Incidental Mutation 'R6464:Arhgef10l'
| ID |
517727 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Arhgef10l
|
| Ensembl Gene |
ENSMUSG00000040964 |
| Gene Name |
Rho guanine nucleotide exchange factor 10-like |
| Synonyms |
2810441C07Rik |
| MMRRC Submission |
044597-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.101)
|
| Stock # |
R6464 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
4 |
| Chromosomal Location |
140241796-140393318 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to C
at 140314126 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Arginine
at position 230
(M230R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000101425
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000039204]
[ENSMUST00000069623]
[ENSMUST00000097820]
[ENSMUST00000105797]
[ENSMUST00000105798]
[ENSMUST00000105799]
|
| AlphaFold |
A2AWP8 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000039204
AA Change: M230R
PolyPhen 2
Score 0.046 (Sensitivity: 0.94; Specificity: 0.83)
|
| SMART Domains |
Protein: ENSMUSP00000040531
Gene: ENSMUSG00000040964
AA Change: M230R
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
10 |
25 |
N/A |
INTRINSIC |
|
low complexity region
|
28 |
49 |
N/A |
INTRINSIC |
|
low complexity region
|
78 |
89 |
N/A |
INTRINSIC |
|
low complexity region
|
105 |
116 |
N/A |
INTRINSIC |
|
low complexity region
|
170 |
184 |
N/A |
INTRINSIC |
|
RhoGEF
|
318 |
500 |
1.95e-52 |
SMART |
|
Blast:PH
|
535 |
748 |
3e-82 |
BLAST |
|
low complexity region
|
821 |
833 |
N/A |
INTRINSIC |
|
low complexity region
|
864 |
876 |
N/A |
INTRINSIC |
|
Blast:WD40
|
1217 |
1270 |
8e-18 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000069623
|
| SMART Domains |
Protein: ENSMUSP00000066249
Gene: ENSMUSG00000040964
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
10 |
25 |
N/A |
INTRINSIC |
|
low complexity region
|
28 |
49 |
N/A |
INTRINSIC |
|
low complexity region
|
78 |
89 |
N/A |
INTRINSIC |
|
low complexity region
|
105 |
116 |
N/A |
INTRINSIC |
|
low complexity region
|
170 |
184 |
N/A |
INTRINSIC |
|
RhoGEF
|
279 |
461 |
1.95e-52 |
SMART |
|
Blast:PH
|
496 |
714 |
5e-80 |
BLAST |
|
low complexity region
|
787 |
799 |
N/A |
INTRINSIC |
|
low complexity region
|
830 |
842 |
N/A |
INTRINSIC |
|
Blast:WD40
|
1183 |
1236 |
7e-18 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000097820
|
| SMART Domains |
Protein: ENSMUSP00000095431
Gene: ENSMUSG00000040964
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
10 |
25 |
N/A |
INTRINSIC |
|
low complexity region
|
28 |
49 |
N/A |
INTRINSIC |
|
low complexity region
|
78 |
89 |
N/A |
INTRINSIC |
|
low complexity region
|
105 |
116 |
N/A |
INTRINSIC |
|
low complexity region
|
170 |
184 |
N/A |
INTRINSIC |
|
RhoGEF
|
279 |
461 |
1.95e-52 |
SMART |
|
Blast:PH
|
496 |
709 |
3e-82 |
BLAST |
|
low complexity region
|
782 |
794 |
N/A |
INTRINSIC |
|
low complexity region
|
825 |
837 |
N/A |
INTRINSIC |
|
Blast:WD40
|
1178 |
1231 |
6e-18 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000105797
|
| SMART Domains |
Protein: ENSMUSP00000101423
Gene: ENSMUSG00000040964
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
50 |
62 |
N/A |
INTRINSIC |
|
Pfam:RhoGEF
|
101 |
183 |
7.1e-15 |
