Mutagenetix > Incidental Mutation

Incidental Mutation 'R2910:Vmn2r69'

261187

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r69

Ensembl Gene

ENSMUSG00000091006

Gene Name

vomeronasal 2, receptor 69

Synonyms

MMRRC Submission

040497-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R2910 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85055584-85064884 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 85055918 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Aspartic acid at position 740 (A740D)

Ref Sequence

ENSEMBL: ENSMUSP00000132726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171213]

AlphaFold

G3XA45

Predicted Effect

probably damaging
Transcript: ENSMUST00000171213
AA Change: A740D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132726
Gene: ENSMUSG00000091006
AA Change: A740D

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.3e-28	PFAM
Pfam:NCD3G	507	559	1.8e-20	PFAM
Pfam:7tm_3	592	827	3.2e-54	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207880

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

96% (49/51)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf3	A	G	16: 20,378,982 (GRCm39)	T645A	probably damaging	Het
Acap1	A	G	11: 69,777,902 (GRCm39)		probably benign	Het
Adgrv1	G	A	13: 81,705,238 (GRCm39)	A1524V	possibly damaging	Het
Agbl1	A	G	7: 76,069,586 (GRCm39)	N121D	probably benign	Het
Ahnak	A	G	19: 8,989,018 (GRCm39)	D3434G	probably damaging	Het
Ankrd11	A	T	8: 123,635,537 (GRCm39)	D32E	probably damaging	Het
Asxl1	T	C	2: 153,242,959 (GRCm39)	S1170P	probably benign	Het
Atp8b3	G	A	10: 80,355,746 (GRCm39)	S1322F	possibly damaging	Het
Car15	G	A	16: 17,656,006 (GRCm39)		probably benign	Het
Cep104	T	A	4: 154,079,884 (GRCm39)		probably null	Het
Cnnm1	A	G	19: 43,458,086 (GRCm39)	I633V	possibly damaging	Het
Cog8	A	T	8: 107,780,853 (GRCm39)	V135E	probably benign	Het
Cts6	A	G	13: 61,344,215 (GRCm39)	V279A	probably damaging	Het
Ddx1	C	T	12: 13,281,441 (GRCm39)		probably null	Het
Dock2	A	G	11: 34,182,910 (GRCm39)		probably benign	Het
Ero1b	T	A	13: 12,615,178 (GRCm39)	D336E	probably damaging	Het
F11	C	G	8: 45,694,486 (GRCm39)	*625S	probably null	Het
F5	A	G	1: 164,032,389 (GRCm39)	M1779V	probably benign	Het
Fam227a	A	T	15: 79,520,935 (GRCm39)	D296E	possibly damaging	Het
Fbxo38	T	C	18: 62,652,878 (GRCm39)	D523G	probably benign	Het
Ggt1	G	A	10: 75,416,430 (GRCm39)	V275M	probably benign	Het
Gm379	A	C	X: 107,708,371 (GRCm39)	F43V	possibly damaging	Het
Grhl3	T	C	4: 135,286,457 (GRCm39)	I75V	probably benign	Het
Iqcm	A	T	8: 76,441,404 (GRCm39)	I226F	probably benign	Het
Kcnq1	T	G	7: 142,979,699 (GRCm39)	L615R	probably damaging	Het
Lcp2	G	A	11: 34,018,970 (GRCm39)		probably null	Het
Lypd8l	G	A	11: 58,499,252 (GRCm39)	Q189*	probably null	Het
Mbtps1	A	G	8: 120,272,776 (GRCm39)	I123T	possibly damaging	Het
Muc5ac	C	T	7: 141,361,378 (GRCm39)	T1563I	probably damaging	Het
Odf2l	A	C	3: 144,830,084 (GRCm39)	I19L	probably benign	Het
Or5ak23	T	A	2: 85,244,695 (GRCm39)	H176L	probably damaging	Het
Or5h25	C	T	16: 58,930,544 (GRCm39)	R143H	probably benign	Het
Pigr	A	G	1: 130,777,270 (GRCm39)	D692G	probably damaging	Het
Pkd1l3	A	G	8: 110,394,268 (GRCm39)		probably benign	Het
Plekhg4	TAGTCGATGCCCGAGTC	TAGTC	8: 106,103,084 (GRCm39)		probably benign	Het
Reep2	T	C	18: 34,978,743 (GRCm39)		probably null	Het
Rubcnl	C	T	14: 75,278,248 (GRCm39)	T344I	probably benign	Het
Sema6d	C	A	2: 124,506,957 (GRCm39)	P941T	probably damaging	Het
Shcbp1l	C	A	1: 153,304,372 (GRCm39)	L144I	probably damaging	Het
Slc7a1	T	C	5: 148,289,067 (GRCm39)	E60G	probably benign	Het
Snx2	C	T	18: 53,332,946 (GRCm39)	P207S	probably damaging	Het
Spata31	T	G	13: 65,068,250 (GRCm39)	S133A	probably benign	Het
Tac1	A	G	6: 7,559,097 (GRCm39)		probably null	Het
Tfpi	A	C	2: 84,274,437 (GRCm39)	V184G	possibly damaging	Het
Tmeff1	C	A	4: 48,614,961 (GRCm39)	N139K	possibly damaging	Het
Tnxb	A	G	17: 34,891,424 (GRCm39)	D589G	probably damaging	Het
Trpc1	C	A	9: 95,631,895 (GRCm39)	A16S	probably benign	Het
Vmn2r106	A	C	17: 20,498,946 (GRCm39)	L322V	probably damaging	Het

