Mutagenetix > Incidental Mutation

Incidental Mutation 'R1132:Zdhhc25'

94762

Institutional Source

Beutler Lab

Gene Symbol

Zdhhc25

Ensembl Gene

ENSMUSG00000054117

Gene Name

zinc finger, DHHC domain containing 25

Synonyms

1700030J15Rik

MMRRC Submission

039205-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R1132 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

88484505-88485872 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88484926 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 87 (L87P)

Ref Sequence

ENSEMBL: ENSMUSP00000136289 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066949]

AlphaFold

Q810M4

Predicted Effect

probably damaging
Transcript: ENSMUST00000066949
AA Change: L87P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000136289
Gene: ENSMUSG00000054117
AA Change: L87P

Domain	Start	End	E-Value	Type
transmembrane domain	35	55	N/A	INTRINSIC
transmembrane domain	65	87	N/A	INTRINSIC
Pfam:zf-DHHC	105	235	2.4e-32	PFAM

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.3%
20x: 92.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apba1	T	C	19: 23,894,917 (GRCm39)	V451A	possibly damaging	Het
Atxn2l	CCAGCAGCAGCAGCAGCAGC	CCAGCAGCAGCAGCAGC	7: 126,093,420 (GRCm39)		probably benign	Het
C3	C	T	17: 57,514,531 (GRCm39)		probably null	Het
Car9	G	T	4: 43,512,439 (GRCm39)		probably null	Het
Cd163	G	T	6: 124,286,055 (GRCm39)	G202*	probably null	Het
Cdk8	A	G	5: 146,236,625 (GRCm39)	T347A	probably benign	Het
Cep170	C	A	1: 176,577,603 (GRCm39)	R1257L	probably damaging	Het
Cib1	A	G	7: 79,877,778 (GRCm39)	F168S	probably damaging	Het
Cntnap5c	G	A	17: 58,601,351 (GRCm39)	G833D	probably damaging	Het
Dhx37	A	T	5: 125,498,103 (GRCm39)	I702N	probably damaging	Het
Dnah3	T	C	7: 119,538,227 (GRCm39)	K3586R	possibly damaging	Het
Fbxo31	ACGGCGCGGCG	ACGGCGCGGCGCGGCG	8: 122,279,015 (GRCm39)		probably null	Het
Fbxo31	CGCGG	CGCGGAGCGG	8: 122,279,019 (GRCm39)		probably null	Het
Fhod3	TGAGGAGGAGGAGGAGGA	TGAGGAGGAGGAGGA	18: 25,153,722 (GRCm39)		probably benign	Het
Gaa	T	C	11: 119,175,885 (GRCm39)	S953P	probably damaging	Het
Inpp5j	T	C	11: 3,452,305 (GRCm39)	E315G	possibly damaging	Het
Itsn2	T	C	12: 4,708,464 (GRCm39)	Y840H	probably damaging	Het
Kif1a	T	C	1: 92,983,743 (GRCm39)	E653G	probably damaging	Het
Loxhd1	T	C	18: 77,517,639 (GRCm39)	V1829A	possibly damaging	Het
Myh8	C	T	11: 67,187,957 (GRCm39)	Q910*	probably null	Het
Or12d12	T	C	17: 37,610,423 (GRCm39)	R297G	probably benign	Het
Or14a257	A	T	7: 86,138,425 (GRCm39)	F111L	probably benign	Het
Or14j4	G	T	17: 37,921,333 (GRCm39)	T103K	possibly damaging	Het
Or2ag17	A	T	7: 106,389,758 (GRCm39)	I150N	possibly damaging	Het
Prdm4	A	G	10: 85,735,145 (GRCm39)	S666P	probably damaging	Het
Rad50	T	C	11: 53,585,788 (GRCm39)	K331E	possibly damaging	Het
Rbbp6	A	G	7: 122,599,336 (GRCm39)		probably benign	Het
Selenbp1	C	G	3: 94,844,644 (GRCm39)	I100M	probably benign	Het
Skint6	A	G	4: 112,755,296 (GRCm39)		probably null	Het
Stac3	T	C	10: 127,343,128 (GRCm39)	S208P	probably benign	Het
Tfap2a	T	C	13: 40,874,867 (GRCm39)		probably null	Het
Trhde	T	C	10: 114,248,383 (GRCm39)	K939E	possibly damaging	Het
Vmn1r22	T	C	6: 57,877,826 (GRCm39)	I50M	probably benign	Het
Vmn1r39	C	T	6: 66,781,428 (GRCm39)	V260I	probably benign	Het
Zfp202	T	C	9: 40,122,318 (GRCm39)	L360P	probably benign	Het

Other mutations in Zdhhc25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01999:Zdhhc25	APN	15	88,485,217 (GRCm39)	missense	probably damaging	0.98
IGL02448:Zdhhc25	APN	15	88,485,045 (GRCm39)	missense	probably damaging	1.00
IGL02576:Zdhhc25	APN	15	88,485,472 (GRCm39)	missense	probably benign	0.01
R0001:Zdhhc25	UTSW	15	88,485,112 (GRCm39)	missense	probably benign	0.00
R0394:Zdhhc25	UTSW	15	88,485,123 (GRCm39)	missense	probably damaging	0.96
R0622:Zdhhc25	UTSW	15	88,485,310 (GRCm39)	missense	probably damaging	0.98
R1116:Zdhhc25	UTSW	15	88,484,823 (GRCm39)	missense	probably benign	0.25
R1854:Zdhhc25	UTSW	15	88,484,689 (GRCm39)	missense	probably benign
R1994:Zdhhc25	UTSW	15	88,485,027 (GRCm39)	missense	probably benign	0.00
R2102:Zdhhc25	UTSW	15	88,484,962 (GRCm39)	missense	probably benign	0.10
R3749:Zdhhc25	UTSW	15	88,485,226 (GRCm39)	missense	probably benign	0.00
R5144:Zdhhc25	UTSW	15	88,485,259 (GRCm39)	missense	probably damaging	0.98
R5220:Zdhhc25	UTSW	15	88,485,365 (GRCm39)	nonsense	probably null
R9573:Zdhhc25	UTSW	15	88,485,307 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAACCAACGCTGCTGGTTCATATTG -3'
(R):5'- CATTATCGCCACCAGGAGGAGAAAG -3'

Sequencing Primer
(F):5'- TGTTCCGGGACCTGCTTATA -3'
(R):5'- GGTTGTCTTCACCAATGCAG -3'

Posted On

2014-01-05