Mutagenetix > Incidental Mutation

Incidental Mutation 'R8432:Zfp931'

653786

Institutional Source

Beutler Lab

Gene Symbol

Zfp931

Ensembl Gene

ENSMUSG00000078861

Gene Name

zinc finger protein 931

Synonyms

2810021G02Rik

MMRRC Submission

067882-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.113) question?

Stock #

R8432 (G1)

Quality Score

80.0075

Status

Validated

Chromosome

Chromosomal Location

177709488-177720269 bp(-) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

A to G at 177711346 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Glutamine at position 65 (*65Q)

Ref Sequence

ENSEMBL: ENSMUSP00000104551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108923] [ENSMUST00000108924] [ENSMUST00000131702]

AlphaFold

A2AHM2

Predicted Effect

probably null
Transcript: ENSMUST00000108923
AA Change: *65Q

SMART Domains

Protein: ENSMUSP00000104551
Gene: ENSMUSG00000078861
AA Change: *65Q

Domain	Start	End	E-Value	Type
KRAB	4	64	2.27e-13	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108924

SMART Domains

Protein: ENSMUSP00000104552
Gene: ENSMUSG00000078861

Domain	Start	End	E-Value	Type
KRAB	4	66	3.04e-14	SMART
ZnF_C2H2	78	97	2.63e2	SMART
ZnF_C2H2	103	125	1.2e-3	SMART
ZnF_C2H2	131	153	8.02e-5	SMART
ZnF_C2H2	159	181	2.12e-4	SMART
ZnF_C2H2	187	209	1.4e-4	SMART
ZnF_C2H2	215	237	2.99e-4	SMART
ZnF_C2H2	243	265	4.87e-4	SMART
ZnF_C2H2	271	293	7.49e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131702

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

98% (61/62)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd5	A	G	9: 122,197,317 (GRCm39)	Y168C	probably damaging	Het
Agbl1	A	C	7: 76,774,434 (GRCm39)	R1119S	unknown	Het
Agpat5	A	T	8: 18,896,777 (GRCm39)	N68Y	probably benign	Het
Ap4b1	G	A	3: 103,728,135 (GRCm39)	R551Q	probably benign	Het
Arhgef28	T	C	13: 98,088,091 (GRCm39)	I1122V	probably benign	Het
Arhgef40	T	A	14: 52,226,857 (GRCm39)	D300E	probably benign	Het
Atxn7	T	C	14: 14,013,635 (GRCm38)	V112A	probably benign	Het
AY358078	T	A	14: 52,059,635 (GRCm39)	L324H	unknown	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Bnip5	C	T	17: 29,118,545 (GRCm39)	R629H	probably benign	Het
Cacng2	A	G	15: 77,897,522 (GRCm39)	Y96H	probably damaging	Het
Cd2ap	A	T	17: 43,109,484 (GRCm39)		probably null	Het
Cdh5	A	T	8: 104,839,698 (GRCm39)	E56D	probably damaging	Het
Cfap65	C	T	1: 74,967,203 (GRCm39)	G249R	probably benign	Het
Cpne3	A	T	4: 19,535,227 (GRCm39)	S268R	probably benign	Het
Cubn	T	A	2: 13,386,610 (GRCm39)	I1552F	probably benign	Het
Dhrs7	G	A	12: 72,711,581 (GRCm39)		probably benign	Het
Dnah7a	G	C	1: 53,657,195 (GRCm39)	I860M	possibly damaging	Het
Dync1h1	A	T	12: 110,584,576 (GRCm39)	M660L	probably benign	Het
Ecd	T	A	14: 20,370,998 (GRCm39)	T574S	probably benign	Het
Efna5	A	G	17: 62,958,017 (GRCm39)	Y80H	probably damaging	Het
Ercc5	A	T	1: 44,206,841 (GRCm39)	K585*	probably null	Het
Erich4	A	G	7: 25,314,533 (GRCm39)	S127P	probably benign	Het
Gckr	A	T	5: 31,466,447 (GRCm39)	Q474L	possibly damaging	Het
Gid8	A	G	2: 180,356,654 (GRCm39)	N97S	probably benign	Het
Gm28042	G	T	2: 119,869,077 (GRCm39)	C557F	probably damaging	Het
Gp2	C	T	7: 119,042,010 (GRCm39)	C505Y	probably benign	Het
Gzf1	A	G	2: 148,532,115 (GRCm39)	T581A	probably benign	Het
Heatr5b	A	T	17: 79,110,930 (GRCm39)	Y973N	probably damaging	Het
Herc2	A	G	7: 55,804,860 (GRCm39)	E2296G	probably benign	Het
Hydin	A	T	8: 111,324,583 (GRCm39)	N4648I	probably benign	Het
Mlip	A	T	9: 77,098,011 (GRCm39)	D157E	possibly damaging	Het
Mrpl19	A	G	6: 81,939,136 (GRCm39)	V222A	probably damaging	Het
Myo18b	A	G	5: 112,912,378 (GRCm39)	V1896A	probably benign	Het
Myo9a	G	A	9: 59,687,548 (GRCm39)	V218M	probably damaging	Het
Nepn	A	T	10: 52,267,880 (GRCm39)	T49S	probably benign	Het
Nsd1	T	G	13: 55,395,516 (GRCm39)	M1142R	possibly damaging	Het
Or52e8	T	G	7: 104,625,199 (GRCm39)	T2P	probably benign	Het
Or8b40	A	G	9: 38,027,272 (GRCm39)	Y65C	probably damaging	Het
Otulinl	A	T	15: 27,664,818 (GRCm39)	M46K	possibly damaging	Het
Pcnt	G	A	10: 76,256,039 (GRCm39)	R734W	probably damaging	Het
Pgm2l1	A	G	7: 99,909,260 (GRCm39)	D242G	possibly damaging	Het
Pkdrej	T	G	15: 85,701,494 (GRCm39)	I1481L	probably benign	Het
Prkag1	T	C	15: 98,713,425 (GRCm39)	I87V	possibly damaging	Het
Psma1	A	T	7: 113,873,080 (GRCm39)	I74N	probably damaging	Het
Rap1gds1	T	C	3: 138,647,548 (GRCm39)	S547G	probably damaging	Het
Rbm19	T	G	5: 120,313,991 (GRCm39)	F868V	probably damaging	Het
Riok1	T	C	13: 38,221,468 (GRCm39)	V11A	probably benign	Het
Smpd3	T	A	8: 106,984,309 (GRCm39)		probably null	Het
Taar3	A	T	10: 23,826,053 (GRCm39)	I200L	probably benign	Het
Taf12	A	G	4: 132,019,228 (GRCm39)	Y139C	probably damaging	Het
Taf15	AGAAGTGGAGGCTACGGTGGAGACCGAAGTGG	AGAAGTGG	11: 83,395,851 (GRCm39)		probably benign	Het
Tc2n	A	T	12: 101,615,363 (GRCm39)	N487K	probably benign	Het
Tex15	A	C	8: 34,066,572 (GRCm39)	I2001L	probably damaging	Het
Tmem168	A	G	6: 13,602,535 (GRCm39)	F277S	probably benign	Het
Trim67	TGCCGCCGCCGCCGC	TGCCGCCGCCGC	8: 125,520,801 (GRCm39)		probably benign	Het
Tspan1	G	T	4: 116,021,151 (GRCm39)	Q116K	probably benign	Het
Ttc24	G	A	3: 87,977,366 (GRCm39)	R325C	probably benign	Het
Ttn	C	T	2: 76,693,900 (GRCm39)	E323K		Het
Xirp2	A	T	2: 67,340,962 (GRCm39)	M1068L	probably benign	Het
Zbtb38	A	C	9: 96,568,291 (GRCm39)	M931R	possibly damaging	Het