PFAM |
|
low complexity region
|
195 |
213 |
N/A |
INTRINSIC |
|
Blast:PH
|
248 |
461 |
7e-83 |
BLAST |
|
low complexity region
|
534 |
546 |
N/A |
INTRINSIC |
|
low complexity region
|
577 |
589 |
N/A |
INTRINSIC |
|
Blast:WD40
|
618 |
656 |
6e-15 |
BLAST |
|
Blast:WD40
|
930 |
983 |
1e-17 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000105798
|
| SMART Domains |
Protein: ENSMUSP00000101424
Gene: ENSMUSG00000040964
| Domain | Start | End | E-Value | Type |
|---|
|
RhoGEF
|
78 |
260 |
1.95e-52 |
SMART |
|
Blast:PH
|
295 |
513 |
8e-81 |
BLAST |
|
low complexity region
|
586 |
598 |
N/A |
INTRINSIC |
|
low complexity region
|
629 |
641 |
N/A |
INTRINSIC |
|
Blast:WD40
|
982 |
1035 |
6e-18 |
BLAST |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000105799
AA Change: M230R
PolyPhen 2
Score 0.046 (Sensitivity: 0.94; Specificity: 0.83)
|
| SMART Domains |
Protein: ENSMUSP00000101425
Gene: ENSMUSG00000040964
AA Change: M230R
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
10 |
25 |
N/A |
INTRINSIC |
|
low complexity region
|
28 |
49 |
N/A |
INTRINSIC |
|
low complexity region
|
78 |
89 |
N/A |
INTRINSIC |
|
low complexity region
|
105 |
116 |
N/A |
INTRINSIC |
|
low complexity region
|
170 |
184 |
N/A |
INTRINSIC |
|
RhoGEF
|
318 |
500 |
1.95e-52 |
SMART |
|
Blast:PH
|
535 |
753 |
5e-80 |
BLAST |
|
low complexity region
|
826 |
838 |
N/A |
INTRINSIC |
|
low complexity region
|
869 |
881 |
N/A |
INTRINSIC |
|
Blast:WD40
|
1222 |
1275 |
8e-18 |
BLAST |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000125045
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 97.9%
- 20x: 93.8%
|
| Validation Efficiency |
100% (48/48) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene belongs to the RhoGEF subfamily of RhoGTPases. Members of this subfamily are activated by specific guanine nucleotide exchange factors (GEFs) and are involved in signal transduction. The encoded protein shows cytosolic distribution. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2016]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 51 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcc1 |
T |
A |
16: 14,265,354 (GRCm39) |
S769T |
probably damaging |
Het |
| Apoe |
G |
A |
7: 19,431,461 (GRCm39) |
T52M |
probably damaging |
Het |
| Cdkl4 |
A |
T |
17: 80,832,781 (GRCm39) |
I321N |
probably benign |
Het |
| Ceacam5 |
T |
C |
7: 17,481,391 (GRCm39) |
|
probably null |
Het |
| Cfap70 |
T |
C |
14: 20,451,107 (GRCm39) |
|
probably null |
Het |
| Clasp2 |
A |
G |
9: 113,602,785 (GRCm39) |
Y195C |
probably damaging |
Het |
| Clip2 |
T |
C |
5: 134,520,779 (GRCm39) |
I999V |
probably benign |
Het |
| Cntnap5a |
A |
G |
1: 116,112,138 (GRCm39) |
D476G |
probably benign |
Het |
| Col6a6 |
C |
T |
9: 105,666,152 (GRCm39) |
M1I |
probably null |
Het |
| Cyp27a1 |
T |
C |
1: 74,775,047 (GRCm39) |
V292A |
possibly damaging |
Het |
| Cyp2c29 |
A |
G |
19: 39,317,669 (GRCm39) |
Y385C |
probably damaging |
Het |
| Dsg3 |
A |
C |
18: 20,666,583 (GRCm39) |
R597S |
probably benign |
Het |
| Fam131c |
T |
C |
4: 141,109,653 (GRCm39) |
I95T |
probably damaging |
Het |
| Fam13b |
A |
T |
18: 34,606,684 (GRCm39) |
C302* |
probably null |
Het |
| Fkbp15 |
T |
C |
4: 62,226,315 (GRCm39) |
