Mutagenetix > Incidental Mutation

Incidental Mutation 'R5386:Ncam1'

425237

Institutional Source

Beutler Lab

Gene Symbol

Ncam1

Ensembl Gene

ENSMUSG00000039542

Gene Name

neural cell adhesion molecule 1

Synonyms

NCAM, NCAM-1, NCAM-120, E-NCAM, CD56, NCAM-140, NCAM-180

Accession Numbers

Essential gene?

Probably essential (E-score: 0.928) question?

Stock #

R5386 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

49413436-49710225 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 49476174 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 305 (V305G)

Ref Sequence

ENSEMBL: ENSMUSP00000142275 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000114476] [ENSMUST00000166811] [ENSMUST00000192584] [ENSMUST00000193547]

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000068730

Predicted Effect

probably damaging
Transcript: ENSMUST00000114476
AA Change: V305G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110120
Gene: ENSMUSG00000039542
AA Change: V305G

Domain	Start	End	E-Value	Type
IGc2	32	103	2.88e-4	SMART
IGc2	130	196	6.35e-6	SMART
IGc2	226	295	6.38e-20	SMART
IGc2	321	393	4.12e-14	SMART
IGc2	418	487	9.7e-11	SMART
FN3	501	586	4.77e-8	SMART
FN3	602	683	6.97e-1	SMART
low complexity region	711	725	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000166811
AA Change: V305G

SMART Domains

Protein: ENSMUSP00000130668
Gene: ENSMUSG00000039542
AA Change: V305G

Domain	Start	End	E-Value	Type
IGc2	32	103	2.88e-4	SMART
IGc2	130	196	6.35e-6	SMART
IGc2	226	295	6.38e-20	SMART
IGc2	321	393	4.12e-14	SMART
IGc2	418	487	9.7e-11	SMART
FN3	501	586	4.77e-8	SMART
FN3	602	683	6.97e-1	SMART
transmembrane domain	706	728	N/A	INTRINSIC
low complexity region	797	809	N/A	INTRINSIC
low complexity region	814	830	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000192584
AA Change: V305G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000141700
Gene: ENSMUSG00000039542
AA Change: V305G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
IGc2	32	103	1.2e-6	SMART
IGc2	130	196	2.6e-8	SMART
IGc2	226	295	2.6e-22	SMART
IGc2	321	393	1.6e-16	SMART
IGc2	418	487	4e-13	SMART
FN3	501	586	2.4e-10	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000193547
AA Change: V305G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000142275
Gene: ENSMUSG00000039542
AA Change: V305G

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
IGc2	32	103	2.88e-4	SMART
IGc2	130	196	6.35e-6	SMART
IGc2	226	295	6.38e-20	SMART
IGc2	321	393	4.12e-14	SMART
IGc2	418	487	9.7e-11	SMART
FN3	501	586	4.77e-8	SMART
FN3	602	683	6.97e-1	SMART
transmembrane domain	706	728	N/A	INTRINSIC
low complexity region	797	809	N/A	INTRINSIC
low complexity region	814	830	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000194252
AA Change: V262G

