Mutagenetix > Incidental Mutation

Incidental Mutation 'R4520:Vmn2r84'

334186

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

041763-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R4520 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

130221669-130230110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 130222391 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 610 (T610S)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

AlphaFold

D3YWE3

Predicted Effect

probably damaging
Transcript: ENSMUST00000094502
AA Change: T610S

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: T610S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,776,027 (GRCm39)	T1739A	probably damaging	Het
Ascc3	A	G	10: 50,536,766 (GRCm39)	N700D	probably benign	Het
Ccdc88c	G	T	12: 100,879,591 (GRCm39)	S1843R	possibly damaging	Het
Cel	C	T	2: 28,447,980 (GRCm39)	V349M	probably benign	Het
Cspp1	A	G	1: 10,204,452 (GRCm39)	T1127A	probably benign	Het
Cyp2b10	T	C	7: 25,610,982 (GRCm39)	V113A	probably benign	Het
Cyp2c50	A	G	19: 40,079,133 (GRCm39)	T159A	probably benign	Het
Degs1	T	C	1: 182,104,373 (GRCm39)	D304G	possibly damaging	Het
Esr2	G	A	12: 76,214,323 (GRCm39)	P43S	possibly damaging	Het
Fbxo41	A	G	6: 85,461,024 (GRCm39)	I228T	probably damaging	Het
Gm5444	A	G	13: 4,884,225 (GRCm39)		noncoding transcript	Het
Golga3	T	A	5: 110,351,617 (GRCm39)	L790*	probably null	Het
Gtpbp6	T	C	5: 110,255,725 (GRCm39)	T105A	probably benign	Het
Ido2	T	C	8: 25,066,194 (GRCm39)	E24G	probably damaging	Het
Ihh	T	A	1: 74,990,109 (GRCm39)	I89F	probably damaging	Het
Krt13	A	G	11: 100,010,174 (GRCm39)	M269T	probably damaging	Het
Marf1	T	C	16: 13,950,530 (GRCm39)	H952R	probably damaging	Het
Mga	T	A	2: 119,778,579 (GRCm39)	F2041L	possibly damaging	Het
Msmo1	A	C	8: 65,173,557 (GRCm39)		probably benign	Het
Mterf1a	A	G	5: 3,940,992 (GRCm39)	V292A	probably damaging	Het
Naxe	A	G	3: 87,965,289 (GRCm39)		probably null	Het
Ntng1	A	G	3: 109,842,312 (GRCm39)	S154P	probably damaging	Het
Or51h5	A	T	7: 102,577,764 (GRCm39)	I310F	probably benign	Het
Or56b1	T	A	7: 104,285,376 (GRCm39)	V165E	probably damaging	Het
Or8h7	T	C	2: 86,721,363 (GRCm39)	D52G	probably benign	Het
Otog	T	A	7: 45,890,477 (GRCm39)		probably benign	Het
Pigo	A	T	4: 43,020,301 (GRCm39)	H880Q	probably benign	Het
Plce1	T	C	19: 38,512,763 (GRCm39)	S21P	possibly damaging	Het
Pole	T	A	5: 110,445,790 (GRCm39)	D555E	probably damaging	Het
Prkag2	A	G	5: 25,071,169 (GRCm39)	V403A	probably damaging	Het
Psg28	T	A	7: 18,156,826 (GRCm39)	M470L	probably benign	Het
Rpgrip1	A	G	14: 52,389,746 (GRCm39)	T1138A	probably benign	Het
Rpl10l	T	C	12: 66,330,512 (GRCm39)	D207G	probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Serpinb9c	T	C	13: 33,335,840 (GRCm39)		probably null	Het
Sh2d6	T	C	6: 72,495,936 (GRCm39)	N101D	possibly damaging	Het
Shisa8	C	G	15: 82,096,163 (GRCm39)	V151L	possibly damaging	Het
Slc13a1	T	C	6: 24,134,512 (GRCm39)	T124A	probably benign	Het
Slc6a3	G	A	13: 73,688,975 (GRCm39)	V100M	possibly damaging	Het
Sspn	A	G	6: 145,907,033 (GRCm39)	T79A	probably benign	Het
Tlr8	C	T	X: 166,026,171 (GRCm39)	R893H	probably damaging	Het
Tnrc18	T	C	5: 142,717,905 (GRCm39)	K2183R	unknown	Het
Trim15	T	C	17: 37,177,242 (GRCm39)	I139M	probably benign	Het
Trmt13	A	T	3: 116,375,262 (GRCm39)		probably null	Het
Zcchc14	A	T	8: 122,335,834 (GRCm39)		probably benign	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130,227,094 (GRCm39)	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130,221,964 (GRCm39)	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130,225,141 (GRCm39)	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130,222,148 (GRCm39)	missense	probably benign
IGL01911:Vmn2r84	APN	10	130,222,277 (GRCm39)	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130,221,755 (GRCm39)	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130,229,935 (GRCm39)	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130,227,881 (GRCm39)	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130,226,617 (GRCm39)	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130,227,356 (GRCm39)	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130,229,995 (GRCm39)	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130,223,861 (GRCm39)	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130,222,556 (GRCm39)	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130,221,784 (GRCm39)	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130,230,062 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130,222,588 (GRCm39)	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130,227,877 (GRCm39)	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130,221,991 (GRCm39)	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130,226,984 (GRCm39)	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130,223,725 (GRCm39)	splice site	probably null
