Mutagenetix > Incidental Mutation

Incidental Mutation 'R2444:Vmn2r82'

249923

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

040402-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R2444 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

79192425-79232600 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 79213702 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 96 (H96L)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170596
AA Change: H96L

PolyPhen 2 Score 0.650 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: H96L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.0%
20x: 94.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610008E11Rik	A	T	10: 78,904,561 (GRCm39)	D112E	possibly damaging	Het
Abca15	A	G	7: 119,965,120 (GRCm39)	Y794C	probably damaging	Het
Bicd2	T	C	13: 49,532,500 (GRCm39)	V362A	probably benign	Het
Cep126	G	A	9: 8,101,307 (GRCm39)	T409M	probably damaging	Het
Cep131	A	G	11: 119,961,321 (GRCm39)	F610S	probably damaging	Het
Cfap61	T	C	2: 145,877,239 (GRCm39)		probably null	Het
Chd1l	A	G	3: 97,497,882 (GRCm39)	Y320H	probably damaging	Het
Dnajc30	A	G	5: 135,093,439 (GRCm39)	D112G	probably damaging	Het
Dync2i1	T	C	12: 116,196,289 (GRCm39)	D486G	possibly damaging	Het
Fam184a	A	C	10: 53,517,045 (GRCm39)	L410R	probably damaging	Het
Fat2	T	C	11: 55,172,799 (GRCm39)	N2638S	probably damaging	Het
Fgf15	A	G	7: 144,453,429 (GRCm39)	D134G	probably benign	Het
Flywch2	A	T	17: 23,996,024 (GRCm39)	S124R	possibly damaging	Het
Gabra5	G	A	7: 57,058,623 (GRCm39)	T375I	probably benign	Het
Gpat3	C	T	5: 101,005,039 (GRCm39)	P58L	probably benign	Het
Hltf	T	A	3: 20,118,071 (GRCm39)	N44K	possibly damaging	Het
Hoatz	A	G	9: 51,011,298 (GRCm39)		probably null	Het
Kcnb2	A	T	1: 15,779,791 (GRCm39)	N221I	probably benign	Het
Marco	G	A	1: 120,422,499 (GRCm39)	T61M	probably damaging	Het
Med13	A	G	11: 86,222,786 (GRCm39)	I180T	probably damaging	Het
Mei1	C	T	15: 81,997,142 (GRCm39)	T626M	probably damaging	Het
Mtnr1a	C	T	8: 45,540,695 (GRCm39)	Q219*	probably null	Het
Nav3	A	G	10: 109,600,776 (GRCm39)	S1284P	probably benign	Het
Or1j10	T	C	2: 36,267,625 (GRCm39)	V279A	possibly damaging	Het
Or7g30	T	A	9: 19,352,311 (GRCm39)	I34K	possibly damaging	Het
Pcdh9	T	A	14: 94,124,227 (GRCm39)	T648S	probably benign	Het
Proz	A	T	8: 13,111,027 (GRCm39)		probably benign	Het
Rec114	G	T	9: 58,567,602 (GRCm39)	A128E	probably damaging	Het
Rspry1	G	T	8: 95,349,735 (GRCm39)	G41V	probably damaging	Het
Sbf2	A	T	7: 109,929,905 (GRCm39)	M1388K	probably benign	Het
Spice1	C	T	16: 44,186,931 (GRCm39)	Q143*	probably null	Het
Tcstv3	A	T	13: 120,779,365 (GRCm39)	K88M	probably damaging	Het
Terb2	T	A	2: 122,023,788 (GRCm39)		probably null	Het
Tmx3	A	T	18: 90,558,307 (GRCm39)	K453M	probably damaging	Het
Tnc	T	C	4: 63,933,200 (GRCm39)	Y688C	probably damaging	Het
Tshz2	T	G	2: 169,726,726 (GRCm39)	S441A	probably benign	Het
Usp45	A	T	4: 21,817,528 (GRCm39)	M399L	probably benign	Het
Wls	G	A	3: 159,612,867 (GRCm39)	R261Q	probably damaging	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79,192,581 (GRCm39)	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79,214,691 (GRCm39)	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79,213,906 (GRCm39)	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79,215,057 (GRCm39)	missense	probably benign
IGL02112:Vmn2r82	APN	10	79,231,833 (GRCm39)	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79,192,542 (GRCm39)	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79,215,205 (GRCm39)	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79,213,678 (GRCm39)	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79,217,149 (GRCm39)	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79,192,643 (GRCm39)	splice site	probably null
IGL03349:Vmn2r82	APN	10	79,213,703 (GRCm39)	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79,232,460 (GRCm39)	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79,232,339 (GRCm39)	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79,217,129 (GRCm39)	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79,214,634 (GRCm39)	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79,232,391 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79,213,739 (GRCm39)	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79,232,325 (GRCm39)	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79,215,201 (GRCm39)	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79,214,702 (GRCm39)	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79,192,578 (GRCm39)	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79,215,253 (GRCm39)	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79,215,046 (GRCm39)	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79,232,344 (GRCm39)	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79,231,890 (GRCm39)	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79,214,680 (GRCm39)	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79,231,813 (GRCm39)	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79,192,519 (GRCm39)	missense	probably benign
R2442:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R2857:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79,231,914 (GRCm39)	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79,214,874 (GRCm39)	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79,192,548 (GRCm39)	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79,214,641 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79,214,589 (GRCm39)	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79,215,010 (GRCm39)	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79,231,921 (GRCm39)	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79,192,491 (GRCm39)	missense	probably null	0.01
R5601:Vmn2r82	UTSW	10	79,232,025 (GRCm39)	missense	probably damaging	1.00
R5635:Vmn2r82	UTSW	10	79,214,652 (GRCm39)	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R6340:Vmn2r82	UTSW	10	79,231,727 (GRCm39)	missense	probably benign	0.00
R6474:Vmn2r82	UTSW	10	79,214,871 (GRCm39)	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79,214,605 (GRCm39)	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79,215,268 (GRCm39)	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79,232,452 (GRCm39)	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79,192,464 (GRCm39)	missense	probably benign	0.00
R7362:Vmn2r82	UTSW	10	79,232,451 (GRCm39)	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79,232,276 (GRCm39)	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79,217,087 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79,231,842 (GRCm39)	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79,232,345 (GRCm39)	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79,232,079 (GRCm39)	missense	possibly damaging	0.74
R8158:Vmn2r82	UTSW	10	79,213,636 (GRCm39)	missense	probably benign	0.12
R8324:Vmn2r82	UTSW	10	79,214,727 (GRCm39)	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79,217,036 (GRCm39)	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79,213,894 (GRCm39)	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79,232,541 (GRCm39)	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79,232,539 (GRCm39)	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79,214,768 (GRCm39)	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79,213,641 (GRCm39)	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79,214,936 (GRCm39)	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79,214,880 (GRCm39)	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79,192,456 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,232,369 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,192,429 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TGTGGGTTCTAAGCAAACACAAC -3'
(R):5'- GCACTCACCTGAGGAAATTTG -3'

Sequencing Primer
(F):5'- CCATTCAAAGTATAGAACTAGTGCAC -3'
(R):5'- GCACTCACCTGAGGAAATTTGTAAAG -3'

Posted On

2014-11-12