Other mutations in Vmn2r69

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r69	APN	7	85,055,739 (GRCm39)	missense	probably benign
IGL01457:Vmn2r69	APN	7	85,055,836 (GRCm39)	missense	possibly damaging	0.87
IGL01760:Vmn2r69	APN	7	85,056,072 (GRCm39)	missense	possibly damaging	0.90
IGL01834:Vmn2r69	APN	7	85,061,576 (GRCm39)	missense	probably damaging	1.00
IGL02001:Vmn2r69	APN	7	85,056,434 (GRCm39)	missense	probably benign	0.05
IGL02057:Vmn2r69	APN	7	85,060,990 (GRCm39)	missense	possibly damaging	0.93
IGL02289:Vmn2r69	APN	7	85,056,054 (GRCm39)	missense	probably damaging	1.00
IGL02472:Vmn2r69	APN	7	85,058,960 (GRCm39)	missense	probably benign	0.01
IGL02478:Vmn2r69	APN	7	85,055,889 (GRCm39)	missense	probably damaging	1.00
IGL02554:Vmn2r69	APN	7	85,059,014 (GRCm39)	missense	probably damaging	1.00
IGL02723:Vmn2r69	APN	7	85,059,416 (GRCm39)	missense	probably damaging	1.00
R0526:Vmn2r69	UTSW	7	85,060,711 (GRCm39)	missense	probably damaging	1.00
R0560:Vmn2r69	UTSW	7	85,058,922 (GRCm39)	critical splice donor site	probably null
R0909:Vmn2r69	UTSW	7	85,055,873 (GRCm39)	missense	probably benign	0.00
R0976:Vmn2r69	UTSW	7	85,056,108 (GRCm39)	missense	probably damaging	1.00
R1158:Vmn2r69	UTSW	7	85,059,058 (GRCm39)	splice site	probably benign
R1459:Vmn2r69	UTSW	7	85,055,908 (GRCm39)	nonsense	probably null
R1482:Vmn2r69	UTSW	7	85,056,082 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn2r69	UTSW	7	85,060,891 (GRCm39)	missense	probably damaging	1.00
R2016:Vmn2r69	UTSW	7	85,056,493 (GRCm39)	missense	probably damaging	0.98
R2108:Vmn2r69	UTSW	7	85,059,404 (GRCm39)	missense	probably benign
R2571:Vmn2r69	UTSW	7	85,064,764 (GRCm39)	missense	probably benign
R2920:Vmn2r69	UTSW	7	85,060,973 (GRCm39)	missense	probably benign	0.08
R3708:Vmn2r69	UTSW	7	85,061,029 (GRCm39)	missense	probably damaging	0.98
R3710:Vmn2r69	UTSW	7	85,055,601 (GRCm39)	missense	probably benign
R4757:Vmn2r69	UTSW	7	85,061,575 (GRCm39)	missense	probably damaging	0.99
R4823:Vmn2r69	UTSW	7	85,060,508 (GRCm39)	missense	probably benign	0.21
R4870:Vmn2r69	UTSW	7	85,060,793 (GRCm39)	missense	possibly damaging	0.93
R4918:Vmn2r69	UTSW	7	85,055,967 (GRCm39)	missense	probably benign	0.06
R5022:Vmn2r69	UTSW	7	85,060,367 (GRCm39)	missense	possibly damaging	0.72
R5174:Vmn2r69	UTSW	7	85,064,739 (GRCm39)	missense	possibly damaging	0.92
R5200:Vmn2r69	UTSW	7	85,055,717 (GRCm39)	missense	probably damaging	1.00
R5278:Vmn2r69	UTSW	7	85,060,991 (GRCm39)	missense	probably benign	0.02
R5643:Vmn2r69	UTSW	7	85,056,404 (GRCm39)	missense	probably damaging	0.98
R5996:Vmn2r69	UTSW	7	85,061,117 (GRCm39)	splice site	probably null
R6083:Vmn2r69	UTSW	7	85,055,711 (GRCm39)	missense	probably damaging	1.00
R6140:Vmn2r69	UTSW	7	85,060,657 (GRCm39)	missense	probably damaging	0.99
R6306:Vmn2r69	UTSW	7	85,064,799 (GRCm39)	missense	probably benign	0.04