R1295:Vmn2r84	UTSW	10	130,225,008 (GRCm39)	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130,227,859 (GRCm39)	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130,225,137 (GRCm39)	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130,227,349 (GRCm39)	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130,221,938 (GRCm39)	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130,221,878 (GRCm39)	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130,227,100 (GRCm39)	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130,227,940 (GRCm39)	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130,222,392 (GRCm39)	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130,230,036 (GRCm39)	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130,226,777 (GRCm39)	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R4051:Vmn2r84	UTSW	10	130,226,767 (GRCm39)	missense	probably damaging	1.00
R4061:Vmn2r84	UTSW	10	130,221,898 (GRCm39)	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130,227,238 (GRCm39)	missense	probably damaging	1.00
R4190:Vmn2r84	UTSW	10	130,227,163 (GRCm39)	nonsense	probably null
R4584:Vmn2r84	UTSW	10	130,226,582 (GRCm39)	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130,221,809 (GRCm39)	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130,229,973 (GRCm39)	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130,222,417 (GRCm39)	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130,221,971 (GRCm39)	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130,221,863 (GRCm39)	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130,225,064 (GRCm39)	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130,221,754 (GRCm39)	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130,222,114 (GRCm39)	missense	probably damaging	1.00
R6286:Vmn2r84	UTSW	10	130,226,737 (GRCm39)	missense	possibly damaging	0.65
R6580:Vmn2r84	UTSW	10	130,225,110 (GRCm39)	missense	possibly damaging	0.93
R6607:Vmn2r84	UTSW	10	130,226,731 (GRCm39)	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130,222,147 (GRCm39)	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130,225,136 (GRCm39)	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130,226,876 (GRCm39)	missense	possibly damaging	0.90
R7075:Vmn2r84	UTSW	10	130,226,941 (GRCm39)	missense	probably damaging	0.99
R7225:Vmn2r84	UTSW	10	130,222,552 (GRCm39)	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130,222,279 (GRCm39)	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130,225,077 (GRCm39)	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130,227,119 (GRCm39)	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130,226,738 (GRCm39)	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130,227,037 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130,230,100 (GRCm39)	missense	probably benign	0.28
R8766:Vmn2r84	UTSW	10	130,222,241 (GRCm39)	missense	probably damaging	0.98
R8821:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8831:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8970:Vmn2r84	UTSW	10	130,222,244 (GRCm39)	missense	probably damaging	0.98
R9164:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R9190:Vmn2r84	UTSW	10	130,226,843 (GRCm39)	missense	probably benign	0.03
R9261:Vmn2r84	UTSW	10	130,229,976 (GRCm39)	missense	probably benign	0.00
R9310:Vmn2r84	UTSW	10	130,227,993 (GRCm39)	missense	possibly damaging	0.81
R9434:Vmn2r84	UTSW	10	130,221,745 (GRCm39)	missense	possibly damaging	0.65
R9613:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r84	UTSW	10	130,227,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTTCCTCCTGGAGTAGTG -3'
(R):5'- TGTGTGAGATGTCCAGATGATC -3'

Sequencing Primer
(F):5'- GACCACAGTTATTGTTTTGGCCAAC -3'
(R):5'- GAGATGTCCAGATGATCAATATGCC -3'

Posted On

2015-08-18