K745E |
possibly damaging |
Het |
| Flg |
T |
A |
3: 93,188,688 (GRCm39) |
|
probably benign |
Het |
| Gm11559 |
T |
C |
11: 99,755,542 (GRCm39) |
C64R |
unknown |
Het |
| Helt |
T |
C |
8: 46,745,571 (GRCm39) |
D104G |
probably damaging |
Het |
| Helz2 |
T |
C |
2: 180,876,862 (GRCm39) |
T1211A |
probably benign |
Het |
| Ifna9 |
T |
C |
4: 88,510,487 (GRCm39) |
R46G |
possibly damaging |
Het |
| Kif20b |
G |
A |
19: 34,911,841 (GRCm39) |
V235I |
probably benign |
Het |
| Kif7 |
A |
G |
7: 79,363,842 (GRCm39) |
V22A |
possibly damaging |
Het |
| Klra9 |
G |
T |
6: 130,155,995 (GRCm39) |
Y253* |
probably null |
Het |
| Lipf |
A |
T |
19: 33,950,944 (GRCm39) |
Y305F |
probably benign |
Het |
| Lpar1 |
A |
G |
4: 58,486,875 (GRCm39) |
L132P |
possibly damaging |
Het |
| Magi1 |
A |
T |
6: 93,676,770 (GRCm39) |
V834E |
probably damaging |
Het |
| Meltf |
T |
C |
16: 31,709,594 (GRCm39) |
Y432H |
probably benign |
Het |
| Mroh2a |
G |
C |
1: 88,185,524 (GRCm39) |
E1510D |
probably damaging |
Het |
| Myo1f |
T |
C |
17: 33,795,621 (GRCm39) |
L59P |
probably damaging |
Het |
| Naa25 |
G |
A |
5: 121,556,024 (GRCm39) |
D271N |
probably damaging |
Het |
| Or10ag55-ps1 |
A |
T |
2: 87,139,951 (GRCm39) |
I273F |
probably damaging |
Het |
| Or8u3-ps |
A |
T |
2: 85,952,752 (GRCm39) |
I162F |
possibly damaging |
Het |
| Otoa |
A |
G |
7: 120,701,828 (GRCm39) |
Q169R |
probably damaging |
Het |
| Pde4dip |
C |
T |
3: 97,617,660 (GRCm39) |
D1723N |
probably damaging |
Het |
| Ptdss2 |
T |
C |
7: 140,732,124 (GRCm39) |
V175A |
probably damaging |
Het |
| Rdh8 |
G |
T |
9: 20,734,696 (GRCm39) |
R121L |
probably damaging |
Het |
| Rpl23 |
T |
C |
11: 97,669,111 (GRCm39) |
|
probably null |
Het |
| Scn5a |
T |
C |
9: 119,363,646 (GRCm39) |
D498G |
probably damaging |
Het |
| Slc39a14 |
G |
C |
14: 70,544,177 (GRCm39) |
L470V |
probably damaging |
Het |
| Smok2a |
C |
T |
17: 13,445,317 (GRCm39) |
A298V |
probably damaging |
Het |
| Tesk2 |
A |
C |
4: 116,660,046 (GRCm39) |
D388A |
probably damaging |
Het |
| Tnik |
G |
A |
3: 28,666,119 (GRCm39) |
|
probably null |
Het |
| Ube3a |
T |
A |
7: 58,925,931 (GRCm39) |
Y236* |
probably null |
Het |
| Ugt1a1 |
CAGAGAGAGAGAGA |
CAGAGAGAGAGA |
1: 88,139,706 (GRCm39) |
|
probably benign |
Het |
| Vinac1 |
T |
C |
2: 128,881,465 (GRCm39) |
T154A |
possibly damaging |
Het |
| Vmn1r74 |
A |
T |
7: 11,581,131 (GRCm39) |
I144L |
possibly damaging |
Het |
| Vmn2r1 |
G |
A |
3: 64,008,766 (GRCm39) |
D482N |
probably benign |
Het |
| Vwf |
T |
C |
6: 125,616,363 (GRCm39) |
|
probably null |
Het |
| Wdr97 |
A |
G |
15: 76,246,977 (GRCm39) |
T1413A |
probably benign |
Het |
| Zfp703 |
C |
G |
8: 27,469,355 (GRCm39) |
P340A |
probably damaging |
Het |
| Znfx1 |
T |
C |
2: 166,888,842 (GRCm39) |
S789G |
probably benign |
Het |
|
| Other mutations in Arhgef10l |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01420:Arhgef10l
|
APN |
4 |
140,297,649 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL01732:Arhgef10l
|
APN |
4 |
140,307,726 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL01988:Arhgef10l
|
APN |
4 |
140,305,672 (GRCm39) |
splice site |
probably benign |
|
|
IGL02031:Arhgef10l
|
APN |
4 |