R6330:Vmn2r69	UTSW	7	85,060,835 (GRCm39)	missense	probably benign
R6380:Vmn2r69	UTSW	7	85,061,067 (GRCm39)	missense	probably benign
R6466:Vmn2r69	UTSW	7	85,056,378 (GRCm39)	missense	probably benign	0.01
R6542:Vmn2r69	UTSW	7	85,060,413 (GRCm39)	nonsense	probably null
R6583:Vmn2r69	UTSW	7	85,059,017 (GRCm39)	missense	probably benign
R6623:Vmn2r69	UTSW	7	85,056,309 (GRCm39)	missense	possibly damaging	0.84
R6709:Vmn2r69	UTSW	7	85,061,069 (GRCm39)	missense	probably benign	0.03
R6732:Vmn2r69	UTSW	7	85,060,351 (GRCm39)	missense	probably benign	0.00
R6741:Vmn2r69	UTSW	7	85,061,724 (GRCm39)	missense	probably benign	0.01
R7070:Vmn2r69	UTSW	7	85,060,688 (GRCm39)	missense	probably damaging	0.98
R7234:Vmn2r69	UTSW	7	85,056,315 (GRCm39)	missense	probably benign	0.22
R7323:Vmn2r69	UTSW	7	85,060,972 (GRCm39)	missense	possibly damaging	0.95
R7427:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7428:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7453:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7532:Vmn2r69	UTSW	7	85,059,622 (GRCm39)	missense	probably benign	0.36
R7556:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7562:Vmn2r69	UTSW	7	85,056,420 (GRCm39)	missense	probably benign
R7592:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7708:Vmn2r69	UTSW	7	85,061,755 (GRCm39)	missense	possibly damaging	0.87
R7803:Vmn2r69	UTSW	7	85,056,324 (GRCm39)	missense	probably benign	0.00
R7960:Vmn2r69	UTSW	7	85,055,973 (GRCm39)	missense	probably benign
R7966:Vmn2r69	UTSW	7	85,060,762 (GRCm39)	missense	possibly damaging	0.81
R8071:Vmn2r69	UTSW	7	85,055,713 (GRCm39)	nonsense	probably null
R8237:Vmn2r69	UTSW	7	85,060,340 (GRCm39)	missense	probably benign	0.02
R8347:Vmn2r69	UTSW	7	85,064,838 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r69	UTSW	7	85,055,783 (GRCm39)	missense	probably damaging	1.00
R8795:Vmn2r69	UTSW	7	85,064,883 (GRCm39)	start codon destroyed	probably null	0.94
R8831:Vmn2r69	UTSW	7	85,059,018 (GRCm39)	nonsense	probably null
R8856:Vmn2r69	UTSW	7	85,061,663 (GRCm39)	missense	probably benign	0.00
R8998:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R8999:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R9161:Vmn2r69	UTSW	7	85,056,177 (GRCm39)	missense	possibly damaging	0.88
R9228:Vmn2r69	UTSW	7	85,064,697 (GRCm39)	missense	probably benign	0.01
R9494:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	missense	probably damaging	1.00
R9494:Vmn2r69	UTSW	7	85,056,084 (GRCm39)	missense	probably benign	0.08
R9541:Vmn2r69	UTSW	7	85,056,209 (GRCm39)	missense	probably benign
R9620:Vmn2r69	UTSW	7	85,061,504 (GRCm39)	missense	probably benign	0.10
Z1176:Vmn2r69	UTSW	7	85,055,696 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAACCATAGTCTTGCCTTTGG -3'
(R):5'- GCATTCACTGTGGCTACCTC -3'

Sequencing Primer
(F):5'- CCATAGTCTTGCCTTTGGTGCTG -3'
(R):5'- CTGGCATTCAAGATAACTGTTCCAGG -3'

Posted On

2015-01-23