Other mutations in Zfp931

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02980:Zfp931	APN	2	177,711,409 (GRCm39)	critical splice acceptor site	probably null
R1391:Zfp931	UTSW	2	177,709,984 (GRCm39)	missense	probably benign	0.01
R1907:Zfp931	UTSW	2	177,711,684 (GRCm39)	missense	probably damaging	1.00
R2109:Zfp931	UTSW	2	177,711,651 (GRCm39)	missense	probably null	1.00
R2283:Zfp931	UTSW	2	177,711,714 (GRCm39)	missense	possibly damaging	0.93
R4038:Zfp931	UTSW	2	177,709,777 (GRCm39)	missense	possibly damaging	0.53
R4039:Zfp931	UTSW	2	177,709,777 (GRCm39)	missense	possibly damaging	0.53
R4867:Zfp931	UTSW	2	177,709,855 (GRCm39)	missense	probably damaging	1.00
R4893:Zfp931	UTSW	2	177,709,996 (GRCm39)	missense	probably damaging	0.99
R5593:Zfp931	UTSW	2	177,709,595 (GRCm39)	missense	possibly damaging	0.47
R7295:Zfp931	UTSW	2	177,709,824 (GRCm39)	nonsense	probably null
R8050:Zfp931	UTSW	2	177,709,889 (GRCm39)	missense	probably damaging	1.00
R8069:Zfp931	UTSW	2	177,709,709 (GRCm39)	missense	probably benign	0.00
R8806:Zfp931	UTSW	2	177,709,589 (GRCm39)	missense	possibly damaging	0.66
R8866:Zfp931	UTSW	2	177,710,178 (GRCm39)	nonsense	probably null
R8987:Zfp931	UTSW	2	177,709,592 (GRCm39)	missense	probably damaging	1.00
R8987:Zfp931	UTSW	2	177,709,591 (GRCm39)	missense	probably damaging	1.00
R9088:Zfp931	UTSW	2	177,709,594 (GRCm39)	missense	probably damaging	1.00
R9538:Zfp931	UTSW	2	177,709,605 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TGGAATGTCTTGCAAACAACAG -3'
(R):5'- GGGCAGGACAGTCAGCAAAT -3'

Sequencing Primer
(F):5'- CTTGCAAACAACAGTTACATATCTGC -3'
(R):5'- AGTCACCTATGATGACGTGC -3'

Posted On

2020-10-20