140,302,656 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02253:Arhgef10l
|
APN |
4 |
140,271,595 (GRCm39) |
nonsense |
probably null |
|
|
IGL02445:Arhgef10l
|
APN |
4 |
140,274,318 (GRCm39) |
missense |
probably benign |
0.19 |
|
IGL02619:Arhgef10l
|
APN |
4 |
140,321,504 (GRCm39) |
missense |
probably benign |
0.07 |
|
IGL02798:Arhgef10l
|
APN |
4 |
140,292,441 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL03064:Arhgef10l
|
APN |
4 |
140,306,590 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03178:Arhgef10l
|
APN |
4 |
140,271,739 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
IGL03236:Arhgef10l
|
APN |
4 |
140,338,671 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03352:Arhgef10l
|
APN |
4 |
140,311,242 (GRCm39) |
start codon destroyed |
probably null |
0.99 |
|
PIT4494001:Arhgef10l
|
UTSW |
4 |
140,292,522 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R0057:Arhgef10l
|
UTSW |
4 |
140,338,529 (GRCm39) |
splice site |
probably benign |
|
|
R0062:Arhgef10l
|
UTSW |
4 |
140,279,843 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0109:Arhgef10l
|
UTSW |
4 |
140,305,605 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0109:Arhgef10l
|
UTSW |
4 |
140,305,605 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0114:Arhgef10l
|
UTSW |
4 |
140,311,194 (GRCm39) |
missense |
probably benign |
0.17 |
|
R0334:Arhgef10l
|
UTSW |
4 |
140,311,237 (GRCm39) |
nonsense |
probably null |
|
|
R0742:Arhgef10l
|
UTSW |
4 |
140,264,156 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1017:Arhgef10l
|
UTSW |
4 |
140,242,617 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1166:Arhgef10l
|
UTSW |
4 |
140,302,581 (GRCm39) |
unclassified |
probably benign |
|
|
R1397:Arhgef10l
|
UTSW |
4 |
140,271,754 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1521:Arhgef10l
|
UTSW |
4 |
140,242,749 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R1707:Arhgef10l
|
UTSW |
4 |
140,291,600 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1793:Arhgef10l
|
UTSW |
4 |
140,242,684 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R2018:Arhgef10l
|
UTSW |
4 |
140,271,695 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2093:Arhgef10l
|
UTSW |
4 |
140,297,601 (GRCm39) |
missense |
possibly damaging |
0.57 |
|
R2098:Arhgef10l
|
UTSW |
4 |
140,306,743 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2310:Arhgef10l
|
UTSW |
4 |
140,320,429 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2879:Arhgef10l
|
UTSW |
4 |
140,242,598 (GRCm39) |
missense |
probably benign |
0.09 |
|
R2883:Arhgef10l
|
UTSW |
4 |
140,244,113 (GRCm39) |
missense |
probably benign |
0.02 |
|
R3732:Arhgef10l
|
UTSW |
4 |
140,308,930 (GRCm39) |
small deletion |
probably benign |
|
|
R3732:Arhgef10l
|
UTSW |
4 |
140,308,930 (GRCm39) |
small deletion |
probably benign |
|
|
R3861:Arhgef10l
|
UTSW |
4 |
140,242,798 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R4049:Arhgef10l
|
UTSW |
4 |
140,242,762 (GRCm39) |
missense |
probably benign |
0.05 |
|
R4322:Arhgef10l
|
UTSW |
4 |
140,270,037 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4707:Arhgef10l
|
UTSW |
4 |
140,264,194 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R5395:Arhgef10l
|
UTSW |
4 |
140,297,601 (GRCm39) |