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215070

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215465

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216483

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
PHENOTYPE: Homozygous mutants show impairment in Morris water maze test, reduced brain and olfactory bulb size, hypoplasic corticospinal tract, abnormally distributed anterior pituitary cell types, and morphological and functional defects of neuromuscular junctions. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 134 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc12	T	G	8: 87,244,118 (GRCm39)	K1012Q	possibly damaging	Het
Afmid	G	A	11: 117,718,968 (GRCm39)	G33R	probably benign	Het
Agbl3	G	T	6: 34,776,131 (GRCm39)	W207C	probably damaging	Het
Ajuba	T	C	14: 54,807,855 (GRCm39)	Y459C	probably damaging	Het
Ak2	T	C	4: 128,901,965 (GRCm39)	S213P	probably benign	Het
Alk	T	A	17: 72,182,007 (GRCm39)	N1339Y	probably damaging	Het
Angpt1	T	C	15: 42,301,761 (GRCm39)	S416G	probably damaging	Het
Ank2	C	T	3: 126,775,582 (GRCm39)	V854M	probably benign	Het
Ankrd61	G	T	5: 143,828,482 (GRCm39)	N122K	possibly damaging	Het
Armc9	T	A	1: 86,126,011 (GRCm39)	L34Q	probably null	Het
Aspg	T	A	12: 112,089,466 (GRCm39)	V418E	probably benign	Het
Baz1b	C	T	5: 135,266,913 (GRCm39)	R1241C	probably damaging	Het
Bclaf1	T	C	10: 20,201,338 (GRCm39)	V201A	possibly damaging	Het
C1ql3	A	T	2: 13,009,169 (GRCm39)	D225E	probably damaging	Het
Capn9	A	G	8: 125,332,279 (GRCm39)	T417A	possibly damaging	Het
Card14	C	T	11: 119,208,115 (GRCm39)	R62C	probably damaging	Het
Cc2d2a	A	G	5: 43,887,383 (GRCm39)	N1271S	probably benign	Het
Ccpg1	G	A	9: 72,920,326 (GRCm39)	S647N	probably benign	Het
Cdv3	T	C	9: 103,232,429 (GRCm39)	K133R	possibly damaging	Het
Cep128	A	C	12: 90,966,345 (GRCm39)	S1087R	probably benign	Het
Cep70	T	A	9: 99,163,128 (GRCm39)	L325Q	probably damaging	Het
Chit1	T	G	1: 134,077,192 (GRCm39)	F332V	probably damaging	Het
Chodl	C	A	16: 78,743,585 (GRCm39)	T219K	probably damaging	Het
Cntn4	T	C	6: 106,158,765 (GRCm39)	L10P	possibly damaging	Het
Copg1	G	T	6: 87,867,189 (GRCm39)	M87I	possibly damaging	Het
Cplane1	G	A	15: 8,223,897 (GRCm39)	G887R	probably damaging	Het
Cyp3a59	A	G	5: 146,022,578 (GRCm39)	Y28C	probably benign	Het
Dchs1	A	C	7: 105,407,236 (GRCm39)	V2119G	probably damaging	Het
Dedd	T	C	1: 171,165,951 (GRCm39)	L23P	probably damaging	Het
Dmxl2	A	G	9: 54,286,041 (GRCm39)	S2715P	probably benign	Het
Dnah9	T	C	11: 65,920,182 (GRCm39)	N2237S	probably damaging	Het
Drosha	A	G	15: 12,842,207 (GRCm39)	I337V	probably benign	Het
Duox2	A	G	2: 122,125,617 (GRCm39)	V330A	probably benign	Het
Dusp11	T	C	6: 85,924,587 (GRCm39)	*322W	probably null	Het
Dusp8	T	A	7: 141,643,730 (GRCm39)	Q61L	possibly damaging	Het
Ears2	G	C	7: 121,643,600 (GRCm39)	T426S	probably benign	Het
Elmo1	T	C	13: 20,784,380 (GRCm39)	Y646H	probably benign	Het
Eps15	T	A	4: 109,178,422 (GRCm39)	I220K	possibly damaging	Het
