Mutagenetix > Incidental Mutation

Incidental Mutation 'R4257:Gm9755'

321864

Institutional Source

Beutler Lab

Gene Symbol

Gm9755

Ensembl Gene

ENSMUSG00000030735

Gene Name

predicted pseudogene 9755

Synonyms

MMRRC Submission

041070-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.923) question?

Stock #

R4257 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67966887-67968242 bp(-) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

A to T at 67967129 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000032981

SMART Domains

Protein: ENSMUSP00000091180
Gene: ENSMUSG00000030735

Domain	Start	End	E-Value	Type
Pfam:GTP_EFTU	55	249	1.4e-55	PFAM
Pfam:GTP_EFTU_D2	272	341	4.8e-17	PFAM
Pfam:GTP_EFTU_D3	345	440	1.5e-25	PFAM

Meta Mutation Damage Score

0.0579

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

100% (39/39)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930451I11Rik	C	T	7: 126,430,662 (GRCm39)		probably benign	Het
4930578I06Rik	C	T	14: 64,210,658 (GRCm39)	R190H	probably benign	Het
Akap13	T	A	7: 75,261,033 (GRCm39)	I1219K	probably damaging	Het
Arfgef1	T	C	1: 10,229,771 (GRCm39)		probably benign	Het
Arhgap24	A	G	5: 102,811,983 (GRCm39)	E70G	probably benign	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Babam2	T	C	5: 31,859,414 (GRCm39)	S40P	possibly damaging	Het
Brwd1	A	G	16: 95,824,696 (GRCm39)	V1190A	probably damaging	Het
Ccpg1	A	G	9: 72,919,909 (GRCm39)	E508G	probably damaging	Het
Ckm	T	C	7: 19,155,279 (GRCm39)	S372P	probably benign	Het
Egflam	T	A	15: 7,283,907 (GRCm39)		probably null	Het
Farp1	G	A	14: 121,492,891 (GRCm39)	V498M	probably benign	Het
Galnt14	T	A	17: 73,811,899 (GRCm39)	I441F	probably benign	Het
Gm5414	A	G	15: 101,533,107 (GRCm39)	L440P	probably damaging	Het
Gm6563	A	G	19: 23,653,339 (GRCm39)	E43G	possibly damaging	Het
Gmds	A	G	13: 32,004,172 (GRCm39)	S337P	possibly damaging	Het
L3mbtl3	T	A	10: 26,156,020 (GRCm39)	Q754L	unknown	Het
Ltk	G	A	2: 119,583,485 (GRCm39)	T300I	possibly damaging	Het
Or5d46	A	C	2: 88,170,621 (GRCm39)	K237N	probably damaging	Het
Pbx2	C	A	17: 34,813,619 (GRCm39)	H184Q	probably damaging	Het
Plxna2	T	C	1: 194,327,083 (GRCm39)	F339S	probably damaging	Het
Prkaa2	A	T	4: 104,897,153 (GRCm39)	D353E	probably benign	Het
Prss36	G	A	7: 127,532,010 (GRCm39)		probably benign	Het
Rimbp2	A	G	5: 128,851,324 (GRCm39)	V874A	probably damaging	Het
Rspo2	C	A	15: 42,939,307 (GRCm39)	R161L	probably benign	Het
Ryr1	T	C	7: 28,781,875 (GRCm39)	D2038G	possibly damaging	Het
Stkld1	A	G	2: 26,833,146 (GRCm39)	M111V	probably benign	Het
Tprn	A	G	2: 25,154,494 (GRCm39)	I599V	probably damaging	Het
Upp2	A	T	2: 58,670,106 (GRCm39)	I219F	probably damaging	Het
Vmn2r94	A	T	17: 18,464,433 (GRCm39)	F619Y	probably damaging	Het
Xirp2	A	G	2: 67,346,383 (GRCm39)	T2875A	probably benign	Het
Zfp64	A	G	2: 168,768,298 (GRCm39)	L438P	probably damaging	Het

Other mutations in Gm9755

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL01655:Gm9755	APN	8	67,967,885 (GRCm39)	exon	noncoding transcript
IGL03217:Gm9755	APN	8	67,967,035 (GRCm39)	exon	noncoding transcript
IGL03229:Gm9755	APN	8	67,967,324 (GRCm39)	exon	noncoding transcript
R1110:Gm9755	UTSW	8	67,967,710 (GRCm39)	exon	noncoding transcript
R1630:Gm9755	UTSW	8	67,967,312 (GRCm39)	exon	noncoding transcript
R4743:Gm9755	UTSW	8	67,966,929 (GRCm39)	exon	noncoding transcript
R5960:Gm9755	UTSW	8	67,967,840 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TGGTACATCAGTAACAAGGCC -3'
(R):5'- GATGTTCCACAAGAGCCTGG -3'

Sequencing Primer
(F):5'- GCCAGTGCCAATGGTCTTG -3'
(R):5'- TTCCACAAGAGCCTGGAGAGG -3'

Posted On

2015-06-20