Incidental Mutation 'IGL01942:Gpr65'
ID180873
Institutional Source Australian Phenomics Network (link to record)
Gene Symbol Gpr65
Ensembl Gene ENSMUSG00000021886
Gene NameG-protein coupled receptor 65
SynonymsTDAG8, Gpcr25, Dig1
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #IGL01942
Quality Score
Status
Chromosome12
Chromosomal Location98268635-98276644 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 98275715 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 209 (Y209F)
Ref Sequence ENSEMBL: ENSMUSP00000074581 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075072]
Predicted Effect possibly damaging
Transcript: ENSMUST00000075072
AA Change: Y209F

PolyPhen 2 Score 0.928 (Sensitivity: 0.81; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000074581
Gene: ENSMUSG00000021886
AA Change: Y209F

DomainStartEndE-ValueType
Pfam:7tm_1 33 290 1.9e-41 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000218404
Predicted Effect noncoding transcript
Transcript: ENSMUST00000219320
Coding Region Coverage
Validation Efficiency
MGI Phenotype PHENOTYPE: Homozygous mutant mice have thymocytes and splenocytes that are insensitive to pH-dependent cAMP production. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 38 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcc6 G A 7: 45,986,573 R1017W possibly damaging Het
Arhgef26 A G 3: 62,340,094 R200G probably benign Het
Arid4b A T 13: 14,136,164 probably benign Het
Atp1a2 A C 1: 172,286,309 S369A probably benign Het
Bag3 T A 7: 128,546,300 D546E probably benign Het
Bcl6b T A 11: 70,226,743 Y379F probably damaging Het
Cbln2 T C 18: 86,716,325 V136A probably benign Het
Ccnf A T 17: 24,242,320 D120E probably benign Het
Cd14 T A 18: 36,725,640 H254L possibly damaging Het
Chd3 A G 11: 69,350,105 probably null Het
Csf2rb G A 15: 78,340,492 R150H probably benign Het
Dhx35 T C 2: 158,831,864 L405P probably damaging Het
Dpf1 G A 7: 29,316,502 C383Y probably damaging Het
Dse T A 10: 34,155,993 Q345L probably benign Het
Gnaz A G 10: 75,014,874 M244V probably damaging Het
Inpp5f T C 7: 128,667,769 I281T probably damaging Het
Kntc1 T C 5: 123,778,267 F721S probably damaging Het
Mapk8ip2 G A 15: 89,457,017 probably null Het
Med24 G A 11: 98,709,682 R646W probably damaging Het
Nup98 A T 7: 102,194,711 F102Y probably damaging Het
Olfr1260 G T 2: 89,977,978 V67L probably benign Het
Olfr150 A T 9: 39,737,666 M284L possibly damaging Het
Olfr354 A G 2: 36,907,857 M304V probably benign Het
Pgap3 T C 11: 98,397,954 Y125C probably damaging Het
Rasl12 T C 9: 65,408,362 V96A probably damaging Het
Rbm20 G A 19: 53,813,443 M127I probably damaging Het
Rnf114 T A 2: 167,512,626 probably null Het
Serpinb1b C T 13: 33,085,311 T9I possibly damaging Het
Setd1b T A 5: 123,163,426 F16I possibly damaging Het
Slc25a28 A G 19: 43,664,508 F238S probably damaging Het
Slc2a2 T C 3: 28,705,803 V30A probably damaging Het
Slc35f4 T C 14: 49,525,505 probably benign Het
Socs4 T A 14: 47,290,650 C347* probably null Het
Spock1 C T 13: 57,430,328 E367K probably damaging Het
Ubap2 C T 4: 41,251,608 R8H probably benign Het
Vmn1r44 T A 6: 89,893,824 M41K probably benign Het
Vmn2r-ps159 C T 4: 156,338,254 noncoding transcript Het
Zfp575 T C 7: 24,585,815 T134A possibly damaging Het
Other mutations in Gpr65
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00435:Gpr65 APN 12 98275556 missense probably damaging 1.00
IGL00717:Gpr65 APN 12 98276055 missense probably benign 0.09
IGL01643:Gpr65 APN 12 98275754 missense probably damaging 1.00
IGL02023:Gpr65 APN 12 98275868 missense probably benign 0.25
IGL02803:Gpr65 APN 12 98275210 missense probably damaging 1.00
R1343:Gpr65 UTSW 12 98275629 missense probably benign 0.00
R1520:Gpr65 UTSW 12 98275175 missense probably benign 0.01
R1771:Gpr65 UTSW 12 98276000 missense probably damaging 0.96
R1812:Gpr65 UTSW 12 98275742 missense probably damaging 1.00
R2261:Gpr65 UTSW 12 98275235 missense probably damaging 1.00
R2263:Gpr65 UTSW 12 98275235 missense probably damaging 1.00
R5720:Gpr65 UTSW 12 98275102 missense probably damaging 1.00
Posted On2014-05-07