Faap100	T	A	11: 120,268,458 (GRCm39)	E105V	possibly damaging	Het
Fam171a2	C	A	11: 102,328,693 (GRCm39)	V689L	possibly damaging	Het
Fam91a1	G	A	15: 58,320,243 (GRCm39)	S645N	probably benign	Het
Fancg	G	A	4: 43,007,076 (GRCm39)	Q234*	probably null	Het
Garre1	A	G	7: 33,941,813 (GRCm39)	F120L	probably damaging	Het
Gid4	T	A	11: 60,323,268 (GRCm39)		probably null	Het
Gm7361	A	C	5: 26,463,903 (GRCm39)	T53P	probably benign	Het
Golgb1	T	A	16: 36,732,677 (GRCm39)	C641*	probably null	Het
Gon4l	T	A	3: 88,765,803 (GRCm39)	M409K	probably benign	Het
Gpatch8	A	T	11: 102,399,053 (GRCm39)		probably null	Het
Gpr156	T	A	16: 37,768,671 (GRCm39)	V64E	possibly damaging	Het
Grid2	T	A	6: 63,908,089 (GRCm39)	I243K	probably damaging	Het
Gucy2g	G	A	19: 55,203,548 (GRCm39)	A750V	probably damaging	Het
Hdac5	T	C	11: 102,092,967 (GRCm39)	E590G	possibly damaging	Het
Herc3	T	A	6: 58,851,263 (GRCm39)	M504K	probably damaging	Het
Hif1a	A	G	12: 73,990,867 (GRCm39)	E713G	probably benign	Het
Hmx3	A	G	7: 131,146,033 (GRCm39)	D247G	probably damaging	Het
Hoxd11	G	T	2: 74,513,163 (GRCm39)	E143*	probably null	Het
Ifitm3	T	C	7: 140,590,554 (GRCm39)	N2S	probably benign	Het
Il17f	T	C	1: 20,848,181 (GRCm39)	Q99R	probably benign	Het
Itga6	T	A	2: 71,671,494 (GRCm39)	S341R	probably damaging	Het
Itgam	A	G	7: 127,707,152 (GRCm39)	N661S	probably benign	Het
Jag1	A	T	2: 136,937,464 (GRCm39)	H303Q	possibly damaging	Het
Kcnh8	T	A	17: 53,033,023 (GRCm39)	N103K	probably benign	Het
Kdm6b	T	C	11: 69,291,636 (GRCm39)		probably benign	Het
Keg1	A	C	19: 12,691,902 (GRCm39)	N63T	probably damaging	Het
Klf12	T	A	14: 100,137,595 (GRCm39)	H317L	probably damaging	Het
Lrp1	A	T	10: 127,427,983 (GRCm39)	V530E	probably damaging	Het
Mrgpra6	A	G	7: 46,838,629 (GRCm39)	C190R	probably damaging	Het
Myo1a	G	T	10: 127,541,766 (GRCm39)	E102*	probably null	Het
Napa	A	T	7: 15,850,397 (GRCm39)	E265D	probably benign	Het
Nek8	T	C	11: 78,061,263 (GRCm39)		probably null	Het
Or10ag53	C	T	2: 87,082,827 (GRCm39)	P182L	probably benign	Het
Or11g24	T	A	14: 50,662,846 (GRCm39)	V290E	possibly damaging	Het
Or4a70	G	T	2: 89,324,481 (GRCm39)	Y58*	probably null	Het
Or4f52	T	C	2: 111,061,539 (GRCm39)	T200A	probably benign	Het
Or52a20	T	C	7: 103,366,562 (GRCm39)	F254L	probably benign	Het
Or5ak4	T	C	2: 85,161,979 (GRCm39)	T88A	probably benign	Het
Or5h23	T	A	16: 58,906,253 (GRCm39)	M198L	probably benign	Het
Or8c11	A	T	9: 38,289,281 (GRCm39)	I29F	probably benign	Het
Or8g2b	A	G	9: 39,751,126 (GRCm39)	Y132C	possibly damaging	Het
Pabpc4	A	G	4: 123,188,790 (GRCm39)	Q417R	probably benign	Het
Panx3	A	T	9: 37,580,320 (GRCm39)	M11K	probably damaging	Het
Pcbp1	C	T	6: 86,502,471 (GRCm39)	E143K	probably damaging	Het
Pdcd7	G	A	9: 65,265,974 (GRCm39)	W477*	probably null	Het
Pi4k2a	G	A	19: 42,078,954 (GRCm39)	S5N	probably damaging	Het
Plag1	T	A	4: 3,904,075 (GRCm39)	Q372L	probably benign	Het
Plce1	C	A	19: 38,748,535 (GRCm39)	N1755K	probably damaging	Het
