Incidental Mutation 'R1812:Gpr65'
ID202499
Institutional Source Beutler Lab
Gene Symbol Gpr65
Ensembl Gene ENSMUSG00000021886
Gene NameG-protein coupled receptor 65
SynonymsTDAG8, Gpcr25, Dig1
MMRRC Submission 039840-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R1812 (G1)
Quality Score225
Status Not validated
Chromosome12
Chromosomal Location98268635-98276644 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to T at 98275742 bp
ZygosityHeterozygous
Amino Acid Change Threonine to Methionine at position 218 (T218M)
Ref Sequence ENSEMBL: ENSMUSP00000074581 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075072]
Predicted Effect probably damaging
Transcript: ENSMUST00000075072
AA Change: T218M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000074581
Gene: ENSMUSG00000021886
AA Change: T218M

DomainStartEndE-ValueType
Pfam:7tm_1 33 290 1.9e-41 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000218404
Predicted Effect noncoding transcript
Transcript: ENSMUST00000219320
Coding Region Coverage
  • 1x: 97.3%
  • 3x: 96.3%
  • 10x: 93.2%
  • 20x: 85.7%
Validation Efficiency 100% (1/1)
MGI Phenotype PHENOTYPE: Homozygous mutant mice have thymocytes and splenocytes that are insensitive to pH-dependent cAMP production. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 72 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017B05Rik G A 9: 57,257,457 Q545* probably null Het
Aadacl2 T C 3: 60,025,077 C338R probably damaging Het
Abcb4 G A 5: 8,928,578 probably null Het
Adam17 T C 12: 21,361,767 D41G probably damaging Het
Angptl7 T A 4: 148,498,083 I119F probably damaging Het
Arid1b T A 17: 5,337,029 S1586T probably benign Het
Arid4b C T 13: 14,195,429 A1170V probably damaging Het
Atad5 A G 11: 80,133,047 T1659A probably damaging Het
Bub1b T G 2: 118,632,421 D754E probably benign Het
Cdkn1a T C 17: 29,098,565 V53A probably benign Het
Chsy1 T G 7: 66,171,817 V600G probably benign Het
Cntrl G A 2: 35,149,469 V561M probably damaging Het
Col6a5 A G 9: 105,928,054 C1218R unknown Het
Crhr1 T C 11: 104,169,147 L140P probably damaging Het
Cyb5b CAGAG CAG 8: 107,170,388 probably null Het
Cyp3a59 A T 5: 146,102,811 Q298L probably damaging Het
Dctn1 A G 6: 83,192,518 E638G possibly damaging Het
Ddx41 A G 13: 55,535,954 I88T probably benign Het
Diaph1 G T 18: 37,891,018 P589Q unknown Het
Dip2b C A 15: 100,198,938 probably null Het
Dscaml1 G A 9: 45,751,286 probably null Het
Dync1h1 C T 12: 110,662,900 A4246V possibly damaging Het
E330009J07Rik A G 6: 40,409,431 I288T probably benign Het
Epb41l1 T C 2: 156,496,511 I158T probably damaging Het
Fat1 A G 8: 45,036,803 Y3584C probably damaging Het
Fxyd5 T A 7: 31,037,930 probably null Het
Gapvd1 T A 2: 34,725,064 K336* probably null Het
Gm9637 G A 14: 19,402,395 noncoding transcript Het
Gsdma3 T C 11: 98,632,393 V203A probably damaging Het
Helb T A 10: 120,089,566 K969* probably null Het
Hipk2 G A 6: 38,698,163 A1188V probably benign Het
Itpk1 T A 12: 102,574,058 E255D probably benign Het
Kif21a T C 15: 90,971,766 D596G possibly damaging Het