missense |
probably benign |
0.16 |
|
R5720:Arhgef10l
|
UTSW |
4 |
140,308,930 (GRCm39) |
small deletion |
probably benign |
|
|
R6066:Arhgef10l
|
UTSW |
4 |
140,304,391 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6190:Arhgef10l
|
UTSW |
4 |
140,270,073 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R6476:Arhgef10l
|
UTSW |
4 |
140,338,693 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6478:Arhgef10l
|
UTSW |
4 |
140,270,068 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R6483:Arhgef10l
|
UTSW |
4 |
140,344,226 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6631:Arhgef10l
|
UTSW |
4 |
140,245,058 (GRCm39) |
intron |
probably benign |
|
|
R6721:Arhgef10l
|
UTSW |
4 |
140,297,655 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6890:Arhgef10l
|
UTSW |
4 |
140,271,730 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7098:Arhgef10l
|
UTSW |
4 |
140,308,222 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7100:Arhgef10l
|
UTSW |
4 |
140,244,126 (GRCm39) |
missense |
possibly damaging |
0.60 |
|
R7117:Arhgef10l
|
UTSW |
4 |
140,291,497 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7195:Arhgef10l
|
UTSW |
4 |
140,338,721 (GRCm39) |
missense |
probably benign |
|
|
R7222:Arhgef10l
|
UTSW |
4 |
140,248,580 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7397:Arhgef10l
|
UTSW |
4 |
140,290,115 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7776:Arhgef10l
|
UTSW |
4 |
140,302,642 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7801:Arhgef10l
|
UTSW |
4 |
140,271,578 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7811:Arhgef10l
|
UTSW |
4 |
140,242,335 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R7832:Arhgef10l
|
UTSW |
4 |
140,305,616 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R7849:Arhgef10l
|
UTSW |
4 |
140,311,245 (GRCm39) |
critical splice acceptor site |
probably null |
|
|
R7963:Arhgef10l
|
UTSW |
4 |
140,306,736 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8434:Arhgef10l
|
UTSW |
4 |
140,291,582 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R8943:Arhgef10l
|
UTSW |
4 |
140,292,550 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9004:Arhgef10l
|
UTSW |
4 |
140,279,921 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9006:Arhgef10l
|
UTSW |
4 |
140,271,659 (GRCm39) |
missense |
probably benign |
0.04 |
|
R9033:Arhgef10l
|
UTSW |
4 |
140,321,463 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9302:Arhgef10l
|
UTSW |
4 |
140,242,548 (GRCm39) |
missense |
probably benign |
0.04 |
|
R9337:Arhgef10l
|
UTSW |
4 |
140,338,624 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9375:Arhgef10l
|
UTSW |
4 |
140,319,265 (GRCm39) |
missense |
probably benign |
|
|
R9454:Arhgef10l
|
UTSW |
4 |
140,308,236 (GRCm39) |
nonsense |
probably null |
|
|
Z1088:Arhgef10l
|
UTSW |
4 |
140,309,046 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
Z1177:Arhgef10l
|
UTSW |
4 |
140,244,083 (GRCm39) |
missense |
probably benign |
0.28 |
|
| Predicted Primers |
PCR Primer
(F):5'- AACAGTTGGCTCATGGCTCC -3'
(R):5'- ACCCAGACTGCTATCCTGTC -3'
Sequencing Primer
(F):5'- TCCGCCCTCCTGCAGTG -3'
(R):5'- GTCTGCCATGTGACCTGAGTC -3'
|
| Posted On |
2018-05-21 |
Incidental Mutation