: 36,975,204 (GRCm39)	K185N	possibly damaging	Het
Or13a18	T	C	7: 140,190,939 (GRCm39)	S279P	probably damaging	Het
Or1af1	T	A	2: 37,110,281 (GRCm39)	I260N	probably damaging	Het
Or7a40	G	A	16: 16,491,470 (GRCm39)	A125V	probably damaging	Het
Or7e177	A	G	9: 20,211,661 (GRCm39)	N56S	probably damaging	Het
Osbpl8	A	G	10: 111,108,909 (GRCm39)	E397G	probably damaging	Het
Pcmt1	A	T	10: 7,524,817 (GRCm39)	I111K	possibly damaging	Het
Pcnx1	T	A	12: 81,965,688 (GRCm39)	H90Q		Het
Pde4dip	C	T	3: 97,602,207 (GRCm39)		probably null	Het
Peli2	T	A	14: 48,493,846 (GRCm39)	V356D	probably damaging	Het
Phyhipl	G	T	10: 70,395,512 (GRCm39)	C231*	probably null	Het
Pirb	A	T	7: 3,722,406 (GRCm39)	S146T	possibly damaging	Het
Rac1	G	A	5: 143,513,643 (GRCm39)		probably benign	Het
Sh3glb2	C	T	2: 30,236,672 (GRCm39)	R230Q	probably damaging	Het
Slc38a8	C	A	8: 120,209,403 (GRCm39)	C390F	probably benign	Het
Slc39a8	G	T	3: 135,592,688 (GRCm39)	L454F	possibly damaging	Het
Slit1	A	C	19: 41,637,851 (GRCm39)	I314S	probably damaging	Het
Son	T	G	16: 91,454,241 (GRCm39)	M996R	possibly damaging	Het
Sp4	A	G	12: 118,262,690 (GRCm39)	V452A	possibly damaging	Het
Tmf1	C	T	6: 97,135,457 (GRCm39)	D940N	probably damaging	Het
Tns3	G	A	11: 8,401,142 (GRCm39)	T1052M	possibly damaging	Het
Trim33	C	T	3: 103,259,350 (GRCm39)	P1013L	probably damaging	Het
Vmn1r175	A	T	7: 23,508,508 (GRCm39)	S40T	probably damaging	Het
Vmn1r43	G	A	6: 89,846,877 (GRCm39)	T203M	probably damaging	Het
Wdr49	C	T	3: 75,265,747 (GRCm39)	C233Y	probably damaging	Het
Wrn	T	C	8: 33,758,961 (GRCm39)	E999G	probably benign	Het
Yju2b	C	T	8: 84,988,400 (GRCm39)	V76I	probably damaging	Het
Zfp142	A	T	1: 74,611,462 (GRCm39)	C778S	probably damaging	Het

Other mutations in Or4f61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00972:Or4f61	APN	2	111,922,439 (GRCm39)	missense	probably damaging	0.99
IGL01701:Or4f61	APN	2	111,922,851 (GRCm39)	missense	possibly damaging	0.69
IGL02085:Or4f61	APN	2	111,922,869 (GRCm39)	missense	probably damaging	1.00
IGL02156:Or4f61	APN	2	111,922,361 (GRCm39)	missense	probably benign	0.12
IGL02266:Or4f61	APN	2	111,922,588 (GRCm39)	missense	probably benign	0.05
IGL02396:Or4f61	APN	2	111,922,812 (GRCm39)	missense	probably benign	0.20
IGL02602:Or4f61	APN	2	111,922,906 (GRCm39)	missense	probably benign	0.00
IGL03130:Or4f61	APN	2	111,922,166 (GRCm39)	missense	probably benign
R0452:Or4f61	UTSW	2	111,922,981 (GRCm39)	nonsense	probably null
R1498:Or4f61	UTSW	2	111,922,938 (GRCm39)	missense	probably benign	0.40
R1514:Or4f61	UTSW	2	111,922,381 (GRCm39)	missense	probably benign	0.01
R1852:Or4f61	UTSW	2	111,922,192 (GRCm39)	missense	probably benign	0.03
R2118:Or4f61	UTSW	2	111,922,675 (GRCm39)	missense	probably benign	0.02
R2219:Or4f61	UTSW	2	111,922,752 (GRCm39)	missense	probably damaging	0.99
R2357:Or4f61	UTSW	2	111,922,743 (GRCm39)	missense	possibly damaging	0.69
R3743:Or4f61	UTSW	2	111,922,965 (GRCm39)	missense	probably benign	0.33
R4692:Or4f61	UTSW	2	111,923,026 (GRCm39)	missense	probably damaging	1.00
R5092:Or4f61	UTSW	2	111,922,452 (GRCm39)	missense	possibly damaging	0.94
R5150:Or4f61	UTSW	2	111,922,880 (GRCm39)	missense	possibly damaging	0.95
R5230:Or4f61	UTSW	2	111,922,734 (GRCm39)	missense	probably benign	0.12
R5991:Or4f61	UTSW	2	111,922,960 (GRCm39)	missense	probably benign	0.30
R7894:Or4f61	UTSW	2	111,922,822 (GRCm39)	missense	probably benign
R8991:Or4f61	UTSW	2	111,922,682 (GRCm39)	missense	probably damaging	1.00
R9206:Or4f61	UTSW	2	111,922,410 (GRCm39)	missense	probably benign	0.12
Z1177:Or4f61	UTSW	2	111,922,929 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- TGATTCCTCAGTGTGTAGATCAC -3'
(R):5'- TGGCCTTATGCATTCTCTGG -3'

Sequencing Primer
(F):5'- TCACAGGGTTCAGAAAAGGAGTACC -3'
(R):5'- GCATTCTCTGGTTCAATTTGTTTAC -3'

Posted On

2022-08-09