Kif5a A T 10: 127,242,010 I405N probably benign Het
Klk11 T A 7: 43,777,755 probably null Het
Luzp1 T A 4: 136,542,331 L622M probably benign Het
Macf1 T A 4: 123,432,024 I5227F probably damaging Het
Mapk10 A C 5: 102,913,262 S470A probably damaging Het
Morc2a C T 11: 3,685,831 T897I probably damaging Het
Nmbr A G 10: 14,760,539 probably null Het
Nosip T A 7: 45,076,574 M214K probably damaging Het
Olfr1136 A G 2: 87,693,103 F260L probably benign Het
Olfr159 G T 4: 43,770,230 Y260* probably null Het
Olfr553 A T 7: 102,614,370 N206K possibly damaging Het
Olfr569 T C 7: 102,888,078 Y25C probably benign Het
Pik3c2a A T 7: 116,417,664 V286E probably damaging Het
Ppfia4 T A 1: 134,324,573 I388F probably benign Het
Ppm1f A G 16: 16,917,787 H289R probably damaging Het
Ptprz1 T A 6: 22,959,712 D69E probably benign Het
Rad54b A T 4: 11,612,770 T801S probably damaging Het
Ramp1 A T 1: 91,196,857 N47Y probably damaging Het
Rnf32 T A 5: 29,206,260 H181Q possibly damaging Het
Rpa2 T C 4: 132,768,685 F6L probably benign Het
Ryr2 C A 13: 11,560,586 R4842L probably damaging Het
Scn11a G T 9: 119,780,865 C972* probably null Het
Setd2 C T 9: 110,550,102 T995I probably damaging Het
Slc39a7 A G 17: 34,028,815 L471P probably damaging Het
Slc6a21 C G 7: 45,282,947 S350R probably damaging Het
Slx4ip A G 2: 137,068,195 N300S probably benign Het
Spef2 G A 15: 9,679,349 P634L probably damaging Het
Stk32b G A 5: 37,466,758 A215V probably damaging Het
Svil A T 18: 5,097,545 Y1676F probably damaging Het
Tanc1 G A 2: 59,791,679 V381M probably damaging Het
Tanc2 T C 11: 105,886,386 F797L probably benign Het
Tas2r120 T G 6: 132,657,601 C215W probably benign Het
Tenm4 G A 7: 96,895,940 D2388N probably damaging Het
Thsd4 C T 9: 60,056,937 S64N probably damaging Het
Thsd7b C T 1: 129,758,610 R630C probably damaging Het
Top3a A G 11: 60,759,362 I145T probably damaging Het
Vmn2r78 A G 7: 86,920,787 D171G probably benign Het
Vmn2r82 A G 10: 79,379,212 E343G probably benign Het
Wdr70 T C 15: 8,079,179 D161G probably benign Het
Other mutations in Gpr65
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00435:Gpr65 APN 12 98275556 missense probably damaging 1.00
IGL00717:Gpr65 APN 12 98276055 missense probably benign 0.09
IGL01643:Gpr65 APN 12 98275754 missense probably damaging 1.00
IGL01942:Gpr65 APN 12 98275715 missense possibly damaging 0.93
IGL02023:Gpr65 APN 12 98275868 missense probably benign 0.25
IGL02803:Gpr65 APN 12 98275210 missense probably damaging 1.00
R1343:Gpr65 UTSW 12 98275629 missense probably benign 0.00
R1520:Gpr65 UTSW 12 98275175 missense probably benign 0.01
R1771:Gpr65 UTSW 12 98276000 missense probably damaging 0.96
R2261:Gpr65 UTSW 12 98275235 missense probably damaging 1.00
R2263:Gpr65 UTSW 12 98275235 missense probably damaging 1.00
R5720:Gpr65 UTSW 12 98275102 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCTTCTGTGGAAAGATGAAACG -3'
(R):5'- CACAGTTTAGACTCGTCAGGG -3'

Sequencing Primer
(F):5'- AACGAGTGTTGAATATTGTGACTCG -3'
(R):5'- AGGGCTACTGTGACTCTGTACAC -3'
Posted On2014-06-23