Pld4	G	T	12: 112,730,422 (GRCm39)	E102*	probably null	Het
Pnlip	A	G	19: 58,668,039 (GRCm39)	N345S	probably benign	Het
Ppm1e	A	T	11: 87,249,377 (GRCm39)	L118Q	possibly damaging	Het
Prkra	T	G	2: 76,469,622 (GRCm39)	T146P	probably damaging	Het
Prpf8	A	G	11: 75,386,625 (GRCm39)	D1038G	probably damaging	Het
Prss51	T	A	14: 64,334,543 (GRCm39)	V108E	probably damaging	Het
Prxl2b	T	A	4: 154,983,462 (GRCm39)	M1L	probably benign	Het
Ptch1	A	G	13: 63,692,857 (GRCm39)	Y181H	probably damaging	Het
Reln	A	G	5: 22,244,527 (GRCm39)	V817A	probably benign	Het
Rictor	C	A	15: 6,818,985 (GRCm39)	Q1403K	probably benign	Het
Rims1	T	A	1: 22,482,469 (GRCm39)	I882F	probably damaging	Het
Rnf19a	A	G	15: 36,242,185 (GRCm39)	V618A	probably benign	Het
Ryr1	A	T	7: 28,816,841 (GRCm39)	I65N	probably damaging	Het
S1pr2	T	C	9: 20,878,890 (GRCm39)	T313A	probably benign	Het
Serpine2	A	T	1: 79,799,004 (GRCm39)	Y83*	probably null	Het
Sfi1	A	ATCTTCCCAAAGCCAGTGC	11: 3,103,384 (GRCm39)		probably benign	Het
Sgip1	T	C	4: 102,772,256 (GRCm39)	V215A	probably benign	Het
Sgsm3	T	C	15: 80,892,200 (GRCm39)	V256A	probably benign	Het
Shroom4	T	A	X: 6,497,523 (GRCm39)	C894*	probably null	Het
Slc14a2	T	A	18: 78,229,055 (GRCm39)	D306V	possibly damaging	Het
Slc28a2b	C	A	2: 122,353,259 (GRCm39)	L480I	probably benign	Het
Slc4a10	A	T	2: 62,120,402 (GRCm39)	E843V	probably damaging	Het
Smyd4	T	A	11: 75,280,982 (GRCm39)	C152S	probably damaging	Het
Snx10	T	C	6: 51,552,952 (GRCm39)	Y32H	probably damaging	Het
Sorl1	T	C	9: 41,968,580 (GRCm39)	T558A	possibly damaging	Het
Spata31d1b	G	A	13: 59,866,866 (GRCm39)	C1338Y	possibly damaging	Het
Sppl2c	T	A	11: 104,078,127 (GRCm39)	I309K	possibly damaging	Het
Stard9	A	G	2: 120,531,111 (GRCm39)	E2456G	probably damaging	Het
Stx1b	A	T	7: 127,414,575 (GRCm39)	D16E	probably benign	Het
Tecpr2	T	C	12: 110,881,887 (GRCm39)	V152A	probably damaging	Het
Tedc1	T	C	12: 113,120,302 (GRCm39)	V47A	probably benign	Het
Tep1	A	T	14: 51,105,774 (GRCm39)	L82Q	probably damaging	Het
Tgm4	A	G	9: 122,885,559 (GRCm39)	Y367C	probably damaging	Het
Tle1	C	T	4: 72,060,081 (GRCm39)	V258M	probably damaging	Het
Tm9sf1	C	T	14: 55,880,301 (GRCm39)	G32D	possibly damaging	Het
Tmco2	A	G	4: 120,963,181 (GRCm39)	L106P	probably damaging	Het
Tmem131	A	T	1: 36,911,639 (GRCm39)	C103S	possibly damaging	Het
Tmprss7	T	A	16: 45,489,891 (GRCm39)	I444F	possibly damaging	Het
Trank1	T	C	9: 111,191,470 (GRCm39)	V493A	probably benign	Het
Trim80	A	T	11: 115,338,843 (GRCm39)	T558S	probably benign	Het
Trmt6	C	A	2: 132,650,703 (GRCm39)	A302S	probably benign	Het
Ttc17	A	T	2: 94,133,985 (GRCm39)	W1067R	probably damaging	Het
Tulp4	T	A	17: 6,286,568 (GRCm39)	V1532D	probably damaging	Het
Ubqlnl	T	G	7: 103,798,424 (GRCm39)	I358L	probably benign	Het
Ulk3	T	A	9: 57,498,023 (GRCm39)	I108N	possibly damaging	Het
Vps13b	A	T	15: 35,640,674 (GRCm39)		probably null	Het
Vwa3a	A	G	7: 120,389,365 (GRCm39)	K68E	possibly damaging	Het
Zfp746	G	T	6: 48,041,110 (GRCm39)	H538N	possibly damaging	Het

