Incidental Mutation 'R3940:Calm5'
ID474751
Institutional Source Beutler Lab
Gene Symbol Calm5
Ensembl Gene ENSMUSG00000099269
Gene Namecalmodulin 5
SynonymsScarf2
MMRRC Submission 040922-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.109) question?
Stock #R3940 (G1)
Quality Score225
Status Not validated
Chromosome13
Chromosomal Location3854173-3854761 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 3854485 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 37 (I37F)
Ref Sequence ENSEMBL: ENSMUSP00000139172 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000183198] [ENSMUST00000184271]
Predicted Effect possibly damaging
Transcript: ENSMUST00000183198
AA Change: I37F

PolyPhen 2 Score 0.872 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000139172
Gene: ENSMUSG00000099269
AA Change: I37F

DomainStartEndE-ValueType
EFh 12 40 1.9e-7 SMART
EFh 45 73 6.7e-10 SMART
EFh 81 109 1.5e-2 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000184271
AA Change: I60F

PolyPhen 2 Score 0.704 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000139342
Gene: ENSMUSG00000099269
AA Change: I60F

DomainStartEndE-ValueType
EFh 35 63 3.7e-5 SMART
EFh 68 96 1.35e-7 SMART
EFh 104 132 3.12e0 SMART
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.5%
Validation Efficiency 95% (35/37)
Allele List at MGI
Other mutations in this stock
Total: 34 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930567H17Rik C T X: 70,394,529 A53T probably benign Het
Acsm3 A G 7: 119,773,886 E204G probably benign Het
Acta2 A T 19: 34,243,480 I276N possibly damaging Het
Ankrd16 T C 2: 11,784,381 C260R probably benign Het
Ankrd42 T C 7: 92,591,788 probably null Het
Atp13a2 T C 4: 141,006,422 S1041P probably damaging Het
Brinp3 C A 1: 146,751,861 D277E probably damaging Het
Casq1 A G 1: 172,219,536 V52A possibly damaging Het
Col22a1 A T 15: 71,981,933 L260* probably null Het
Cttnbp2 C A 6: 18,420,975 V846L probably benign Het
Dnah12 A G 14: 26,723,599 T627A probably benign Het
Eogt T C 6: 97,113,914 I421M probably damaging Het
Fam135a T A 1: 24,057,475 H63L probably damaging Het
Fmo3 T A 1: 162,963,986 T241S probably benign Het
Frem3 T C 8: 80,615,020 I1314T possibly damaging Het
Gm14393 T C 2: 175,061,627 probably null Het
Kcna5 C T 6: 126,533,651 V505I probably damaging Het
Kit A G 5: 75,609,318 D130G probably benign Het
Neto2 G A 8: 85,674,118 T16I probably damaging Het
Olfr1090 T A 2: 86,753,931 D269V possibly damaging Het
Olfr57 T A 10: 79,035,204 I136N probably damaging Het
Pcdhb7 T C 18: 37,343,968 L719P probably damaging Het
Pcdhga9 G A 18: 37,738,942 R608H probably benign Het
Pik3ip1 A G 11: 3,331,987 N48S probably damaging Het
Pkn2 G A 3: 142,793,911 S951L probably damaging Het
Prrc2a T C 17: 35,157,498 H772R possibly damaging Het
Ric1 T C 19: 29,570,762 Y277H probably damaging Het
Rin2 C T 2: 145,860,446 T354I probably benign Het
Rnf123 A T 9: 108,064,035 probably benign Het
Robo1 T C 16: 73,009,743 S1166P probably benign Het
S100a10 A G 3: 93,561,076 E38G probably benign Het
Slc34a1 A T 13: 55,413,170 I483F probably damaging Het
Stim1 A G 7: 102,435,641 N600S probably benign Het
Ube3c T A 5: 29,619,360 N517K probably benign Het
Other mutations in Calm5
AlleleSourceChrCoordTypePredicted EffectPPH Score
R3732:Calm5 UTSW 13 3854337 missense probably damaging 1.00
R4563:Calm5 UTSW 13 3854402 nonsense probably null
R4793:Calm5 UTSW 13 3854401 missense probably benign 0.06
R5585:Calm5 UTSW 13 3854372 missense possibly damaging 0.71
R5775:Calm5 UTSW 13 3854435 missense probably damaging 0.99
R5869:Calm5 UTSW 13 3854321 splice site probably benign
R6125:Calm5 UTSW 13 3854491 nonsense probably null
Predicted Primers
Posted On2017-04-14