Other mutations in Ncam1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00592:Ncam1	APN	9	49,434,865 (GRCm39)	missense	probably damaging	1.00
IGL01384:Ncam1	APN	9	49,421,152 (GRCm39)	missense	possibly damaging	0.76
IGL01798:Ncam1	APN	9	49,419,907 (GRCm39)	missense	probably damaging	1.00
IGL02239:Ncam1	APN	9	49,478,702 (GRCm39)	missense	probably damaging	1.00
IGL02368:Ncam1	APN	9	49,454,383 (GRCm39)	nonsense	probably null
IGL02616:Ncam1	APN	9	49,419,988 (GRCm39)	missense	probably benign	0.23
PIT4431001:Ncam1	UTSW	9	49,709,993 (GRCm39)	missense	probably benign	0.04
R0164:Ncam1	UTSW	9	49,479,709 (GRCm39)	missense	probably damaging	1.00
R0164:Ncam1	UTSW	9	49,479,709 (GRCm39)	missense	probably damaging	1.00
R0502:Ncam1	UTSW	9	49,481,118 (GRCm39)	unclassified	probably benign
R0924:Ncam1	UTSW	9	49,473,476 (GRCm39)	intron	probably benign
R1398:Ncam1	UTSW	9	49,428,889 (GRCm39)	intron	probably benign
R1440:Ncam1	UTSW	9	49,456,100 (GRCm39)	missense	probably damaging	1.00
R1491:Ncam1	UTSW	9	49,416,849 (GRCm39)	missense	probably benign	0.15
R1676:Ncam1	UTSW	9	49,468,472 (GRCm39)	missense	probably damaging	1.00
R1743:Ncam1	UTSW	9	49,468,445 (GRCm39)	missense	probably damaging	1.00
R1769:Ncam1	UTSW	9	49,456,556 (GRCm39)	unclassified	probably benign
R1951:Ncam1	UTSW	9	49,456,492 (GRCm39)	missense	probably benign	0.36
R2143:Ncam1	UTSW	9	49,454,319 (GRCm39)	missense	possibly damaging	0.87
R2167:Ncam1	UTSW	9	49,479,781 (GRCm39)	missense	probably benign	0.42
R2170:Ncam1	UTSW	9	49,709,981 (GRCm39)	missense	probably benign	0.06
R2290:Ncam1	UTSW	9	49,434,951 (GRCm39)	splice site	probably benign
R2321:Ncam1	UTSW	9	49,456,132 (GRCm39)	unclassified	probably benign
R3001:Ncam1	UTSW	9	49,468,526 (GRCm39)	missense	probably damaging	0.99
R3002:Ncam1	UTSW	9	49,468,526 (GRCm39)	missense	probably damaging	0.99
R4026:Ncam1	UTSW	9	49,476,295 (GRCm39)	missense	probably benign	0.00
R4279:Ncam1	UTSW	9	49,418,259 (GRCm39)	intron	probably benign
R4289:Ncam1	UTSW	9	49,468,472 (GRCm39)	missense	probably damaging	1.00
R4873:Ncam1	UTSW	9	49,418,921 (GRCm39)	intron	probably benign
R4875:Ncam1	UTSW	9	49,418,921 (GRCm39)	intron	probably benign
R4883:Ncam1	UTSW	9	49,453,183 (GRCm39)	splice site	probably null
R4899:Ncam1	UTSW	9	49,456,551 (GRCm39)	critical splice acceptor site	probably null
R4923:Ncam1	UTSW	9	49,416,779 (GRCm39)	missense	probably benign
R5041:Ncam1	UTSW	9	49,478,085 (GRCm39)	missense	probably damaging	1.00
R5058:Ncam1	UTSW	9	49,709,995 (GRCm39)	missense	probably benign	0.16
R5388:Ncam1	UTSW	9	49,456,054 (GRCm39)	missense	probably benign
R5512:Ncam1	UTSW	9	49,420,999 (GRCm39)	splice site	probably null
R5598:Ncam1	UTSW	9	49,457,051 (GRCm39)	missense	probably damaging	1.00
R5895:Ncam1	UTSW	9	49,418,343 (GRCm39)	missense	probably benign
R5972:Ncam1	UTSW	9	49,418,829 (GRCm39)	missense	possibly damaging	0.93
R6059:Ncam1	UTSW	9	49,455,966 (GRCm39)	missense	probably damaging	1.00
R6226:Ncam1	UTSW	9	49,476,304 (GRCm39)	missense	probably benign	0.00
R6392:Ncam1	UTSW	9	49,434,875 (GRCm39)	missense	probably damaging	0.99
R6750:Ncam1	UTSW	9	49,478,639 (GRCm39)	missense	probably damaging	1.00
R6799:Ncam1	UTSW	9	49,419,911 (GRCm39)	missense	probably damaging	0.99
R7230:Ncam1	UTSW	9	49,421,123 (GRCm39)	missense	probably benign	0.00
R7335:Ncam1	UTSW	9	49,418,211 (GRCm39)	missense
R7561:Ncam1	UTSW	9	49,476,242 (GRCm39)	missense	probably damaging	1.00
R7645:Ncam1	UTSW	9	49,476,303 (GRCm39)	missense	probably benign	0.01
R8022:Ncam1	UTSW	9	49,476,192 (GRCm39)	missense	possibly damaging	0.72
R8023:Ncam1	UTSW	9	49,421,057 (GRCm39)	missense	probably benign	0.00
R8045:Ncam1	UTSW	9	49,418,736 (GRCm39)	missense
R8234:Ncam1	UTSW	9	49,456,523 (GRCm39)	missense	probably damaging	0.99
R8308:Ncam1	UTSW	9	49,479,817 (GRCm39)	missense	probably damaging	0.99
R8370:Ncam1	UTSW	9	49,468,431 (GRCm39)	nonsense	probably null
R8500:Ncam1	UTSW	9	49,431,445 (GRCm39)	missense	probably damaging	1.00
R8542:Ncam1	UTSW	9	49,419,898 (GRCm39)	missense	probably damaging	1.00
R8944:Ncam1	UTSW	9	49,431,493 (GRCm39)	missense	probably damaging	1.00
R8977:Ncam1	UTSW	9	49,418,825 (GRCm39)	missense	probably damaging	1.00
R9028:Ncam1	UTSW	9	49,418,736 (GRCm39)	missense
R9034:Ncam1	UTSW	9	49,481,198 (GRCm39)	missense	probably benign	0.42
R9106:Ncam1	UTSW	9	49,428,856 (GRCm39)	missense	probably damaging	0.99
R9224:Ncam1	UTSW	9	49,419,995 (GRCm39)	missense	probably damaging	1.00
R9330:Ncam1	UTSW	9	49,456,097 (GRCm39)	missense	probably benign
X0062:Ncam1	UTSW	9	49,456,901 (GRCm39)	nonsense	probably null
X0064:Ncam1	UTSW	9	49,477,980 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGAGGGCTAAGTGGTTTCCTG -3'
(R):5'- ATGAACCTCTTCACCCCATG -3'

Sequencing Primer
(F):5'- GCTAAGTGGTTTCCTGGACTG -3'
(R):5'- CAGAATCATCACCCTTTGCG -3'

Posted On

2016-08-04